Linear Mixed-Effects Models in chemistry: A tutorial
Anal Chim Acta. 2024 May 22;1304:342444. doi: 10.1016/j.aca.2024.342444. Epub 2024 Mar 27.ABSTRACTA common goal in chemistry is to study the relationship between a measured signal and the variability of certain factors. To this end, researchers often use Design of Experiment to decide which experiments to conduct and (Multiple) Linear Regression, and/or Analysis of Variance to analyze the collected data. Among the assumptions to the very foundation of this strategy, all the experiments are independent, conditional on the settings of the factors. Unfortunately, due to the presence of uncontrollable factors, real-life experi...
Source: Analytica Chimica Acta - April 18, 2024 Category: Chemistry Authors: Andrea Junior Carnoli Petra Oude Lohuis Lutgarde M C Buydens Gerjen H Tinnevelt Jeroen J Jansen Source Type: research
A sensitive and versatile electrochemical sensor based on hybridization chain reaction and CRISPR/Cas12a system for antibiotic detection
Anal Chim Acta. 2024 May 22;1304:342562. doi: 10.1016/j.aca.2024.342562. Epub 2024 Mar 30.ABSTRACTA sensitive electrochemical platform was constructed with NH2-Cu-MOF as electrochemical probe to detect antibiotics using CRISPR/Cas12a system triggered by hybridization chain reaction (HCR). The sensing system consists of two HCR systems. HCR1 occurred on the electrode surface independent of the target, generating long dsDNA to connect signal probes and producing a strong electrochemical signal. HCR2 was triggered by target, and the resulting dsDNA products activated the CRISPR/Cas12a, thereby resulting in effective and rapid...
Source: Analytica Chimica Acta - April 18, 2024 Category: Chemistry Authors: Xuemei Zhang Li Zhu Li Yang Guoyu Liu Shan Qiu Xiaoli Xiong Ke Huang Ting Xiao Liping Zhu Source Type: research
Energy difference-driven ROS reduction for electrochemical tracking crop growth sensitized with electron-migration nanostructures
Anal Chim Acta. 2024 May 22;1304:342515. doi: 10.1016/j.aca.2024.342515. Epub 2024 Mar 21.ABSTRACTAiming for sustainable crop productivity under changing climate conditions, it is essential to develop handy models for in-situ monitoring of reactive oxygen species (ROS). Herein, this work reports a simple electrochemical sensing toward hydrogen peroxide (H2O2) for tracking crop growth status sensitized with electron-migration nanostructure. To be specific, Cu-based metal-organic frameworks (MOFs) with high HOMO energy level are designed for H2O2 reduction on account of Cu(I)/Cu(II) redox switchability. Importantly, the sens...
Source: Analytica Chimica Acta - April 18, 2024 Category: Chemistry Authors: Xinai Zhang Yue Zhou Heng Wang Xiaowei Huang Yongqiang Shi Yucheng Zou Xuetao Hu Zhihua Li Jiyong Shi Xiaobo Zou Source Type: research
A novel self-enhanced ECL-RET aptasensor based on the bimetallic MOFs with homogeneous catalytic sites for kanamycin detection
In this study, we designed a new type of porphyrin metal-organic frameworks (Fe TCPP(Zn) MOFs) with homogeneous catalytic sites. The ferric-based metal ligands of Fe TCPP(Zn) MOFs acted as co-reaction accelerators, which effectively improved the conversion efficiency of H2O2 on the surface of MOFs, then increased the concentration of •OH surrounding porphyrin molecules to achieve self-enhanced electrochemiluminescence (ECL). Based on this, an aptasensor for the specific detection of kanamycin (KAN) in food and environmental water samples was constructed in combination with resonance energy transform (RET), in which Fe TC...
Source: Analytica Chimica Acta - April 18, 2024 Category: Chemistry Authors: Xuemei Wang Feifei Zhang Jianfei Xia Zhiyong Yan Zonghua Wang Source Type: research
Concomitant investigation of crustacean amphipods lipidome and metabolome during the molting cycle by Zeno SWATH data-independent acquisition coupled with electron activated dissociation and machine learning
Anal Chim Acta. 2024 May 22;1304:342533. doi: 10.1016/j.aca.2024.342533. Epub 2024 Apr 2.ABSTRACTBACKGROUND: DIA (Data-Independent Acquisition) is a powerful technique in Liquid Chromatography coupled with high-resolution tandem Mass Spectrometry (LC-MS/MS) initially developed for proteomics studies and recently emerging in metabolomics and lipidomics. It provides a comprehensive and unbiased coverage of molecules with improved reproducibility and quantitative accuracy compared to Data-Dependent Acquisition (DDA). Combined with the Zeno trap and Electron-Activated Dissociation (EAD), DIA enhances data quality and structura...
Source: Analytica Chimica Acta - April 18, 2024 Category: Chemistry Authors: Thomas Alexandre Brunet Yohann Cl ément Valentina Calabrese J érôme Lemoine Olivier Geffard Arnaud Chaumot Davide Degli-Esposti Arnaud Salvador Sophie Ayciriex Source Type: research
Absolute protein quantification based on calibrated particle counting using electrospray-differential mobility analysis
In this study, we developed an absolute quantification approach for proteins. This method is based on calibrated particle counting using electrospray-differential mobility analysis (ES-DMA) coupled with a condensation particle counter (CPC). The absolute concentration of proteins was quantified with the observed protein particle number measured with ES-DMA-CPC, and the detection efficiency was determined by calibrators. The measurement performance and quantitative level were verified using two certificated reference materials, BSA and NIMCmAb. The linear regression fit for the detection efficiency values of three reference...
Source: Analytica Chimica Acta - April 18, 2024 Category: Chemistry Authors: Wei Mi Xinyi Zhang Bin Wang Ruixue Sun Shangying Ma Zhishang Hu Xinhua Dai Source Type: research
Interplatform comparison between three ion mobility techniques for human plasma lipid collision cross sections
We present a comparison of CCS values of approximatively one hundred lipid species either commercially available or extracted from human plasma. We used three different commercial ion mobility technologies from different laboratories, drift tube IMS (DTIMS), travelling wave IMS (TWIMS) and trapped IMS (TIMS), to evaluate both instrument repeatability and interlaboratory reproducibility. We showed that CCS discrepancies of 0.3% (average) could occur depending on the data processing software tools. Moreover, eleven CCS calibrants were evaluated yielding mean RSD below 2% for eight calibrants, ESI Low concentration tuning mix...
Source: Analytica Chimica Acta - April 18, 2024 Category: Chemistry Authors: Ana ïs C George Isabelle Schmitz Florent Rouvi ère Sandra Alves Benoit Colsch Sabine Heinisch Carlos Afonso Fran çois Fenaille Corinne Loutelier-Bourhis Source Type: research
Scanning electrochemical microscopy for determining oxygen consumption rates of cells in hydrogel fibers fabricated using an extrusion 3D bioprinter
Anal Chim Acta. 2024 May 22;1304:342539. doi: 10.1016/j.aca.2024.342539. Epub 2024 Mar 28.ABSTRACTThree-dimensional (3D)-cultured cells have attracted the attention of researchers in tissue engineering- and drug screening-related fields. Among them, 3D cellular fibers have attracted significant attention because they can be stacked to prepare more complex tissues and organs. Cellular fibers are widely fabricated using extrusion 3D bioprinters. For these applications, it is necessary to evaluate cellular activities, such as the oxygen consumption rate (OCR), which is one of the major metabolic activities. We previously repo...
Source: Analytica Chimica Acta - April 18, 2024 Category: Chemistry Authors: Kosuke Ino Mana Wachi Yoshinobu Utagawa An Konno Masahiro Takinoue Hiroya Abe Hitoshi Shiku Source Type: research
Histidine tag modified magnetic beads for analysis of arsenic binding proteins
Anal Chim Acta. 2024 May 22;1304:342554. doi: 10.1016/j.aca.2024.342554. Epub 2024 Mar 29.ABSTRACTBACKGROUND: Many proteins with thiol groups can bind with trivalent arsenic which are termed as arsenic binding proteins, thus change their physiological functions. Therefore, it is vital to analyze the arsenic binding proteins in cells. The Pull-Down strategy based on biotinylated phenylarsenic acid (Bio-PAO(III)) probes is an effective way for analysis of arsenic binding proteins. In this strategy, streptavidin magnetic beads (SA-MBs) was applied to capture the arsenic binding proteins conjugating with Bio-PAO(III) probe. Ho...
Source: Analytica Chimica Acta - April 18, 2024 Category: Chemistry Authors: Kai Nan Man He Beibei Chen Bin Hu Source Type: research
A novel headspace solid-phase microextraction arrow method employing comprehensive two-dimensional gas chromatography-mass spectrometry combined with chemometric tools for the investigation of wine aging
Anal Chim Acta. 2024 May 22;1304:342555. doi: 10.1016/j.aca.2024.342555. Epub 2024 Mar 29.ABSTRACTBACKGROUND: Omics is used as an analytical tool to investigate wine authenticity issues. Aging authentication ensures that the wine has undergone the necessary maturation and developed its desired organoleptic characteristics. Considering that aged wines constitute valuable commodities, the development of advanced omics techniques that guarantee aging authenticity and prevent fraud is essential.RESULTS: Α solid phase microextraction Arrow method combined with comprehensive two-dimensional gas chromatography-mass spectrometry ...
Source: Analytica Chimica Acta - April 18, 2024 Category: Chemistry Authors: Natasa P Kalogiouri Natalia Manousi Antonio Ferracane George A Zachariadis Stefanos Koundouras Victoria F Samanidou Peter Q Tranchida Luigi Mondello Erwin Rosenberg Source Type: research
Portable IC system enabled with dual LED-based absorbance detectors and 3D-printed post-column heated micro-reactor for the simultaneous determination of ammonium, nitrite and nitrate
Anal Chim Acta. 2024 May 22;1304:342556. doi: 10.1016/j.aca.2024.342556. Epub 2024 Mar 28.ABSTRACTBACKGROUND: The on-site and simultaneous determination of anionic nitrite (NO2-) and nitrate (NO3-), and cationic ammonium (NH4+), in industrial and natural waters, presents a significant analytical challenge. Toward this end, herein a 3D-printed micro-reactor with an integrated heater chip was designed and optimised for the post-column colorimetric detection of NH4+ using a modified Berthelot reaction. The system was integrated within a portable and field deployable ion chromatograph (Aquamonitrix) designed to separate and de...
Source: Analytica Chimica Acta - April 18, 2024 Category: Chemistry Authors: Kurt Debruille Yonglin Mai Philip Hortin Simon Bluett Eoin Murray Vipul Gupta Brett Paull Source Type: research
Simultaneous separation and detection of monochloramine, nitrite, and nitrate by step-gradient mixed-mode ion chromatography: Translation from benchtop to portable ion chromatograph
Anal Chim Acta. 2024 May 22;1304:342557. doi: 10.1016/j.aca.2024.342557. Epub 2024 Mar 28.ABSTRACTBACKGROUND: Nitrite (NO2-) and nitrate (NO3-) can be produced in the distribution systems of chloraminated drinking water due to the nitrification of ammonia. The most applied inorganic chloramine for this purpose, namely monochloramine (NH2Cl), is also released into aquatic environments from water treatment plants' effluent and within industrial waste streams. Within the treatment process, the continuous monitoring of disinfectant levels is necessary to limit the harmful disinfectant by-product (DBP) formation. Currently, NH2...
Source: Analytica Chimica Acta - April 18, 2024 Category: Chemistry Authors: Ibraam E Mikhail Eoin Murray Simon Bluett Snezhana Astrakhantseva Brett Paull Source Type: research
A novel nanoparticle fluorescent probe based on a water-soluble conjugated polymer for real-time monitoring of ATP fluctuation and configuration of the Golgi apparatus during the inhibition of glycolysis
Anal Chim Acta. 2024 May 22;1304:342572. doi: 10.1016/j.aca.2024.342572. Epub 2024 Apr 2.ABSTRACTBACKGROUND: Adenosine 5'-triphosphate (ATP) plays an important role in cell metabolism and has been regarded as an indicator of cell survival and damage. Golgi apparatus participates in the signal transduction processes of substance transport, ion homeostasis and stress when extracellular substances enter cells. Till now, there is no fluorescent probe for monitoring Golgi ATP level fluctuation and visualizing the configuration change of the Golgi apparatus during the inhibition of glycolysis.RESULTS: Herein, we report the synth...
Source: Analytica Chimica Acta - April 18, 2024 Category: Chemistry Authors: Zhirong Geng Miaomiao Zhang Binghuan Huang Xiaohong Zhang Zhilin Wang Source Type: research
Efficient EVs separation and detection by an alumina-nanochannel-array-membrane integrated microfluidic chip and an antibody barcode biochip
Anal Chim Acta. 2024 May 22;1304:342576. doi: 10.1016/j.aca.2024.342576. Epub 2024 Apr 3.ABSTRACTBACKGROUND: Small endosome-derived lipid nanovesicles (30-200 nm) are actively secreted by living cells and serve as pivotal biomarkers for early cancer diagnosis. However, the study of extracellular vesicles (EVs) requires isolation and purification from various body fluids. Although traditional EVs isolation and detection technologies are mature, they usually require large amount of sample, consumes long-time, and have relatively low-throughput. How to efficiently isolate, purify and detect these structurally specific EVs fro...
Source: Analytica Chimica Acta - April 18, 2024 Category: Chemistry Authors: Jiaoyan Qiu Qindong Guo Yujin Chu Chunhua Wang Hao Xue Yu Zhang Hong Liu Gang Li Lin Han Source Type: research
Plasmon-enhanced fluorescence combined with aptamer sensor based on Ag nanocubes for signal-amplified detection of berberine hydrochloride
Anal Chim Acta. 2024 May 22;1304:342579. doi: 10.1016/j.aca.2024.342579. Epub 2024 Apr 4.ABSTRACTPlasmon enhanced fluorescent (PEF) with more "hot spots" play a critical role in signal amplified technology to avoid the intrinsic limitation of fluorophore which ascribed to a strong electromagnetic field at the tip structure. However, application of PEF technique to obtain a highly sensitive analysis of medicine was still at a very early stage. Herein, a simple but versatile Ag nanocubes (Agcubes)-based PEF sensor combined with aptamer (Agcubes@SiO2-QDs-Apt) was proposed for highly sensitive detection of berberine hydrochlor...
Source: Analytica Chimica Acta - April 18, 2024 Category: Chemistry Authors: Shilin Chen Chenye Xu Xingzhen Zhu Zhenghua Li Haoran Bie Yang Yang Jingtian Yu Yaqiong Yang He Huang Source Type: research
