Effects of Follicle Stimulating Hormone on Energy Balance and Tissue Metabolic Health After Loss of Ovarian Function
Am J Physiol Endocrinol Metab. 2024 Mar 27. doi: 10.1152/ajpendo.00400.2023. Online ahead of print.ABSTRACTLoss of ovarian function imparts increased susceptibility to obesity and metabolic disease. These effects are largely attributed to decreased estradiol (E2), but the role of increased follicle stimulating hormone (FSH) in modulating energy balance has not been fully investigated. Previous work that blocked FSH binding to its receptor in mice suggested this hormone may play a part in modulating body weight and energy expenditure after ovariectomy. We used an alternate approach to isolate the individual and combined con...
Source: American Journal of Physiology. Endocrinology and Metabolism - March 27, 2024 Category: Physiology Authors: Andrew E Libby Claudia Solt Matthew Jackman Vanessa Sherk Rebecca M Foright Ginger C Johnson Thi-Tina Nguyen Matthew Breit Nicholas Hulett Michael Rudolph Paul A Roberson Elizabeth Wellberg Purevsuren Jambal Rebecca L Scalzo Janine Higgins T Rajendra Kuma Source Type: research

Impaired muscle oxygenation despite normal pulmonary function in uncomplicated type 2 diabetes
Am J Physiol Endocrinol Metab. 2024 Mar 27. doi: 10.1152/ajpendo.00392.2023. Online ahead of print.ABSTRACTLong-term hyperglycemia in individuals with type 2 diabetes can detrimentally impact pulmonary function and muscle oxygenation. As a result, these factors can impede the body's adaptation to physical exertion. We aimed to evaluate the oxygen pathway during maximal exercise among overweight/obese individuals with uncomplicated type 2 diabetes, in comparison with a group of matched overweight/obese individuals without diabetes, specifically concentrating on the effects on pulmonary function and muscle oxygenation. Fifte...
Source: American Journal of Physiology. Endocrinology and Metabolism - March 27, 2024 Category: Physiology Authors: Islem Jlali Imen Touil Hassen Ibn Hadj Amor Mohamed Amine Bouzid Omar Hammouda Elsa Heyman Pierre Fontaine Hamdi Chtourou Remi Rabasa-Lhoret Georges Baquet Semah Tagougui Source Type: research

Iron metabolism and ferroptosis in Non-alcoholic fatty liver disease: what is our next step?
Am J Physiol Endocrinol Metab. 2024 Mar 20. doi: 10.1152/ajpendo.00260.2023. Online ahead of print.ABSTRACTNon-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disease with increasing prevalence worldwide. NAFLD could develop from simple hepatic steatosis to non-alcoholic steatohepatitis (NASH) causing fibrosis, cirrhosis and even hepatocellular carcinoma. However, the mechanism of NAFLD development was still not be fully defined. Recently, there are emerging evidence showing the dysregulated iron metabolism with elevated serum ferritin and ferroptosis are involved in the NAFLD. The iron metabolism an...
Source: American Journal of Physiology. Endocrinology and Metabolism - March 20, 2024 Category: Physiology Authors: Xiang Shen Ziqi Yu Changli Wei Chong Hu Jianyong Chen Source Type: research

Unveiling cell subpopulations in T1D mouse islets using single-cell RNA sequencing
In this study, we utilized single-cell RNA sequencing (scRNA-seq) to analyze the cellular heterogeneity within the islets of a T1D mouse model. We established a T1D mouse model induced by streptozotocin and identified cell subpopulations using scRNA-seq technology. Our results revealed 11 major cell types in the pancreatic islets of T1D mice, with heterogeneity observed in the alpha and beta cell subgroups, which may play a crucial role in the progression of T1D. Flow cytometry further confirmed a mature alpha and beta cell reduction in T1D mice. Overall, our scRNA-seq analysis provided insights into the cellular heterogen...
Source: American Journal of Physiology. Endocrinology and Metabolism - March 20, 2024 Category: Physiology Authors: Huan Yang Junming Luo Xuyang Liu Yue Luo Xiaoyang Lai Fang Zou Source Type: research

Iron metabolism and ferroptosis in Non-alcoholic fatty liver disease: what is our next step?
Am J Physiol Endocrinol Metab. 2024 Mar 20. doi: 10.1152/ajpendo.00260.2023. Online ahead of print.ABSTRACTNon-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disease with increasing prevalence worldwide. NAFLD could develop from simple hepatic steatosis to non-alcoholic steatohepatitis (NASH) causing fibrosis, cirrhosis and even hepatocellular carcinoma. However, the mechanism of NAFLD development was still not be fully defined. Recently, there are emerging evidence showing the dysregulated iron metabolism with elevated serum ferritin and ferroptosis are involved in the NAFLD. The iron metabolism an...
Source: American Journal of Physiology. Endocrinology and Metabolism - March 20, 2024 Category: Physiology Authors: Xiang Shen Ziqi Yu Changli Wei Chong Hu Jianyong Chen Source Type: research

Unveiling cell subpopulations in T1D mouse islets using single-cell RNA sequencing
In this study, we utilized single-cell RNA sequencing (scRNA-seq) to analyze the cellular heterogeneity within the islets of a T1D mouse model. We established a T1D mouse model induced by streptozotocin and identified cell subpopulations using scRNA-seq technology. Our results revealed 11 major cell types in the pancreatic islets of T1D mice, with heterogeneity observed in the alpha and beta cell subgroups, which may play a crucial role in the progression of T1D. Flow cytometry further confirmed a mature alpha and beta cell reduction in T1D mice. Overall, our scRNA-seq analysis provided insights into the cellular heterogen...
Source: American Journal of Physiology. Endocrinology and Metabolism - March 20, 2024 Category: Physiology Authors: Huan Yang Junming Luo Xuyang Liu Yue Luo Xiaoyang Lai Fang Zou Source Type: research

Iron metabolism and ferroptosis in Non-alcoholic fatty liver disease: what is our next step?
Am J Physiol Endocrinol Metab. 2024 Mar 20. doi: 10.1152/ajpendo.00260.2023. Online ahead of print.ABSTRACTNon-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disease with increasing prevalence worldwide. NAFLD could develop from simple hepatic steatosis to non-alcoholic steatohepatitis (NASH) causing fibrosis, cirrhosis and even hepatocellular carcinoma. However, the mechanism of NAFLD development was still not be fully defined. Recently, there are emerging evidence showing the dysregulated iron metabolism with elevated serum ferritin and ferroptosis are involved in the NAFLD. The iron metabolism an...
Source: American Journal of Physiology. Endocrinology and Metabolism - March 20, 2024 Category: Physiology Authors: Xiang Shen Ziqi Yu Changli Wei Chong Hu Jianyong Chen Source Type: research

Unveiling cell subpopulations in T1D mouse islets using single-cell RNA sequencing
In this study, we utilized single-cell RNA sequencing (scRNA-seq) to analyze the cellular heterogeneity within the islets of a T1D mouse model. We established a T1D mouse model induced by streptozotocin and identified cell subpopulations using scRNA-seq technology. Our results revealed 11 major cell types in the pancreatic islets of T1D mice, with heterogeneity observed in the alpha and beta cell subgroups, which may play a crucial role in the progression of T1D. Flow cytometry further confirmed a mature alpha and beta cell reduction in T1D mice. Overall, our scRNA-seq analysis provided insights into the cellular heterogen...
Source: American Journal of Physiology. Endocrinology and Metabolism - March 20, 2024 Category: Physiology Authors: Huan Yang Junming Luo Xuyang Liu Yue Luo Xiaoyang Lai Fang Zou Source Type: research

Iron metabolism and ferroptosis in Non-alcoholic fatty liver disease: what is our next step?
Am J Physiol Endocrinol Metab. 2024 Mar 20. doi: 10.1152/ajpendo.00260.2023. Online ahead of print.ABSTRACTNon-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disease with increasing prevalence worldwide. NAFLD could develop from simple hepatic steatosis to non-alcoholic steatohepatitis (NASH) causing fibrosis, cirrhosis and even hepatocellular carcinoma. However, the mechanism of NAFLD development was still not be fully defined. Recently, there are emerging evidence showing the dysregulated iron metabolism with elevated serum ferritin and ferroptosis are involved in the NAFLD. The iron metabolism an...
Source: American Journal of Physiology. Endocrinology and Metabolism - March 20, 2024 Category: Physiology Authors: Xiang Shen Ziqi Yu Changli Wei Chong Hu Jianyong Chen Source Type: research

Unveiling cell subpopulations in T1D mouse islets using single-cell RNA sequencing
In this study, we utilized single-cell RNA sequencing (scRNA-seq) to analyze the cellular heterogeneity within the islets of a T1D mouse model. We established a T1D mouse model induced by streptozotocin and identified cell subpopulations using scRNA-seq technology. Our results revealed 11 major cell types in the pancreatic islets of T1D mice, with heterogeneity observed in the alpha and beta cell subgroups, which may play a crucial role in the progression of T1D. Flow cytometry further confirmed a mature alpha and beta cell reduction in T1D mice. Overall, our scRNA-seq analysis provided insights into the cellular heterogen...
Source: American Journal of Physiology. Endocrinology and Metabolism - March 20, 2024 Category: Physiology Authors: Huan Yang Junming Luo Xuyang Liu Yue Luo Xiaoyang Lai Fang Zou Source Type: research

Iron metabolism and ferroptosis in Non-alcoholic fatty liver disease: what is our next step?
Am J Physiol Endocrinol Metab. 2024 Mar 20. doi: 10.1152/ajpendo.00260.2023. Online ahead of print.ABSTRACTNon-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disease with increasing prevalence worldwide. NAFLD could develop from simple hepatic steatosis to non-alcoholic steatohepatitis (NASH) causing fibrosis, cirrhosis and even hepatocellular carcinoma. However, the mechanism of NAFLD development was still not be fully defined. Recently, there are emerging evidence showing the dysregulated iron metabolism with elevated serum ferritin and ferroptosis are involved in the NAFLD. The iron metabolism an...
Source: American Journal of Physiology. Endocrinology and Metabolism - March 20, 2024 Category: Physiology Authors: Xiang Shen Ziqi Yu Changli Wei Chong Hu Jianyong Chen Source Type: research

Unveiling cell subpopulations in T1D mouse islets using single-cell RNA sequencing
In this study, we utilized single-cell RNA sequencing (scRNA-seq) to analyze the cellular heterogeneity within the islets of a T1D mouse model. We established a T1D mouse model induced by streptozotocin and identified cell subpopulations using scRNA-seq technology. Our results revealed 11 major cell types in the pancreatic islets of T1D mice, with heterogeneity observed in the alpha and beta cell subgroups, which may play a crucial role in the progression of T1D. Flow cytometry further confirmed a mature alpha and beta cell reduction in T1D mice. Overall, our scRNA-seq analysis provided insights into the cellular heterogen...
Source: American Journal of Physiology. Endocrinology and Metabolism - March 20, 2024 Category: Physiology Authors: Huan Yang Junming Luo Xuyang Liu Yue Luo Xiaoyang Lai Fang Zou Source Type: research

Iron metabolism and ferroptosis in Non-alcoholic fatty liver disease: what is our next step?
Am J Physiol Endocrinol Metab. 2024 Mar 20. doi: 10.1152/ajpendo.00260.2023. Online ahead of print.ABSTRACTNon-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disease with increasing prevalence worldwide. NAFLD could develop from simple hepatic steatosis to non-alcoholic steatohepatitis (NASH) causing fibrosis, cirrhosis and even hepatocellular carcinoma. However, the mechanism of NAFLD development was still not be fully defined. Recently, there are emerging evidence showing the dysregulated iron metabolism with elevated serum ferritin and ferroptosis are involved in the NAFLD. The iron metabolism an...
Source: American Journal of Physiology. Endocrinology and Metabolism - March 20, 2024 Category: Physiology Authors: Xiang Shen Ziqi Yu Changli Wei Chong Hu Jianyong Chen Source Type: research

Unveiling cell subpopulations in T1D mouse islets using single-cell RNA sequencing
In this study, we utilized single-cell RNA sequencing (scRNA-seq) to analyze the cellular heterogeneity within the islets of a T1D mouse model. We established a T1D mouse model induced by streptozotocin and identified cell subpopulations using scRNA-seq technology. Our results revealed 11 major cell types in the pancreatic islets of T1D mice, with heterogeneity observed in the alpha and beta cell subgroups, which may play a crucial role in the progression of T1D. Flow cytometry further confirmed a mature alpha and beta cell reduction in T1D mice. Overall, our scRNA-seq analysis provided insights into the cellular heterogen...
Source: American Journal of Physiology. Endocrinology and Metabolism - March 20, 2024 Category: Physiology Authors: Huan Yang Junming Luo Xuyang Liu Yue Luo Xiaoyang Lai Fang Zou Source Type: research

The Prostaglandin E < sub > 2 < /sub > EP3 Receptor Has Disparate Effects on Islet Insulin Secretion and Content in β-cells in a High Fat Diet-induced Mouse Model of Obesity
Am J Physiol Endocrinol Metab. 2024 Mar 13. doi: 10.1152/ajpendo.00061.2023. Online ahead of print.ABSTRACTSignaling through Prostaglandin E2 EP3 receptor (EP3) actively contributes to the β-cell dysfunction of type 2 diabetes (T2D). In T2D models, full-body EP3 knockout mice have a significantly worse metabolic phenotype than wild-type controls due to hyperphagia and severe insulin resistance resulting from loss of EP3 in extra-pancreatic tissues, masking any potential beneficial effects of EP3 loss in the β-cell. We hypothesized β-cell-specific EP3 knockout (EP3 βKO) mice would be protected from high-fat diet (HFD)-i...
Source: American Journal of Physiology. Endocrinology and Metabolism - March 13, 2024 Category: Physiology Authors: Joshua C Neuman Austin Reuter Kathryn A Carbajal Michael D Schaid Grant Kelly Kelsey Connors Cecilia Kaiser Joshua Krause Liam D Hurley Angela Olvera Dawn Belt Davis Jaclyn A Wisinski Maureen Gannon Michelle E Kimple Source Type: research