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Molecules, Vol. 28, Pages 6738: A Novel and Efficient Phthalate Hydrolase from Acinetobacter sp. LUNF3: Molecular Cloning, Characterization and Catalytic Mechanism
ou Weixuan Wang Phthalic acid esters (PAEs), which are widespread environmental contaminants, can be efficiently biodegraded, mediated by enzymes such as hydrolases. Despite great advances in the characterization of PAE hydrolases, which are the most important enzymes in the process of PAE degradation, their molecular catalytic mechanism has rarely been systematically investigated. Acinetobacter sp. LUNF3, which was isolated from contaminated soil in this study, demonstrated excellent PAE degradation at 30 &deg;C and pH 5.0&ndash;11.0. After sequencing and annotating the complete genome, the gene dphA...
Source: Molecules - September 21, 2023 Category: Chemistry Authors: Shuanghu Fan Jingjing Guo Shaoyan Han Haina Du Zimeng Wang Yajuan Fu Hui Han Xiaoqiang Hou Weixuan Wang Tags: Article Source Type: research

Magnitude and drug resistance profile of Extended Spectrum β-Lactamase (ESBL) producing gram-negative bacteria from different inanimate objects at Tikur Anbessa Specialized Hospital, Addis Ababa, Ethiopia
Conclusion: It is worrisome to detect ESBL producing gram-negative bacteria from the inanimate objects of TASH, calling for systematic screening of inanimate objects for ESBL and other multidrug-resistant bacteria in the hospital. Furthermore, strengthening the infection prevention practice is vital to halt the transmission of these microorganisms.
Source: Ethiopian Journal of Health Development - September 20, 2023 Category: African Health Authors: Asegedech Asmamaw Jemberu, Kassu Desta Tullu, Yimtubeznash Woldeamanuel Mulate Source Type: research

Biodegradation of fipronil: molecular characterization, degradation kinetics, and metabolites
In this study, fipronil-degrading bacterial species are isolated and characterized from diverse environments using a culture-dependent method followed by 16S rRNA gene sequencing. Phylogenetic analysis showed the homology of organisms with Acinetobacter sp., Streptomyces sp., Pseudomonas sp., Agrobacterium sp., Rhodococcus sp., Kocuria sp., Priestia sp., Bacillus sp., Aeromonas sp., and Pantoea sp. The bacterial degradation potential for fipronil was analyzed through high-performance liquid chromatography (HPLC). Incubation-based degradation studies revealed that Pseudomonas sp. and Rhodococcus sp. were found to be the mos...
Source: Environmental Science and Pollution Research International - September 19, 2023 Category: Environmental Health Authors: Anjali Jaiswal Animesh Tripathi Suresh Kumar Dubey Source Type: research

Prevalence of Antimicrobial Resistance in Gram-Negative Bacteria Bloodstream Infections in Peru and Associated Outcomes: VIRAPERU Study
In conclusion, an important proportion of bloodstream infections in Peru are caused by highly resistant GNB and are associated with high in-hospital mortality.PMID:37722663 | DOI:10.4269/ajtmh.22-0556
Source: The American Journal of Tropical Medicine and Hygiene - September 18, 2023 Category: Tropical Medicine Authors: Fiorella Krapp Coralith Garc ía Noemi Hinostroza Lizeth Astocondor Claudia R Rondon Brecht Ingelbeen Hugo A Alpaca-Salvador Catherine Amaro Carla Aguado Ventura Evelyn Barco-Yaip én Cesar Bocangel Fernandez Alexander Briones Antonio Burgos Rene Campana Source Type: research

Exploring the translational gap of a novel class of Escherichia coli IspE inhibitors
ChemMedChem. 2023 Sep 17:e202300346. doi: 10.1002/cmdc.202300346. Online ahead of print.ABSTRACTDiscovery of novel antibiotics needs multidiscliplinary approaches to gain target enzyme and bacterial activity while aiming for selectivity over mammalian cells. Here we report a multi-parameter optimisation of a fragment-like hit that was identified through a structure-based virtual screening campaign on Escherichia coli IspE crystal structure. Subsequent medicinal chemistry design resulted in a novel class of E. coli IspE inhibitors, exhibiting activity also against the more pathogenic bacteria Pseudomonas aeruginosa and Acin...
Source: ChemMedChem - September 17, 2023 Category: Chemistry Authors: Henni-Karoliina Ropponen Eleonora Diamanti Sandra Johannsen Boris Illarionov Rawia Hamid Miriam Jaki Peter Sass Markus Fischer J örg Haupenthal Anna Katharina Herta Hirsch Source Type: research

Causative Microorganisms Isolated from Patients with Intra-Abdominal Infections and Their Drug Resistance Profiles: An 11-Year (2011-2021) Single-Center Retrospective Study
CONCLUSION: The distribution and antimicrobial susceptibility of the causative microorganisms from patients with IAIs were altered between 2011 and 2021. This finding is valuable for the implementation of evidence-based antimicrobial therapy and provides guidance for the control of hospital infections.PMID:37711085 | DOI:10.3967/bes2023.072
Source: Biomedical and Environmental Sciences : BES - September 15, 2023 Category: Biomedical Science Authors: Rui Ding Rui Rui Ma Ya Li Liu Ying Zhao Li Na Guo Hong Tao Dou Hong Li Sun Wen Jing Liu Li Zhang Yao Wang Ding Ding Li Qiao Lian Yi Ying Chun Xu Source Type: research

Effect of Probiotic Supplementation on the Gut Microbiota Composition of Infants Delivered by Cesarean Section: An Exploratory, Randomized, Open-label, Parallel-controlled Trial
CONCLUSIONS: Our results showed that probiotics supplemented immediately after CS enriched the gut microbiota composition and altered the pattern of early gut colonization.TRIAL REGISTRATION: registration number NCT05086458.PMID:37712964 | DOI:10.1007/s00284-023-03444-4
Source: Current Microbiology - September 15, 2023 Category: Microbiology Authors: Yujiao Gong Hui Zhong Jing Wang Xianggeng Wang Linsheng Huang Yutong Zou HuanLong Qin Rong Yang Source Type: research

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