GSE264016 Porcine Rotavirus VP6 Protein Wrests IPEC-J2 METTL3-Mediated M6A Modification to Fight Antiviral Immune Response
Contributors : Yaxu Liang ; Xuejiao Zhu ; Ning Peng ; Xiang ZhongSeries Type : OtherOrganism : Porcine rotavirus ; Sus scrofaN6-methyladenosine (m6A), the most abundant mRNA modification, can regulate various mRNA metabolism to affect numerous physiological and pathophysiological processes, including immune function. However, the regulation of m6A methylation and its role in immune defense against virus infections remain unexplored. Here, we found that Porcine rotavirus (PoRV) infection decreased global m6A levels in host cells by downregulating m6A writer METTL3. Remarkably, knockdown of Mettl3 or m6A reader Ythdf2 en...
Source: GEO: Gene Expression Omnibus - April 19, 2024 Category: Genetics & Stem Cells Tags: Other Porcine rotavirus Sus scrofa Source Type: research
Viruses, Vol. 16, Pages 623: ML241 Antagonizes ERK 1/2 Activation and Inhibits Rotavirus Proliferation
In this study, a new anti-rotavirus compound was selected by ELISA, and cell activity was detected from 453 small-molecule compounds. The anti-RV effects and underlying mechanisms of the screened compounds were explored. In vitro experimental results showed that the small-molecule compound ML241 has a good effect on inhibiting rotavirus proliferation and has low cytotoxicity during the virus adsorption, cell entry, and replication stages. In addition to its in vitro effects, ML241 also exerted anti-RV effects in a suckling mouse model. Transcriptome sequencing was performed after adding ML241 to cells infected with RV. The...
Source: Viruses - April 17, 2024 Category: Virology Authors: Jinlan Wang Xiaoqing Hu Jinyuan Wu Xiaochen Lin Rong Chen Chenxing Lu Xiaopeng Song Qingmei Leng Yan Li Xiangjing Kuang Jinmei Li Lida Yao Xianqiong Tang Jun Ye Guangming Zhang Maosheng Sun Yan Zhou Hongjun Li Tags: Article Source Type: research
Effect of Non-Rotavirus Enteric Infections on Vaccine Efficacy in a ROTASIIL Clinical Trial
This study examined the relative proportion of enteric pathogens associated with severe gastroenteritis (GE) among children younger than 2 years in a phase III efficacy trial of the ROTASIIL® vaccine in India, evaluated the impact of co-infections on vaccine efficacy (VE), and characterized the association between specific pathogens and the clinical profile of severe GE. Stored stool samples collected from cases of severe GE in the phase III trial were tested by quantitative polymerase chain reaction using TaqMan™ Array Cards. Etiology was attributed by calculating the adjusted attributable fraction (AF) for each pathog...
Source: The American Journal of Tropical Medicine and Hygiene - April 16, 2024 Category: Tropical Medicine Authors: Dilip Abraham Prasanna Samuel Premkumar James A Platts-Mills Tushar Tewari Niranjan Bhat Revathi Rajendiran Hemavathi Gunalan Gagandeep Kang Source Type: research
Effect of Non-Rotavirus Enteric Infections on Vaccine Efficacy in a ROTASIIL Clinical Trial
This study examined the relative proportion of enteric pathogens associated with severe gastroenteritis (GE) among children younger than 2 years in a phase III efficacy trial of the ROTASIIL® vaccine in India, evaluated the impact of co-infections on vaccine efficacy (VE), and characterized the association between specific pathogens and the clinical profile of severe GE. Stored stool samples collected from cases of severe GE in the phase III trial were tested by quantitative polymerase chain reaction using TaqMan™ Array Cards. Etiology was attributed by calculating the adjusted attributable fraction (AF) for each pathog...
Source: Am J Trop Med Hyg - April 16, 2024 Category: Infectious Diseases Authors: Dilip Abraham Prasanna Samuel Premkumar James A Platts-Mills Tushar Tewari Niranjan Bhat Revathi Rajendiran Hemavathi Gunalan Gagandeep Kang Source Type: research
Effect of Non-Rotavirus Enteric Infections on Vaccine Efficacy in a ROTASIIL Clinical Trial
This study examined the relative proportion of enteric pathogens associated with severe gastroenteritis (GE) among children younger than 2 years in a phase III efficacy trial of the ROTASIIL® vaccine in India, evaluated the impact of co-infections on vaccine efficacy (VE), and characterized the association between specific pathogens and the clinical profile of severe GE. Stored stool samples collected from cases of severe GE in the phase III trial were tested by quantitative polymerase chain reaction using TaqMan™ Array Cards. Etiology was attributed by calculating the adjusted attributable fraction (AF) for each pathog...
Source: The American Journal of Tropical Medicine and Hygiene - April 16, 2024 Category: Tropical Medicine Authors: Dilip Abraham Prasanna Samuel Premkumar James A Platts-Mills Tushar Tewari Niranjan Bhat Revathi Rajendiran Hemavathi Gunalan Gagandeep Kang Source Type: research
Etiologie des gastroent érites du nouveau-né au centre hospitalier d'Amiens
Med Mal Infect. 1982 Jul;12(7):424-427. doi: 10.1016/S0399-077X(82)80009-7. Epub 2005 May 26.ABSTRACTAn epidemiological study has been carried on at the Amiens' Gynecology-Obstetrical center with the aim to determinate the aetiology of acute gastrointestinal disorders who affected part of the newborns over a two months period. Rotavirus was found in 16 babies (33 %) by electronmicroscopy and Enzyme-linked-Immuno-sorbent-Assay (ELISA). In one case, a « small round viruswas associated with the Rotavirus. Enteropathogenic bacteria were isolated only two times in this syndrom. The infection seem to releave of nosocomial conta...
Source: Medecine et Maladies Infectieuses - April 15, 2024 Category: Infectious Diseases Authors: F Lema A Rosetto M Driencourt P Verhoest M Vitse Ph Daniel Source Type: research
Viral gastroenteritis
Med Mal Infect. 1987 Nov;17(11):618-625. doi: 10.1016/S0399-077X(87)80114-2. Epub 2005 Jun 2.ABSTRACTMany virus are able to induce gastroenteritis, especially in young children with immunological or nutritional defects. The most frequently observed, using ELISA, are Rotavirus. The others : Norwalk agent, Coronavirus, Breda virus, are identified using electron microscope. Using symptomatic therapy, prognosis is related to precocious hydratation.PMID:38620189 | PMC:PMC7131556 | DOI:10.1016/S0399-077X(87)80114-2 (Source: Medecine et Maladies Infectieuses)
Source: Medecine et Maladies Infectieuses - April 15, 2024 Category: Infectious Diseases Authors: F Lamouliatte P Du Pasquier Source Type: research
Etude épidémiologique de gastroentérites infantiles d'origine virale dans la région amienoise
Med Mal Infect. 1981 Nov;11(11):576-580. doi: 10.1016/S0399-077X(81)80043-1. Epub 2005 May 26.ABSTRACTA systematic survey was carried on stools from 130 children suffering of acute gastroenteritis. Electron microscopy, enzymo-linked immunosorbent assay (ELISA) and counter electrophoresis were employed. This survey allowed to the detection by electron microscopy of Rotavirus (40 cases), Coronaviruses (3 cases), Astroviruses (2 cases), Adenoviruses (2 cases) and Small Round Viruses (1 case). Serological tests (complement fixation, ELISA and counter electrophoresis) done with 86 sera showed a good correlation with results obt...
Source: Medecine et Maladies Infectieuses - April 15, 2024 Category: Infectious Diseases Authors: F Lema A Roseto G Duverlie J Peries Ph Daniel Source Type: research
Etiology of infantile acute gastro-enteritis in usual practice in hospital
Med Mal Infect. 1984 Jun;14(6):342-346. doi: 10.1016/S0399-077X(84)80070-0. Epub 2005 May 26.ABSTRACTThe authors report the results of a study of enteric disease etiology from chilfren during one year. These study is about 155 diarrheas, 16 from them content leukocytes. Rotavirus are discovered in 38,1 % diarrheas without leukocytes. Between the bacteria isolated, Salmonellaare the most frequently enteric bacterias. After, Campylobacter, E. colientero pathogenic. The isolation of Yersinia enterocoliticaand Shigellaare frequentless. A particular mention about the associations : rotavirus - bacterias, and about the high freq...
Source: Medecine et Maladies Infectieuses - April 15, 2024 Category: Infectious Diseases Authors: J P Hebert R Caillet Source Type: research
Lactiplantibacillus plantarum surface-displayed VP6 (PoRV) protein can prevent PoRV infection in piglets
Int Immunopharmacol. 2024 Apr 13;133:112079. doi: 10.1016/j.intimp.2024.112079. Online ahead of print.ABSTRACTPorcine rotavirus (PoRV) poses a threat to the development of animal husbandry and human health, leading to substantial economic losses. VP6 protein is the most abundant component in virus particles and also the core structural protein of the virus. Firstly, this study developed an antibiotic-resistance-free, environmentally friendly expression vector, named asd-araC-PBAD-alr (AAPA). Then Recombinant Lactiplantibacillus plantarum (L. plantarum) strains induced by arabinose to express VP6 and VP6-pFc fusion proteins...
Source: International Immunopharmacology - April 14, 2024 Category: Allergy & Immunology Authors: Junhong Wang Haixu Wang Dongliang Zhang Fangyuan Liu Xiaoxu Li Ming Gao Mingyang Cheng Hongyu Bao Jiaxing Zhan Yan Zeng Chunfeng Wang Xin Cao Source Type: research
Lactiplantibacillus plantarum surface-displayed VP6 (PoRV) protein can prevent PoRV infection in piglets
Int Immunopharmacol. 2024 Apr 13;133:112079. doi: 10.1016/j.intimp.2024.112079. Online ahead of print.ABSTRACTPorcine rotavirus (PoRV) poses a threat to the development of animal husbandry and human health, leading to substantial economic losses. VP6 protein is the most abundant component in virus particles and also the core structural protein of the virus. Firstly, this study developed an antibiotic-resistance-free, environmentally friendly expression vector, named asd-araC-PBAD-alr (AAPA). Then Recombinant Lactiplantibacillus plantarum (L. plantarum) strains induced by arabinose to express VP6 and VP6-pFc fusion proteins...
Source: International Immunopharmacology - April 14, 2024 Category: Allergy & Immunology Authors: Junhong Wang Haixu Wang Dongliang Zhang Fangyuan Liu Xiaoxu Li Ming Gao Mingyang Cheng Hongyu Bao Jiaxing Zhan Yan Zeng Chunfeng Wang Xin Cao Source Type: research
Etiology of diarrheal hospitalizations following rotavirus vaccine implementation and association of enteric pathogens with malnutrition among under-five children in India
AbstractMalnourished children are at higher risk of mortality and morbidity following diarrheal illness and certain enteropathogens have been associated with malnutrition in children. Very few studies have comprehensively looked at the etiology of diarrhea in malnourished children and most have used conventional diagnostic methods with suboptimal sensitivity. We used a highly sensitive molecular approach against a broad range of pathogens causing diarrhea and examined their association with malnutrition. In addition, we looked at the pathogen diversity of pediatric diarrhea, three years after the nationwide rotavirus vacci...
Source: Gut Pathogens - April 10, 2024 Category: Microbiology Source Type: research
Spectroscopic Analysis of the Bacterially Expressed Head Domain of Rotavirus VP6
Biosci Rep. 2024 Apr 9:BSR20232178. doi: 10.1042/BSR20232178. Online ahead of print.ABSTRACTThe rotavirus capsid protein VP6 forms the middle of three protein layers and is responsible for many critical steps in the viral life cycle. VP6 as a structural protein can be used in various applications including as a subunit vaccine component. The head domain of VP6 (VP6H) contains key sequences that allow the protein to trimerize and that represent epitopes that are recognized by human antibodies in the viral particle. The domain is rich in beta-sheet secondary structures. Here VP6H was solubilised from bacterial inclusion bodi...
Source: Bioscience Reports - April 9, 2024 Category: Biomedical Science Authors: Milaan Simone Strachan Tshepo Mashapa Samantha Gildenhuys Source Type: research
Spectroscopic Analysis of the Bacterially Expressed Head Domain of Rotavirus VP6
Biosci Rep. 2024 Apr 9:BSR20232178. doi: 10.1042/BSR20232178. Online ahead of print.ABSTRACTThe rotavirus capsid protein VP6 forms the middle of three protein layers and is responsible for many critical steps in the viral life cycle. VP6 as a structural protein can be used in various applications including as a subunit vaccine component. The head domain of VP6 (VP6H) contains key sequences that allow the protein to trimerize and that represent epitopes that are recognized by human antibodies in the viral particle. The domain is rich in beta-sheet secondary structures. Here VP6H was solubilised from bacterial inclusion bodi...
Source: Bioscience Reports - April 9, 2024 Category: Biomedical Science Authors: Milaan Simone Strachan Tshepo Mashapa Samantha Gildenhuys Source Type: research
Spectroscopic Analysis of the Bacterially Expressed Head Domain of Rotavirus VP6
Biosci Rep. 2024 Apr 9:BSR20232178. doi: 10.1042/BSR20232178. Online ahead of print.ABSTRACTThe rotavirus capsid protein VP6 forms the middle of three protein layers and is responsible for many critical steps in the viral life cycle. VP6 as a structural protein can be used in various applications including as a subunit vaccine component. The head domain of VP6 (VP6H) contains key sequences that allow the protein to trimerize and that represent epitopes that are recognized by human antibodies in the viral particle. The domain is rich in beta-sheet secondary structures. Here VP6H was solubilised from bacterial inclusion bodi...
Source: Bioscience Reports - April 9, 2024 Category: Biomedical Science Authors: Milaan Simone Strachan Tshepo Mashapa Samantha Gildenhuys Source Type: research
