Overexpression of Rhodiola crenulata glutathione peroxidase 5 increases cold tolerance and enhances the pharmaceutical value of the hairy roots
In this study, we investigated the role of GPX5 in Rhodiola crenulata's cold tolerance mechanisms. By overexpressing Rhodiola crenulata GPX5 (RcGPX5) in yeast and Arabidopsis thaliana, we observed down-regulation of Arabidopsis thaliana GPX5 (AtGPX5) and increased cold tolerance in both organisms. Furthermore, the levels of antioxidants and enzyme activities in the ascorbate-glutathione cycle were elevated, and cold-responsive genes such as AtCBFs and AtCORs were induced. Additionally, RcGPX5 overexpressing lines showed insensitivity to exogenous abscisic acid (ABA), suggesting a negative regulation of the ABA pathway by R...
Source: Gene - April 14, 2024 Category: Genetics & Stem Cells Authors: Kai Hou Lu Cao Wen Li Zhi-Hui Fang Daqiang Sun Zhigang Guo Lipeng Zhang Source Type: research
Discovery of a botanical compound as a broad-spectrum inhibitor against gut microbial β-glucuronidases from the Tibetan medicine Rhodiola crenulata
Int J Biol Macromol. 2024 Mar 29:131150. doi: 10.1016/j.ijbiomac.2024.131150. Online ahead of print.ABSTRACTGut microbial β-glucuronidases (gmβ-GUS) played crucial roles in regulating a variety of endogenous substances and xenobiotics on the circulating level, thus had been recognized as key modulators of drug toxicity and human diseases. Inhibition or inactivation of gmβ-GUS enzymes has become a promising therapeutic strategy to alleviate drug-induced intestinal toxicity. Herein, the Rhodiola crenulata extract (RCE) was found with potent and broad-spectrum inhibition on multiple gmβ-GUS enzymes. Subsequently, the anti...
Source: International Journal of Biological Macromolecules - March 31, 2024 Category: Biochemistry Authors: Lu Chen Xu-Dong Hou Guang-Hao Zhu Jian Huang Zhao-Bin Guo Ya-Ni Zhang Jian-Ming Sun Li-Juan Ma Shou-De Zhang Jie Hou Guang-Bo Ge Source Type: research
Discovery of a botanical compound as a broad-spectrum inhibitor against gut microbial β-glucuronidases from the Tibetan medicine Rhodiola crenulata
Int J Biol Macromol. 2024 Mar 29:131150. doi: 10.1016/j.ijbiomac.2024.131150. Online ahead of print.ABSTRACTGut microbial β-glucuronidases (gmβ-GUS) played crucial roles in regulating a variety of endogenous substances and xenobiotics on the circulating level, thus had been recognized as key modulators of drug toxicity and human diseases. Inhibition or inactivation of gmβ-GUS enzymes has become a promising therapeutic strategy to alleviate drug-induced intestinal toxicity. Herein, the Rhodiola crenulata extract (RCE) was found with potent and broad-spectrum inhibition on multiple gmβ-GUS enzymes. Subsequently, the anti...
Source: International Journal of Biological Macromolecules - March 31, 2024 Category: Biochemistry Authors: Lu Chen Xu-Dong Hou Guang-Hao Zhu Jian Huang Zhao-Bin Guo Ya-Ni Zhang Jian-Ming Sun Li-Juan Ma Shou-De Zhang Jie Hou Guang-Bo Ge Source Type: research
Neuromodulatory effects induced by the association of Moringa oleifera Lam., Tribulus terrestris L., Undaria pinnatidifida extracts in the hypothalamus
Chem Biodivers. 2024 Mar 25:e202302075. doi: 10.1002/cbdv.202302075. Online ahead of print.ABSTRACTThe present study investigated the role of a commercial formulation constituted by herbal extracts from Rhodiola rosea, Undaria pinnatifida, Tribulus terrestris, and Moringa oleifera. The formulation was analysed for determining the content in total phenols and flavonoids and scavenging/reducing properties. The formulation was also tested on isolated mouse hypothalamus in order to investigate effects on serotonin, dopamine, neuropeptide Y (NPY), and orexin A. The gene expression of gonadrotopin releasing hormone (GnRH) was al...
Source: Chemistry and Biodiversity - March 25, 2024 Category: Biochemistry Authors: Annalisa Chiavaroli Simonetta Cristina Di Simone Alessandra Acquaviva Nilofar Nilofar Maria Loreta Libero Luigi Brunetti Lucia Recinella Sheila Leone Giustino Orlando Gokhan Zengin Maura Di Vito Luigi Menghini Claudio Ferrante Source Type: research
Neuromodulatory effects induced by the association of Moringa oleifera Lam., Tribulus terrestris L., Undaria pinnatidifida extracts in the hypothalamus
Chem Biodivers. 2024 Mar 25:e202302075. doi: 10.1002/cbdv.202302075. Online ahead of print.ABSTRACTThe present study investigated the role of a commercial formulation constituted by herbal extracts from Rhodiola rosea, Undaria pinnatifida, Tribulus terrestris, and Moringa oleifera. The formulation was analysed for determining the content in total phenols and flavonoids and scavenging/reducing properties. The formulation was also tested on isolated mouse hypothalamus in order to investigate effects on serotonin, dopamine, neuropeptide Y (NPY), and orexin A. The gene expression of gonadrotopin releasing hormone (GnRH) was al...
Source: Chemistry and Biodiversity - March 25, 2024 Category: Biochemistry Authors: Annalisa Chiavaroli Simonetta Cristina Di Simone Alessandra Acquaviva Nilofar Nilofar Maria Loreta Libero Luigi Brunetti Lucia Recinella Sheila Leone Giustino Orlando Gokhan Zengin Maura Di Vito Luigi Menghini Claudio Ferrante Source Type: research
Investigating the multitarget pharmacological mechanism of < em > Rhodiola wallichiana < /em > var. < em > cholaensis < /em > acting on angina pectoris using combined network pharmacology and molecular docking
CONCLUSIONS: The results of this study preliminarily identify the potential targets and signaling pathways of RW in AP therapy and lay a promising foundation for further experimental studies and clinical trials.PMID:38505080 | PMC:PMC10944760 | DOI:10.21037/jtd-23-1891 (Source: Journal of Thoracic Disease)
Source: Journal of Thoracic Disease - March 20, 2024 Category: Respiratory Medicine Authors: Haitao Zhang Xudong Zhuang Zhixiong Li Xinrui Wang Source Type: research