Formulation and Optimization of Mitochondria-Targeted Polymeric Nanoparticles
Targeted delivery of therapeutics to the mitochondria of cells without alteration of drug properties can be a vital technique in the treatment of a variety of mitochondrial-dysfunction-related diseases. Herein, we describe a detailed protocol for synthesis and characterization of a functionalized polymer to build mitochondria-targeted nanoparticles (NPs). The block polymer was decorated with a lipophilic triphenylphosphonium (TPP) cation for mitochondrial trafficking of payload-loaded polymeric NPs. TPP-based lipophilic cations have the ability to cross the mitochondrial membrane. A mitochondria-targeted block copolymer po...
Source: Springer protocols feed by Pharmacology/Toxicology - February 5, 2015 Category: Drugs & Pharmacology Source Type: news
Whole bowel irrigation? Don’t just do something, stand there!
3.5 out of 5 stars
Position paper update: Whole bowel irrigation for gastrointestinal decontamination of overdose patients. Thanacoody R et al. Clin Toxicol 2015 Jan;53:5-12.
Abstract
In 1997 the American Academy of Clinical Toxicology (AACT) and the Euroopean Association of Poisons Centre and Clinical Toxicologists (EAPCCT) published a position paper regarding the use of whole bowel irrigation (WBI) for gastrointestinal decontamination in the poisoned patient. The key conclusions were that no evidence existed showing that WBI improved clinical outcome, and that it should not be used routinely but “may be considered...
Source: The Poison Review - January 13, 2015 Category: Toxicology Authors: Leon Tags: Medical gastrointestinal decontamination overdose poisoning polyethylene glycol position paper whole bowel irrigation Source Type: news
FDA Questioning Miralax Use in Kids (FREE)
By Kelly Young
Edited by David G. Fairchild, MD, MPH, and Lorenzo Di Francesco, MD, FACP, FHM
At the request of the FDA, scientists are studying how children metabolize the active ingredient in the adult laxative polyethylene glycol … (Source: Physician's First Watch current issue)
Source: Physician's First Watch current issue - January 7, 2015 Category: Primary Care Source Type: news
Polyethylene Glycol (PEG) and Electrolytes
Title: Polyethylene Glycol (PEG) and ElectrolytesCategory: MedicationsCreated: 3/2/2005 12:00:00 AMLast Editorial Review: 11/19/2014 12:00:00 AM (Source: MedicineNet Digestion General)
Source: MedicineNet Digestion General - November 19, 2014 Category: Nutrition Source Type: news
A phase change storage material that may be used in the fire resistance of building structure - Wang P, Li N, Zhao C, Wu L, Han G.
This study prepared polyethylene glycol/silicon dioxide composite, a kind of form-stable phase change material. The composites can be made into mortar which is able to adhere to the surface of building structure and absorb the fire heat. This paper aims to... (Source: SafetyLit: All (Unduplicated))
Source: SafetyLit: All (Unduplicated) - November 15, 2014 Category: Global & Universal Tags: Burns, Electricity, Explosions, Fire, Scalds Source Type: news
Hydrolytically Degradable Polyethylene Glycol (PEG) Hydrogel: Synthesis, Gel Formation, and Characterization
Degradable tight-mesh hydrogel scaffolds are a promising cell carrier for neural transplantation because they can match the stiffness of the native tissue, decrease immunogenicity of the transplant, and degrade over time as the new tissue forms. Here we describe the synthesis and gel formation of a hydrolytically degradable polyethylene glycol (PEG) hydrogel, where the mild gelation conditions allow for cell encapsulation prior to gel formation. We also describe how to measure swelling ratio and mass loss of the hydrogel over time in order to assess hydrogel degradation. (Source: Springer protocols feed by Neuroscience)
Source: Springer protocols feed by Neuroscience - November 4, 2014 Category: Neuroscience Source Type: news
Mircera (Methoxy Polyethylene glycol-epoetin beta) - updated on RxList
(Source: RxList - New and Updated Drug Monographs)
Source: RxList - New and Updated Drug Monographs - October 16, 2014 Category: Drugs & Pharmacology Source Type: news
polyethylene glycol 3350 (Miralax, GoLytely)
Title: polyethylene glycol 3350 (Miralax, GoLytely)Category: MedicationsCreated: 3/2/2005 12:00:00 AMLast Editorial Review: 10/2/2014 12:00:00 AM (Source: MedicineNet Crohn's Disease General)
Source: MedicineNet Crohn's Disease General - October 2, 2014 Category: Gastroenterology Source Type: news
polyethylene glycol 3350 (Miralax, GoLytely)
Title: polyethylene glycol 3350 (Miralax, GoLytely)Category: MedicationsCreated: 3/2/2005 12:00:00 AMLast Editorial Review: 10/2/2014 12:00:00 AM (Source: MedicineNet Medications General)
Source: MedicineNet Medications General - October 2, 2014 Category: Drugs & Pharmacology Source Type: news
What Causes Abdominal Distention?
Discussion
Constipation generally is defined as infrequent or painful defecation. It often is due to passing large hard stool infrequently which causes painful defecation and then withholding behaviors. As stool withholding continues, the rectum dilates and gradually accommodates with the normal defecation urge disappearing. Chronic rectal distension results in both loss of rectal sensitivity, and loss of urge to defecate, which can lead to encopresis. Abdominal distention because of stool retention occurs frequently. Treatment includes colonic evacuation, establishing regular bowel habits, eating a balanced diet with diet...
Source: PediatricEducation.org - July 21, 2014 Category: Pediatrics Authors: pediatriceducationmin Tags: Uncategorized Source Type: news
Proteomic Approaches to Identify Cold-Regulated Plasma Membrane Proteins
Plasma membrane is the primary determinant of freezing tolerance in plants because of its central role in freeze–thaw cycle. Changes in the plasma membrane proteins have been one of the major research areas in plant cold acclimation. To obtain comprehensive profiles of the plasma membrane proteomes and their changes during the cold acclimation process, a plasma membrane purification method using a dextran–polyethylene glycol two polymer system and a mass spectrometry-based shotgun proteomics method using nano-LC-MS/MS for the plasma membrane proteins are described. The proteomic results obtained are further app...
Source: Springer protocols feed by Plant Sciences - May 27, 2014 Category: Biology Source Type: news
Good quick review of bupropion toxicity, but . . . whole bowel irrigation? Seriously??
2.5 out of 5 stars
Bupropion Abuse and Overdose. Stall N et al. CMAJ 2014 Apr 28 [Epub ahead of print]
No abstract available online
This one-page précis is part of CMAJ‘s “Five Things To Know About _______” series. The key points include:
Bupropion blocks reuptake of dopamine and norepinephrine, and also blocks the action of acetylcholine at nicotinic receptors.
Burpropion is abused recreationally for its mild cocaine-like “high.”
Routes of bupropion abuse include snorting, ingestion, and intravenous injection.
Brupropion lowers the threshold for seizures, which may be delayed as long as ...
Source: The Poison Review - May 9, 2014 Category: Toxicology Authors: Leon Tags: Medical abuse bupropion overdose seizures Source Type: news
Plastid Transformation in Lettuce (Lactuca sativa L.) by Polyethylene Glycol Treatment of Protoplasts
A detailed protocol for PEG-mediated plastid transformation of Lactuca sativa cv. Flora, using leaf protoplasts, is described. Successful plastid transformation using protoplasts requires a large number of viable cells, high plating densities, and an efficient regeneration system. Transformation was achieved using a vector that targets genes to the trnI/trnA intergenic region of the lettuce plastid genome. The aadA gene, encoding an adenylyltransferase enzyme that confers spectinomycin resistance, was used as a selectable marker. With the current method, the expected transformation frequency is 1–2 spectinomycin-resi...
Source: Springer protocols feed by Plant Sciences - March 6, 2014 Category: Biology Source Type: news
Nicotiana tabacum : PEG-Mediated Plastid Transformation
Stable plastid transformation in Nicotiana tabacum has been achieved by using two different methods, the biolistic method, using a particle gun, and the polyethylene glycol (PEG)-mediated transformation. PEG-mediated plastid transformation involves the treatment of isolated protoplasts (plant cells without cell wall) with PEG in the presence of DNA. We have previously shown that in Nicotiana tabacum both methods are equally efficient. The PEG-mediated transformation efficiencies range between 20 and 50 plastid transformants per experiment (106 viable treated protoplasts). One advantage of the PEG method is that no expensiv...
Source: Springer protocols feed by Plant Sciences - March 6, 2014 Category: Biology Source Type: news
Consumer Information on: ReSure® Sealant - P130004
ReSure Sealant prevents leakage from a cut (incision) in the cornea. It is made of two solutions, a polyethylene glycol solution and a trilysine amine solution. It is applied to a corneal incision using an applicator. (Source: Food and Drug Adminstration (FDA): CDRHNew)
Source: Food and Drug Adminstration (FDA): CDRHNew - January 15, 2014 Category: Medical Equipment Source Type: news
