A Partially Flipped Physiology Classroom Improves the Deep Learning Approach of Medical Students
This study aimed to compare the impact of the partially flipped physiology classroom (PFC) and the traditional lecture-based classroom (TLC) on students' learning approaches. The study was conducted over five months at Xiangya School of Medicine from February to July 2022 and comprised 71 students majoring in clinical medicine. The experimental group (n = 32) received PFC teaching, while the control group (n = 39) received TLC. The Revised Two-Factor Study Process Questionnaire (R-SPQ-2F) was used to assess the impact of different teaching methods on students' learning approaches. After the PFC, students got significantly ...
Source: Adv Physiol Educ - April 11, 2024 Category: Universities & Medical Training Authors: Ziqi Liu Yangting Xu Yicheng Lin Pei Yu Ming Ji Ziqiang Luo Source Type: research
The Inverted Case Study Technique: Changing the Traditional Case-Study Approach to Prioritize Physiological Mechanisms Over Correct Diagnosis and Treatment
Adv Physiol Educ. 2024 Apr 11. doi: 10.1152/advan.00180.2023. Online ahead of print.ABSTRACTThe traditional case study has been used as a learning tool for the past 100 years and in our program, graduate physiology students are presented with a real-world scenario and must determine the diagnosis and treatment of the patient. We found that students defaulted to memorization of disease with treatment and bypassed gaining an understanding of the mechanistic physiology behind disease and treatment. To adjust our student's approach, we developed a novel way to enhance student learning. In order to accomplish this shift from me...
Source: Adv Physiol Educ - April 11, 2024 Category: Universities & Medical Training Authors: Robert E Brainard Daniela G L Terson de Paleville Michael C Long Daniel G Hughes Lewis J Watson Gary Anderson Source Type: research
Nascent shifts in renal cellular metabolism, structure, and function due to chronic empagliflozin in prediabetic mice
Am J Physiol Cell Physiol. 2024 Apr 1;326(4):C1272-C1290. doi: 10.1152/ajpcell.00446.2023. Epub 2024 Mar 4.ABSTRACTSodium-glucose cotransporter, type 2 inhibitors (SGLT2i) are emerging as the gold standard for treatment of type 2 diabetes (T2D) with renal protective benefits independent of glucose lowering. We took a high-level approach to evaluate the effects of the SGLT2i, empagliflozin (EMPA) on renal metabolism and function in a prediabetic model of metabolic syndrome. Male and female 12-wk-old TallyHo (TH) mice, and their closest genetic lean strain (Swiss-Webster, SW) were treated with a high-milk-fat diet (HMFD) plu...
Source: American Journal of Physiology. Cell Physiology - April 11, 2024 Category: Cytology Authors: Blythe D Shepard Jennifer Chau Ryan Kurtz Avi Z Rosenberg Pinaki Sarder Samuel P Border Brandon Ginley Olga Rodriguez Chris Albanese Grace Knoer Aarenee Greene Aline M A De Souza Suman Ranjit Moshe Levi Carolyn M Ecelbarger Source Type: research
Esm-1 mediates transcriptional polarization associated with diabetic kidney disease
Am J Physiol Renal Physiol. 2024 Apr 11. doi: 10.1152/ajprenal.00419.2023. Online ahead of print.ABSTRACTEsm-1, endothelial cell-specific molecule-1, is a susceptibility gene for diabetic kidney disease (DKD) and is a secreted proteoglycan, with notable expression in kidney, which attenuates inflammation and albuminuria. However, little is known about Esm1 expression in mature tissues in the presence or absence of diabetes. We utilized publicly available single-cell RNA sequencing data to characterize Esm1 expression in 27,786 renal endothelial cells (RECs) obtained from four human and three mouse databases. We validated o...
Source: American Journal of Physiology. Renal Physiology - April 11, 2024 Category: Physiology Authors: Alexandre Gaudet Xiaoyi Zheng Neeraja Kambham Vivek Bhalla Source Type: research
Long-term follow-up of TREK-1 KO mice reveals the development of bladder hypertrophy and impaired bladder smooth muscle contractility with age
This study aimed to characterize the long-term effects of TREK-1 on bladder function by using global and smooth muscle specific TREK-1 knock out (KO) mice. Bladder morphology, bladder smooth muscle (BSM) contractility, and voiding patterns were evaluated up to 12 months of age. Both sexes were included in this study to probe the potential sex differences. Smooth muscle specific TREK-1 KO mice were used to distinguish the effects of TREK-1 downregulation in BSM from the neural pathways involved in the control of bladder contraction and relaxation. TREK-1 KO mice developed enlarged urinary bladders (by 60.0% for males and by...
Source: American Journal of Physiology. Renal Physiology - April 11, 2024 Category: Physiology Authors: Alison Xiaoqiao Xie Nao Iguchi Anna P Malykhina Source Type: research
Mechanisms and Implications of Podocyte Autophagy in Chronic Kidney Disease
Am J Physiol Renal Physiol. 2024 Apr 11. doi: 10.1152/ajprenal.00415.2023. Online ahead of print.ABSTRACTAutophagy is a protective mechanism through which cells degrade and recycle proteins and organelles to maintain cellular homeostasis and integrity. An accumulating body of evidence underscores the significant impact of dysregulated autophagy on podocyte injury in chronic kidney disease. In this review, we provide a comprehensive overview of the diverse types of autophagy and their regulation in cellular homeostasis, with a specific emphasis on podocytes. Furthermore, we discuss recent findings that focus on the function...
Source: American Journal of Physiology. Renal Physiology - April 11, 2024 Category: Physiology Authors: Rachel Njeim Sandra Merscher Alessia Fornoni Source Type: research
Growth performance, health status, gut microbiome, and expression of immune and growth-related genes of rainbow trout (Oncorhynchus mykiss) fed diets with pea protein replacement of fish meal
Comp Biochem Physiol B Biochem Mol Biol. 2024 Apr 9;273:110968. doi: 10.1016/j.cbpb.2024.110968. Online ahead of print.ABSTRACTThe need for fish meal constrains fish farming and significantly impacts sustainability of the aquaculture industry. Hence, it is important to investigate the use of plant-based protein sources in fish diets. The present study was conducted to determine the effects of different levels of fish meal (FM) replacement by pea protein (PP) in a 60-day feeding experiment in rainbow trout, Oncorhynchus mykiss. Effects on growth performance, body composition, hematology, serum biochemistry and immunology, a...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - April 11, 2024 Category: Biochemistry Authors: Osman Sabri Kesbi ç Ümit Acar Fevziye I şıl Kesbiç Sevdan Y ılmaz Source Type: research
Maximum storage time of refrigerated blood and frozen plasma samples from tambaqui, Colossoma macropomum
This study sought to determine the maximum time refrigerated blood and frozen plasma samples of the tambaqui, Colossoma macropomum, can be stored without affecting analysis. Samples from 12 fish were obtained, stored under refrigeration at 4 °C and evaluated after 0, 24, 48, 72, and 96 h, while samples from 14 fish were centrifuged, and the resulting plasma was frozen at -20 °C and then evaluated after 0, 8, 12, 16 and 20 weeks. The parameters analyzed were hematocrit (Ht), hemoglobin content (Hb), total erythrocytes (RBC), total (WBC) and differential leukocytes, total thrombocytes (TC), glucose content (Glc), total pro...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - April 11, 2024 Category: Biochemistry Authors: Marcela C Sena F ábio C Sterzelecki Paola Fabiana F Gomes Helen Cristiane A Souza Nuno Filipe A Correia de Melo J Natividade B Baldisserotto Glauber David A Palheta Simone do Socorro D Santos Source Type: research
Molecular characterization of transcription factor CREB3L2 and CREB3L3 and their role in melanogenesis in Pacific oysters (Crassostrea gigas)
In this study, we characterized two CREB genes (CREB3L2 and CREB3L3) from Pacific oysters. Both of them contained a conserved DNA-binding and dimerization domain (a basic-leucine zipper domain). CREB3L2 and CREB3L3 were expressed highly in the mantle tissues and exhibited higher expression levels in the black-shell oyster than in the white. Masson-Fontana melanin staining and immunofluorescence analysis showed that the location of CREB3L2 protein was generally consistent with the distribution of melanin in oyster edge mantle. Dual-luciferase reporter assays revealed that CREB3L2 and CREB3L3 could activate the microphthalmi...
Source: Comparative biochemistry and physiology. Part B, Biochemistry and molecular biology. - April 11, 2024 Category: Biochemistry Authors: Kunyin Jiang Hong Yu Lingfeng Kong Shikai Liu Qi Li Source Type: research
The Inverted Case Study Technique: Changing the Traditional Case-Study Approach to Prioritize Physiological Mechanisms Over Correct Diagnosis and Treatment
Adv Physiol Educ. 2024 Apr 11. doi: 10.1152/advan.00180.2023. Online ahead of print.ABSTRACTThe traditional case study has been used as a learning tool for the past 100 years and in our program, graduate physiology students are presented with a real-world scenario and must determine the diagnosis and treatment of the patient. We found that students defaulted to memorization of disease with treatment and bypassed gaining an understanding of the mechanistic physiology behind disease and treatment. To adjust our student's approach, we developed a novel way to enhance student learning. In order to accomplish this shift from me...
Source: Advances in Physiology Education - April 11, 2024 Category: Physiology Authors: Robert E Brainard Daniela G L Terson de Paleville Michael C Long Daniel G Hughes Lewis J Watson Gary Anderson Source Type: research
37 < sup > th < /sup > Annual Meeting of The Ohio Physiological Society: October 6-7, 2023, The University of Toledo, OH
Adv Physiol Educ. 2024 Apr 11. doi: 10.1152/advan.00234.2023. Online ahead of print.NO ABSTRACTPMID:38602009 | DOI:10.1152/advan.00234.2023 (Source: Advances in Physiology Education)
Source: Advances in Physiology Education - April 11, 2024 Category: Physiology Authors: Charles K Thodeti Source Type: research
Modelling the extracellular potential generated by a muscle fiber as the output signal of a convolutional system
Adv Physiol Educ. 2024 Apr 11. doi: 10.1152/advan.00238.2023. Online ahead of print.ABSTRACTA central topic in Bioelectricity is the generation of the extracellular potential that results from the propagation of a transmembrane action potential along the muscle fiber. However, the way in which the extracellular potential is determined by the propagating action potential is difficult to describe, conceptualize and visualize. Moreover, traditional quantitative approaches aimed at modelling extracellular potentials involve complex mathematical formulations, which do not allow students to visualize how the extracellular potent...
Source: Advances in Physiology Education - April 11, 2024 Category: Physiology Authors: Javier Rodriguez-Falces Source Type: research
A Partially Flipped Physiology Classroom Improves the Deep Learning Approach of Medical Students
This study aimed to compare the impact of the partially flipped physiology classroom (PFC) and the traditional lecture-based classroom (TLC) on students' learning approaches. The study was conducted over five months at Xiangya School of Medicine from February to July 2022 and comprised 71 students majoring in clinical medicine. The experimental group (n = 32) received PFC teaching, while the control group (n = 39) received TLC. The Revised Two-Factor Study Process Questionnaire (R-SPQ-2F) was used to assess the impact of different teaching methods on students' learning approaches. After the PFC, students got significantly ...
Source: Advances in Physiology Education - April 11, 2024 Category: Physiology Authors: Ziqi Liu Yangting Xu Yicheng Lin Pei Yu Ming Ji Ziqiang Luo Source Type: research
Proteomic and metabolomic insights into seed germination of Ferula assa-foetida
J Proteomics. 2024 Apr 9;300:105176. doi: 10.1016/j.jprot.2024.105176. Online ahead of print.ABSTRACTCold stratification is known to affect the speed of seed germination; however, its regulation at the molecular level in Ferula assa-foetida remains ambiguous. Here, we used cold stratification (4 °C in the dark) to induce germination in F. assa-foetida and adopted a proteomic and metabolomic approach to understand the molecular mechanism of germination. Compared to the control, we identified 209 non-redundant proteins and 96 metabolites in germinated F. assa-foetida seed. Results highlight the common and unique regulatory ...
Source: Journal of Proteomics - April 11, 2024 Category: Biochemistry Authors: Ashwani Punia Manglesh Kumari Monika Chouhan Vishal Saini Robin Joshi Ashok Kumar Rajiv Kumar Source Type: research
Nascent shifts in renal cellular metabolism, structure, and function due to chronic empagliflozin in prediabetic mice
Am J Physiol Cell Physiol. 2024 Apr 1;326(4):C1272-C1290. doi: 10.1152/ajpcell.00446.2023. Epub 2024 Mar 4.ABSTRACTSodium-glucose cotransporter, type 2 inhibitors (SGLT2i) are emerging as the gold standard for treatment of type 2 diabetes (T2D) with renal protective benefits independent of glucose lowering. We took a high-level approach to evaluate the effects of the SGLT2i, empagliflozin (EMPA) on renal metabolism and function in a prediabetic model of metabolic syndrome. Male and female 12-wk-old TallyHo (TH) mice, and their closest genetic lean strain (Swiss-Webster, SW) were treated with a high-milk-fat diet (HMFD) plu...
Source: American Journal of Physiology. Cell Physiology - April 11, 2024 Category: Cytology Authors: Blythe D Shepard Jennifer Chau Ryan Kurtz Avi Z Rosenberg Pinaki Sarder Samuel P Border Brandon Ginley Olga Rodriguez Chris Albanese Grace Knoer Aarenee Greene Aline M A De Souza Suman Ranjit Moshe Levi Carolyn M Ecelbarger Source Type: research
