Tailoring Transfection for Cardiomyocyte Cell Lines: Balancing Efficiency and Toxicity in Lipid versus Polymer-Based Transfection Methods in H9c2 and HL-1 Cells
In this study, we introduce meticulously crafted protocols to enhance the culture and transfection of HL-1 and H9c2 cells, emphasizing the reduction of cytotoxic effects while improving transfection efficiency. Through the examination of polymer-based and lipid-based transfection methods, we offer a comparative analysis that underscores the heightened efficiency and reduced toxicity of these approaches. Our research provides an extensive array of step-by-step procedures designed to foster robust cell cultures and outlines troubleshooting practices to rectify issues of low transfection rates. We discuss the merits and drawb...
Source: American Journal of Physiology. Heart and Circulatory Physiology - April 12, 2024 Category: Physiology Authors: Flobater I Gawargi Hamid R Shahshahan Paras K Mishra Source Type: research
The Association of Self-Reported Sleep and Circadian Measures with Glycemic Control and Diabetes Complications among Young Adults with Type 2 Diabetes
In conclusion, late bedtime on work/school days, rather than short sleep duration, daytime sleepiness, or poor sleep quality, was independently associated with loss of glycemic control in this longitudinal cohort of young adults with youth-onset type 2 diabetes.PMID:38607342 | DOI:10.1152/ajpheart.00550.2023 (Source: American Journal of Physiology. Heart and Circulatory Physiology)
Source: American Journal of Physiology. Heart and Circulatory Physiology - April 12, 2024 Category: Physiology Authors: Dorit Koren Kristen L Knutson Brian K Burke Kimberly L Drews Fida Bacha Lorraine Katz Marsha D Marcus Siripoom McKay Kristen Nadeau Babak Mokhlesi Source Type: research
Physiological impacts of atmospheric pollution: Effects of environmental air pollution on exercise
AbstractIn this review, we discuss some of the recent advances in our understanding of the physiology of the air pollution and exercise. The key areas covered include the effect of exercise intensity, the effects of pre-exposure to air pollution, acclimation to air pollution, and the utility of masks during exercise. Although higher intensity exercise leads to an increase in the inhaled dose of pollutants for a given distance traveled, the acute effects of (diesel exhaust) air pollution do not appear to be more pronounced. Second, exposure to air pollution outside of exercise bouts seems to have an effect on exercise respo...
Source: Physiological Reports - April 12, 2024 Category: Physiology Authors: Michael Stephen Koehle Tags: INVITED REVIEW Source Type: research
Effect of dietary chitosan on the growth performance, intestinal histology and growth ‐related gene expression in stellate sturgeon (Acipenser stellatus) juveniles
This study aimed to determine the impact of dietary chitosan on growth performance, whole-body proximate composition, growth-related gene expression, and intestinal histology in juvenileAcipenser stellatus. A total of 180 A. stellatus juveniles with an average weight of 31.90 ± 0.73 g were fed with diets containing 0 (control), 1.5, 3.0, 4.5, and 6.0 g chitosan.kg−1 basic diet for eight weeks. The findings revealed a significant enhancement in growth performance with rising chitosan concentrations. Furthermore, chitosan supplementation upregulated the expression of the growth hormone gene in both brain and l...
Source: Journal of Animal Physiology and Animal Nutrition - April 12, 2024 Category: Zoology Authors: Donya Zakeri,
Jamileh Pazooki,
Mahmoud Mohseni,
Shirin Jamshidi Tags: ORIGINAL ARTICLE Source Type: research
Considerations on static pressure gradients in closed circulatory systems
AbstractSiphons are devices that transport liquids uphill between two containers. It has been proposed that a siphon principle operates in closed circulatory systems, as best exemplified by the circulation of blood in mammals. This principle is supposed to ensure that no additional work is necessary to pump blood above the level of the heart, and that there is no gravitational static pressure gradient in the column of blood. The first statement is correct, while we demonstrate that, ignoring hydraulic resistance to blood flow, the static pressure gradient is equal to the hydrostatic gradient in a siphon model of blood circ...
Source: Physiological Reports - April 12, 2024 Category: Physiology Authors: Nato Popara,
Denis Cvitkovi ć,
Marinko Vilić,
Selim Pašić Tags: ORIGINAL ARTICLE Source Type: research
Intramuscular injection of mesenchymal stem cells augments basal muscle protein synthesis after bouts of resistance exercise in male mice
AbstractSkeletal muscle mass is critical for activities of daily living. Resistance training maintains or increases muscle mass, and various strategies maximize the training adaptation. Mesenchymal stem cells (MSCs) are multipotent cells with differential potency in skeletal muscle cells and the capacity to secrete growth factors. However, little is known regarding the effect of intramuscular injection of MSCs on basal muscle protein synthesis and catabolic systems after resistance training. Here, we measured changes in basal muscle protein synthesis, the ubiquitin-proteasome system, and autophagy-lysosome system-related f...
Source: Physiological Reports - April 12, 2024 Category: Physiology Authors: Junya Takegaki,
Kohei Sase,
Yusuke Kono,
Takuya Fujita,
Satoshi Konishi,
Satoshi Fujita Tags: ORIGINAL ARTICLE Source Type: research
Physiology education in China: the current situation and changes over the past 3 decades
As an experimental biological science, physiology has been taught as an integral component of medical curricula for a long time in China. The teaching effectiveness of physiology courses will directly affect s... (Source: BMC Medical Education)
Source: BMC Medical Education - April 12, 2024 Category: Universities & Medical Training Authors: Xuhong Wei, Ting Xu, Ruixian Guo, Zhi Tan and Wenjun Xin Tags: Research Source Type: research
Role of Anti-M üllerian Hormone in the Central Regulation of Fertility
Semin Reprod Med DOI: 10.1055/s-0044-1786050In recent years, the expanding roles of anti-Müllerian hormone (AMH) in various aspects of reproductive health have attracted significant attention. Initially recognized for its classical role in male sexual differentiation, AMH is produced postnatally by the Sertoli cells in the male testes and by the granulosa cells in the female ovaries. Traditionally, it was believed to primarily influence gonadal development and function. However, research over the last decade has unveiled novel actions of AMH beyond the gonads, specifically all along the hypothalamic–pituitary–gonadal ...
Source: Seminars in Reproductive Medicine - April 12, 2024 Category: Reproduction Medicine Authors: Cotellessa, Ludovica Giacobini, Paolo Tags: Review Article Source Type: research
Endothelial dysfunction influences augmented aortic hemodynamic responses to metaboreflex activation in postmenopausal women
ConclusionEndothelial dysfunction relates to augmented aortic pulsatile load during metaboreflex activation, which may increase cardiovascular risk in postmenopausal women. (Source: European Journal of Applied Physiology)
Source: European Journal of Applied Physiology - April 12, 2024 Category: Physiology Source Type: research
News at a glance: Jailed conservationists freed, physics titan dies, and diversity in anatomical illustrations
ASTRONOMY
Scientists flock to the eclipse
Tens of millions in North America turned their specially protected eyes to the skies on 8 April for several minutes of darkness when the Moon blocked the disk of the Sun, casting a shadow across Mexico, 15 U.S. states, and Canada. Researchers and citizen scientists seized the opportunity to study the corona—the wispy, outer layer of the Sun’s atmosphere—from the ground and in eclipse-chasing aircraft. They expected to see loops of plasma and other solar flares because the eclipse coincided with solar maximum, a period of high magnetic activity. Research...
Source: Science of Aging Knowledge Environment - April 11, 2024 Category: Geriatrics Source Type: research
A transformer-based approach for Arabic offline handwritten text recognition
AbstractHandwriting recognition is a challenging and critical problem in the fields of pattern recognition and machine learning, with applications spanning a wide range of domains. In this paper, we focus on the specific issue of recognizing offline Arabic handwritten text. Existing approaches typically utilize a combination of convolutional neural networks for image feature extraction and recurrent neural networks for temporal modeling, with connectionist temporal classification used for text generation. However, these methods suffer from a lack of parallelization due to the sequential nature of recurrent neural networks....
Source: European Journal of Applied Physiology - April 11, 2024 Category: Physiology Source Type: research
A cupping spots image enhancement algorithm based on LAA-CycleGAN
AbstractDuring the automatic cupping process, a LAA-CycleGAN-based image enhancement algorithm is proposed to address the issues of reduced image clarity and loss of detail features caused by fog adhering to the surface of the can body. Firstly, the generator contains a self-attention module to capture global features of the images; secondly, the discriminator introduces an auto-encoder to generate more stable images; finally, a perceptual loss term is added to optimize the network for better perception. Experiments were conducted on the collected cupping spots dataset, and the results showed that compared with DCP, Dehaze...
Source: European Journal of Applied Physiology - April 11, 2024 Category: Physiology Source Type: research
Radiological evaluation before and after treatment with an osseointegrated bone-anchor following major limb amputation —a guide for radiologists
This article acts as a guide for radiologists who may be tasked with providing the radiological information required by surgeons and prosthetists. We also look at the radiological appearances of complications that may arise in patients treated with an osseointegrated implant. Plain X-rays are used to screen patients who wish to undergo treatment. Limb-length X-rays are then used to measure the length of any residual bone, and comparisons can be made with the normal side (if present). From this, decisions about the likely size of the implant and the need for further amputation can be made. CT scans enable accurate assessmen...
Source: European Journal of Applied Physiology - April 11, 2024 Category: Physiology Source Type: research
Atomistic understanding of rough surface on the interfacial friction behavior during the chemical mechanical polishing process of diamond
AbstractThe roughness of the contact surface exerts a vital role in rubbing. It is still a significant challenge to understand the microscopic contact of the rough surface at the atomic level. Herein, the rough surface with a special root mean square (RMS) value is constructed by multivariate Weierstrass –Mandelbrot (W–M) function and the rubbing process during that the chemical mechanical polishing (CMP) process of diamond is mimicked utilizing the reactive force field molecular dynamics (ReaxFF MD) simulation. It is found that the contact areaA/A0 is positively related with the load, and the friction forceF depends o...
Source: European Journal of Applied Physiology - April 11, 2024 Category: Physiology Source Type: research
The effect of secondary phase on corrosion behaviors of the titanium –zirconium–molybdenum alloy
AbstractAs the range of applications for molybdenum and its alloys has expanded, the corrosive environment for molybdenum alloys has become more demanding. In the past, the content of doping elements has been studied to investigate their influence on the corrosion performance of titanium –zirconium–molybdenum (TZM) alloys. In this paper, it is considered that the second phase in the alloy is the main factor affecting the corrosion performance of TZM alloys. By comparing the corrosion behavior of molybdenum metal and TZM alloy, the effect of the secondary phase on corrosion behav iors of TZM alloy has been investigated....
Source: European Journal of Applied Physiology - April 11, 2024 Category: Physiology Source Type: research
