Viruses, Vol. 16, Pages 157: Molecular Survey on Porcine Parvoviruses (PPV1-7) and Their Association with Major Pathogens in Reproductive Failure Outbreaks in Northern Italy
Michele Drigo Successful reproductive performance is key to farm competitiveness in the global marketplace. Porcine parvovirus 1 (PPV1) has been identified as a major cause of reproductive failure, and since 2001 new species of porcine parvoviruses, namely PPV2–7, have been identified, although their role is not yet fully understood yet. The present study aimed to investigate PPVs’ presence in reproductive failure outbreaks occurring in 124 farms of northern Italy. Fetuses were collected from 338 sows between 2019 and 2021 and tested for PPVs by real-time PCR-based assays and for other virus...
Source: Viruses - January 21, 2024 Category: Virology Authors: Giulia Faustini Claudia Maria Tucciarone Giovanni Franzo Anna Donneschi Maria Beatrice Boniotti Giovanni Loris Alborali Michele Drigo Tags: Article Source Type: research

Feasibility of Domain Segmentation of B19V VP1u Using Intein Technology for Structural Studies
CONCLUSION: This method would allow for the selective incorporation of NMR-active isotopes into either of the VP1u domains, which can reduce signal overlap in NMR structural determination studies.PMID:38243925 | DOI:10.2174/0109298665277211231214065419 (Source: Protein and Peptide Letters)
Source: Protein and Peptide Letters - January 20, 2024 Category: Biochemistry Authors: Renuk Varayil Lakshmanan Mavis Agbandje-McKenna Robert McKenna Source Type: research

Biotechnological and virological analysis of canine parvovirus infections by C-reactive protein levels, serological, hematological and molecular techniques
Cell Mol Biol (Noisy-le-grand). 2023 Dec 10;69(13):279-286. doi: 10.14715/cmb/2023.69.13.41.ABSTRACTThe study aims to approach Canine Parvovirus (CPV) diagnosis using multi-method biotechnological techniques including molecular, serological, and hematological analyses. CPVs are causing severe global viral diseases with high dog mortality. Samples were taken from 52 unvaccinated dogs exhibiting symptoms between 2020 and 2021. These included stool, blood, serum, and patient data. CPV genomic DNA was extracted from fresh stools, with DNA concentration and purity measured using Nano drop-spectrophotometry. CPV genomic DNA was ...
Source: Cellular and Molecular Biology - January 1, 2024 Category: Molecular Biology Authors: Pinar Palanci Bilge Kaan Tekelioglu Source Type: research

Chloroplast display of subunit vaccines and their efficacy via oral administration
In this study, we innovatively utilized the transmembrane region of the pea Translocase of chloroplast 34 (TOC34) protein to display two subunit vaccines, capsid protein VP2 of Porcine parvovirus (PPV) and the heat-labile enterotoxin B (LTB) of Escherichia coli, on the surface of chloroplasts. Unlike microbial display techniques, chloroplast display circumvents antigen degradation in the stomach while retaining the size characteristic of microorganisms. Additionally, a co-expressed peptide adjuvant, antimicrobial peptide protegin-1 (PG1), was used to enhance the strength of oral immunization. Immunohistochemistry and tryps...
Source: International Journal of Biological Macromolecules - January 1, 2024 Category: Biochemistry Authors: Juan Han Ji-Feng Zhang Lu-Ya Hu Cheng-Run Wang Shun-Chang Wang Guopeng Miao Source Type: research

Biotechnological and virological analysis of canine parvovirus infections by C-reactive protein levels, serological, hematological and molecular techniques
Cell Mol Biol (Noisy-le-grand). 2023 Dec 10;69(13):279-286. doi: 10.14715/cmb/2023.69.13.41.ABSTRACTThe study aims to approach Canine Parvovirus (CPV) diagnosis using multi-method biotechnological techniques including molecular, serological, and hematological analyses. CPVs are causing severe global viral diseases with high dog mortality. Samples were taken from 52 unvaccinated dogs exhibiting symptoms between 2020 and 2021. These included stool, blood, serum, and patient data. CPV genomic DNA was extracted from fresh stools, with DNA concentration and purity measured using Nano drop-spectrophotometry. CPV genomic DNA was ...
Source: Mol Biol Cell - January 1, 2024 Category: Molecular Biology Authors: Pinar Palanci Bilge Kaan Tekelioglu Source Type: research

Biotechnological and virological analysis of canine parvovirus infections by C-reactive protein levels, serological, hematological and molecular techniques
Cell Mol Biol (Noisy-le-grand). 2023 Dec 10;69(13):279-286. doi: 10.14715/cmb/2023.69.13.41.ABSTRACTThe study aims to approach Canine Parvovirus (CPV) diagnosis using multi-method biotechnological techniques including molecular, serological, and hematological analyses. CPVs are causing severe global viral diseases with high dog mortality. Samples were taken from 52 unvaccinated dogs exhibiting symptoms between 2020 and 2021. These included stool, blood, serum, and patient data. CPV genomic DNA was extracted from fresh stools, with DNA concentration and purity measured using Nano drop-spectrophotometry. CPV genomic DNA was ...
Source: Cellular and Molecular Biology - January 1, 2024 Category: Molecular Biology Authors: Pinar Palanci Bilge Kaan Tekelioglu Source Type: research

Chloroplast display of subunit vaccines and their efficacy via oral administration
In this study, we innovatively utilized the transmembrane region of the pea Translocase of chloroplast 34 (TOC34) protein to display two subunit vaccines, capsid protein VP2 of Porcine parvovirus (PPV) and the heat-labile enterotoxin B (LTB) of Escherichia coli, on the surface of chloroplasts. Unlike microbial display techniques, chloroplast display circumvents antigen degradation in the stomach while retaining the size characteristic of microorganisms. Additionally, a co-expressed peptide adjuvant, antimicrobial peptide protegin-1 (PG1), was used to enhance the strength of oral immunization. Immunohistochemistry and tryps...
Source: International Journal of Biological Macromolecules - January 1, 2024 Category: Biochemistry Authors: Juan Han Ji-Feng Zhang Lu-Ya Hu Cheng-Run Wang Shun-Chang Wang Guopeng Miao Source Type: research

Biotechnological and virological analysis of canine parvovirus infections by C-reactive protein levels, serological, hematological and molecular techniques
Cell Mol Biol (Noisy-le-grand). 2023 Dec 10;69(13):279-286. doi: 10.14715/cmb/2023.69.13.41.ABSTRACTThe study aims to approach Canine Parvovirus (CPV) diagnosis using multi-method biotechnological techniques including molecular, serological, and hematological analyses. CPVs are causing severe global viral diseases with high dog mortality. Samples were taken from 52 unvaccinated dogs exhibiting symptoms between 2020 and 2021. These included stool, blood, serum, and patient data. CPV genomic DNA was extracted from fresh stools, with DNA concentration and purity measured using Nano drop-spectrophotometry. CPV genomic DNA was ...
Source: Cellular and Molecular Biology - January 1, 2024 Category: Molecular Biology Authors: Pinar Palanci Bilge Kaan Tekelioglu Source Type: research

Metapneumovirus: A Rare Agent Causing Myocarditis
Viral causes of myocarditis and cardiomyopathy are well established with known agents such as parvoviruses, enteroviruses, and adenoviruses. Human metapneumovirus (hMPV), belonging to the Pneumovirdiae family, was first discovered in 2001 primarily resulting in upper and lower respiratory tract infections - most common in children, older individuals, and those with a weakened or compromised immune system. Once infected, it has been reported to cause pneumonia, croup, otitis media, and conjunctivitis. (Source: Journal of Cardiac Failure)
Source: Journal of Cardiac Failure - January 1, 2024 Category: Cardiology Authors: Radha Gopalan, Andres Vargas Rodriguez, McEwan Hopkins, Ambar Andrade, Ryan Ung, Anantharam Kayla, Marc Silver Tags: 398 Source Type: research

Genetic characterization of parvoviruses identified in stray cats in Nigeria
In this study, the presence and genetic characteristics of parvoviruses circulating among cats in Nigeria are reported. Faecal samples of stray cats from live animal markets in southwest (Oyo and Osun States) and north-central (Kwara State) Nigeria were screened for the presence of parvoviral DNA using a qPCR. Positive samples were further characterized using a qPCR based on minor groove binder probes. Overall, 85/102 (83.3%) stray cats tested positive for feline panleukopenia virus (FPV) DNA and one cat was co-infected with canine parvovirus-2 type a. Sequence analysis of the complete capsid region of 15 Nigerian FPV stra...
Source: Acta Tropica - December 25, 2023 Category: Infectious Diseases Authors: Daniel Oladimeji Oluwayelu Costantina Desario Eunice Temilade Babalola Annamaria Pratelli Oluwafemi Babatunde Daodu Gabriella Elia Solomon Olawole Odemuyiwa Canio Buonavoglia Nicola Decaro Georgia Diakoudi Source Type: research