Association between Parvovirus B19 and thyroid/celiac autoantibodies among T1DM pediatric patients
CONCLUSIONS: The prevalence of autoantibodies against the thyroid was higher among the seropositive Parvovirus B19 children with T1DM. A positive association between the prevalence of autoantibodies against thyroid disease and the increase in the duration of diabetes was also noted. Hence, periodic screening of T1DM patients for B19 antibodies and autoantibodies for thyroid is crucial.PMID:38497882 | DOI:10.26355/eurrev_202403_35614 (Source: European Review for Medical and Pharmacological Sciences)
Source: European Review for Medical and Pharmacological Sciences - March 18, 2024 Category: Drugs & Pharmacology Authors: H C Chandramoorthy A A Shati A A Aseeri A Subramanian A Alhanshani E A H Almohseny R Ram Mani A Kumar R A Ali Moosa A Al-Hakami K Chidambaram Source Type: research

Development of a time-resolved fluorescence immunoassay kit for detecting canine coronavirus and parvovirus through double labeling
This study aimed to ... (Source: Virology Journal)
Source: Virology Journal - March 11, 2024 Category: Virology Authors: Laiqing Li, Cuicui Chen, Huankun Liang, Wenqi Dong, V. N. Leontiev and Igor Vitalievich Voytov Tags: Methodology Source Type: research

Establishment of RPA-Cas12a-Based Fluorescence Assay for Rapid Detection of Feline Parvovirus
In this study, we incorporated recombinase polymerase amplification (RPA) and Cas12a-mediated detection and developed an RPA-Cas12a-based real-time or end-point fluorescence detection method to identify the NS1 gene of FPV. The total time of RPA-Cas12a-based fluorescence assay is approximately 25 min. The assay presented a limit of detection (LOD) of 1 copies/μl (25 copies/per reaction), with no cross-reactivity with several feline pathogens. The clinical performance of the assay was examined using total genomic DNA purified from 60 clinical specimens and then compared to results obtained with qPCR detection of FPV with 9...
Source: Polish Journal of Microbiology - March 4, 2024 Category: Microbiology Authors: Ting Wang Hao Zeng Qiming Liu Weidong Qian Yongdong Li Jian Liu Rong Xu Source Type: research

Establishment of RPA-Cas12a-Based Fluorescence Assay for Rapid Detection of Feline Parvovirus
In this study, we incorporated recombinase polymerase amplification (RPA) and Cas12a-mediated detection and developed an RPA-Cas12a-based real-time or end-point fluorescence detection method to identify the NS1 gene of FPV. The total time of RPA-Cas12a-based fluorescence assay is approximately 25 min. The assay presented a limit of detection (LOD) of 1 copies/μl (25 copies/per reaction), with no cross-reactivity with several feline pathogens. The clinical performance of the assay was examined using total genomic DNA purified from 60 clinical specimens and then compared to results obtained with qPCR detection of FPV with 9...
Source: Polish Journal of Microbiology - March 4, 2024 Category: Microbiology Authors: Ting Wang Hao Zeng Qiming Liu Weidong Qian Yongdong Li Jian Liu Rong Xu Source Type: research