Hemolytic anemia in a two-year-old child: A case of multiple red blood cell abnormalities
Ann Biol Clin (Paris). 2023 Nov 29;81(5). doi: 10.1684/abc.2023.1843. Online ahead of print.ABSTRACTThe discovery of hemolytic anemia requires a meticulous investigation to determine its etiology. Among patients of African origin, it is not uncommon to find multiple constitutional red blood cell abnormalities, which can complicate diagnosis. We herein describe the case of a two-year-old child presenting with acute hemolytic anemia. A G6PD deficiency, hereditary spherocytosis, and a sickle cell trait A/S were simultaneously identified, all within the context of a primary infection with Parvovirus B19. This virus commonly tr...
Source: Annales de Biologie Clinique - November 29, 2023 Category: Biochemistry Authors: Romain Ravel-Chapuis L éo Mottin Ma ïssa Souissi Agn ès Lahary Victor Bob ée Source Type: research

Development and application of a multiplex PCR method for the simultaneous detection of goose parvovirus, waterfowl reovirus, and goose astrovirus in Muscovy ducks
J Virol Methods. 2023 Nov 27:114857. doi: 10.1016/j.jviromet.2023.114857. Online ahead of print.ABSTRACTA multiplex polymerase chain reaction (PCR) method was developed to detect and distinguish goose parvovirus (GPV), waterfowl reovirus (WRV), and goose astrovirus (GAstV). Three pairs of primers were designed based on conserved regions in the genomic sequences of these enteric viruses and were used to specifically amplify targeted fragments of 493bp from the viral protein 3 (VP3) gene of GPV, 300bp from the sigma A-encoding gene of WRV, and 156bp from the capsid protein-encoding gene of GAstV. The results showed that the ...
Source: Journal of Virological Methods - November 29, 2023 Category: Virology Authors: Shizhong Zhang Hui Dong Fengqiang Lin Xiaoxia Cheng Xiaoli Zhu Dandan Jiang Shifeng Xiao Shaoying Chen Shilong Chen Shao Wang Source Type: research

Hemolytic anemia in a two-year-old child: A case of multiple red blood cell abnormalities
Ann Biol Clin (Paris). 2023 Nov 29;81(5). doi: 10.1684/abc.2023.1843. Online ahead of print.ABSTRACTThe discovery of hemolytic anemia requires a meticulous investigation to determine its etiology. Among patients of African origin, it is not uncommon to find multiple constitutional red blood cell abnormalities, which can complicate diagnosis. We herein describe the case of a two-year-old child presenting with acute hemolytic anemia. A G6PD deficiency, hereditary spherocytosis, and a sickle cell trait A/S were simultaneously identified, all within the context of a primary infection with Parvovirus B19. This virus commonly tr...
Source: Annales de Biologie Clinique - November 29, 2023 Category: Biochemistry Authors: Romain Ravel-Chapuis L éo Mottin Ma ïssa Souissi Agn ès Lahary Victor Bob ée Source Type: research

Development and application of a multiplex PCR method for the simultaneous detection of goose parvovirus, waterfowl reovirus, and goose astrovirus in Muscovy ducks
J Virol Methods. 2023 Nov 27:114857. doi: 10.1016/j.jviromet.2023.114857. Online ahead of print.ABSTRACTA multiplex polymerase chain reaction (PCR) method was developed to detect and distinguish goose parvovirus (GPV), waterfowl reovirus (WRV), and goose astrovirus (GAstV). Three pairs of primers were designed based on conserved regions in the genomic sequences of these enteric viruses and were used to specifically amplify targeted fragments of 493bp from the viral protein 3 (VP3) gene of GPV, 300bp from the sigma A-encoding gene of WRV, and 156bp from the capsid protein-encoding gene of GAstV. The results showed that the ...
Source: Journal of Virological Methods - November 29, 2023 Category: Virology Authors: Shizhong Zhang Hui Dong Fengqiang Lin Xiaoxia Cheng Xiaoli Zhu Dandan Jiang Shifeng Xiao Shaoying Chen Shilong Chen Shao Wang Source Type: research

Hemolytic anemia in a two-year-old child: A case of multiple red blood cell abnormalities
Ann Biol Clin (Paris). 2023 Nov 29;81(5). doi: 10.1684/abc.2023.1843. Online ahead of print.ABSTRACTThe discovery of hemolytic anemia requires a meticulous investigation to determine its etiology. Among patients of African origin, it is not uncommon to find multiple constitutional red blood cell abnormalities, which can complicate diagnosis. We herein describe the case of a two-year-old child presenting with acute hemolytic anemia. A G6PD deficiency, hereditary spherocytosis, and a sickle cell trait A/S were simultaneously identified, all within the context of a primary infection with Parvovirus B19. This virus commonly tr...
Source: Annales de Biologie Clinique - November 29, 2023 Category: Biochemistry Authors: Romain Ravel-Chapuis L éo Mottin Ma ïssa Souissi Agn ès Lahary Victor Bob ée Source Type: research

Development and application of a multiplex PCR method for the simultaneous detection of goose parvovirus, waterfowl reovirus, and goose astrovirus in Muscovy ducks
J Virol Methods. 2023 Nov 27:114857. doi: 10.1016/j.jviromet.2023.114857. Online ahead of print.ABSTRACTA multiplex polymerase chain reaction (PCR) method was developed to detect and distinguish goose parvovirus (GPV), waterfowl reovirus (WRV), and goose astrovirus (GAstV). Three pairs of primers were designed based on conserved regions in the genomic sequences of these enteric viruses and were used to specifically amplify targeted fragments of 493bp from the viral protein 3 (VP3) gene of GPV, 300bp from the sigma A-encoding gene of WRV, and 156bp from the capsid protein-encoding gene of GAstV. The results showed that the ...
Source: Journal of Virological Methods - November 29, 2023 Category: Virology Authors: Shizhong Zhang Hui Dong Fengqiang Lin Xiaoxia Cheng Xiaoli Zhu Dandan Jiang Shifeng Xiao Shaoying Chen Shilong Chen Shao Wang Source Type: research

Hemolytic anemia in a two-year-old child: A case of multiple red blood cell abnormalities
Ann Biol Clin (Paris). 2023 Nov 29;81(5). doi: 10.1684/abc.2023.1843. Online ahead of print.ABSTRACTThe discovery of hemolytic anemia requires a meticulous investigation to determine its etiology. Among patients of African origin, it is not uncommon to find multiple constitutional red blood cell abnormalities, which can complicate diagnosis. We herein describe the case of a two-year-old child presenting with acute hemolytic anemia. A G6PD deficiency, hereditary spherocytosis, and a sickle cell trait A/S were simultaneously identified, all within the context of a primary infection with Parvovirus B19. This virus commonly tr...
Source: Annales de Biologie Clinique - November 29, 2023 Category: Biochemistry Authors: Romain Ravel-Chapuis L éo Mottin Ma ïssa Souissi Agn ès Lahary Victor Bob ée Source Type: research

Development and application of a multiplex PCR method for the simultaneous detection of goose parvovirus, waterfowl reovirus, and goose astrovirus in Muscovy ducks
J Virol Methods. 2023 Nov 27:114857. doi: 10.1016/j.jviromet.2023.114857. Online ahead of print.ABSTRACTA multiplex polymerase chain reaction (PCR) method was developed to detect and distinguish goose parvovirus (GPV), waterfowl reovirus (WRV), and goose astrovirus (GAstV). Three pairs of primers were designed based on conserved regions in the genomic sequences of these enteric viruses and were used to specifically amplify targeted fragments of 493bp from the viral protein 3 (VP3) gene of GPV, 300bp from the sigma A-encoding gene of WRV, and 156bp from the capsid protein-encoding gene of GAstV. The results showed that the ...
Source: Journal of Virological Methods - November 29, 2023 Category: Virology Authors: Shizhong Zhang Hui Dong Fengqiang Lin Xiaoxia Cheng Xiaoli Zhu Dandan Jiang Shifeng Xiao Shaoying Chen Shilong Chen Shao Wang Source Type: research

Hemolytic anemia in a two-year-old child: A case of multiple red blood cell abnormalities
Ann Biol Clin (Paris). 2023 Nov 29;81(5). doi: 10.1684/abc.2023.1843. Online ahead of print.ABSTRACTThe discovery of hemolytic anemia requires a meticulous investigation to determine its etiology. Among patients of African origin, it is not uncommon to find multiple constitutional red blood cell abnormalities, which can complicate diagnosis. We herein describe the case of a two-year-old child presenting with acute hemolytic anemia. A G6PD deficiency, hereditary spherocytosis, and a sickle cell trait A/S were simultaneously identified, all within the context of a primary infection with Parvovirus B19. This virus commonly tr...
Source: Annales de Biologie Clinique - November 29, 2023 Category: Biochemistry Authors: Romain Ravel-Chapuis L éo Mottin Ma ïssa Souissi Agn ès Lahary Victor Bob ée Source Type: research

Development and application of a multiplex PCR method for the simultaneous detection of goose parvovirus, waterfowl reovirus, and goose astrovirus in Muscovy ducks
J Virol Methods. 2023 Nov 27:114857. doi: 10.1016/j.jviromet.2023.114857. Online ahead of print.ABSTRACTA multiplex polymerase chain reaction (PCR) method was developed to detect and distinguish goose parvovirus (GPV), waterfowl reovirus (WRV), and goose astrovirus (GAstV). Three pairs of primers were designed based on conserved regions in the genomic sequences of these enteric viruses and were used to specifically amplify targeted fragments of 493bp from the viral protein 3 (VP3) gene of GPV, 300bp from the sigma A-encoding gene of WRV, and 156bp from the capsid protein-encoding gene of GAstV. The results showed that the ...
Source: Journal of Virological Methods - November 29, 2023 Category: Virology Authors: Shizhong Zhang Hui Dong Fengqiang Lin Xiaoxia Cheng Xiaoli Zhu Dandan Jiang Shifeng Xiao Shaoying Chen Shilong Chen Shao Wang Source Type: research

Hemolytic anemia in a two-year-old child: A case of multiple red blood cell abnormalities
Ann Biol Clin (Paris). 2023 Nov 29;81(5). doi: 10.1684/abc.2023.1843. Online ahead of print.ABSTRACTThe discovery of hemolytic anemia requires a meticulous investigation to determine its etiology. Among patients of African origin, it is not uncommon to find multiple constitutional red blood cell abnormalities, which can complicate diagnosis. We herein describe the case of a two-year-old child presenting with acute hemolytic anemia. A G6PD deficiency, hereditary spherocytosis, and a sickle cell trait A/S were simultaneously identified, all within the context of a primary infection with Parvovirus B19. This virus commonly tr...
Source: Annales de Biologie Clinique - November 29, 2023 Category: Biochemistry Authors: Romain Ravel-Chapuis L éo Mottin Ma ïssa Souissi Agn ès Lahary Victor Bob ée Source Type: research

Development and application of a multiplex PCR method for the simultaneous detection of goose parvovirus, waterfowl reovirus, and goose astrovirus in Muscovy ducks
J Virol Methods. 2023 Nov 27:114857. doi: 10.1016/j.jviromet.2023.114857. Online ahead of print.ABSTRACTA multiplex polymerase chain reaction (PCR) method was developed to detect and distinguish goose parvovirus (GPV), waterfowl reovirus (WRV), and goose astrovirus (GAstV). Three pairs of primers were designed based on conserved regions in the genomic sequences of these enteric viruses and were used to specifically amplify targeted fragments of 493bp from the viral protein 3 (VP3) gene of GPV, 300bp from the sigma A-encoding gene of WRV, and 156bp from the capsid protein-encoding gene of GAstV. The results showed that the ...
Source: Journal of Virological Methods - November 29, 2023 Category: Virology Authors: Shizhong Zhang Hui Dong Fengqiang Lin Xiaoxia Cheng Xiaoli Zhu Dandan Jiang Shifeng Xiao Shaoying Chen Shilong Chen Shao Wang Source Type: research

Hemolytic anemia in a two-year-old child: A case of multiple red blood cell abnormalities
Ann Biol Clin (Paris). 2023 Nov 29;81(5). doi: 10.1684/abc.2023.1843. Online ahead of print.ABSTRACTThe discovery of hemolytic anemia requires a meticulous investigation to determine its etiology. Among patients of African origin, it is not uncommon to find multiple constitutional red blood cell abnormalities, which can complicate diagnosis. We herein describe the case of a two-year-old child presenting with acute hemolytic anemia. A G6PD deficiency, hereditary spherocytosis, and a sickle cell trait A/S were simultaneously identified, all within the context of a primary infection with Parvovirus B19. This virus commonly tr...
Source: Annales de Biologie Clinique - November 29, 2023 Category: Biochemistry Authors: Romain Ravel-Chapuis L éo Mottin Ma ïssa Souissi Agn ès Lahary Victor Bob ée Source Type: research

Hemolytic anemia in a two-year-old child: A case of multiple red blood cell abnormalities
Ann Biol Clin (Paris). 2023 Nov 29;81(5). doi: 10.1684/abc.2023.1843. Online ahead of print.ABSTRACTThe discovery of hemolytic anemia requires a meticulous investigation to determine its etiology. Among patients of African origin, it is not uncommon to find multiple constitutional red blood cell abnormalities, which can complicate diagnosis. We herein describe the case of a two-year-old child presenting with acute hemolytic anemia. A G6PD deficiency, hereditary spherocytosis, and a sickle cell trait A/S were simultaneously identified, all within the context of a primary infection with Parvovirus B19. This virus commonly tr...
Source: Annales de Biologie Clinique - November 29, 2023 Category: Biochemistry Authors: Romain Ravel-Chapuis L éo Mottin Ma ïssa Souissi Agn ès Lahary Victor Bob ée Source Type: research

Hemolytic anemia in a two-year-old child: A case of multiple red blood cell abnormalities
Ann Biol Clin (Paris). 2023 Nov 29;81(5). doi: 10.1684/abc.2023.1843. Online ahead of print.ABSTRACTThe discovery of hemolytic anemia requires a meticulous investigation to determine its etiology. Among patients of African origin, it is not uncommon to find multiple constitutional red blood cell abnormalities, which can complicate diagnosis. We herein describe the case of a two-year-old child presenting with acute hemolytic anemia. A G6PD deficiency, hereditary spherocytosis, and a sickle cell trait A/S were simultaneously identified, all within the context of a primary infection with Parvovirus B19. This virus commonly tr...
Source: Annales de Biologie Clinique - November 29, 2023 Category: Biochemistry Authors: Romain Ravel-Chapuis L éo Mottin Ma ïssa Souissi Agn ès Lahary Victor Bob ée Source Type: research