Chloroplast display of subunit vaccines and their efficacy via oral administration
In this study, we innovatively utilized the transmembrane region of the pea Translocase of chloroplast 34 (TOC34) protein to display two subunit vaccines, capsid protein VP2 of Porcine parvovirus (PPV) and the heat-labile enterotoxin B (LTB) of Escherichia coli, on the surface of chloroplasts. Unlike microbial display techniques, chloroplast display circumvents antigen degradation in the stomach while retaining the size characteristic of microorganisms. Additionally, a co-expressed peptide adjuvant, antimicrobial peptide protegin-1 (PG1), was used to enhance the strength of oral immunization. Immunohistochemistry and tryps...
Source: International Journal of Biological Macromolecules - January 1, 2024 Category: Biochemistry Authors: Juan Han Ji-Feng Zhang Lu-Ya Hu Cheng-Run Wang Shun-Chang Wang Guopeng Miao Source Type: research
Biotechnological and virological analysis of canine parvovirus infections by C-reactive protein levels, serological, hematological and molecular techniques
Cell Mol Biol (Noisy-le-grand). 2023 Dec 10;69(13):279-286. doi: 10.14715/cmb/2023.69.13.41.ABSTRACTThe study aims to approach Canine Parvovirus (CPV) diagnosis using multi-method biotechnological techniques including molecular, serological, and hematological analyses. CPVs are causing severe global viral diseases with high dog mortality. Samples were taken from 52 unvaccinated dogs exhibiting symptoms between 2020 and 2021. These included stool, blood, serum, and patient data. CPV genomic DNA was extracted from fresh stools, with DNA concentration and purity measured using Nano drop-spectrophotometry. CPV genomic DNA was ...
Source: Cellular and Molecular Biology - January 1, 2024 Category: Molecular Biology Authors: Pinar Palanci Bilge Kaan Tekelioglu Source Type: research
Metapneumovirus: A Rare Agent Causing Myocarditis
Viral causes of myocarditis and cardiomyopathy are well established with known agents such as parvoviruses, enteroviruses, and adenoviruses. Human metapneumovirus (hMPV), belonging to the Pneumovirdiae family, was first discovered in 2001 primarily resulting in upper and lower respiratory tract infections - most common in children, older individuals, and those with a weakened or compromised immune system. Once infected, it has been reported to cause pneumonia, croup, otitis media, and conjunctivitis. (Source: Journal of Cardiac Failure)
Source: Journal of Cardiac Failure - January 1, 2024 Category: Cardiology Authors: Radha Gopalan, Andres Vargas Rodriguez, McEwan Hopkins, Ambar Andrade, Ryan Ung, Anantharam Kayla, Marc Silver Tags: 398 Source Type: research
Genetic characterization of parvoviruses identified in stray cats in Nigeria
In this study, the presence and genetic characteristics of parvoviruses circulating among cats in Nigeria are reported. Faecal samples of stray cats from live animal markets in southwest (Oyo and Osun States) and north-central (Kwara State) Nigeria were screened for the presence of parvoviral DNA using a qPCR. Positive samples were further characterized using a qPCR based on minor groove binder probes. Overall, 85/102 (83.3%) stray cats tested positive for feline panleukopenia virus (FPV) DNA and one cat was co-infected with canine parvovirus-2 type a. Sequence analysis of the complete capsid region of 15 Nigerian FPV stra...
Source: Acta Tropica - December 25, 2023 Category: Infectious Diseases Authors: Daniel Oladimeji Oluwayelu Costantina Desario Eunice Temilade Babalola Annamaria Pratelli Oluwafemi Babatunde Daodu Gabriella Elia Solomon Olawole Odemuyiwa Canio Buonavoglia Nicola Decaro Georgia Diakoudi Source Type: research
Genetic characterization of parvoviruses identified in stray cats in Nigeria
In this study, the presence and genetic characteristics of parvoviruses circulating among cats in Nigeria are reported. Faecal samples of stray cats from live animal markets in southwest (Oyo and Osun States) and north-central (Kwara State) Nigeria were screened for the presence of parvoviral DNA using a qPCR. Positive samples were further characterized using a qPCR based on minor groove binder probes. Overall, 85/102 (83.3%) stray cats tested positive for feline panleukopenia virus (FPV) DNA and one cat was co-infected with canine parvovirus-2 type a. Sequence analysis of the complete capsid region of 15 Nigerian FPV stra...
Source: Acta Tropica - December 25, 2023 Category: Infectious Diseases Authors: Daniel Oladimeji Oluwayelu Costantina Desario Eunice Temilade Babalola Annamaria Pratelli Oluwafemi Babatunde Daodu Gabriella Elia Solomon Olawole Odemuyiwa Canio Buonavoglia Nicola Decaro Georgia Diakoudi Source Type: research
Genetic characterization of parvoviruses identified in stray cats in Nigeria
In this study, the presence and genetic characteristics of parvoviruses circulating among cats in Nigeria are reported. Faecal samples of stray cats from live animal markets in southwestern (Oyo and Osun States) and north-central (Kwara State) Nigeria were screened for the presence of parvoviral DNA using a qPCR. Positive samples were further characterized using a qPCR based on minor groove binder probes. Overall, 85/102 (83.3 %) stray cats tested positive for feline panleukopenia virus (FPV) DNA and one cat was co-infected with canine parvovirus-2 type a. Sequence analysis of the complete capsid region of 15 Nigerian FPV ...
Source: Acta Tropica - December 25, 2023 Category: Infectious Diseases Authors: Daniel Oladimeji Oluwayelu Costantina Desario Eunice Temilade Babalola Annamaria Pratelli Oluwafemi Babatunde Daodu Gabriella Elia Solomon Olawole Odemuyiwa Canio Buonavoglia Nicola Decaro Georgia Diakoudi Source Type: research
Parvovirus in Kidney Transplant Recipients: A Single-Center Experience
CONCLUSIONS: Parvovirus B19 is a relatively underreported disease in renal transplant recipients, with patients presenting with anemia and the disease causing transient graft dysfunction. Parvovirus B19 infection responds well to a decrease in immunosuppression and intravenous immunoglobulin therapy.PMID:38140930 | DOI:10.6002/ect.2023.0127 (Source: Experimental and Clinical Transplantation : official journal of the Middle East Society for Organ Transplantation)
Source: Experimental and Clinical Transplantation : official journal of the Middle East Society for Organ Transplantation - December 23, 2023 Category: Transplant Surgery Authors: Priyash Tambi Ruchir Dave Vivek Kute Himanshu V Patel Shivam Shah Rahul Yadav Source Type: research
Parvovirus in Kidney Transplant Recipients: A Single-Center Experience
CONCLUSIONS: Parvovirus B19 is a relatively underreported disease in renal transplant recipients, with patients presenting with anemia and the disease causing transient graft dysfunction. Parvovirus B19 infection responds well to a decrease in immunosuppression and intravenous immunoglobulin therapy.PMID:38140930 | DOI:10.6002/ect.2023.0127 (Source: Experimental and Clinical Transplantation : official journal of the Middle East Society for Organ Transplantation)
Source: Experimental and Clinical Transplantation : official journal of the Middle East Society for Organ Transplantation - December 23, 2023 Category: Transplant Surgery Authors: Priyash Tambi Ruchir Dave Vivek Kute Himanshu V Patel Shivam Shah Rahul Yadav Source Type: research
Parvovirus in Kidney Transplant Recipients: A Single-Center Experience
CONCLUSIONS: Parvovirus B19 is a relatively underreported disease in renal transplant recipients, with patients presenting with anemia and the disease causing transient graft dysfunction. Parvovirus B19 infection responds well to a decrease in immunosuppression and intravenous immunoglobulin therapy.PMID:38140930 | DOI:10.6002/ect.2023.0127 (Source: Experimental and Clinical Transplantation : official journal of the Middle East Society for Organ Transplantation)
Source: Experimental and Clinical Transplantation : official journal of the Middle East Society for Organ Transplantation - December 23, 2023 Category: Transplant Surgery Authors: Priyash Tambi Ruchir Dave Vivek Kute Himanshu V Patel Shivam Shah Rahul Yadav Source Type: research
Parvovirus in Kidney Transplant Recipients: A Single-Center Experience
CONCLUSIONS: Parvovirus B19 is a relatively underreported disease in renal transplant recipients, with patients presenting with anemia and the disease causing transient graft dysfunction. Parvovirus B19 infection responds well to a decrease in immunosuppression and intravenous immunoglobulin therapy.PMID:38140930 | DOI:10.6002/ect.2023.0127 (Source: Experimental and Clinical Transplantation : official journal of the Middle East Society for Organ Transplantation)
Source: Experimental and Clinical Transplantation : official journal of the Middle East Society for Organ Transplantation - December 23, 2023 Category: Transplant Surgery Authors: Priyash Tambi Ruchir Dave Vivek Kute Himanshu V Patel Shivam Shah Rahul Yadav Source Type: research
Design Rules for the Sequestration of Viruses into Polypeptide Complex Coacervates
Biomacromolecules. 2023 Dec 16. doi: 10.1021/acs.biomac.3c00938. Online ahead of print.ABSTRACTEncapsulation is a strategy that has been used to facilitate the delivery and increase the stability of proteins and viruses. Here, we investigate the encapsulation of viruses via complex coacervation, which is a liquid-liquid phase separation resulting from the complexation of oppositely charged polymers. In particular, we utilized polypeptide-based coacervates and explored the effects of peptide chemistry, chain length, charge patterning, and hydrophobicity to better understand the effects of the coacervating polypeptides on vi...
Source: Biomacromolecules - December 16, 2023 Category: Biochemistry Authors: Pratik U Joshi Claire Decker Xianci Zeng Arvind Sathyavageeswaran Sarah L Perry Caryn L Heldt Source Type: research
Design Rules for the Sequestration of Viruses into Polypeptide Complex Coacervates
Biomacromolecules. 2023 Dec 16. doi: 10.1021/acs.biomac.3c00938. Online ahead of print.ABSTRACTEncapsulation is a strategy that has been used to facilitate the delivery and increase the stability of proteins and viruses. Here, we investigate the encapsulation of viruses via complex coacervation, which is a liquid-liquid phase separation resulting from the complexation of oppositely charged polymers. In particular, we utilized polypeptide-based coacervates and explored the effects of peptide chemistry, chain length, charge patterning, and hydrophobicity to better understand the effects of the coacervating polypeptides on vi...
Source: Biomacromolecules - December 16, 2023 Category: Biochemistry Authors: Pratik U Joshi Claire Decker Xianci Zeng Arvind Sathyavageeswaran Sarah L Perry Caryn L Heldt Source Type: research
