Development of Digital Droplet PCR Targeting the Influenza H3N2 Oseltamivir-Resistant E119V Mutation and Its Performance through the Use of Reverse Genetics Mutants
In this study, based on an existing reverse transcriptase real-time PCR (RT-qPCR) assay, we developed a reverse transcriptase droplet digital PCR assay (RT-ddPCR) to detect and quantify the frequency of the E119V-NA mutation. Furthermore, reverse genetics viruses carrying this mutation were created to test the performance of the RT-ddPCR assay and compare it to the standard phenotypic NA assay. We also discuss the advantage of using an RT-ddPCR instead of qPCR method in the context of viral diagnostics and surveillance.PMID:36975535 | PMC:PMC10047791 | DOI:10.3390/cimb45030165 (Source: Current Issues in Molecular Biology)
Source: Current Issues in Molecular Biology - March 28, 2023 Category: Molecular Biology Authors: Laura A E Van Poelvoorde Fran çois E Dufrasne Steven Van Gucht Xavier Saelens Nancy H C Roosens Source Type: research
Development of Digital Droplet PCR Targeting the Influenza H3N2 Oseltamivir-Resistant E119V Mutation and Its Performance through the Use of Reverse Genetics Mutants
In this study, based on an existing reverse transcriptase real-time PCR (RT-qPCR) assay, we developed a reverse transcriptase droplet digital PCR assay (RT-ddPCR) to detect and quantify the frequency of the E119V-NA mutation. Furthermore, reverse genetics viruses carrying this mutation were created to test the performance of the RT-ddPCR assay and compare it to the standard phenotypic NA assay. We also discuss the advantage of using an RT-ddPCR instead of qPCR method in the context of viral diagnostics and surveillance.PMID:36975535 | PMC:PMC10047791 | DOI:10.3390/cimb45030165 (Source: Current Issues in Molecular Biology)
Source: Current Issues in Molecular Biology - March 28, 2023 Category: Molecular Biology Authors: Laura A E Van Poelvoorde Fran çois E Dufrasne Steven Van Gucht Xavier Saelens Nancy H C Roosens Source Type: research
Rapid Detection of Influenza Outbreaks in Long Term Care Facilities Reduces Emergency Room Visits and Hospitalization
Conclusions: The use of low threshold criteria to trigger nursing staff-initiated testing for influenza with RIDT resulted in increased prophylactic use of oseltamivir. There were significant reductions in the rates of all-cause ED visits (22% decline), hospitalizations (21% decline), and hospital length of stay (36% decline) across three combined influenza seasons. No significant differences were noted in respiratory-associated and all-cause deaths between intervention and control sites. This feasible, and low-cost intervention may provide significant benefit and should be further tested in other settings.PMID:36944078 | ...
Source: Annals of Family Medicine - March 21, 2023 Category: Primary Care Authors: Jonathan Temte Mary Checovich Marlon Mundt Shari Barlow Irene Hamrick Erik Reisdorf Source Type: research
Rapid Detection of Influenza Outbreaks in Long Term Care Facilities Reduces Emergency Room Visits and Hospitalization
Conclusions: The use of low threshold criteria to trigger nursing staff-initiated testing for influenza with RIDT resulted in increased prophylactic use of oseltamivir. There were significant reductions in the rates of all-cause ED visits (22% decline), hospitalizations (21% decline), and hospital length of stay (36% decline) across three combined influenza seasons. No significant differences were noted in respiratory-associated and all-cause deaths between intervention and control sites. This feasible, and low-cost intervention may provide significant benefit and should be further tested in other settings.PMID:36944078 | ...
Source: Annals of Family Medicine - March 21, 2023 Category: Primary Care Authors: Jonathan Temte Mary Checovich Marlon Mundt Shari Barlow Irene Hamrick Erik Reisdorf Source Type: research
Rapid Detection of Influenza Outbreaks in Long Term Care Facilities Reduces Emergency Room Visits and Hospitalization
Conclusions: The use of low threshold criteria to trigger nursing staff-initiated testing for influenza with RIDT resulted in increased prophylactic use of oseltamivir. There were significant reductions in the rates of all-cause ED visits (22% decline), hospitalizations (21% decline), and hospital length of stay (36% decline) across three combined influenza seasons. No significant differences were noted in respiratory-associated and all-cause deaths between intervention and control sites. This feasible, and low-cost intervention may provide significant benefit and should be further tested in other settings.PMID:36944078 | ...
Source: Annals of Family Medicine - March 21, 2023 Category: Primary Care Authors: Jonathan Temte Mary Checovich Marlon Mundt Shari Barlow Irene Hamrick Erik Reisdorf Source Type: research
Rapid Detection of Influenza Outbreaks in Long Term Care Facilities Reduces Emergency Room Visits and Hospitalization
Conclusions: The use of low threshold criteria to trigger nursing staff-initiated testing for influenza with RIDT resulted in increased prophylactic use of oseltamivir. There were significant reductions in the rates of all-cause ED visits (22% decline), hospitalizations (21% decline), and hospital length of stay (36% decline) across three combined influenza seasons. No significant differences were noted in respiratory-associated and all-cause deaths between intervention and control sites. This feasible, and low-cost intervention may provide significant benefit and should be further tested in other settings.PMID:36944078 | ...
Source: Annals of Family Medicine - March 21, 2023 Category: Primary Care Authors: Jonathan Temte Mary Checovich Marlon Mundt Shari Barlow Irene Hamrick Erik Reisdorf Source Type: research
Rapid Detection of Influenza Outbreaks in Long Term Care Facilities Reduces Emergency Room Visits and Hospitalization
Conclusions: The use of low threshold criteria to trigger nursing staff-initiated testing for influenza with RIDT resulted in increased prophylactic use of oseltamivir. There were significant reductions in the rates of all-cause ED visits (22% decline), hospitalizations (21% decline), and hospital length of stay (36% decline) across three combined influenza seasons. No significant differences were noted in respiratory-associated and all-cause deaths between intervention and control sites. This feasible, and low-cost intervention may provide significant benefit and should be further tested in other settings.PMID:36944078 | ...
Source: Annals of Family Medicine - March 21, 2023 Category: Primary Care Authors: Jonathan Temte Mary Checovich Marlon Mundt Shari Barlow Irene Hamrick Erik Reisdorf Source Type: research
Rapid Detection of Influenza Outbreaks in Long Term Care Facilities Reduces Emergency Room Visits and Hospitalization
Conclusions: The use of low threshold criteria to trigger nursing staff-initiated testing for influenza with RIDT resulted in increased prophylactic use of oseltamivir. There were significant reductions in the rates of all-cause ED visits (22% decline), hospitalizations (21% decline), and hospital length of stay (36% decline) across three combined influenza seasons. No significant differences were noted in respiratory-associated and all-cause deaths between intervention and control sites. This feasible, and low-cost intervention may provide significant benefit and should be further tested in other settings.PMID:36944078 | ...
Source: Annals of Family Medicine - March 21, 2023 Category: Primary Care Authors: Jonathan Temte Mary Checovich Marlon Mundt Shari Barlow Irene Hamrick Erik Reisdorf Source Type: research
Discovery of N-substituted oseltamivir derivatives as novel neuraminidase inhibitors with improved drug resistance profiles and favorable drug-like properties
Eur J Med Chem. 2023 Mar 13;252:115275. doi: 10.1016/j.ejmech.2023.115275. Online ahead of print.ABSTRACTTo yield potent neuraminidase inhibitors with improved drug resistance and favorable drug-like properties, two series of novel oseltamivir derivatives targeting the 150-cavity of neuraminidase were designed, synthesized, and biologically evaluated. Among the synthesized compounds, the most potent compound 43b bearing 3-floro-4-cyclopentenylphenzyl moiety exhibited weaker or slightly improved inhibitory activity against wild-type neuraminidases (NAs) of H1N1, H5N1, and H5N8 compared to oseltamivir carboxylate (OSC). Enco...
Source: European Journal of Medicinal Chemistry - March 17, 2023 Category: Chemistry Authors: Ruifang Jia Jiwei Zhang Fangyuan Shi Anna Bonomini Camilla Lucca Chiara Bertagnin Jian Zhang Chuanfeng Liu Huinan Jia Yuanmin Jiang Xiuli Ma Arianna Loregian Bing Huang Peng Zhan Xinyong Liu Source Type: research
Evolutionary dynamics of influenza A/H1N1 virus circulating in India from 2011 to 2021
Infect Genet Evol. 2023 Mar 11:105424. doi: 10.1016/j.meegid.2023.105424. Online ahead of print.ABSTRACTInfluenza A viruses (IAV) are fast-evolving pathogens with a very high mutation rate (2.0 × 10-6 to 2.0 × 10-4) compared to the influenza B (IBV) and influenza C (ICV) viruses. Generally, the tropical regions are considered as the reservoir for the IAV's genetic and antigenic evolutionary modification to be reintroduced into the temperate region. Therefore, in connection to the above facts, the present study emphasized on the evolutionary dynamic of the pandemic-2009 H1N1 (pdmH1N1) influenza virus in India. A total of ...
Source: Infection, Genetics and Evolution - March 13, 2023 Category: Genetics & Stem Cells Authors: Uttaran Bhattacharjee Alok Kumar Chakrabarti Suman Kanungo Shanta Dutta Source Type: research
Evolutionary dynamics of influenza A/H1N1 virus circulating in India from 2011 to 2021
Infect Genet Evol. 2023 Mar 11:105424. doi: 10.1016/j.meegid.2023.105424. Online ahead of print.ABSTRACTInfluenza A viruses (IAV) are fast-evolving pathogens with a very high mutation rate (2.0 × 10-6 to 2.0 × 10-4) compared to the influenza B (IBV) and influenza C (ICV) viruses. Generally, the tropical regions are considered as the reservoir for the IAV's genetic and antigenic evolutionary modification to be reintroduced into the temperate region. Therefore, in connection to the above facts, the present study emphasized on the evolutionary dynamic of the pandemic-2009 H1N1 (pdmH1N1) influenza virus in India. A total of ...
Source: Infection, Genetics and Evolution - March 13, 2023 Category: Genetics & Stem Cells Authors: Uttaran Bhattacharjee Alok Kumar Chakrabarti Suman Kanungo Shanta Dutta Source Type: research
Evolutionary dynamics of influenza A/H1N1 virus circulating in India from 2011 to 2021
Infect Genet Evol. 2023 Mar 11:105424. doi: 10.1016/j.meegid.2023.105424. Online ahead of print.ABSTRACTInfluenza A viruses (IAV) are fast-evolving pathogens with a very high mutation rate (2.0 × 10-6 to 2.0 × 10-4) compared to the influenza B (IBV) and influenza C (ICV) viruses. Generally, the tropical regions are considered as the reservoir for the IAV's genetic and antigenic evolutionary modification to be reintroduced into the temperate region. Therefore, in connection to the above facts, the present study emphasized on the evolutionary dynamic of the pandemic-2009 H1N1 (pdmH1N1) influenza virus in India. A total of ...
Source: Infection, Genetics and Evolution - March 13, 2023 Category: Genetics & Stem Cells Authors: Uttaran Bhattacharjee Alok Kumar Chakrabarti Suman Kanungo Shanta Dutta Source Type: research
Evolutionary dynamics of influenza A/H1N1 virus circulating in India from 2011 to 2021
Infect Genet Evol. 2023 Mar 11;110:105424. doi: 10.1016/j.meegid.2023.105424. Online ahead of print.ABSTRACTInfluenza A viruses (IAV) are fast-evolving pathogens with a very high mutation rate (2.0 × 10-6 to 2.0 × 10-4) compared to the influenza B (IBV) and influenza C (ICV) viruses. Generally, the tropical regions are considered as the reservoir for the IAV's genetic and antigenic evolutionary modification to be reintroduced into the temperate region. Therefore, in connection to the above facts, the present study emphasized on the evolutionary dynamic of the pandemic-2009 H1N1 (pdmH1N1) influenza virus in India. A total...
Source: Infection, Genetics and Evolution - March 13, 2023 Category: Genetics & Stem Cells Authors: Uttaran Bhattacharjee Alok Kumar Chakrabarti Suman Kanungo Shanta Dutta Source Type: research
Evolutionary dynamics of influenza A/H1N1 virus circulating in India from 2011 to 2021
Infect Genet Evol. 2023 Mar 11;110:105424. doi: 10.1016/j.meegid.2023.105424. Online ahead of print.ABSTRACTInfluenza A viruses (IAV) are fast-evolving pathogens with a very high mutation rate (2.0 × 10-6 to 2.0 × 10-4) compared to the influenza B (IBV) and influenza C (ICV) viruses. Generally, the tropical regions are considered as the reservoir for the IAV's genetic and antigenic evolutionary modification to be reintroduced into the temperate region. Therefore, in connection to the above facts, the present study emphasized on the evolutionary dynamic of the pandemic-2009 H1N1 (pdmH1N1) influenza virus in India. A total...
Source: Infection, Genetics and Evolution - March 13, 2023 Category: Genetics & Stem Cells Authors: Uttaran Bhattacharjee Alok Kumar Chakrabarti Suman Kanungo Shanta Dutta Source Type: research
Oseltamivir phosphate for suspension is bioequivalent to TAMIFLU in healthy volunteers: a randomized, open-label clinical study
CONCLUSION: Two Oseltamivir phosphate for suspensions are safe and bioequivalent.PMID:36810140 | PMC:PMC9942619 | DOI:10.1186/s40360-023-00646-1 (Source: BMC Pharmacology and Toxicology)
Source: BMC Pharmacology and Toxicology - February 22, 2023 Category: Drugs & Pharmacology Authors: Ying Wang Bangzhong Tang Jing Xie Xiaoqin Wang Peng Ren Guangmei Wu Cuixia He Minhui Zhu Yue Su Jiaxiang Ding Yuanyuan Xu Ling Fan Qin Ge Yuzhou Ding Juan Zhu Bingyan Liu Rongfang Shan Huan Zhou Source Type: research
