Hydrophobic substrate binding pocket remodeling of echinocandin B deacylase based on multi-dimensional rational design
Int J Biol Macromol. 2024 Apr 11;267(Pt 1):131473. doi: 10.1016/j.ijbiomac.2024.131473. Online ahead of print.ABSTRACTActinoplanes utahensis deacylase (AAC)-catalyzed deacylation of echinocandin B (ECB) is a promising method for the synthesis of anidulafungin, the newest of the echinocandin antifungal agents. However, the low activity of AAC significantly limits its practical application. In this work, we have devised a multi-dimensional rational design strategy for AAC, conducting separate analyses on the substrate-binding pocket's volume, curvature, and length. Furthermore, we quantitatively analyzed substrate properties...
Source: International Journal of Biological Macromolecules - April 13, 2024 Category: Biochemistry Authors: Heng Tang Han-Yue Zhu Yin-Feng Huang Ze-Yu Wu Shu-Ping Zou Zhi-Qiang Liu Yu-Guo Zheng Source Type: research
Genes, Vol. 15, Pages 488: Combined Analysis of Untargeted Metabolomics and Transcriptomics Revealed Seed Germination and Seedling Establishment in Zelkova schneideriana
This study conducted metabolomic and transcriptomic analyses of seed germination and seedling establishment in Z. schneideriana. Regular expression of genes and metabolite levels has been observed in plant hormone signal transduction, starch and sucrose metabolism, linoleic acid metabolism, and phenylpropanoid biosynthesis. The reduction in abscisic acid during seed germination may lead to seed release from dormancy. After the seed is released from dormancy, the metabolic levels of auxin, cytokinins, brassinolide, and various sugars are elevated, and they are consumed in large quantities during the seedling establishment s...
Source: Genes - April 12, 2024 Category: Genetics & Stem Cells Authors: Fengxia Yan Tangmei Wei Chao Yang Yanbing Yang Zaiqi Luo Yunli Jiang Tags: Article Source Type: research
Efficiency of clustering methods and self-organizing maps of Adriatic sardines and anchovies regarding organochlorine and fatty acid burden
In this study, we compared traditional classification methods traditional principal component analysis (PCA) and Ward's clustering with an advanced self-organizing map (SOM) algorithm in determining distribution patterns of 24 organochlorines and 19 fatty acids in sardine and anchovy samples taken from the eastern Adriatic. The outcomes reveal the strengths and weaknesses of the three approaches (PCA, Ward's clustering, and SOM). However, it is evident that SOM has proven to be the most effective in offering detailed information and data visualization. Although sardines and anchovies exhibit similar distribution patterns f...
Source: Environmental Science and Pollution Research International - April 11, 2024 Category: Environmental Health Authors: Igor Du čić Snje žana Herceg Romanić Bosiljka Musta ć Gordana Menda š Jasna Đinović-Stojanović Aleksandar Popovi ć Gordana Jovanovi ć Source Type: research
Molecules, Vol. 29, Pages 1696: The Composition of Volatiles and the Role of Non-Traditional LOX on Target Metabolites in Virgin Olive Oil from Autochthonous Dalmatian Cultivars
This article contributes to the selection of the optimal harvest time for the production of VOOs with the desired aromatic properties and to the knowledge of the varietal characteristics of VOOs. (Source: Molecules)
Source: Molecules - April 9, 2024 Category: Chemistry Authors: Barbara Soldo Maja Juki ć Špika Igor Paskovi ć Elma Vuko Marija Poli ć Pasković Ivica Ljubenkov Tags: Article Source Type: research
Evaluating the renoprotective effects of omega-3-6-9 against cisplatin-induced nephrotoxicity in mice
This study explored the renoprotective potential of omega-3-6-9 when co-administered with cisplatin in a mice model. We divided adult mice into five groups: a control group received 0.5 ml of liquid paraffin; a cisplatin-only group; two groups were treated with low (50 mg/kg) and high (100 mg/kg) doses of omega-3-6-9 plus cisplatin; and a final group received vitamin E before cisplatin administration. The administration of omega-3-6-9 significantly decreased pro-inflammatory modulators and kidney function markers such as TNF-α, IL-1β, blood urea nitrogen, and creatinine, indicating potential renoprotective effects. Our r...
Source: Journal of Medicine and Life - April 8, 2024 Category: General Medicine Authors: Saja Kareem Abd Alhusen Ali Faris Hasan Source Type: research
Changes in the consumption of isoflavones, omega-6, and omega-3 fatty acids in women with metastatic breast cancer adopting a whole-food, plant-based diet: post-hoc analysis of nutrient intake data from an 8-week randomized controlled trial
CONCLUSION: Transitioning to a WFPB diet resulted in significantly increased isoflavone intake and decreased n-6:n-3 ratio in women with breast cancer.PMID:38577156 | PMC:PMC10991800 | DOI:10.3389/fnut.2024.1338392 (Source: Cancer Control)
Source: Cancer Control - April 5, 2024 Category: Cancer & Oncology Authors: Jean Lee Erin K Campbell Eva Culakova Lisa M Blanchard Nellie Wixom Luke J Peppone Thomas M Campbell Source Type: research
Molecules, Vol. 29, Pages 1633: Chemoenzymatic Synthesis of ABC-Type Enantiostructured Triacylglycerols by the Use of the p-Methoxybenzyl Protective Group
This report demonstrates the first asymmetric synthesis of enantiopure structured triacylglycerols (TAGs) of the ABC type presenting three non-identical fatty acids, two of which are unsaturated. The unsaturated fatty acids included monounsaturated oleic acid (C18:1 n-9) and polyunsaturated linoleic acid (C18:2 n-6). This was accomplished by a six-step chemoenzymatic approach starting from (R)- and (S)-solketals. The highly regioselective immobilized Candida antarctica lipase (CAL-B) played a crucial role in the regiocontrol of the synthesis. The synthesis also benefited from the use of the p-methoxybenzyl (PMB) ether prot...
Source: Molecules - April 5, 2024 Category: Chemistry Authors: Hafdis Haraldsdottir Haraldur G. Gudmundsson Kaisa M. Linderborg Baoru Yang Gudmundur G. Haraldsson Tags: Article Source Type: research
Inhibitory effect of natural compounds on quorum sensing system in Pseudomonas aeruginosa: a helpful promise for managing biofilm community
This article explores many facets of utilizing natural compounds to inhibit and eradicate P. aeruginosa biofilms. It also examines the techniques and protocols employed to enhance the effectiveness of these compounds. (Source: Frontiers in Pharmacology)
Source: Frontiers in Pharmacology - April 2, 2024 Category: Drugs & Pharmacology Source Type: research
Disrupted gut microecology after high-dose 131I therapy and radioprotective effects of arachidonic acid supplementation
ConclusionThese findings highlight that ARA, as a key metabolite, substantially contributes to radioprotection. Our study provides novel insights into the pivotal role that the microbiota-metabolite axis plays in radionuclide protection and offers effective biological targets for treating radiation-induced adverse effects. (Source: European Journal of Nuclear Medicine and Molecular Imaging)
Source: European Journal of Nuclear Medicine and Molecular Imaging - April 2, 2024 Category: Nuclear Medicine Source Type: research
