High-throughput nanoscale crystallization of dihydropyridine active pharmaceutical ingredients
Single-crystal X-ray diffraction analysis of small molecule active pharmaceutical ingredients is a key technique in the confirmation of molecular connectivity, including absolute stereochemistry, as well as the solid-state form. However, accessing single crystals suitable for X-ray diffraction analysis of an active pharmaceutical ingredient can be experimentally laborious, especially considering the potential for multiple solid-state forms (solvates, hydrates and polymorphs). In recent years, methods for the exploration of experimental crystallization space of small molecules have undergone a `step-change', resulting in ne...
Source: Acta Crystallographica Section B - December 21, 2023 Category: Chemistry Authors: Metherall, J.P. Corner, P.A. McCabe, J.F. Hall, M.J. Probert, M.R. Tags: dihydropyridines high-throughput crystallization single-crystal X-ray diffraction small molecule encapsulated nanodroplet crystallization research papers Source Type: research

Late-onset non-obstructive mesenteric ischemia (NOMI) resulting from delayed absorption of overdosed antihypertensive drugs: An autopsy case report
AbstractNon-obstructive mesenteric ischemia (NOMI) is caused by reduced blood flow to the intestines without physical occlusion in the mesenteric artery. Previous reports show that drug overdose occasionally induces late-onset NOMI; however, in most cases, the reason for the delayed onset is unclear. Here, we present an autopsy case of late-onset NOMI that was induced by a drug overdose. An 80-year-old man was admitted to the intensive care unit because of severe hypotension after an overdose of antihypertensive drugs. He received vasopressor therapy and continuous hemodiafiltration dialysis; however, gastrointestinal deco...
Source: Forensic Science, Medicine, and Pathology - December 19, 2023 Category: Forensic Medicine Source Type: research

Regulation of oxytocin-induced calcium transients and gene expression in engineered myometrial tissues by tissue architecture and matrix rigidity
In this study, we engineered healthy-like and fibrotic-like myometrium by culturing human myometrial smooth muscle cells on polyacrylamide hydrogels micropatterned with fibronectin to independently tune matrix rigidity and tissue alignment, respectively. We then evaluated calcium transients in response to oxytocin stimulation. Isotropic myometrial tissues on stiff substrates (representing fibrotic myometrium) had shorter calcium transients due to shorter decay time compared to aligned myometrial tissues on soft substrates (representing healthy myometrium). Calcium transients in aligned tissues had longer response times and...
Source: Physiological Research - December 18, 2023 Category: Physiology Authors: Antonina P Maxey Jaya M Travis Megan L McCain Source Type: research

Regulation of oxytocin-induced calcium transients and gene expression in engineered myometrial tissues by tissue architecture and matrix rigidity
In this study, we engineered healthy-like and fibrotic-like myometrium by culturing human myometrial smooth muscle cells on polyacrylamide hydrogels micropatterned with fibronectin to independently tune matrix rigidity and tissue alignment, respectively. We then evaluated calcium transients in response to oxytocin stimulation. Isotropic myometrial tissues on stiff substrates (representing fibrotic myometrium) had shorter calcium transients due to shorter decay time compared to aligned myometrial tissues on soft substrates (representing healthy myometrium). Calcium transients in aligned tissues had longer response times and...
Source: Cell Research - December 18, 2023 Category: Cytology Authors: Antonina P Maxey Jaya M Travis Megan L McCain Source Type: research

Regulation of oxytocin-induced calcium transients and gene expression in engineered myometrial tissues by tissue architecture and matrix rigidity
In this study, we engineered healthy-like and fibrotic-like myometrium by culturing human myometrial smooth muscle cells on polyacrylamide hydrogels micropatterned with fibronectin to independently tune matrix rigidity and tissue alignment, respectively. We then evaluated calcium transients in response to oxytocin stimulation. Isotropic myometrial tissues on stiff substrates (representing fibrotic myometrium) had shorter calcium transients due to shorter decay time compared to aligned myometrial tissues on soft substrates (representing healthy myometrium). Calcium transients in aligned tissues had longer response times and...
Source: Physiological Research - December 18, 2023 Category: Physiology Authors: Antonina P Maxey Jaya M Travis Megan L McCain Source Type: research

Regulation of oxytocin-induced calcium transients and gene expression in engineered myometrial tissues by tissue architecture and matrix rigidity
In this study, we engineered healthy-like and fibrotic-like myometrium by culturing human myometrial smooth muscle cells on polyacrylamide hydrogels micropatterned with fibronectin to independently tune matrix rigidity and tissue alignment, respectively. We then evaluated calcium transients in response to oxytocin stimulation. Isotropic myometrial tissues on stiff substrates (representing fibrotic myometrium) had shorter calcium transients due to shorter decay time compared to aligned myometrial tissues on soft substrates (representing healthy myometrium). Calcium transients in aligned tissues had longer response times and...
Source: Physiological Research - December 18, 2023 Category: Physiology Authors: Antonina P Maxey Jaya M Travis Megan L McCain Source Type: research

Multi targets of cannabidiol (CBD) on skeletal mammalian and avian neuromuscular preparations
Nat Prod Res. 2023 Dec 6:1-10. doi: 10.1080/14786419.2023.2290675. Online ahead of print.ABSTRACTCannabidiol (CBD) has been used in diseases that affect the central nervous system. Its effects on the peripheral synapses are of great interest, since endocannabinoid receptors are expressed in muscles. CBD (0.3 mM) was analysed using mammalian and avian neuromuscular preparations, through myographic techniques in complementary protocols. Mammalian cells were examined by light microscopy while exogenous acetylcholine (40 µM) and potassium chloride (100 mM) were added into avian preparations, before and at the end of experimen...
Source: Natural Product Research - December 6, 2023 Category: Biochemistry Authors: Maria Luiza Rodrigues de Castro-da-Silva Anna Paula Farias-de-Fran ça Isabella Ravazoli Karollyna Corr êa Oliveira Val éria de Campos Orsi Edson Hideaki Yoshida Renata Vasques da Silva Tavares Yoko Oshima-Franco Source Type: research

Multi targets of cannabidiol (CBD) on skeletal mammalian and avian neuromuscular preparations
Nat Prod Res. 2023 Dec 6:1-10. doi: 10.1080/14786419.2023.2290675. Online ahead of print.ABSTRACTCannabidiol (CBD) has been used in diseases that affect the central nervous system. Its effects on the peripheral synapses are of great interest, since endocannabinoid receptors are expressed in muscles. CBD (0.3 mM) was analysed using mammalian and avian neuromuscular preparations, through myographic techniques in complementary protocols. Mammalian cells were examined by light microscopy while exogenous acetylcholine (40 µM) and potassium chloride (100 mM) were added into avian preparations, before and at the end of experimen...
Source: Natural Product Research - December 6, 2023 Category: Biochemistry Authors: Maria Luiza Rodrigues de Castro-da-Silva Anna Paula Farias-de-Fran ça Isabella Ravazoli Karollyna Corr êa Oliveira Val éria de Campos Orsi Edson Hideaki Yoshida Renata Vasques da Silva Tavares Yoko Oshima-Franco Source Type: research

PBPK/PD Modeling of Nifedipine for Precision Medicine in Pregnant Women: Enhancing Clinical Decision-Making for Optimal Drug Therapy
ConclusionBased on available literature data, the PBPK/PD model of Nifedipine in pregnancy demonstrated good predictive performance. It will help optimize individualized dosing of Nifedipine, improve treatment outcomes, and minimize the risk of adverse reactions in mothers and infants. (Source: Pharmaceutical Research)
Source: Pharmaceutical Research - December 4, 2023 Category: Drugs & Pharmacology Source Type: research

Slow on the Draw —ED Management of Elevated BP in Older Adults
AbstractPurpose of ReviewThe purpose of this study is to review data surrounding the emergency department management of elevated blood pressure in older adults, including the management of hypertensive crisis and  outpatient management of markedly elevated blood pressure.Recent FindingsAcute lowering of blood pressure in older adults with markedly elevated blood pressure may lead to serious complications without improvements in hospital length of stay, return visits, or mortality. Older adults presenting with elevated blood pressures without evidence of end-organ damage should be referred for outpatient management of thei...
Source: Current Hypertension Reports - November 21, 2023 Category: Primary Care Source Type: research