Intracardiac Thrombus in Sinus Rhythm: A Case of Missed Cardiac Amyloidosis
We describe a case of a 59 year-old female who presented to the hospital with dyspnea on exertion, orthopnea, pedal edema, and weight gain. She had a one year history of heart failure with preserved ejection fraction, treated with metoprolol, bumetanide, and metolazone, and three month history of AL amyloidosis, treated with Bortezomib and Dexamethasone. (Source: Journal of Cardiac Failure)
Source: Journal of Cardiac Failure - October 1, 2020 Category: Cardiology Authors: Jennifer DeSalvo, Benjamin Buck, Ajay Vallakati Tags: 314 Source Type: research

A Systematic Review and Meta ‐Analysis of Metolazone Compared to Chlorothiazide for Treatment of Acute Decompensated Heart Failure
Treatment of volume overload in the setting of acute decompensated heart failure (ADHF) is typically achieved through the use of loop diuretics. While they are highly effective, some patients may develop loop diuretic resistance. One strategy to overcome this scenario includes sequential nephron blockade with a thiazide ‐type diuretic; however, it is unknown which thiazide‐type diuretic used in this setting is most effective. A systematic review and meta‐analysis were performed to compare the efficacy and safety of chlorothiazide with metolazone as add‐on therapy in the setting of loop diuretic resistance f or the ...
Source: Pharmacotherapy: The Journal of Human Pharmacology and Drug Therapy - September 14, 2020 Category: Drugs & Pharmacology Authors: Taylor D. Steuber, Kristin M. Janzen, Meredith L. Howard Tags: Review Of Therapeutics Source Type: research

A Systematic Review and Meta ‐Analysis of Metolazone Compared to Chlorothiazide for Treatment of Acute Decompensated Heart Failure
ConclusionMetolazone is as effective as chlorothiazide as add ‐on to loop diuretics in treating ADHF without an increase in safety concerns. (Source: Pharmacotherapy: The Journal of Human Pharmacology and Drug Therapy)
Source: Pharmacotherapy: The Journal of Human Pharmacology and Drug Therapy - July 8, 2020 Category: Drugs & Pharmacology Authors: Taylor D. Steuber, Kristin M. Janzen, Meredith L. Howard Tags: REVIEW OF THERAPEUTICS Source Type: research

Diuretic Strategies for Loop Diuretic Resistance in Acute Heart Failure: The 3T Trial
This study compared combination diuretic strategies in acute heart failure (AHF) complicated by diuretic resistance (DR).BackgroundCombination diuretic regimens to overcome loop DR are commonly used but with limited evidence.MethodsThis study was a randomized, double-blinded trial in 60 patients hospitalized with AHF and intravenous (IV) loop DR. Patients were randomized to oral metolazone, IV chlorothiazide, or tolvaptan therapy. All patients received concomitant high-dose IV infusions of furosemide. The primary outcome was 48-h weight loss.ResultsThe cohort exhibited DR prior to enrollment, producing 1,188 ± 476&n...
Source: JACC: Heart Failure - December 12, 2019 Category: Cardiology Source Type: research

The Myth of Metolazone-Does Dose Timing with Loop Diuretics Matter?
This study assessed the frequency and timing of dosing of metolazone for differences in effectiveness and safety in combination with loop diuretic therapy in patients with acute decompensated heart failure (ADHF). (Source: Journal of Cardiac Failure)
Source: Journal of Cardiac Failure - August 1, 2019 Category: Cardiology Authors: Lindsay M. Carter, Janna C. Beavers, Stuart D. Russell Tags: 359 Source Type: research

Molecules, Vol. 24, Pages 2731: Supersaturation Potential of Amorphous Active Pharmaceutical Ingredients after Long-Term Storage
This study explores the effect of physical aging and/or crystallization on the supersaturation potential and crystallization kinetics of amorphous active pharmaceutical ingredients (APIs). Spray-dried, fully amorphous indapamide, metolazone, glibenclamide, hydrocortisone, hydrochlorothiazide, ketoconazole, and sulfathiazole were used as model APIs. The parameters used to assess the supersaturation potential and crystallization kinetics were the maximum supersaturation concentration (Cmax,app), the area under the curve (AUC), and the crystallization rate constant (k). These were compared for freshly spray-dried and aged/cry...
Source: Molecules - July 27, 2019 Category: Chemistry Authors: Khadijah Edueng Denny Mahlin Johan Gr åsjö Olivia Nylander Manish Thakrani Christel A.S. Bergstr öm Tags: Article Source Type: research

Tamm-Horsfall Protein/Uromodulin Deficiency Elicits Tubular Compensatory Responses Leading to Hypertension and Hyperuricemia.
Abstract Expression of Tamm-Horsfall protein (THP or uromodulin) is highly restricted to the kidneys' thick ascending limb (TAL) of loop of Henle. Despite the unique location and recent association of THP gene mutations with hereditary uromodulin-associated kidney disease and THP single nucleotide polymorphisms with chronic kidney disease and hypertension, the physiological function(s) of THP and its pathological involvement remain incompletely understood. By studying age-dependent changes of THP knockout (KO) mice, we show here that young KO mice had significant salt and water wasting but were partially responsiv...
Source: American Journal of Physiology. Renal Physiology - January 10, 2018 Category: Physiology Authors: Liu Y, Goldfarb D, El-Achkar TM, Lieske JC, Wu XR Tags: Am J Physiol Renal Physiol Source Type: research

Pharmacokinetic study of single- and multiple-dosing with metolazone tablets in healthy Chinese population.
CONCLUSIONS: Single dosing with 0.5 mg, 1 mg, or 2 mg metolazone yielded linear plasma pharmacokinetic properties in healthy Chinese subjects. Multiple oral doses of metolazone did not display significantly different distributions or elimination characteristics from those observed for a single dose. Gender factors did not appear to influence the pharmacokinetic parameter variation of metolazone. The tmax of metolazone increased in the fed condition. Metolazone was well tolerated at the tested dose in this study. TRIAL REGISTRATION: This investigation is retrospectively registered at chictr.org.cn (ChiCTR...
Source: BMC Pharmacology and Toxicology - November 19, 2017 Category: Drugs & Pharmacology Tags: BMC Pharmacol Toxicol Source Type: research

Pharmacokinetic study of single- and multiple-dosing with metolazone tablets in healthy Chinese population
Metolazone is a diuretic, saluretic and antihypertensive chemical compound from the quinazoline category that possesses medicinal features similar to those of other thiazide diuretic drugs. However, the pharma... (Source: BMC Clinical Pharmacology)
Source: BMC Clinical Pharmacology - November 16, 2017 Category: Drugs & Pharmacology Authors: Xueqing Li, Rutao Wang, Yang Liu, Yun Liu, Heng Zheng, Yabo Feng, Na Zhao, Hongbin Geng, Wanzhi Zhang and Aidong Wen Tags: Research article Source Type: research

Association of QT-Prolonging Medication Use in CKD with Electrocardiographic Manifestations.
CONCLUSIONS: Use of medications associated with QT prolongation is common in CKD; the safety implications of these findings should be considered in these high-risk patients. PODCAST: This article contains a podcast at https://www.asn-online.org/media/podcast/CJASN/2017_08_09_CJASNPodcast_17_09_b.mp3. PMID: 28793999 [PubMed - as supplied by publisher] (Source: Clinical Journal of the American Society of Nephrology : CJASN)
Source: Clinical Journal of the American Society of Nephrology : CJASN - August 9, 2017 Category: Urology & Nephrology Authors: Snitker S, Doerfler RM, Soliman EZ, Deo R, St Peter WL, Kramlik S, Fischer MJ, Navaneethan S, Delafontaine P, Jaar BG, Ojo A, Makos GK, Slaven A, Weir MR, Zhan M, Fink JC, for CRIC Study Investigators Tags: Clin J Am Soc Nephrol Source Type: research

The Increased Mortality Risk Associated with Metolazone in Acute Heart Failure is Mediated by Worsening Renal Function and Electrolyte Disturbances
Background: Current guidelines recommend the addition of a thiazide when diuresis is inadequate during acute decompensated HF (ADHF) treatment; however this is based on limited data. Given that dual segmental nephron blockade may have harmful effects, we set out to determine the relationship between metolazone use during ADHF and mortality utilizing propensity score adjustment. Methods: Patients admitted to 3 hospitals employing a common EMR with a discharge diagnosis of HF and who received IV diuretics within the first 7 days were studied. (Source: Journal of Cardiac Failure)
Source: Journal of Cardiac Failure - August 1, 2017 Category: Cardiology Authors: Meredith A. Brisco-Bacik, Jozine M. ter Maaten, Natasha A. Vedage, F. Perry Wilson, Jeffrey M. Testani Source Type: research

Oral Metolazone Increases Urine Output Comparable to Chlorothiazide IV as an Adjunct to Loop Diuretics in HFrEF Patients with ADHF
Thiazide diuretics are often utilized to overcome loop diuretic resistance when treating ADHF. In addition to a large cost advantage, several pharmacokinetic advantages exist when administering oral metolazone (MT) compared to IV chlorothiazide (CT); yet many providers are reluctant to utilize an oral formulation to treat ADHF. Several studies have found no difference in efficacy between the agents, however these studies had limited patients, utilized net UOP which can be misleading if patients are intubated or fluid restricted, and included HFpEF patients which may not have comparable UOP goals to HFrEF patients. (Source:...
Source: Journal of Cardiac Failure - August 1, 2017 Category: Cardiology Authors: Jerrica E. Shuster, Brian Bohn, Rim Hadgu, Hannah Pope Source Type: research

Combined aquaretic and diuretic therapy in acute heart failure.
CONCLUSION: Our strategy of treating AHF by achieving high UOP, while maintaining stable electrolytes and creatinine in a short period to euvolemic state, is safe. PMID: 28652798 [PubMed - in process] (Source: International Journal of Nephrology and Renovascular Disease)
Source: International Journal of Nephrology and Renovascular Disease - June 29, 2017 Category: Urology & Nephrology Tags: Int J Nephrol Renovasc Dis Source Type: research

Intravenous Chlorothiazide Versus Enteral Metolazone to Augment Loop Diuretic Therapy in the Intensive Care Unit.
Abstract BACKGROUND: In cases of loop diuretic resistance in the intensive care unit (ICU), recommendations for a specific second-line thiazide agent are lacking. OBJECTIVE: To compare the effects of intravenous chlorothiazide (CTZ) and enteral metolazone (MET) on urine output (UOP) when added to furosemide monotherapy therapy in critically ill adults. METHODS: This was a retrospective cohort study conducted in the medical, surgical, and cardiothoracic ICUs of a quaternary medical center. The primary outcome was change in UOP induced by the study interventions compared with furosemide alone. Secondary ou...
Source: The Annals of Pharmacotherapy - February 24, 2017 Category: Drugs & Pharmacology Authors: Michaud CJ, Mintus KC Tags: Ann Pharmacother Source Type: research

Efficacy and Safety of Intravenous Chlorothiazide versus Oral Metolazone in Patients with Acute Decompensated Heart Failure and Loop Diuretic Resistance
ConclusionOral metolazone was noninferior to intravenous chlorothiazide for enhancing net urine output in patients with ADHF and loop diuretic resistance and was similarly safe with regard to renal function and electrolyte abnormalities. Given the significant cost disparity between the two agents, these findings suggest that oral metolazone may be considered a first‐line option in this patient population.This article is protected by copyright. All rights reserved. (Source: Pharmacotherapy: The Journal of Human Pharmacology and Drug Therapy)
Source: Pharmacotherapy: The Journal of Human Pharmacology and Drug Therapy - July 9, 2016 Category: Drugs & Pharmacology Authors: Christine E. Shulenberger, Anthony Jiang, Sandeep Devabhakthuni, Vijay Ivaturi, Tao Liu, Brent N. Reed Tags: Original Research Article Source Type: research

31. Differential effects of intravenous bolus furosemide and continuous furosemide infusion on in-hospital management and outcomes among patients admitted with acute decompensated heart failure
Publication date: July 2016 Source:Journal of the Saudi Heart Association, Volume 28, Issue 3 Author(s): N. Alsuhebany, R. Aqel, H. Alballa, F. Al-Ayoubi, G. Bawazeer, K. Alhabib, H. AlFaleh, A. Hersi, A. Ullah, T. Kashour Loop diuretics are a cornerstone in the management of Acute Decompensated Heart Failure (ADHF). However, the best therapeutic strategy in terms of intermittent boluses versus continuous infusion is still unclear.We set to examine the differences in hospital management and short-term and long-term mortality of patient receiving furosemide bolus or infusion treatment for ADHF.This is a retrospe...
Source: Journal of the Saudi Heart Association - June 11, 2016 Category: Cardiology Source Type: research

Efficacy of Intravenous Chlorothiazide for Refractory Acute Decompensated Heart Failure Unresponsive to Adjunct Metolazone
ConclusionThe addition of intravenous chlorothiazide did not result in improved diuresis in patients with ADHF determined to be refractory to loop diuretics and adjunctive oral metolazone.This article is protected by copyright. All rights reserved. (Source: Pharmacotherapy: The Journal of Human Pharmacology and Drug Therapy)
Source: Pharmacotherapy: The Journal of Human Pharmacology and Drug Therapy - May 1, 2016 Category: Drugs & Pharmacology Authors: Maria Cardinale, Jerry Altshuler, Jeffrey M. Testani Tags: Original Research Article Source Type: research

Preparation of a New Chiral Stationary Phase Based on Macrocyclic Amide Chiral Selector for the Liquid Chromatographic Chiral Separations.
Abstract A new chiral stationary phase (CSP) based on macrocyclic amide receptor was prepared starting from (1R,2R)-1,2-diphenylethylenediamine. The new CSP was successfully applied to the resolution of various N-(substituted benzoyl)-α-amino amides with reasonably good separation factors and resolutions (α = 1.75 ~ 2.97 and RS  = 2.89 ~ 6.82 for 16 analytes). The new CSP was also applied to the resolution of 3-substituted 1,4-benzodiazepin-2-ones and some diuretic chiral drugs including bendroflumethiazide and methylchlothiazide and metolazone. T...
Source: Chirality - January 4, 2016 Category: Molecular Biology Authors: Sung JY, Choi SH, Hyun MH Tags: Chirality Source Type: research

Predicting outcomes among patients with atrial fibrillation and heart failure receiving anticoagulation with warfarin.
Abstract Among patients receiving oral anticoagulation for atrial fibrillation (AF), heart failure (HF) is associated with poor anticoagulation control. However, it is not known which patients with heart failure are at greatest risk of adverse outcomes. We evaluated 62,156 Veterans Health Administration (VA) patients receiving warfarin for AF between 10/1/06-9/30/08 using merged VA-Medicare dataset. We predicted time in therapeutic range (TTR) and rates of adverse events by categorising patients into those with 0, 1, 2, or 3+ of five putative markers of HF severity such as aspartate aminotransferase (AST)>&thin...
Source: Thrombosis and Haemostasis - May 7, 2015 Category: Hematology Authors: Kim EJ, Ozonoff A, Hylek EM, Berlowitz DR, Ash AS, Miller DR, Zhao S, Reisman JI, Jasuja GK, Rose AJ Tags: Thromb Haemost Source Type: research

Thiazide Diuretics in Chronic Kidney Disease
Abstract Widely prevalent in the general population, chronic kidney disease (CKD) is frequently complicated with hypertension. Control of hypertension in this high-risk population is a major modifiable cardiovascular and renal risk factor but often requires multiple medications. Although thiazides are an attractive agent, guidelines have previously recommended against thiazide use in stage 4 CKD. We review the updated guidelines on thiazide use in advanced CKD, the antihypertensive mechanism of thiazides, and the clinical studies of thiazides in CKD. Older uncontrolled studies have shown that metolazone reduces b...
Source: Current Hypertension Reports - March 7, 2015 Category: Primary Care Source Type: research

Comparison of metolazone versus chlorothiazide in acute decompensated heart failure with diuretic resistance
ConclusionSequential nephron blockade with either metolazone or chlorothiazide appears to be efficacious and safe in ADHF, renal dysfunction, and diuretic resistance. Given the considerable cost difference favoring oral metolazone, larger randomized studies are warranted to confirm our findings and to exclude the possibility of confounding by indication.This article is protected by copyright. All rights reserved. (Source: Cardiovascular Therapeutics)
Source: Cardiovascular Therapeutics - February 1, 2015 Category: Cardiology Authors: Michael Moranville, Suji Choi, Jennifer Hogg, Allen Anderson, Jonathan Rich Tags: Original Research Article Source Type: research

Hypotension During Hospitalization for Acute Heart Failure Is Independently Associated With 30-Day Mortality: Findings From ASCEND-HF [Original Articles]
Background— Outcomes associated with episodes of hypotension while hospitalized with acute decompensated heart failure are not well understood. Methods and Results— Using data from Acute Study of Clinical Effectiveness of Nesiritide in Decompensated Heart Failure (ASCEND-HF), we assessed factors associated with in-hospital hypotension and subsequent 30-day outcomes. Patients were classified as having symptomatic or asymptomatic hypotension. Multivariable logistic regression was used to determine factors associated with in-hospital hypotension, and Cox proportional hazards models were used to assess the associa...
Source: Circulation: Heart Failure - November 18, 2014 Category: Cardiology Authors: Patel, P. A., Heizer, G., O'Connor, C. M., Schulte, P. J., Dickstein, K., Ezekowitz, J. A., Armstrong, P. W., Hasselblad, V., Mills, R. M., McMurray, J. J. V., Starling, R. C., Tang, W. H. W., Califf, R. M., Hernandez, A. F. Tags: Other heart failure Original Articles Source Type: research

68 y.o. male with weakness: “Treat the monitor, not the patient?”
Discussion: The nearest 24/7 PCI center is 20 minutes away, while a smaller community hospital (fibrinolysis only, but has an ICU, etc.) is “just around the corner.” What is your transport destination? (Source: EMS 12-Lead)
Source: EMS 12-Lead - September 28, 2014 Category: Cardiology Authors: Brooks Walsh MD Tags: 12 lead ecg Uncategorized Source Type: research

Thiazide-like diuretic drug metolazone activates human pregnane X receptor to induce cytochrome 3A4 and multidrug-resistance protein 1.
Abstract Human pregnane X receptor (hPXR) regulates the expression of drug-metabolizing enzyme cytochrome P450 3A4 (CYP3A4) and drug transporters such as multidrug-resistance protein 1 (MDR1). PXR can be modulated by small molecules, including Federal Drug Administration (FDA)-approved drugs, thus altering drug metabolism and causing drug-drug interactions. To determine the role of FDA-approved drugs in PXR-mediated regulation of drug metabolism and clearance, we screened 1481 FDA-approved small-molecule drugs by using a luciferase reporter assay in HEK293T cells and identified the diuretic drug metolazone as an a...
Source: Biochemical Pharmacology - August 30, 2014 Category: Drugs & Pharmacology Authors: Banerjee M, Chen T Tags: Biochem Pharmacol Source Type: research

Blood pressure-lowering efficacy of monotherapy with thiazide diuretics for primary hypertension.
CONCLUSIONS: This systematic review shows that hydrochlorothiazide has a dose-related blood pressure-lowering effect. The mean blood pressure-lowering effect over the dose range 6.25 mg, 12.5 mg, 25 mg and 50 mg/day is 4/2 mmHg, 6/3 mmHg, 8/3 mmHg and 11/5 mmHg, respectively. For other thiazide drugs, the lowest doses studied lowered blood pressure maximally and higher doses did not lower it more. Due to the greater effect on systolic than on diastolic blood pressure, thiazides lower pulse pressure by 4 mmHg to 6 mmHg. This exceeds the mean 3 mmHg pulse pressure reduction achieved by ACE inhibitors, ARBs and renin inhibito...
Source: Cochrane Database of Systematic Reviews - June 3, 2014 Category: Journals (General) Authors: Musini VM, Nazer M, Bassett K, Wright JM Tags: Cochrane Database Syst Rev Source Type: research

Diuretic Exposure in Premature Infants from 1997 to 2011
We examined their use and safety in this group. Study Design Retrospective cohort study of infants 
Source: American Journal of Perinatology - May 6, 2014 Category: Perinatology & Neonatology Authors: Laughon, Matthew M.Chantala, KimAliaga, SofiaHerring, Amy H.Hornik, Christoph P.Hughes, RachelClark, Reese H.Smith, P Brian Tags: Original Article Source Type: research

Effect of intensified diuretic therapy on overnight rostral fluid shift and obstructive sleep apnoea in patients with uncontrolled hypertension
Objectives:Fluid displacement from the lower extremities to the upper body during sleep is strongly associated with obstructive sleep apnoea in hypertensive patients. The present pathophysiological study tests the hypothesis that intensified diuretic therapy will reduce the apnoea-hypopnoea index and blood pressure of uncontrolled hypertensive patients with obstructive sleep apnoea in proportion to the reduction in overnight change in leg fluid volume. Methods:Uncontrolled treated hypertensive patients underwent overnight polysomnography and measurement of overnight changes in leg fluid volume and neck circumference. Those...
Source: Journal of Hypertension - February 3, 2014 Category: Cardiology Tags: ORIGINAL PAPERS: Hypertension management Source Type: research

Comparison of Bumetanide- and Metolazone-Based Diuretic Regimens to Furosemide in Acute Heart Failure
Introduction: Limited data exist comparing the efficacy and safety of bumetanide- or metolazone-based diuretic regimens to furosemide in acute heart failure (HF). Our purpose was to evaluate the comparative effect on urine output (UO) and renal function between these regimens. Methods: A retrospective study of hospitalized HF patients treated with continuous infusion furosemide (CIF), combination furosemide plus metolazone (F + M), or continuous infusion bumetanide (CIB). Primary end points were between regimen comparisons for change in mean hourly UO versus baseline and incidence of worsening renal function. Results: ...
Source: Journal of Cardiovascular Pharmacology and Therapeutics - June 12, 2013 Category: Cardiology Authors: Ng, T. M. H., Konopka, E., Hyderi, A. F., Hshieh, S., Tsuji, Y., Kim, B. J., Han, S. Y., Phan, D. H., Jeng, A. I., Lou, M., Elkayam, U. Tags: Clinical Studies Source Type: research

Intravenous moderate-dose bumetanide continuous infusion and severe musculoskeletal pain
We present a rare adverse side effect of moderate-to-high dose bumetanide continuous infusion in a 72year old Hispanic woman with a history of three-vessel coronary artery disease (CAD), heart failure (HF) with reduced ejection fraction, type 2 diabetes and stage III chronic kidney disease. The patient had been hospitalized for dyspnea and volume retention in the setting of progressive acute on chronic renal failure with oliguria. Trials of high-dose (160–240mg) fractionated bolus doses of intravenous furosemide were attempted in the first 48 to 72h with minimal urine output (10–20cm3/h). Metolazone was added t...
Source: International Journal of Cardiology - June 3, 2013 Category: Cardiology Authors: Muthiah Vaduganathan, Andrew S. Allegretti, Anastasia M. Manchette, Shalin S. Patel, Kristian R. Olson, Hasan Bazari Tags: Online Letters to the editor Source Type: research

Abstract 25: Predicting Outcomes among Patients with Heart Failure Receiving Anticoagulation with Warfarin [Concurrent III Session A: Oral Abstract Presentations on EHS & Health IT]
We examined 66,099 subjects who had been anticoagulated for stroke prevention in AF for at least 6 months between 10/1/06-9/30/08. Based on ICD-9 codes, patients were divided between those who carried a diagnosis of CHF and those who did not. Among patients with CHF, we examined various predictors of percent time in therapeutic range (TTR), a measure of anticoagulation control. The predictors were chosen by the principle that more severe CHF would cause hepatic congestion, leading to a greater variability of anticoagulation control and greater risk of adverse events. We identified ten severity markers which predicted worse...
Source: Circulation: Cardiovascular Quality and Outcomes - May 15, 2013 Category: Cardiology Authors: Kim, E.-J., Rose, A. Tags: Concurrent III Session A: Oral Abstract Presentations on EHS & amp; Health IT Source Type: research

The tight junction protein claudin-b regulates epithelial permeability and sodium handling in larval zebrafish, Danio rerio
The functional role of the tight junction protein claudin-b in larval zebrafish (Danio rerio) was investigated. We showed that claudin-b protein is expressed at epithelial cell-cell contacts on the skin. Translational gene knockdown of claudin-b protein expression caused developmental defects, including edema in the pericardial cavity and yolk sac. Claudin-b morphants exhibited an increase in epithelial permeability to the paracellular marker polyethylene glycol (PEG-4000) and fluorescein isothiocyanate-dextran (FD-4). Accumulation of FD-4 was confined mainly to the yolk sac and pericardial cavity in the claudin-b morphant...
Source: AJP: Regulatory, Integrative and Comparative Physiology - April 1, 2013 Category: Physiology Authors: Kwong, R. W. M., Perry, S. F. Tags: Fluid and Electrolyte Homeostasis Source Type: research

The tight junction protein claudin-b regulates epithelial permeability and sodium handling in larval zebrafish, Danio rerio.
Abstract The functional role of the tight junction protein claudin-b in larval zebrafish (Danio rerio) was investigated. We showed that claudin-b protein is expressed at epithelial cell-cell contacts on the skin. Translational gene knockdown of claudin-b protein expression caused developmental defects, including edema in the pericardial cavity and yolk sac. Claudin-b morphants exhibited an increase in epithelial permeability to the paracellular marker polyethylene glycol (PEG-4000) and fluorescein isothiocyanate-dextran (FD-4). Accumulation of FD-4 was confined mainly to the yolk sac and pericardial cavity in the ...
Source: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology - January 30, 2013 Category: Physiology Authors: Kwong RW, Perry SF Tags: Am J Physiol Regul Integr Comp Physiol Source Type: research