Effect of MAOA rs1465108 polymorphism on susceptibility to substance/alcohol use disorder: a novel PCR-RFLP assay for the detection of MAOA rs1465108
CONCLUSIONS: The results presented here do not support the hypothesis that MAOA rs1465108 is associated with substance/alcohol use disorders. The intensity of depressive symptoms could be changed according to the abused substance type. A novel PCR-RFLP was developed for genotyping of MAOA rs1465108 polymorphism, which could be a better option for laboratories without high technology equipment.PMID:38457024 | DOI:10.1007/s11033-024-09366-z (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - March 8, 2024 Category: Molecular Biology Authors: Dilek Kaya-Aky üzlü Selin Özkan-Kotiloğlu Sariye Ayb üke Yıldırım Mustafa Dan ışman Mukaddes Asena Y ıldırım İnci Özgür-İlhan Source Type: research
Effect of MAOA rs1465108 polymorphism on susceptibility to substance/alcohol use disorder: a novel PCR-RFLP assay for the detection of MAOA rs1465108
CONCLUSIONS: The results presented here do not support the hypothesis that MAOA rs1465108 is associated with substance/alcohol use disorders. The intensity of depressive symptoms could be changed according to the abused substance type. A novel PCR-RFLP was developed for genotyping of MAOA rs1465108 polymorphism, which could be a better option for laboratories without high technology equipment.PMID:38457024 | DOI:10.1007/s11033-024-09366-z (Source: Molecular Biology Reports)
Source: Molecular Biology Reports - March 8, 2024 Category: Molecular Biology Authors: Dilek Kaya-Aky üzlü Selin Özkan-Kotiloğlu Sariye Ayb üke Yıldırım Mustafa Dan ışman Mukaddes Asena Y ıldırım İnci Özgür-İlhan Source Type: research
Evaluating the Protective Effects of Thymoquinone on Methamphetamine-induced Toxicity in an < em > In Vitro < /em > Model Based on Differentiated PC12 Cells
This study aimed to investigate the potential protective effects of TQ against Meth-induced toxicity by using an in vitro model based on nerve growth factor-differentiated PC12 cells. Cell differentiation was assessed by detecting the presence of a neuronal marker with flow cytometry. The effects of Meth exposure were evaluated in the in vitro neuronal cell-based model via the determination of cell viability (in an MTT assay) and apoptosis (by annexin/propidium iodide staining). The generation of reactive oxygen species (ROS), as well as the levels of glutathione (GSH) and dopamine, were also determined. The model was used...
Source: Alternatives to Laboratory Animals : ATLA - March 6, 2024 Category: Research Authors: Seyed Mobin Seyed Aliyan Ali Roohbakhsh Marzieh Jafari Fakhrabad Zahar Salmasi Mohammad Moshiri Niosha Shahbazi Leila Etemad Source Type: research
Evaluating the Protective Effects of Thymoquinone on Methamphetamine-induced Toxicity in an < em > In Vitro < /em > Model Based on Differentiated PC12 Cells
This study aimed to investigate the potential protective effects of TQ against Meth-induced toxicity by using an in vitro model based on nerve growth factor-differentiated PC12 cells. Cell differentiation was assessed by detecting the presence of a neuronal marker with flow cytometry. The effects of Meth exposure were evaluated in the in vitro neuronal cell-based model via the determination of cell viability (in an MTT assay) and apoptosis (by annexin/propidium iodide staining). The generation of reactive oxygen species (ROS), as well as the levels of glutathione (GSH) and dopamine, were also determined. The model was used...
Source: Alternatives to Laboratory Animals : ATLA - March 6, 2024 Category: Research Authors: Seyed Mobin Seyed Aliyan Ali Roohbakhsh Marzieh Jafari Fakhrabad Zahar Salmasi Mohammad Moshiri Niosha Shahbazi Leila Etemad Source Type: research
Evaluating the Protective Effects of Thymoquinone on Methamphetamine-induced Toxicity in an < em > In Vitro < /em > Model Based on Differentiated PC12 Cells
This study aimed to investigate the potential protective effects of TQ against Meth-induced toxicity by using an in vitro model based on nerve growth factor-differentiated PC12 cells. Cell differentiation was assessed by detecting the presence of a neuronal marker with flow cytometry. The effects of Meth exposure were evaluated in the in vitro neuronal cell-based model via the determination of cell viability (in an MTT assay) and apoptosis (by annexin/propidium iodide staining). The generation of reactive oxygen species (ROS), as well as the levels of glutathione (GSH) and dopamine, were also determined. The model was used...
Source: Alternatives to Laboratory Animals : ATLA - March 6, 2024 Category: Research Authors: Seyed Mobin Seyed Aliyan Ali Roohbakhsh Marzieh Jafari Fakhrabad Zahar Salmasi Mohammad Moshiri Niosha Shahbazi Leila Etemad Source Type: research
Evaluating the Protective Effects of Thymoquinone on Methamphetamine-induced Toxicity in an < em > In Vitro < /em > Model Based on Differentiated PC12 Cells
This study aimed to investigate the potential protective effects of TQ against Meth-induced toxicity by using an in vitro model based on nerve growth factor-differentiated PC12 cells. Cell differentiation was assessed by detecting the presence of a neuronal marker with flow cytometry. The effects of Meth exposure were evaluated in the in vitro neuronal cell-based model via the determination of cell viability (in an MTT assay) and apoptosis (by annexin/propidium iodide staining). The generation of reactive oxygen species (ROS), as well as the levels of glutathione (GSH) and dopamine, were also determined. The model was used...
Source: Alternatives to Laboratory Animals : ATLA - March 6, 2024 Category: Research Authors: Seyed Mobin Seyed Aliyan Ali Roohbakhsh Marzieh Jafari Fakhrabad Zahar Salmasi Mohammad Moshiri Niosha Shahbazi Leila Etemad Source Type: research
Evaluating the Protective Effects of Thymoquinone on Methamphetamine-induced Toxicity in an < em > In Vitro < /em > Model Based on Differentiated PC12 Cells
This study aimed to investigate the potential protective effects of TQ against Meth-induced toxicity by using an in vitro model based on nerve growth factor-differentiated PC12 cells. Cell differentiation was assessed by detecting the presence of a neuronal marker with flow cytometry. The effects of Meth exposure were evaluated in the in vitro neuronal cell-based model via the determination of cell viability (in an MTT assay) and apoptosis (by annexin/propidium iodide staining). The generation of reactive oxygen species (ROS), as well as the levels of glutathione (GSH) and dopamine, were also determined. The model was used...
Source: Alternatives to Laboratory Animals : ATLA - March 6, 2024 Category: Research Authors: Seyed Mobin Seyed Aliyan Ali Roohbakhsh Marzieh Jafari Fakhrabad Zahar Salmasi Mohammad Moshiri Niosha Shahbazi Leila Etemad Source Type: research
Evaluating the Protective Effects of Thymoquinone on Methamphetamine-induced Toxicity in an < em > In Vitro < /em > Model Based on Differentiated PC12 Cells
This study aimed to investigate the potential protective effects of TQ against Meth-induced toxicity by using an in vitro model based on nerve growth factor-differentiated PC12 cells. Cell differentiation was assessed by detecting the presence of a neuronal marker with flow cytometry. The effects of Meth exposure were evaluated in the in vitro neuronal cell-based model via the determination of cell viability (in an MTT assay) and apoptosis (by annexin/propidium iodide staining). The generation of reactive oxygen species (ROS), as well as the levels of glutathione (GSH) and dopamine, were also determined. The model was used...
Source: Alternatives to Laboratory Animals : ATLA - March 6, 2024 Category: Research Authors: Seyed Mobin Seyed Aliyan Ali Roohbakhsh Marzieh Jafari Fakhrabad Zahar Salmasi Mohammad Moshiri Niosha Shahbazi Leila Etemad Source Type: research
Evaluating the Protective Effects of Thymoquinone on Methamphetamine-induced Toxicity in an < em > In Vitro < /em > Model Based on Differentiated PC12 Cells
This study aimed to investigate the potential protective effects of TQ against Meth-induced toxicity by using an in vitro model based on nerve growth factor-differentiated PC12 cells. Cell differentiation was assessed by detecting the presence of a neuronal marker with flow cytometry. The effects of Meth exposure were evaluated in the in vitro neuronal cell-based model via the determination of cell viability (in an MTT assay) and apoptosis (by annexin/propidium iodide staining). The generation of reactive oxygen species (ROS), as well as the levels of glutathione (GSH) and dopamine, were also determined. The model was used...
Source: Alternatives to Laboratory Animals : ATLA - March 6, 2024 Category: Research Authors: Seyed Mobin Seyed Aliyan Ali Roohbakhsh Marzieh Jafari Fakhrabad Zahar Salmasi Mohammad Moshiri Niosha Shahbazi Leila Etemad Source Type: research
Evaluating the Protective Effects of Thymoquinone on Methamphetamine-induced Toxicity in an < em > In Vitro < /em > Model Based on Differentiated PC12 Cells
This study aimed to investigate the potential protective effects of TQ against Meth-induced toxicity by using an in vitro model based on nerve growth factor-differentiated PC12 cells. Cell differentiation was assessed by detecting the presence of a neuronal marker with flow cytometry. The effects of Meth exposure were evaluated in the in vitro neuronal cell-based model via the determination of cell viability (in an MTT assay) and apoptosis (by annexin/propidium iodide staining). The generation of reactive oxygen species (ROS), as well as the levels of glutathione (GSH) and dopamine, were also determined. The model was used...
Source: Alternatives to Laboratory Animals : ATLA - March 6, 2024 Category: Research Authors: Seyed Mobin Seyed Aliyan Ali Roohbakhsh Marzieh Jafari Fakhrabad Zahar Salmasi Mohammad Moshiri Niosha Shahbazi Leila Etemad Source Type: research
Evaluating the Protective Effects of Thymoquinone on Methamphetamine-induced Toxicity in an < em > In Vitro < /em > Model Based on Differentiated PC12 Cells
This study aimed to investigate the potential protective effects of TQ against Meth-induced toxicity by using an in vitro model based on nerve growth factor-differentiated PC12 cells. Cell differentiation was assessed by detecting the presence of a neuronal marker with flow cytometry. The effects of Meth exposure were evaluated in the in vitro neuronal cell-based model via the determination of cell viability (in an MTT assay) and apoptosis (by annexin/propidium iodide staining). The generation of reactive oxygen species (ROS), as well as the levels of glutathione (GSH) and dopamine, were also determined. The model was used...
Source: Alternatives to Laboratory Animals : ATLA - March 6, 2024 Category: Research Authors: Seyed Mobin Seyed Aliyan Ali Roohbakhsh Marzieh Jafari Fakhrabad Zahar Salmasi Mohammad Moshiri Niosha Shahbazi Leila Etemad Source Type: research
Evaluating the Protective Effects of Thymoquinone on Methamphetamine-induced Toxicity in an < em > In Vitro < /em > Model Based on Differentiated PC12 Cells
This study aimed to investigate the potential protective effects of TQ against Meth-induced toxicity by using an in vitro model based on nerve growth factor-differentiated PC12 cells. Cell differentiation was assessed by detecting the presence of a neuronal marker with flow cytometry. The effects of Meth exposure were evaluated in the in vitro neuronal cell-based model via the determination of cell viability (in an MTT assay) and apoptosis (by annexin/propidium iodide staining). The generation of reactive oxygen species (ROS), as well as the levels of glutathione (GSH) and dopamine, were also determined. The model was used...
Source: Alternatives to Laboratory Animals : ATLA - March 6, 2024 Category: Research Authors: Seyed Mobin Seyed Aliyan Ali Roohbakhsh Marzieh Jafari Fakhrabad Zahar Salmasi Mohammad Moshiri Niosha Shahbazi Leila Etemad Source Type: research
Evaluating the Protective Effects of Thymoquinone on Methamphetamine-induced Toxicity in an < em > In Vitro < /em > Model Based on Differentiated PC12 Cells
This study aimed to investigate the potential protective effects of TQ against Meth-induced toxicity by using an in vitro model based on nerve growth factor-differentiated PC12 cells. Cell differentiation was assessed by detecting the presence of a neuronal marker with flow cytometry. The effects of Meth exposure were evaluated in the in vitro neuronal cell-based model via the determination of cell viability (in an MTT assay) and apoptosis (by annexin/propidium iodide staining). The generation of reactive oxygen species (ROS), as well as the levels of glutathione (GSH) and dopamine, were also determined. The model was used...
Source: Alternatives to Laboratory Animals : ATLA - March 6, 2024 Category: Research Authors: Seyed Mobin Seyed Aliyan Ali Roohbakhsh Marzieh Jafari Fakhrabad Zahar Salmasi Mohammad Moshiri Niosha Shahbazi Leila Etemad Source Type: research
Evaluating the Protective Effects of Thymoquinone on Methamphetamine-induced Toxicity in an < em > In Vitro < /em > Model Based on Differentiated PC12 Cells
This study aimed to investigate the potential protective effects of TQ against Meth-induced toxicity by using an in vitro model based on nerve growth factor-differentiated PC12 cells. Cell differentiation was assessed by detecting the presence of a neuronal marker with flow cytometry. The effects of Meth exposure were evaluated in the in vitro neuronal cell-based model via the determination of cell viability (in an MTT assay) and apoptosis (by annexin/propidium iodide staining). The generation of reactive oxygen species (ROS), as well as the levels of glutathione (GSH) and dopamine, were also determined. The model was used...
Source: Alternatives to Laboratory Animals : ATLA - March 6, 2024 Category: Research Authors: Seyed Mobin Seyed Aliyan Ali Roohbakhsh Marzieh Jafari Fakhrabad Zahar Salmasi Mohammad Moshiri Niosha Shahbazi Leila Etemad Source Type: research
