Tomo Live: an on-the-fly reconstruction pipeline to judge data quality for cryo-electron tomography workflows
Data acquisition and processing for cryo-electron tomography can be a significant bottleneck for users. To simplify and streamline the cryo-ET workflow, Tomo Live, an on-the-fly solution that automates the alignment and reconstruction of tilt-series data, enabling real-time data-quality assessment, has been developed. Through the integration of Tomo Live into the data-acquisition workflow for cryo-ET, motion correction is performed directly after each of the acquired tilt angles. Immediately after the tilt-series acquisition has completed, an unattended tilt-series alignment and reconstruction into a 3D volume is performed...
Source: Acta Crystallographica Section D - March 21, 2024 Category: Biochemistry Authors: Comet, M. Dijkman, P.M. Boer Iwema, R. Franke, T. Masiulis, S. Schampers, R. Raschdorf, O. Grollios, F. Pryor, E.E. Drulyte, I. Tags: electron tomography real-time processing cryo-ET Tomo Live research papers Source Type: research
Cancers, Vol. 16, Pages 1242: Synthetic Circular RNA for microRNA-1269a Suppresses Tumor Progression in Oral Squamous Cell Carcinoma
In this study, we carried out cellular and luciferase reporter assays to examine competitive inhibition of miR-1269a, which is upregulated expression in several cancers, by circRNA-1269a, a synthetic circRNA that contains miR-1269a binding sequences. We also used data-independent acquisition (DIA) proteomics and in silico analyses to determine how circRNA-1269a treatment affects molecules downstream of miR-1269a. First, we confirmed the circularization of the linear miR-1269a binding site sequence using RT-PCR with divergent/convergent primers and direct sequencing of the head-to-tail circRNA junction point. In luciferase ...
Source: Cancers - March 21, 2024 Category: Cancer & Oncology Authors: Atsushi Kasamatsu Ryunosuke Nozaki Kohei Kawasaki Tomoaki Saito Chikashi Minemura Naohiko Seki Joel Moss Katsuhiro Uzawa Tags: Article Source Type: research
Whole-Body Magnetic Resonance Imaging in Rheumatology
This review focuses on the most frequent whole-body MRI applications in patients with rheumatological pathologies, for which this tool can be helpful to both radiologists and clinicians. It reports technical aspects of the acquisition of both 1.5 and 3.0 T scanners. The article lists the main findings that help radiologists during the evaluation of a specific pathology, both in the diagnostic phase and during follow-up. (Source: Radiologic Clinics of North America)
Source: Radiologic Clinics of North America - March 21, 2024 Category: Radiology Authors: Vito Chianca, Micha ł Lanckoroński, Marco Curti, Majid Chalian, Iwona Sudoł-Szopińska, Chiara Giraudo, Filippo Del Grande Source Type: research
Molecular aspects of cervical cancer: a pathogenesis update
Cervical cancer (CC) is a significant health problem, especially in low-income countries. Functional studies on the human papillomavirus have generated essential advances in the knowledge of CC. However, many unanswered questions remain. This mini-review discusses the latest results on CC pathogenesis, HPV oncogenesis, and molecular changes identified through next-generation technologies. Interestingly, the percentage of samples with HPV genome integrations correlates with the degree of the cervical lesions, suggesting a role in the development of CC. Also, new functions have been described for the viral oncoproteins E5, E...
Source: Frontiers in Oncology - March 19, 2024 Category: Cancer & Oncology Source Type: research
Deciphering cellular and molecular mechanism of MUC13 mucin involved in cancer cell plasticity and drug resistance
AbstractThere has been a surge of interest in recent years in understanding the intricate mechanisms underlying cancer progression and treatment resistance. One molecule that has recently emerged in these mechanisms is MUC13 mucin, a transmembrane glycoprotein. Researchers have begun to unravel the molecular complexity of MUC13 and its impact on cancer biology. Studies have shown that MUC13 overexpression can disrupt normal cellular polarity, leading to the acquisition of malignant traits. Furthermore, MUC13 has been associated with increased cancer plasticity, allowing cells to undergo epithelial-mesenchymal transition (E...
Source: Cancer and Metastasis Reviews - March 18, 2024 Category: Cancer & Oncology Source Type: research
Effects of second language acquisition on brain functional networks at different developmental stages
AbstractPrevious studies have shown that language acquisition influences both the structure and function of the brain. However, whether the acquisition of a second language at different periods of life alters functional network organization in different ways remains unclear. Here, functional magnetic resonance imaging data from 27 English-speaking monolingual controls and 52 Spanish –English bilingual individuals, including 22 early bilinguals who began learning a second language before the age of ten and 30 late bilinguals who started learning a second language at age fourteen or later, were collected from the OpenNeuro...
Source: Brain Imaging and Behavior - March 16, 2024 Category: Neurology Source Type: research
Cancers, Vol. 16, Pages 1171: Enhancing Leukemia Treatment: The Role of Combined Therapies Based on Amino Acid Starvation
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Cancer cells demand amino acids beyond their usage as “building blocks” for protein synthesis. As a result, targeting amino acid acquisition and utilization has emerged as a pivotal strategy in cancer treatment. In the setting of leukemia therapy, compelling examples of targeting amino acid metabolism exist at both pre-clinical and clinical stages. This review focuses on summarizing novel insights into the metabolism of glutamine, asparagine, arginine, and tryptophan in leukemias, and providing a comprehensive discussion of perturbing their metabolism to improve the therapeutic outcomes....
Source: Cancers - March 16, 2024 Category: Cancer & Oncology Authors: Can Chen Ji Zhang Tags: Review Source Type: research
Enhancing Opportunities for Decoding and Knowledge Building through Beginning Texts
AbstractEnsuring effective texts for student reading acquisition is a shared goal. This paper addresses the efficacy of decodable and leveled texts, their word features, and outcomes of reorganizing texts by vowel patterns and topics. Sparse evidence supports one text type's superiority in building a strong reading foundation. Further, the decoding demands of decodable and leveled texts diminish after initial instruction. Studies show reorganizing leveled texts according to a decoding curriculum aids reading acquisition. This finding is the basis for illustrating how existing texts can be reorganized to boost reading volum...
Source: Reading Teacher - March 15, 2024 Category: Child Development Authors: Elfrieda H. Hiebert Tags: Viewpoint Source Type: research
The Diagnostic Approach of Benign Esophageal Tumors: A Narrative Review
AbstractPurpose of ReviewBenign esophageal tumors (BETs) pose diagnostic challenges due to overlapping clinical and imaging features, necessitating advancements in diagnostic techniques. Distinguishing between tumors with and without malignant potential is essential to guide management. We aim to review the diagnostic approach to BETs with an emphasis on recent advances.Recent FindingsEUS-guided tissue acquisition is the current gold standard, particularly in subepithelial lesions (SELs). EUS with fine-needle biopsy (FNB) is considered superior to fine-needle aspiration (FNA), unless rapid on-site evaluation (ROSE) is avai...
Source: Current Treatment Options in Gastroenterology - March 15, 2024 Category: Gastroenterology Source Type: research
A case of severe Mycobacterium thermoresistibile pneumonia
We present a case of severe pneumonia associated with achalasia requiring intensive care unit treatment caused byMycobacterium thermoresistibile. We hypothesized the correlation between host and environmental factors leading to infection, citing its unique eponymous characteristics. We also reviewed published literature documenting cases of previous human infection with this organism. AbstractMycobacterium thermoresistibile is a thermotolerant nontuberculous mycobacterium which can rarely result in human infection. Although immunosuppression has been identified as a risk factor for infection, it is possible that mycobacter...
Source: Respirology Case Reports - March 14, 2024 Category: Respiratory Medicine Authors: Selvaraj Subramaniam,
Mrudula Kanhere,
Lisa Shephard,
Andrew Burke,
Sarah Saxon,
James Geake Tags: CASE REPORT Source Type: research
Strategies to enhance the level of service and safety of rural roads: A case study
by Qiannan Ai, Jun Zhang, Yuling Ye
Faced with the contradiction between the increasing traffic volume and the aging road infrastructures in the rural area, this paper aims to propose feasible strategies to enhance the level of service and safety, by a case study of the rural area in the north Jintan district. In order to figure out current issues related to rural roads, a carefully designed investigation has been conducted, and the results of the two-week investigation include roads’ basic information, traffic signs and protective facilities, surrounding landscape, and etc. Based on the field driving tests, specific pr...
Source: PLoS One - March 14, 2024 Category: Biomedical Science Authors: Qiannan Ai Source Type: research
Mathematical models of < i > Plasmodium vivax < /i > transmission: A scoping review
by Md Nurul Anwar, Lauren Smith, Angela Devine, Somya Mehra, Camelia R. Walker, Elizabeth Ivory, Eamon Conway, Ivo Mueller, James M. McCaw, Jennifer A. Flegg, Roslyn I. HicksonPlasmodium vivax is one of the most geographically widespread malaria parasites in the world, primarily found across South-East Asia, Latin America, and parts of Africa. One of the significant characteristics of theP. vivax parasite is its ability to remain dormant in the human liver as hypnozoites and subsequently reactivate after the initial infection (i.e. relapse infections). Mathematical modelling approaches have been widely applied to understan...
Source: PLoS Computational Biology - March 14, 2024 Category: Biology Authors: Md Nurul Anwar Source Type: research
The Impact of Human Immunodeficiency Virus on Women in the United States
Substantial improvements have been made in reducing HIV incidence rates among women in the United States. However, numerous disparities affect women ’s risk of HIV acquisition, in addition to affecting treatment outcomes for women living with HIV. As people with HIV continue to live longer due to antiretroviral therapy, clinicians must be cognizant of various health, financial, and social implications that can affect HIV self-management. Succe ssfully ending the HIV epidemic will require more targeted approaches on prevention, linkage to care, and treatment while also addressing underlying factors that affect women’s e...
Source: Nursing Clinics of North America - March 14, 2024 Category: Nursing Authors: Dominique Guillaume Source Type: research
Identification of gravity ‐responsive serum proteins in spaceflight mice using a quantitative proteomic approach with data‐independent acquisition mass spectrometry
This study examined serum proteome profiles in response to gravity unloading, and may help deepen our understanding of microgravity adaptation mechanisms during prolonged spaceflight missions. (Source: Proteomics)
Source: Proteomics - March 13, 2024 Category: Biochemistry Authors: Yayoi Kimura,
Yusuke Nakai,
Yoko Ino,
Tomoko Akiyama,
Kayano Moriyama,
Takashi Ohira,
Tomoyuki Saito,
Yutaka Inaba,
Ken Kumagai,
Akihide Ryo,
Hisashi Hirano Tags: RESEARCH ARTICLE Source Type: research
