Beyond 40 fluorescent probes for deep phenotyping of blood mononuclear cells, using spectral technology
The analytical capability of flow cytometry is crucial for differentiating the growing number of cell subsets found in human blood. This is important for accurate immunophenotyping of patients with few cells and a large number of parameters to monitor. Here, we present a 43-parameter panel to analyze peripheral blood mononuclear cells from healthy individuals using 41 fluorescence-labelled monoclonal antibodies, an autofluorescent channel, and a viability dye. We demonstrate minimal population distortions that lead to optimized population identification and reproducible results. We have applied an advanced approach in pane...
Source: Frontiers in Immunology - April 2, 2024 Category: Allergy & Immunology Source Type: research
Digital application for drug product potency target evaluation in biopharmaceutical manufacturing
AbstractBiopharmaceutical manufacturing entails a series of highly regulated steps. The manufacturing of safe and efficacious drug product (DP) requires testing of critical quality attributes (CQAs) against specification limits. DP potency concentration, which measures the dosage strength of a particular DP, is a CQA of great interest. In order to minimize the DP potency out-of-specification (OOS) risk, sterile fill finish (SFF) process adjustments may be needed. Varying the potency targets can be one such process adjustment. To facilitate such evaluation, data acquisition and statistical calculations are required. Regular...
Source: Biotechnology Progress - April 1, 2024 Category: Biotechnology Authors: Darrick Shen,
Shyam Panjwani,
Konstantinos Spetsieris Tags: RESEARCH ARTICLE Source Type: research
GSE262591 In-depth organic mass cytometry reveals differential contents of 3-hydroxybutanoic acid at the single-cell level
Contributors : Shaojie Qin ; Jiansen Lu ; Hongmin YuSeries Type : Expression profiling by high throughput sequencingOrganism : Homo sapiensIn-depth organic mass cytometry reveals differential contents of 3-hydroxybutanoic acid on single cell level. Single-cell transcriptome indicates the expression difference of BHB downstream anti-oxidative stress proteins such as MT2A while Fluorescence Activated Cell Sorting (FACS) assay validates positive relationship between BHB and target proteins, which suggests that contents heterogeneity of BHB may endow cancer cells with variant ability to resist surrounding oxidative stress. ...
Source: GEO: Gene Expression Omnibus - March 31, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Homo sapiens Source Type: research
GSE262587 TDG orchestrates ATF4-dependent gene transcription during retinoic acid-induced cell lineage committment
Contributors : Jeremie Poschmann ; Gilles Salbert ; Martin Braud ; Cynthia Fourgeux ; Thierry Madigou ; Marion TurpinSeries Type : Expression profiling by high throughput sequencingOrganism : Mus musculusCell differentiation during development is associated to large-scale modifications in the methylome that require the engagement of an active DNA demethylation machinery including Ten-Eleven-Translocation enzymes for oxidation of 5-methylcytosine and the T:G mismatch DNA glycosylase (TDG) for removal of the oxidized bases. Despite this well-defined molecular function, the biological output of TDG activity remains elus...
Source: GEO: Gene Expression Omnibus - March 31, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mus musculus Source Type: research
Effects of increased physical therapy staffing in the neonatal intensive care unit on oral feeding maturation and neurodevelopment of extremely low birth weight infants
It remains a matter of debate as to what extent early intervention may facilitate long-term functional outcomes of preterm infants in the neonatal intensive care unit (NICU). We aimed to examine the effect of increasing physical therapy (PT) staff dedicated to the NICU on temporal changes (initiation, duration) of PT interventions and functional outcomes (acquisition of full oral feeding and Hammersmith Neonatal Neurological Examination). (Source: Brain and Development)
Source: Brain and Development - March 30, 2024 Category: Neurology Authors: Yoshinori Morioka, Masayuki Nonogaki, Daiyu Kobayashi, Junji Nishimoto, Shigeru Obayashi Tags: Original Article Source Type: research
Computed Tomography Angiography After Transcatheter and Surgical Aortic Interventions
This article serves as a crucial resource for radiologists, offering invaluable insights into the complexities of aortic interventions and their subsequent imaging, fostering a comprehensive understanding of diagnostic and management strategies. (Source: Radiologic Clinics of North America)
Source: Radiologic Clinics of North America - March 28, 2024 Category: Radiology Authors: Ayaz Aghayev, Sumit Gupta, Michael Steigner Source Type: research
Sensors, Vol. 24, Pages 2185: Pattern Mining-Based Pig Behavior Analysis for Health and Welfare Monitoring
This study proposes a pattern mining-based pig behavior analysis system to provide visualized information and behavioral patterns, assisting farmers in effectively monitoring and assessing pigs’ health and welfare. The system consists of four modules: (1) data acquisition module for collecting pigs video; (2) detection and tracking module for localizing and uniquely identifying pigs, using tracking information to crop pig images; (3) pig behavior recognition module for recognizing pig behaviors from sequences of cropped images; and (4) pig behavior analysis module for providing visualized information and beha...
Source: Sensors - March 28, 2024 Category: Biotechnology Authors: Hassan Seif Mluba Othmane Atif Jonguk Lee Daihee Park Yongwha Chung Tags: Article Source Type: research
Correction: Proteomic signature associated with chronic kidney disease (CKD) progression identified by data-independent acquisition mass spectrometry
(Source: Clinical Proteomics)
Source: Clinical Proteomics - March 28, 2024 Category: Biochemistry Source Type: research
Cancers, Vol. 16, Pages 1311: Synergistic Effects of the RARalpha Agonist Tamibarotene and the Menin Inhibitor Revumenib in Acute Myeloid Leukemia Cells with KMT2A Rearrangement or NPM1 Mutation
In this study, the combination of revumenib and tamibarotene was investigated in various KMT2Ar or NPM1c AML cell lines and patient-derived blasts, focusing on the potential synergistic induction of differentiation or apoptosis. Both effects were analyzed by flow cytometry and validated by Western blot analysis. Synergy calculations were performed using viability assays. Regulation of the relevant key mediators for the MLL complex were quantified by RT-qPCR. In MV4:11 cells characterized by the highest relative mRNA levels of RARA, highly synergistic induction of apoptosis is demonstrated upon combination treatment. Induct...
Source: Cancers - March 28, 2024 Category: Cancer & Oncology Authors: Maximilian Fleischmann Julia Bechwar Diana Voigtl änder Mike Fischer Ulf Schnetzke Andreas Hochhaus Sebastian Scholl Tags: Article Source Type: research
Cancers, Vol. 16, Pages 1303: RNA m6a Methylation Regulator Expression in Castration-Resistant Prostate Cancer Progression and Its Genetic Associations
sna Batra
N6-methyladenosine (m6A) methylation, a prevalent epitranscriptomic modification, plays a crucial role in regulating mRNA expression, stability, and translation in mammals. M6A regulators have gained attention for their potential implications in tumorigenesis and clinical applications, such as cancer diagnosis and therapeutics. The existing literature predominantly addresses m6A regulators in the context of primary prostate cancer (PCa). However, a notable gap in the knowledge emerges regarding the dynamic expression patterns of these regulators as PCa progresses towards the castration-resistant stage (CRPC)....
Source: Cancers - March 27, 2024 Category: Cancer & Oncology Authors: Chamikara Liyanage Achala Fernando Audrey Chamberlain Afshin Moradi Jyotsna Batra Tags: Article Source Type: research
Soil phosphorus transformation and plant uptake driven by phosphate-solubilizing microorganisms
This article summarizes and analyzes how PSMs promote the absorption and utilization of P nutrients by plants from four perspectives: the types and functions of PSMs, phosphate-solubilizing mechanisms, main functional genes, and the impact of complex inoculation of PSMs on plant P acquisition. This article reviews the physiological and molecular mechanisms of phosphorus solubilization and growth promotion by PSMs, with a focus on analyzing the impact of PSMs on soil microbial communities and its interaction with root exudates. In order to better understand the ability of PSMs and their role in soil P transformation and to ...
Source: Frontiers in Microbiology - March 27, 2024 Category: Microbiology Source Type: research
HV & I Reviewer Highlight
Volume 20, Issue 1, December 2024 . (Source: Human Vaccines and Immunotherapeutics)
Source: Human Vaccines and Immunotherapeutics - March 27, 2024 Category: Allergy & Immunology Authors: Ronald EllisAdam Weissa Editor-in-Chief, Jerusalem, Israelb Acquisitions Editor, Taylor & Francis Group Source Type: research
Denoised single-pixel imaging in a Fourier acquisition mode
The degradation of imaging quality caused by environmental noise during Fourier single pixel imaging (FSPI) is a big problem. In this ... (Source: Applied Optics)
Source: Applied Optics - March 26, 2024 Category: Physics Authors: Pengcheng Xia Le Wang Shengmei Zhao Source Type: research
An Automated Tool to Classify and Transform Unstructured MRI Data into BIDS Datasets
AbstractThe increasing use of neuroimaging in clinical research has driven the creation of many large imaging datasets. However, these datasets often rely on inconsistent naming conventions in image file headers to describe acquisition, and time-consuming manual curation is necessary. Therefore, we sought to automate the process of classifying and organizing magnetic resonance imaging (MRI) data according to acquisition types common to the clinical routine, as well as automate the transformation of raw, unstructured images into Brain Imaging Data Structure (BIDS) datasets. To do this, we trained an XGBoost model to classif...
Source: Neuroinformatics - March 26, 2024 Category: Neuroscience Source Type: research
Pneumococcal conjugate vaccination schedules in infants —acquisition, immunogenicity, and pneumococcal conjugate and yellow fever vaccine co-administration study: statistical analysis plan
The effectiveness of immunisation with pneumococcal conjugate vaccine (PCV) has been demonstrated in many countries. However, the global impact of PCV is limited by its cost, which has prevented its introducti... (Source: Trials)
Source: Trials - March 26, 2024 Category: General Medicine Authors: Grant A. Mackenzie, Isaac Osei, Rasheed Salaudeen, Paul V. Licciardi, Brian Greenwood, Kim Mulholland and Cattram Nguyen Tags: Update Source Type: research
