Molecules, Vol. 29, Pages 587: Identification and Validation of Magnolol Biosynthesis Genes in Magnolia officinalis
In this study, we have proposed a one-step conversion of magnolol from chavicol using laccase. After leveraging 20 transcriptomes from diverse parts of M. officinalis, transcripts were assembled, enriching genome annotation. Upon integrating this dataset with current genomic information, we could identify 30 laccase enzymes. From two potential gene clusters associated with magnolol production, highly expressed genes were subjected to functional analysis. In vitro experiments confirmed MoLAC14 as a pivotal enzyme in magnolol synthesis. Improvements in the thermal stability of MoLAC14 were achieved through selective mutation...
Source: Molecules - January 25, 2024 Category: Chemistry Authors: Yue Yang Zihe Li Hang Zong Shimeng Liu Qiuhui Du Hao Wu Zhenzhu Li Xiao Wang Lihui Huang Changlong Lai Meide Zhang Wen Wang Xianqing Chen Tags: Article Source Type: research
Inhibition of Ca < sup > 2+ < /sup > -permeable TRPV3 and inflammatory cytokine release by honokiol and magnolol in human epidermal keratinocytes
Biochem Biophys Res Commun. 2023 Nov 27;692:149332. doi: 10.1016/j.bbrc.2023.149332. Online ahead of print.ABSTRACTTransient receptor potential vanilloid-3 (TRPV3) ion channels are prominently expressed in keratinocytes, playing a vital role in skin functions. Honokiol and magnolol (H&M) the primary bioactive constituents in Magnolia officinalis extract, demonstrate anti-inflammatory and skin-protective properties. Nevertheless, the underlying mechanism regarding their effect on Ca2+-permeable ion channels remain unclear. Our purpose in this study is to investigate the effect of H&M on TRPV3 and cytokine release in...
Source: Biochemical and Biophysical Research communications - December 3, 2023 Category: Biochemistry Authors: Huyen Dang Thi Ji Yeong Kim Hyun Jong Kim Woo Kyung Kim Sung Joon Kim Joo Hyun Nam Source Type: research
