On the Mechanisms of Hypohalous Acid Formation and Electrophilic Halogenation by Non-Native Halogenases
Angew Chem Int Ed Engl. 2024 Apr 12:e202403858. doi: 10.1002/anie.202403858. Online ahead of print.ABSTRACTEnzymatic electrophilic halogenation is a mild tool for functionalization of diverse organic compounds. Only a few groups of native halogenases are capable of catalyzing such a reaction. Here, we used a mechanism-guided strategy to discover the electrophilic halogenation activity catalyzed by non-native halogenases. As the ability to form a hypohalous acid (HOX) is key for halogenation, flavin-dependent monooxygenases/oxidases capable of forming C4a-hydroperoxyflavin (FlC4a-OOH) such as dehalogenase, hydroxylases, luc...
Source: Angewandte Chemie - April 12, 2024 Category: Chemistry Authors: Kridsadakorn Prakinee Narin Lawan Aisaraphon Phintha Surawit Visitsatthawong Penchit Chitnumsub Watcharapa Jitkaroon Pimchai Chaiyen Source Type: research
Transcriptomic profiling analysis of the effect of palmitic acid on 3D spheroids of β-like cells derived from induced pluripotent stem cells
Gene. 2024 Apr 10:148441. doi: 10.1016/j.gene.2024.148441. Online ahead of print.ABSTRACTType 2 diabetes (T2D) is posing a serious public health concern with a considerable impact on human life and health expenditures worldwide. The disease develops when insulin plasma level is insufficient for coping insulin resistance, caused by the decline of pancreatic β-cell function and mass. In β-cells, the lipotoxicity exerted by saturated free fatty acids in particular palmitate (PA), which is chronically elevated in T2D, plays a major role in β-cell dysfunction and mass. However, there is a lack of human relevant in vitro mode...
Source: Gene - April 12, 2024 Category: Genetics & Stem Cells Authors: Lisa Morisseau Fumiya Tokito Mathilde Lucas St éphane Poulain Soo Hyeon Kim Val érie Plaisance Val érie Pawlowski C écile Legallais Rachid Jellali Yasuyuki Sakai Amar Abderrahmani Eric Leclerc Source Type: research
Explaining ethical challenges and practical solutions from a nursing managements' perspective: A phenomenological study
CONCLUSION: Nurse managers are always faced with ethical challenges in their daily activities. To deal with these challenges, they must be familiar with the rules and principles of professional ethics, practical standards, and laws related to patients' rights. Ethical principles and awareness of the ethical decision-making process are useful and effective in having capable and ethical nurses who provide competent care. To deal with ethical challenges, it is suggested to consider self-care strategies and structural and specialized support in each center, which will ultimately lead to the improvement of service quality and t...
Source: Primary Care - April 12, 2024 Category: Primary Care Authors: Mahboobeh Khosravani Majid Taheri Nazanin Amini Nasrin Babaeian Saeed Lak Nazi Nejat Source Type: research
Peptide Extract from Red Kidney Beans, < em > Phaseolus vulgaris < /em > (Fabaceae), Shows Promising Antimicrobial, Antibiofilm, and Quorum Sensing Inhibitory Effects
In this study, peptides were extracted from the seeds of Phaseolus vulgaris (Fabaceae), and the antimicrobial activities of the extract were evaluated using microbroth dilution against five different microorganisms. The extract showed antimicrobial activity against all tested organisms with minimum inhibitory concentrations (MIC) of 2.5 mg/mL, except for Candida albicans and Pseudomonas aeruginosa, which had MICs of 1.25 mg/mL. The extract was also bacteriostatic for all tested organisms. The crude peptide extract from Phaseolus vulgaris was further studied for its antibiofilm activity against Pseudomonas aeruginosa, a com...
Source: Cell Research - April 12, 2024 Category: Cytology Authors: Jennifer Tetteh De-Youngster Wereko Brobbey Kofi Junior Osei Azumah Ayamah Michael Konney Laryea Godfred Darko Lawrence Sheringham Borquaye Source Type: research
Enhanced bactericidal, antibiofilm and antioxidative response of Lawsonia inermis leaf extract synthesized ZnO NPs loaded with commercial antibiotic
Bioprocess Biosyst Eng. 2024 Apr 12. doi: 10.1007/s00449-024-03000-9. Online ahead of print.ABSTRACTGlobally, antibiotic resistance is a challenging issue in healthcare sector. The emergence of multiple drug-resistant bacteria has forced us to modify existing medicines and or formulate newer medicines that are effective and inexpensive. In this perspective, this study involves the formation of zinc oxide nanoparticles (ZnO NPs) by utilizing the Lawsonia inermis (Li) leaf extract. The prepared L. inermis leaf extract mediated ZnO NPs (Li-ZnO NPs) were bio-physically characterized. The antibacterial and radical scavenging ef...
Source: Bioprocess and Biosystems Engineering - April 12, 2024 Category: Biomedical Engineering Authors: Balasubramanian Malaikozhundan Sonaimuthu Mohandoss Raman Krishnamoorthi Palanichamy Vidhya Bharathi Subramanian Palanisamy Jayaraj Vinodhini Source Type: research
The social and healthcare professional support drawn upon by women antenatally during the COVID-19 pandemic: A recurrent, cross-sectional, thematic analysis
CONCLUSIONS: At T1, anxieties were ascribed to the exclusion of partners from routine care, and to perceived insensitivity and aggression from the public. For T2, insufficient Governmental transparency led to disillusionment, confusion, and anger. Covert workplace discrimination also caused distress at T2. Across timepoints: deteriorated mental wellbeing was attributed to depleted opportunities to interact socially and scaled back maternity care.IMPLICATIONS FOR PRACTICE: Recommendations are made to: protect maternal autonomy; improve quality of mental health and routine care signposting; prioritise parental community supp...
Source: Midwifery - April 12, 2024 Category: Midwifery Authors: Leanne Jackson Si ân M Davies Monic Gaspar Anastasija Podkujko Joanne A Harrold Leonardo DE Pascalis Victoria Fallon Laura K Soulsby Sergio A Silverio Source Type: research
Understanding and Quantifying Network Robustness to Stochastic Inputs
Bull Math Biol. 2024 Apr 12;86(5):55. doi: 10.1007/s11538-024-01283-3.ABSTRACTA variety of biomedical systems are modeled by networks of deterministic differential equations with stochastic inputs. In some cases, the network output is remarkably constant despite a randomly fluctuating input. In the context of biochemistry and cell biology, chemical reaction networks and multistage processes with this property are called robust. Similarly, the notion of a forgiving drug in pharmacology is a medication that maintains therapeutic effect despite lapses in patient adherence to the prescribed regimen. What makes a network robust...
Source: Bulletin of Mathematical Biology - April 12, 2024 Category: Bioinformatics Authors: Hwai-Ray Tung Sean D Lawley Source Type: research
On the Mechanisms of Hypohalous Acid Formation and Electrophilic Halogenation by Non-Native Halogenases
Angew Chem Int Ed Engl. 2024 Apr 12:e202403858. doi: 10.1002/anie.202403858. Online ahead of print.ABSTRACTEnzymatic electrophilic halogenation is a mild tool for functionalization of diverse organic compounds. Only a few groups of native halogenases are capable of catalyzing such a reaction. Here, we used a mechanism-guided strategy to discover the electrophilic halogenation activity catalyzed by non-native halogenases. As the ability to form a hypohalous acid (HOX) is key for halogenation, flavin-dependent monooxygenases/oxidases capable of forming C4a-hydroperoxyflavin (FlC4a-OOH) such as dehalogenase, hydroxylases, luc...
Source: Angewandte Chemie - April 12, 2024 Category: Chemistry Authors: Kridsadakorn Prakinee Narin Lawan Aisaraphon Phintha Surawit Visitsatthawong Penchit Chitnumsub Watcharapa Jitkaroon Pimchai Chaiyen Source Type: research
Explaining ethical challenges and practical solutions from a nursing managements' perspective: A phenomenological study
CONCLUSION: Nurse managers are always faced with ethical challenges in their daily activities. To deal with these challenges, they must be familiar with the rules and principles of professional ethics, practical standards, and laws related to patients' rights. Ethical principles and awareness of the ethical decision-making process are useful and effective in having capable and ethical nurses who provide competent care. To deal with ethical challenges, it is suggested to consider self-care strategies and structural and specialized support in each center, which will ultimately lead to the improvement of service quality and t...
Source: Primary Care - April 12, 2024 Category: Primary Care Authors: Mahboobeh Khosravani Majid Taheri Nazanin Amini Nasrin Babaeian Saeed Lak Nazi Nejat Source Type: research
Patient experiences using public and private insurance coverage for abortion in Illinois: Implementation successes and remaining gaps
ConclusionWhen abortion was fully covered by insurance, it reduced financial burdens and enhanced reproductive autonomy. Illinois Medicaid policy —with seamless enrollment options and appropriate reimbursement rates—offers a model for improving abortion access in other states. Further investigation is needed to determine compliance among private insurance companies and increase transparency. (Source: Perspectives on Sexual and Reproductive Health)
Source: Perspectives on Sexual and Reproductive Health - April 12, 2024 Category: Sexual Medicine Authors: Madeline Quasebarth,
Madeleine Boesche,
Tecora Turner,
Amy Moore,
Danielle Young,
Debra Stulberg,
Lee Hasselbacher Tags: SPECIAL ISSUE ARTICLE Source Type: research
Assessing causal links between age at menarche and adolescent mental health: a Mendelian randomisation study
The timing of puberty may have an important impact on adolescent mental health. In particular, earlier age at menarche has been associated with elevated rates of depression in adolescents. Previous research su... (Source: BMC Medicine)
Source: BMC Medicine - April 12, 2024 Category: Internal Medicine Authors: Adrian Dahl Askelund, Robyn E. Wootton, Fartein A. Torvik, Rebecca B. Lawn, Helga Ask, Elizabeth C. Corfield, Maria C. Magnus, Ted Reichborn-Kjennerud, Per M. Magnus, Ole A. Andreassen, Camilla Stoltenberg, George Davey Smith, Neil M. Davies, Alexandra Ha Tags: REGISTERED REPORT Source Type: research
“Walking on Eggshells”: Will PIEZO-ICSI emerge as a new standard for penetrating the PIEZona pellucida?
(Source: Fertility and Sterility)
Source: Fertility and Sterility - April 12, 2024 Category: Reproduction Medicine Authors: Stephanie Hallisey, Alison Bartolucci, Lawrence Engmann Tags: Reflections Source Type: research
Molecules, Vol. 29, Pages 1763: Hydrocarbon Transportation in Heterogeneous Shale Pores by Molecular Dynamic Simulation
This study is of great significance for understanding the occurrence characteristics and flow mechanism of shale oil within inorganic nanopores. (Source: Molecules)
Source: Molecules - April 12, 2024 Category: Chemistry Authors: Shuo Sun Mingyu Gao Shuang Liang Yikun Liu Tags: Article Source Type: research
Examining confidential wholesale margin estimates in European countries for the price negotiation of patented drugs in Germany: a statistical model
Based on the legal framework laid down in section 130b (9) of Book V of the German Social Code, various criteria are relevant for the negotiated price for new patented drugs in Germany. European reference pric... (Source: Health Economics Review)
Source: Health Economics Review - April 12, 2024 Category: Health Management Authors: Iris an der Heiden, Guido Schiffhorst, Laura M üller, Martin Albrecht, Arne Bartol and Stefanie Wiberny Tags: Research Source Type: research
Examining confidential wholesale margin estimates in European countries for the price negotiation of patented drugs in Germany: a statistical model
ConclusionIf there is reason to believe that margins are regressive, confidential wholesale margins are expected to be better estimated by the power function based on margins of regulated countries than by the published country-specific average margins, reducing significantly inaccurate effects on margin estimations of high-price drugs. (Source: Health Economics Review)
Source: Health Economics Review - April 12, 2024 Category: International Medicine & Public Health Source Type: research
