Bacterial proteome adaptation during fermentation in dairy environments
Food Microbiol. 2024 Aug;121:104514. doi: 10.1016/j.fm.2024.104514. Epub 2024 Mar 2.ABSTRACTThe enzymatic repertoire of starter cultures belonging to the Lactococcus genus determines various important characteristics of fermented dairy products but might change in response to the substantial environmental changes in the manufacturing process. Assessing bacterial proteome adaptation in dairy and other food environments is challenging due to the high matrix-protein concentration and is even further complicated in particularly cheese by the high fat concentrations, the semi-solid state of that matrix, and the non-growing stat...
Source: Food Microbiology - April 18, 2024 Category: Food Science Authors: Berdien van Olst Avis Nugroho Sjef Boeren Jacques Vervoort Herwig Bachmann Michiel Kleerebezem Source Type: research
Primer Part 1 - Preparing a laboratory quality improvement project
Clin Biochem. 2024 Apr 16:110764. doi: 10.1016/j.clinbiochem.2024.110764. Online ahead of print.ABSTRACTQuality in laboratory medicine encompasses multiple components related to total quality management, including quality control (QC), quality assurance (QA), quality indicators, and quality improvement (QI). Together, they contribute to minimizing errors (pre-analytical, analytical, or post-analytical) in clinical service delivery and improving process appropriateness and efficiency. In contrast to static quality benchmarks (QC, QA, quality indicators), the QI paradigm is a continuous approach to systemic process improveme...
Source: Clinical Biochemistry - April 18, 2024 Category: Biochemistry Authors: Mary Kathryn Bohn Roy Augustin Lucas Chartier Luke Devine Samik Doshi Leanne Ginty Elliot Lass Felix Leung William Mundle Graeme Nimmo Alyson Sandy Kelly Shillington Amanda Simon Amanda Steiman Ahmed Taher Cindy Tang Friesner Cristina Zanchetta Jennifer T Source Type: research
Repeated Divergence in Opsin Gene Expression Mirrors Photic Habitat Changes in Rapidly Evolving Crater Lake Cichlid Fishes
Am Nat. 2024 May;203(5):604-617. doi: 10.1086/729420. Epub 2024 Mar 15.ABSTRACTAbstractSelection pressures differ along environmental gradients, and traits tightly linked to fitness (e.g., the visual system) are expected to track such variation. Along gradients, adaptation to local conditions might be due to heritable and nonheritable environmentally induced variation. Disentangling these sources of phenotypic variation requires studying closely related populations in nature and in the laboratory. The Nicaraguan lakes represent an environmental gradient in photic conditions from clear crater lakes to very turbid great lake...
Source: The American Naturalist - April 18, 2024 Category: Biology Authors: C ésar Bertinetti Andreas H ärer Nidal Karagic Axel Meyer Juli án Torres-Dowdall Source Type: research
Circadian Disruption as a Risk Factor for Development of Cardiovascular and Metabolic Disorders - From Animal Models to Human Population
Physiol Res. 2024 Apr 18. Online ahead of print.ABSTRACTThe lifestyle of human society is drifting apart from the natural environmental cycles that have influenced it since its inception. These cycles were fundamental in structuring the daily lives of people in the pre-industrial era, whether they were seasonal or daily. Factors that disrupt the regularity of human behaviour and its alignment with solar cycles, such as late night activities accompanied with food intake, greatly disturb the internal temporal organization in the body. This is believed to contribute to the rise of the so-called diseases of civilization. In th...
Source: Physiological Research - April 18, 2024 Category: Physiology Authors: A Sumov á M Sl ádek Source Type: research
Improved synthesis and application of an alkyne-functionalized isoprenoid analogue to study the prenylomes of motor neurons, astrocytes and their stem cell progenitors
Bioorg Chem. 2024 Apr 16;147:107365. doi: 10.1016/j.bioorg.2024.107365. Online ahead of print.ABSTRACTProtein prenylation is one example of a broad class of post-translational modifications where proteins are covalently linked to various hydrophobic moieties. To globally identify and monitor levels of all prenylated proteins in a cell simultaneously, our laboratory and others have developed chemical proteomic approaches that rely on the metabolic incorporation of isoprenoid analogues bearing bio-orthogonal functionality followed by enrichment and subsequent quantitative proteomic analysis. Here, several improvements in the...
Source: Bioorganic Chemistry - April 18, 2024 Category: Chemistry Authors: Kiall F Suazo Vartika Mishra Sanjay Maity Shelby A Auger Katarzyna Justyna Alexandru M Petre Linda Ottoboni Jessica Ongaro Stefania P Corti Francesco Lotti Serge Przedborski Mark D Distefano Source Type: research
Dengue dynamics: Prognostic and disease monitoring through molecular and serological profiling of clinical isolates
CONCLUSION: Our laboratory effectively standardized an RT-PCR-based approach for molecular identification of dengue virus in clinical specimens. This adaptive technique which used numerous primer sets displayed good specificity and sensitivity in serotype detection. The technology provides for quick and reliable identification of dengue virus infections, allowing for targeted treatment and preventative actions for successful disease management in highly populated regions.PMID:38634367 | DOI:10.4103/JVBD.JVBD_202_23 (Source: Journal of Vector Borne Diseases)
Source: Journal of Vector Borne Diseases - April 18, 2024 Category: Infectious Diseases Authors: Vikas Tiwari Mohd Afzal Abhishek Sharma Jaishree Tiwari Source Type: research
Enhance the physicochemical properties of the citronella oil -SLNPs to improve the insecticidal activity against < em > Spodoptera littoralis < /em > (Boisd) (Lep. Nactuidae)
Nat Prod Res. 2024 Apr 18:1-17. doi: 10.1080/14786419.2024.2342563. Online ahead of print.ABSTRACTThe Nano-formulation of citronella essential oil (Cymbopogon nardus (L.) and their mixtures of three adjuvants (Provecta®, Top film®, and PEG600-dioleate) were studied to enhance physico-chemical properties of the Nano-formulation and improve the insecticidal effect against Spodoptera littoralis (Boisd). Characterisation of physicochemical properties of Nano-formulation was studied by calculating droplet size, polydispersity index (PDI), and Zeta-potential parameters. The results showed that the Solid Lipid Nanoparticles (SL...
Source: Natural Product Research - April 18, 2024 Category: Biochemistry Authors: Manal Mohamed Adel Nagwa Yoseif Salem Hany Mohamed Hussein Source Type: research
Development and performance of NLISA for C-reactive protein detection based on Prussian blue nanoparticle conjugates
Anal Bioanal Chem. 2024 Apr 18. doi: 10.1007/s00216-024-05268-y. Online ahead of print.ABSTRACTPrussian blue nanoparticles (PBNPs), also called nanozymes, are very attractive as an alternative to horseradish peroxidase in immunoassay development due to their simple and low-cost synthesis, stability and high catalytic activity. Today, there is a method for highly effective PBNP synthesis based on the reduction of an FeCl3/K3[Fe(CN)6] mixture by hydrogen peroxide. However, there is a lack of research showcasing the use of these highly effective PBNPs for specific target detection in clinical settings, as well as a lack of co...
Source: Analytical and Bioanalytical Chemistry - April 18, 2024 Category: Chemistry Authors: Maria Nikitina Pavel Khramtsov Maria Bochkova Mikhail Rayev Source Type: research
24-h passive heat and cold exposures did not modify energy intake and appetite but strongly modify food reward
Br J Nutr. 2024 Apr 18:1-40. doi: 10.1017/S0007114524000825. Online ahead of print.ABSTRACTEffects of acute thermal exposures on appetite appear hypothetical in reason of very heterogeneous methodologies. The aim of this study was therefore to clearly define the effects of passive 24-h cold (16°C) and heat (32°C) exposures on appetitive responses compared to a thermo neutral condition (24°C). Twenty-three healthy, young, and active male participants realised three sessions (from 1 pm) in a laboratory conceived like an apartment dressed with the same outfit (Clo=1). Three meals composed of three or four cold or warm dish...
Source: The British Journal of Nutrition - April 18, 2024 Category: Nutrition Authors: Maxime Coca Louis Besan çon M égane Erblang St éphanie Bourdon Arnaud Gruel Beno ît Lepetit Vincent Beauchamps Blandine Tavard Pauline Oustric Graham Finlayson David Thivel Alexandra Malgoyre Pierre-Emmanuel Tardo-Dino Cyprien Bourrilhon Keyne Charlot Source Type: research
Wide use of broad-spectrum antibiotics in very low birth weight infants with spontaneous focal intestinal perforation —is it really justified?
ConclusionThe use of carbapenems as broad-spectrum antimicrobials agents might not be justified in most VLBW infants with FIP. Knowledge on the development of the neonatal gut microbiota, local resistance patterns and individual microbiological findings should be taken into consideration when implementing antimicrobial stewardship programs (ASPs). (Source: Infection)
Source: Infection - April 18, 2024 Category: Infectious Diseases Source Type: research
Non-betahemolytic streptococcal bacteremia, cardiac implantable electronic device, endocarditis, extraction, and outcome; a population-based retrospective cohort study
ConclusionThe study did not find a high risk of IE in patients with NBHSB and CIED. Most cases of IE were in conjunction with a simultaneous HVP. A management algorithm is suggested. (Source: Infection)
Source: Infection - April 18, 2024 Category: Infectious Diseases Source Type: research
The predictive power of data: machine learning analysis for Covid-19 mortality based on personal, clinical, preclinical, and laboratory variables in a case –control study
The COVID-19 pandemic has presented unprecedented public health challenges worldwide. Understanding the factors contributing to COVID-19 mortality is critical for effective management and intervention strategi... (Source: BMC Infectious Diseases)
Source: BMC Infectious Diseases - April 18, 2024 Category: Infectious Diseases Authors: Maryam Seyedtabib, Roya Najafi-Vosough and Naser Kamyari Tags: Research Source Type: research
Implementation of a Three-Way Comparability Assessment for a Bioanalytical Anti-Drug Antibody Method
AbstractImmunogenicity evaluation is a critical part of drug development. Regulatory guidelines from multiple health agencies provide recommendations for the development and validation of anti-drug antibody (ADA) assays to assess immunogenicity in clinical trials. These recommendations primarily describe an ADA method run in one bioanalytical laboratory supporting a biotherapeutic molecule; however, there are increasing instances that may necessitate the support of the ADA method being run in more than one laboratory. A program can rapidly expand into multiple clinical studies within one or multiple countries, where the mo...
Source: The AAPS Journal - April 18, 2024 Category: Drugs & Pharmacology Source Type: research
Seasonal discrepancy of airborne fungal diversity and community structure in Lentinula edodes factory
. (Source: Mycology)
Source: Mycology - April 18, 2024 Category: Biology Authors: Wenbing GongKaixiao QiuZhen ZhuangLujun ZhangXueqing LiuXiao YuYinbing BianYang Xiaoa State Key Laboratory of Agricultural Microbiology, Hubei Hongshan Laboratory, Institute of Applied Mycology, Huazhong Agricultural University, Wuhan, Chinab Institute of Source Type: research
Emergence of OXA-48-producing Klebsiella pneumoniae in Lithuania, 2023: a multi-cluster, multi-hospital outbreak
In 2023, an increase of OXA-48-producingKlebsiella pneumoniae was noticed by the Lithuanian National Public Health Surveillance Laboratory. Whole genome sequencing (WGS) of 106 OXA-48-producingK. pneumoniae isolates revealed three distinct clusters of carbapenemase-producingK. pneumoniae high-risk clones, including sequence type (ST) 45 (n = 35 isolates), ST392 (n = 32) and ST395 (n = 28), involving six, six and nine hospitals in different regions, respectively. These results enabled targeted investigation and control, and underscore the value of national WGS-based surveillance for antim...
Source: Eurosurveillance - April 18, 2024 Category: Infectious Diseases Authors: Paulius Grei čius, Marius Linkevicius, Jelena Razmuk, Jekaterina Sinotova, Erik Alm, Olov Svartström, Valeria Bortolaia, Eglė Kudirkienė, Louise Roer, Rene S Hendriksen, Gabija Tamoliūnaitė, Daniel Palm, Dominique L Monnet, Anke Kohlenberg and Algir Source Type: research
