Growth of Neonatal Hemodynamics Programs and Targeted Neonatal Echocardiography Performed by Neonatologists
The field of Neonatal Hemodynamics was established to elevate the standard of care for premature infants with hemodynamic instability, based on the academic principals of subspecialization, research, and clinical collaboration. Successful implementation of Hemodynamics programs across North America has resulted from strong collaboration between neonatologists with formal training in Targeted Neonatal Echocardiography (TnECHO) and Pediatric Echocardiography Laboratory leaders. The development of standardized imaging protocols and international collaborative groups has led to the generation of high-quality data for research ...
Source: Journal of the American Society of Echocardiography - September 30, 2020 Category: Cardiology Authors: Patrick McNamara, Wyman Lai Tags: American Society of Echocardiography News Source Type: research

Continuing Education and Meeting Calendar
The American Society of Echocardiography is accredited by the Accreditation Council for Continuing Medical Education to provide continuing medical education for physicians. ASE recognizes courses as supplements to formal training in an established echocardiographic laboratory. For more information about a course, please call the number listed. To list a course in the Continuing Education and Meeting Calendar, send the date(s), title, location, provider, course director(s), and contact information to ASE, Attn: Education Dept, 2530 Meridian Parkway, Durham, NC 27713; Tel: 919-861-5574 x7161; E-mail: Education@ASEcho.org. (S...
Source: Journal of the American Society of Echocardiography - September 30, 2020 Category: Cardiology Tags: American Society of Echocardiography News Source Type: research

Harmonization of Molecular Testing for Non-Small Cell Lung Cancer: Emphasis on PD-L1
Comprehensive molecular testing plays a critical role in the choice of treatment for non-small lung cell cancer (NSCLC). The analysis of druggable alterations in EGFR, BRAF, MET, KRAS, ALK, ROS1, RET and NTRK1/2/3 genes is more or less standardized and can be achieved using a single diagnostic platform, e.g., next generation sequencing (NGS) or polymerase chain reaction (PCR). In contrast to above targets, PD-L1 testing requires the use of immunohistochemistry (IHC). There are multiple PD-L1 IHC assays, which utilize distinct antibodies and detection systems. These PD-L1 tests are tailored to distinct drugs, often rely on ...
Source: Frontiers in Oncology - September 30, 2020 Category: Cancer & Oncology Source Type: research

Early Mortality in Children and Adolescents with Acute Promyelocytic Leukemia: Experience of the Boldrini Children’s Center
Conclusions: EDs were mainly caused by thrombohemorrhagic events and occurred within the second week after diagnosis. High peripheral promyelocytes and creatinine levels were predictors of ED in APL. (Source: Journal of Pediatric Hematology Oncology)
Source: Journal of Pediatric Hematology Oncology - September 29, 2020 Category: Hematology Tags: Online Articles: Clinical and Laboratory Observations Source Type: research

Obesity as a Potential Risk Factor for Vincristine-induced Peripheral Neuropathy
Acute lymphoblastic leukemia (ALL) is the most common pediatric cancer. Vincristine is a core chemotherapeutic agent for patients with ALL; unfortunately, ∼78% will develop vincristine-induced peripheral neuropathy (VIPN). VIPN can result in vincristine dose reductions that decrease therapeutic efficacy: making it important to understand which children are at highest risk for VIPN. We hypothesized that pediatric ALL patients who were obese at diagnosis would develop worse VIPN than healthy weight children with ALL within the first year. Our results confirmed that obese pediatric patients have significantly (P=0.03) wor...
Source: Journal of Pediatric Hematology Oncology - September 29, 2020 Category: Hematology Tags: Online Articles: Clinical and Laboratory Observations Source Type: research

Mutation of CEP72 Gene May Predispose Patients to Hepatotoxicity
This report describes hepatotoxicity that first developed during consolidation in a patient homozygous for the CEP72 risk alleles. Bilirubin levels normalized following dose reduction of 6-mercaptopurine. The patient continues to tolerate maintenance therapy at a reduced dose of 6-mercaptopurine. (Source: Journal of Pediatric Hematology Oncology)
Source: Journal of Pediatric Hematology Oncology - September 29, 2020 Category: Hematology Tags: Online Articles: Clinical and Laboratory Observations Source Type: research

Growing Teratoma Syndrome After Chemotherapy For Ovarian Immature Teratoma
We report the development and management of this phenomenon in a pediatric patient who had several recurrences of her IT. (Source: Journal of Pediatric Hematology Oncology)
Source: Journal of Pediatric Hematology Oncology - September 29, 2020 Category: Hematology Tags: Online Articles: Clinical and Laboratory Observations Source Type: research

Different Clinical Presentation of 3 Children With Familial Hemophagocytic Lymphohistiocytosis With 2 Novel Mutations
Although familial hemophagocytic lymphohistiocytosis (FHL) generally manifest with a combination of unremitting fever, hepatosplenomegaly, and pancytopenia; unusual presentations should also be taken into account. Herein, we present 3 FHL cases with 2 novel mutations with different initial presentations. The first patient bearing a homozygous truncation mutation in UNC13D (c.2650C>T.p.Gln884Ter) presented with central nervous system involvement and skin rash. The patient responded to the HLH-2004 protocol, and allogenic hematopoietic stem cell transplantation was performed from her healthy sister. The second and third p...
Source: Journal of Pediatric Hematology Oncology - September 29, 2020 Category: Hematology Tags: Online Articles: Clinical and Laboratory Observations Source Type: research

Pediatric CNS Tumor Patients Have Significantly Lower Requirement of Tissue Plasminogen Activator for Episodes of Central Venous Catheter Dysfunction Compared With Other Pediatric Oncology Patients: Results of a Population-based Cohort Study
Conclusions: Our study showed that pediatric patients with CNS tumors require significantly less frequent administration of tPA for episodes of CVC dysfunction than patients with non-CNS tumors after adjusting for confounding factors. Hypotheses for this include: potential biologic differences of tumors, the role of the blood-brain barrier, or systematic differences in intensity of treatments. (Source: Journal of Pediatric Hematology Oncology)
Source: Journal of Pediatric Hematology Oncology - September 29, 2020 Category: Hematology Tags: Online Articles: Clinical and Laboratory Observations Source Type: research

Langerhans’ Cell Histiocytosis Masquerading as Caroli’s Disease
We describe here the clinical details of a 2-year-old girl with involvement of unusual sites like the parotid glands and the nails. This child also had multiple cystic lesions in the liver leading to a misdiagnosis of Caroli’s disease. Knowledge about the uncommon manifestations of this rare disorder helps in early diagnosis and treatment. (Source: Journal of Pediatric Hematology Oncology)
Source: Journal of Pediatric Hematology Oncology - September 29, 2020 Category: Hematology Tags: Online Articles: Clinical and Laboratory Observations Source Type: research

Germline MUTYH Mutation in a Pediatric Cancer Survivor Developing a Secondary Malignancy
Radiotherapy-induced second malignant neoplasms (SMNs) are a severe late complication in pediatric cancer survivors. Germline mutations in tumor suppressor genes contribute to SMNs; however, the most relevant germline variants mediating susceptibility are not fully defined. The authors performed matched whole-exome sequencing analyses of germline and tumor DNA from 4 pediatric solid tumor survivors who subsequently developed radiation-associated SMNs. Pathogenic and predicted deleterious germline variants were identified for each patient and validated with Sanger sequencing. These germline variants were compared with germl...
Source: Journal of Pediatric Hematology Oncology - September 29, 2020 Category: Hematology Tags: Online Articles: Clinical and Laboratory Observations Source Type: research

Rosette-forming Glioneuronal Tumor in the Optic Pathway of a Child
We describe an unusual presentation of rosette-forming glioneuronal tumor occurring in the optic pathway in a child. As the site of the tumor was not amenable to resection, he underwent radiotherapy and is currently well on follow-up. (Source: Journal of Pediatric Hematology Oncology)
Source: Journal of Pediatric Hematology Oncology - September 29, 2020 Category: Hematology Tags: Online Articles: Clinical and Laboratory Observations Source Type: research

Sustained Remission After Maintenance Irinotecan in Patient With Multiply Relapsed Hepatoblastoma
Children with recurrent hepatoblastoma have a poor prognosis and limited treatment options. The authors describe a child with metastatic hepatoblastoma who had multiple relapses. He was treated with surgical resection and adjuvant chemotherapy at first relapse. At second relapse, he had progressive disease postresection. He was treated with ifosfamide/carboplatin/etoposide chemotherapy followed by maintenance irinotecan and achieved long-term disease-free survival. Irinotecan as maintenance should be explored in recurrent hepatoblastoma. (Source: Journal of Pediatric Hematology Oncology)
Source: Journal of Pediatric Hematology Oncology - September 29, 2020 Category: Hematology Tags: Online Articles: Clinical and Laboratory Observations Source Type: research

Chordoma of the Clivus and Acute Myeloid Leukemia: Is There a Connection?
We present a case of acute myeloid leukemia developing during treatment of localized chordoma of the clivus in a 20-month-old male. We propose a genomic relationship that may have contributed to the development of clival chordoma and acute myeloid leukemia without a latency period and advocate for genomic sequencing in children with chordoma before the initiation of systemic therapies. (Source: Journal of Pediatric Hematology Oncology)
Source: Journal of Pediatric Hematology Oncology - September 29, 2020 Category: Hematology Tags: Online Articles: Clinical and Laboratory Observations Source Type: research

Dasatinib-induced Chylothorax in Chronic Myelogenous Leukemia in Pediatric Patient: Report of a Case and Review of Literature
Dasatinib is a second-generation potent and efficacious oral tyrosine kinase inhibitor frequently used for imatinib-resistant or intolerant BCR-ABL–positive chronic myeloid leukemia and for Philadelphia chromosome–positive acute lymphocytic leukemia. Dasatinib is known to cause adverse pulmonary events such as chylothorax and has been described in the adult literature but not pediatric literature. The authors present a pediatric case of dasatinib-related chylothorax, subsequent management, and a review of the literature of adult cases with dasatinib-related chylothorax. (Source: Journal of Pediatric Hematology Oncology)
Source: Journal of Pediatric Hematology Oncology - September 29, 2020 Category: Hematology Tags: Online Articles: Clinical and Laboratory Observations Source Type: research

Ectopic ACTH Syndrome in a Child With Abdominal Ewing Sarcoma: A Rare Paraneoplastic Phenomenon
We report a 15-year-old boy with abdominal Ewing sarcoma and clinical and laboratory findings of ectopic adrenocorticotropic hormone syndrome that promptly resolved with treatment of the tumor. (Source: Journal of Pediatric Hematology Oncology)
Source: Journal of Pediatric Hematology Oncology - September 29, 2020 Category: Hematology Tags: Online Articles: Clinical and Laboratory Observations Source Type: research

Hematogone Hyperplasia Masquerading as Acute Lymphoblastic Leukemia With Concurrent Hypercupremia
Alterations in copper homeostasis is an uncommon cause for hematologic alterations frequently presenting with dysplastic features in the bone marrow. Most of these alterations have been documented in adult patients with copper deficiency. Rare cases show hematogone hyperplasia in these patients. Effects of mild copper excess have not been documented in literature. We are describing a pediatric patient who presented with pancytopenia associated with hypercupraemia (excess of copper). Bone marrow examination showed hematogone hyperplasia. Interestingly, correction of serum copper levels with zinc therapy lead to complete imp...
Source: Journal of Pediatric Hematology Oncology - September 29, 2020 Category: Hematology Tags: Online Articles: Clinical and Laboratory Observations Source Type: research

Diffuse Midline Glioma With Osseous Metastases at Diagnosis: A Case Report
Extraneural metastasis is extremely rare in pediatric patients with high-grade glioma and carries a grim prognosis. Detection of metastases at initial presentation is even rarer. A 15-year-old adolescent girl presented with paraplegia, urinary retention, and a constellation of systemic symptoms. Imaging showed a fourth ventricular lesion, innumerable intradural lesions, leptomeningeal seeding throughout the neuraxis, and numerous osteoblastic lesions involving the spine, ribs, sternum, pelvis, humerus, and femurs. Pathology confirmed metastatic diffuse midline glioma, H3K27M-mutant. Our patient died 2 weeks after initial p...
Source: Journal of Pediatric Hematology Oncology - September 29, 2020 Category: Hematology Tags: Online Articles: Clinical and Laboratory Observations Source Type: research

A Case of Pulmonary Veno-occlusive Disease Following Hepatic Veno-occlusive Disease After Autologous Hematopoietic Stem Cell Transplantation for Neuroblastoma
Pulmonary veno-occlusive disease (PVOD) is an uncommon form of pulmonary hypertension that is usually difficult to diagnose and is refractory to conservative treatment. PVOD can occur in connection with high-dose chemotherapy or hematopoietic stem cell transplantation, similar to hepatic veno-occlusive disease (HVOD). Here, we present a case of neuroblastoma with PVOD following HVOD after high-dose chemotherapy that was resolved with conservative treatment. Respiratory symptoms or edema after HVOD may suggest PVOD, and prompt diagnosis on high-resolution computed tomography will result in a favorable prognosis. (Source: Jo...
Source: Journal of Pediatric Hematology Oncology - September 29, 2020 Category: Hematology Tags: Online Articles: Clinical and Laboratory Observations Source Type: research

The Successful Sequential Use of Plerixafor and Eltrombopag For Hematopoietic Cell Transplantation in a Child With High-risk Neuroblastoma
We describe a case of a child with high-risk neuroblastoma, for whom plerixafor and eltrombopag were used as an effective and safe supportive therapy. (Source: Journal of Pediatric Hematology Oncology)
Source: Journal of Pediatric Hematology Oncology - September 29, 2020 Category: Hematology Tags: Online Articles: Clinical and Laboratory Observations Source Type: research

Percutaneous Drainage of an Iliopsoas Hematoma in a Hemophilia Patient
In patients with hemophilia, the usual treatment for both iliopsoas hematoma and associated femoral nerve compression is conservative. The case presented herein is a moderate hemophilia A patient with significant femoral neuropathy due to iliopsoas hematoma whose symptoms could not be managed with an aggressive factor VIII replacement program for>2 weeks. An unusual treatment option - percutaneous catheter insertion and aspiration of the hematoma - alleviated the examination findings and reversed his abilities. (Source: Journal of Pediatric Hematology Oncology)
Source: Journal of Pediatric Hematology Oncology - September 29, 2020 Category: Hematology Tags: Online Articles: Clinical and Laboratory Observations Source Type: research

Beta-Spectrin Deletion Responsible for Hereditary Spherocytosis: When New Technologies Are Not the Key to Success
We report the case of a boy with spherocytic anemia and development delay in whom a de novo 2.84-Mb deletion at chromosome 14 including SPTB (ß-spectrin gene) was identified by array-based comparative genomic hybridization. This alteration, consistent with de novo spherocytosis, was missed by a NGS gene panel. When associated with other symptoms, especially neurologic, NGS may not be appropriate to genetically diagnose spherocytic anemia. (Source: Journal of Pediatric Hematology Oncology)
Source: Journal of Pediatric Hematology Oncology - September 29, 2020 Category: Hematology Tags: Online Articles: Clinical and Laboratory Observations Source Type: research

Constitutional Mismatch Repair Gene Defect Syndrome Presenting With Adenomatous Polyposis and Cafe au Lait Spots: A Case Report
Conclusions: Adenomatous polyps are very important in childhood because of rarity. In particular, the presence of cafe au lait spots and a history of malignancy detected in relatives at an early age must be considered for CMMRD. (Source: Journal of Pediatric Hematology Oncology)
Source: Journal of Pediatric Hematology Oncology - September 29, 2020 Category: Hematology Tags: Online Articles: Clinical and Laboratory Observations Source Type: research

Successful Stem Cell Apheresis Using Spectra Optia in a 6 kg Child With Atypical Teratoid/Rhabdoid Tumor
Peripheral blood stem cell apheresis has become a routine procedure for the collection of peripheral blood stem cells to enable high-dose chemotherapy followed by autologous stem cell transplantation in high-risk pediatric malignancies. However, the procedure remains challenging in very low-weight infants due to high extracorporeal blood volume and citrate toxicity. Our case report demonstrates in detail a successful and complication-free large-volume leukapheresis in a very small infant weighing 6 kg using a Spectra Optia apheresis system after placing a femoral double-lumen Shaldon catheter. Anticoagulation was ac...
Source: Journal of Pediatric Hematology Oncology - September 29, 2020 Category: Hematology Tags: Online Articles: Clinical and Laboratory Observations Source Type: research

Congenital Hemolytic Anemia Because of Glucose Phosphate Isomerase Deficiency: Identification of 2 Novel Missense Mutations in the GPI Gene
We report a girl with severe hemolytic anemia at birth because of GPI deficiency. Enzyme activity assays were inconclusive because of previous blood transfusions. She was found to be compound heterozygous for 2 novel missense mutations, c.490C>A p.(Pro164Thr) and c.817C>T p.(Arg273Cys), in the GPI gene. Other than the chronic hemolytic anemia, she also has mild fine motor, gross motor delay, and developed cerebella ataxia since 5 years old. (Source: Journal of Pediatric Hematology Oncology)
Source: Journal of Pediatric Hematology Oncology - September 29, 2020 Category: Hematology Tags: Online Articles: Clinical and Laboratory Observations Source Type: research

Is Hemoglobin D Trait Hematologically Silent: Comparison With Healthy Controls and β-thalassemia Carriers
Hemoglobin D-Los Angeles, a recessively inherited hemoglobin variant, has been introduced as hematologically silent in the heterozygous state. However, as its compound heterozygosity with other hemoglobinopathies may lead to a severe clinical phenotype, with hemoglobin S being the most serious, the detection of carriers is important. To clarify the hematologic picture, we assessed erythrocyte parameters in D carriers and compared values in healthy controls and β-thalassemia carriers. Although values in D carriers, in the absence of confounding factors, significantly differed from thalassemia carriers (P (Source: Journ...
Source: Journal of Pediatric Hematology Oncology - September 29, 2020 Category: Hematology Tags: Online Articles: Clinical and Laboratory Observations Source Type: research

Efficacy, Retention And Safety Of Tofacitinib In Real-Life: Hur-Bio Monocentric Experience.
CONCLUSION: In conclusion, tofacitinib is an effective -as monotherapy or combination with csDMARDs- and well-tolerated treatment option in Turkish RA patients. PMID: 32979899 [PubMed - as supplied by publisher] (Source: Turkish Journal of Medical Sciences)
Source: Turkish Journal of Medical Sciences - September 29, 2020 Category: General Medicine Tags: Turk J Med Sci Source Type: research

Non-diabetic glycosuria as a diagnostic clue for acute tubulointerstitial nephritis in patients with azotemia.
CONCLUSION: In non-diabetic, non-nephrotic patients undergoing a kidney biopsy for azotemia, 1+ or higher glycosuria, if present, was a good predictor of the diagnosis of ATIN. PMID: 32985319 [PubMed - in process] (Source: Renal Failure)
Source: Renal Failure - September 29, 2020 Category: Urology & Nephrology Tags: Ren Fail Source Type: research

Effect of gabapentin on sleep-deprivation-induced disruption of prepulse inhibition
ConclusionsSleep deprivation via modified multiple platform technique could be used as an animal model for psychosis. Gabapentin may have dose- and duration-dependent effects on sensorimotor gating and locomotor activity. (Source: Psychopharmacology)
Source: Psychopharmacology - September 29, 2020 Category: Psychiatry Source Type: research

Comparison of two methods for the assessment of intra-erythrocyte magnesium and its determinants: Results from the LifeLines cohort study
Publication date: November 2020Source: Clinica Chimica Acta, Volume 510Author(s): Joëlle C. Schutten, Adrian Post, Margriet van der Meer, Jan IJmker, Frans Goorman, Richard M. Danel, Marc G. Vervloet, Martin H. de Borst, Daan J. Touw, Stephan J.L. Bakker (Source: Clinica Chimica Acta)
Source: Clinica Chimica Acta - September 29, 2020 Category: Laboratory Medicine Source Type: research

Comparison of clinical characteristics between coronavirus disease 2019 pneumonia and community - acquired pneumonia.
This study aimed to compare clinical features in adult patients with coronavirus disease 2019 (COVID-19) pneumonia to those in adult patients with community-acquired pneumonia (CAP).Methods: Clinical presentations, laboratory findings, imaging features, complications, treatment and outcomes were compared between patients with COVID-19 pneumonia and patients with CAP. The study group of patients with COVID-19 pneumonia consisted of 120 patients. One hundred and thirty-four patients with CAP were enrolled for comparison.Results: Patients with COVID-19 pneumonia had lower levels of abnormal laboratory parameters [white blood ...
Source: Current Medical Research and Opinion - September 29, 2020 Category: Research Tags: Curr Med Res Opin Source Type: research

High Sensitivity Troponin-T for Prediction of Adverse Events in Patients with COVID-19.
Authors: Singh N, Anchan RK, Besser SA, Belkin MN, Dela Cruz M, Lee L, Yu D, Mehta N, Nguyen AB, Alenghat FJ Abstract Background: High sensitivity troponin-T (hs-TnT) has been associated with mortality in patients hospitalized with COVID-19. We aimed to determine if hs-TnT levels and their timing are independent predictors of adverse events in these patients.Design: Retrospective chart review was performed for all patients hospitalized at our institution between March 23rd and April 13th, 2020 who were found to be COVID-19-positive. Clinical, demographic, and laboratory variables including initial and peak hs-TnT w...
Source: Biomarkers - September 29, 2020 Category: Research Tags: Biomarkers Source Type: research

Bonding crowns and bridges with resin cement: An American Dental Association Clinical Evaluators Panel survey.
CONCLUSIONS: The types of resin cements used, tooth preparation, crown or bridge preparation, and bonding technique vary among this sample. PRACTICAL IMPLICATIONS: Although many dentists bond crowns and bridges on the basis of best practices, improvement in the process may be achieved by dentists communicating with their laboratory to confirm the steps performed there, ensuring an effective cleaning technique is used after try-in and verifying that the correct primer is used with their chosen restorative material. PMID: 32979959 [PubMed - in process] (Source: Journal of the American Dental Association)
Source: Journal of the American Dental Association - September 29, 2020 Category: Dentistry Tags: J Am Dent Assoc Source Type: research

Diagnostic accuracy of 3 urine lipoarabinomannan tuberculosis assays in HIV-negative outpatients
CONCLUSION Compared with AlereLAM, FujiLAM detected 5 times more patients with TB in HIV-negative participants, had a high positive predictive value, and has the potential to improve rapid diagnosis of TB at the point-of-care. EclLAM demonstrated that additional sensitivity gains are possible, which highlights LAM’s potential as a biomarker. Additional research is required to assess FujiLAM’s performance in prospective cohorts, its cost-effectiveness, and its impact in real-world clinical settings.FUNDING Global Health Innovative Technology Fund, the UK Department for International Development, the Dutch Minist...
Source: Journal of Clinical Investigation - September 29, 2020 Category: Biomedical Science Authors: Tobias Broger, Mark P. Nicol, George B. Sigal, Eduardo Gotuzzo, Alexandra J. Zimmer, Shireen Surtie, Tatiana Caceres-Nakiche, Anna Mantsoki, Elena Ivanova Reipold, Rita Székely, Michael Tsionsky, Judith van Heerden, Tatiana Plisova, Kinuyo Chikamatsu, To Source Type: research

A Time Frame for Testing Negative for SARS-COV2 in People with Obesity
Conclusions: Our results highlight the need for priority of early detection and testing, and early therapy for people with obesity and COVID-19 infections.Obes Facts (Source: Obesity Facts)
Source: Obesity Facts - September 29, 2020 Category: Eating Disorders & Weight Management Source Type: research

A statistically rigorous deep neural network approach to predict mortality in trauma patients admitted to the intensive care unit
CONCLUSION Our novel Deep-FLAIM model outperformed all other machine learning models. The model is easy to implement, user friendly and with high accuracy. LEVEL OF EVIDENCE Prognostic study, level II. (Source: Journal of Trauma and Acute Care Surgery)
Source: Journal of Trauma and Acute Care Surgery - September 29, 2020 Category: Surgery Tags: ORIGINAL ARTICLES Source Type: research

Health Disparities Among Hispanics With Rheumatoid Arthritis: Delay in Presentation to Rheumatologists Contributes to Later Diagnosis and Treatment
Conclusions There is significant delay in initial presentation to a rheumatologist that was associated with a higher disease severity at presentation, especially for Hispanic patients. (Source: JCR: Journal of Clinical Rheumatology)
Source: JCR: Journal of Clinical Rheumatology - September 29, 2020 Category: Rheumatology Tags: Original Articles Source Type: research

Optically Based Hand-Held Sensor for Visualization and Quantification of Cryptosporidium Parvum
AbstractThe ingestion of the protozoan parasiteCryptosporidium, resulting in the associated disease cryptosporidiosis, can cause major problems if introduced into a water supply for both animals and humans. This research focuses on the creation of a sensor to track and quantify the parasite with a digital camera system using Digital Inline Holographic Microscopy (DIHM) and software reconstruction. SinceCryptosporidium oocysts are not single celled and can be around 4 –8 µm in diameter, they are easily detected with the DIHM at various depths within a 3.5 mL cuvette. As the sensor is improved by modifi...
Source: Sensing and Imaging - September 29, 2020 Category: Biomedical Engineering Source Type: research

Using affinity propagation clustering for identifying bacterial clades and subclades with whole-genome sequences of < i > Francisella tularensis < /i >
by Anne Busch, Timo Homeier-Bachmann, Mostafa Y. Abdel-Glil, Anja Hackbart, Helmut Hotzel, Herbert Tomaso By combining a reference-independent SNP analysis and average nucleotide identity (ANI) with affinity propagation clustering (APC), we developed a significantly improved methodology allowing resolving phylogenetic relationships, based on objective criteria. These bioinformatics tools can be used a s a general ruler to determine phylogenetic relationships and clustering of bacteria, exemplary done withFrancisella (F.)tularensis. Molecular epidemiology ofF.tularensis is currently assessed mostly based on laboratory meth...
Source: PLoS Neglected Tropical Diseases - September 29, 2020 Category: Tropical Medicine Authors: Anne Busch Source Type: research

Obstetric Hemorrhage Risk Associated with Novel COVID-19 Diagnosis from a Single-Institution Cohort in the United States
Am J Perinatol DOI: 10.1055/s-0040-1718403 Objective The study aimed to compare the quantitative blood loss (QBL) and hemorrhage-related outcomes of pregnant women with and without a coronavirus disease 2019 (COVID-19) diagnosis. Study Design This retrospective cohort study of all live deliveries at Boston Medical Center between April 1, 2020 and July 22, 2020 compares the outcomes of pregnant women with a laboratory-confirmed COVID-19 positive diagnosis and pregnant women without COVID-19. The primary outcomes are QBL and obstetric hemorrhage. The secondary outcomes analyzed were a maternal composite outco...
Source: American Journal of Perinatology - September 29, 2020 Category: Perinatology & Neonatology Authors: Wang, Michelle J. Schapero, Melissa Iverson, Ronald Yarrington, Christina D. Tags: Original Article Source Type: research

IJERPH, Vol. 17, Pages 7132: HealthyBlock: Blockchain-Based IT Architecture for Electronic Medical Records Resilient to Connectivity Failures
rris Pedro Wightman The current information systems for the registration and control of electronic medical records (EMR) present a series of problems in terms of the fragmentation, security, and privacy of medical information, since each health institution, laboratory, doctor, etc. has its own database and manages its own information, without the intervention of patients. This situation does not favor effective treatment and prevention of diseases for the population, due to potential information loss, misinformation, or data leaks related to a patient, which in turn may imply a direct risk for the individual and high...
Source: International Journal of Environmental Research and Public Health - September 29, 2020 Category: Environmental Health Authors: Omar Guti érrez Giordy Romero Luis P érez Augusto Salazar Marina Charris Pedro Wightman Tags: Article Source Type: research

[ 18 F]MPPF and [ 18 F]FDG μPET imaging in rats: impact of transport and restraint stress
ConclusionsMild-to-moderate stress associated with daily transport and exposure to a laboratory environment can trigger significant alterations in hippocampal binding of the 5-HT1A receptor ligand [18F]MPPF. This finding indicates that utmost care is necessary to control and report transport and associated handling procedures for animals used in μPET studies analyzing the serotonergic system in order to enhance the robustness of conclusions and allow replicability of findings. In view of earlier studies indicating that an increase in hippocampal 5-HT1A receptor expression may be associated with a resilience to stress, i...
Source: EJNMMI Research - September 29, 2020 Category: Radiology Source Type: research

Test of the auricular surface sex estimation method in fetuses and non-adults under 5  years old from the Lisbon and Granada Reference Collections
The objectives of this study are threefold: to test the validity of the auricular surface method for sex estimation (Int J Osteoarchaeol 27:898 –911,2017) in fetuses and children under the age of 5  years old; to evaluate if health conditions, reported as the cause of death, influence its accuracy; and to detect possible secular trends in sexual dimorphism. One-hundred and ninety-seven skeletal individuals from the Lisbon and Granada Identified Collections were studied. Individuals were divid ed according to the hormonal peaks (
Source: International Journal of Legal Medicine - September 29, 2020 Category: Medical Law Source Type: research

Adrenal Tumor Mimicking Non-Classic Congenital Adrenal Hyperplasia
Elevated 17-hydroxyprogesterone may be caused by congenital adrenal hyperplasia, ovarian or adrenal tumors. A positive cosyntropin stimulation test result for 17-hydroxyprogesterone may be found in functional or non-functional tumors and be related to tumor size. Here, we present a case of a 36-year-old woman with a 4-year history of infertility. Laboratory test results revealed elevated progesterone and 17-hydroxyprogesterone, with normal luteinizing hormone, follicle-stimulating hormone, estrogen, testosterone, and anti-Mullerian hormone levels. The 250-μg cosyntropin stimulation test revealed a 17-hydroxyprogesterone...
Source: Frontiers in Endocrinology - September 29, 2020 Category: Endocrinology Source Type: research

Higher Muscle Mass Implies Increased Free-Thyroxine to Free-Triiodothyronine Ratio in Subjects With Overweight and Obesity
In conclusion, preserving a greater skeletal MM in obesity helps to enhance thyroid activity.Clinical Trial RegistrationClinicalTrials.gov, identifier NCT04327375. (Source: Frontiers in Endocrinology)
Source: Frontiers in Endocrinology - September 29, 2020 Category: Endocrinology Source Type: research

An Atypical Presentation of Primary Hyperparathyroidism in an Adolescent: A Case Report of Hypercalcaemia and Neuropsychiatric Symptoms Due to a Mediastinal Parathyroid Adenoma
We described the case of a 17-year-old girl affected by anorexia nervosa, depression and self-harm with incidental detection of moderate hypercalcaemia. Clinical, laboratory and instrumental tests demonstrated that hypercalcaemia was secondary to primary hyperparathyroidism (PHPT) due to a mediastinal parathyroid adenoma in the thymic parenchyma. After parathyroidectomy with robot-assisted surgery, we observed the restoration of calcium and PTH levels in addition to an improvement in psychiatric symptoms. This case demonstrates that serum calcium concentration should be evaluated in adolescents with neurobehavioural sympto...
Source: Frontiers in Endocrinology - September 29, 2020 Category: Endocrinology Source Type: research

Renal pseudotumor: A new challenge in the diagnosis of immunoglobulin G4-related disease
Conclusion: We should consider pseudotumor-like IgG4-RD as a differential diagnosis for solitary renal lesion although the incidence is low. (Source: Journal of Cancer Research and Therapeutics)
Source: Journal of Cancer Research and Therapeutics - September 29, 2020 Category: Cancer & Oncology Authors: Qian Wu Na Zhao Xia Wang Hongsheng Sun Limin Zhang Source Type: research

Association of high HIF-1 α levels in serous periodontitis with external root resorption by the NFATc1 pathway
AbstractWhether external root resorption is associated with hypoxia in the periodontal ligaments of teeth with severe periodontitis remains unclear. Hypoxia inducible factor-1 α (HIF-1α) expression and external resorption sites in the periodontal ligaments of these teeth were observed to elaborate upon the relationship between hypoxia and external root resorption in severe periodontitis. Histological analysis was performed to observe external root resorption. The expres sions of HIF-1α and Nuclear factor-activated T cells c1 (NFATc1) in the periodontal ligaments were detected by immunofluorescence, wester...
Source: Journal of Molecular Histology - September 29, 2020 Category: Laboratory Medicine Source Type: research

Viruses, Vol. 12, Pages 1102: Treatment with Exogenous Trypsin Expands In Vitro Cellular Tropism of the Avian Coronavirus Infectious Bronchitis Virus
This study aims to investigate whether protease treatment can extend the tropism of other IBV strains. Here we demonstrate that the addition of exogenous trypsin during IBV propagation in cell culture results in significantly increased viral titres. Using a panel of IBV strains, exhibiting varied tropisms, the effects of spike cleavage on entry and replication were assessed by serial passage cell culture in the presence of trypsin. Replication could be maintained over serial passages, indicating that the addition of exogenous protease is sufficient to overcome the barrier to infection. Mutations were identified in both S1 ...
Source: Viruses - September 29, 2020 Category: Virology Authors: Phoebe Stevenson-Leggett Sarah Keep Erica Bickerton Tags: Article Source Type: research

Integration of Wet-Lab Measures, Milk Infrared Spectra, and Genomics to Improve Difficult-to-Measure Traits in Dairy Cattle Populations
This study suggests that breeding programs for difficult-to-measure traits could be implemented using FTIR information. While these programs should use progeny testing, acceptable values of accuracy can be achieved also for bulls without phenotyped progeny. Robust calibration equations are, deemed as essential. (Source: Frontiers in Genetics)
Source: Frontiers in Genetics - September 29, 2020 Category: Genetics & Stem Cells Source Type: research