Chronic < em > Toxoplasma < /em > infection affects gene expression of drug-metabolizing enzymes in mouse liver
Xenobiotica. 2023 Nov 22:1-11. doi: 10.1080/00498254.2023.2286597. Online ahead of print.ABSTRACTToxoplasma gondii is an intracellular protozoan parasite causing toxoplasmosis, an infectious disease affecting warm-blooded vertebrates worldwide. Many drug-metabolizing enzymes are located in the liver, a major organ of drug metabolism, and their function can be affected by pathogen infection.Using next-generation sequencing (RNA-seq) and quantitative polymerase chain reaction (qPCR), changes in the hepatic expressions of drug-metabolizing enzymes were analyzed in mice chronically infected with T. gondii. The analysis found t...
Source: Xenobiotica - November 22, 2023 Category: Research Authors: Yasuhiro Uno Shotaro Uehara Genki Ushirozako Tatsunori Masatani Hiroshi Yamazaki Source Type: research
Population pharmacokinetics of mycophenolic acid and dose optimization in adult Chinese kidney transplant recipients
This study aimed to establish a population pharmacokinetic (PPK) model of mycophenolic acid (MPA), quantify the effect of clinical factors and pharmacogenomics of MPA, and optimize the dosage for adult kidney transplant recipients.2. One-hundred and four adult renal transplant patients were enrolled. The PPK model was established using the Phoenix® NMLE software and the stepwise methods were filtered for significant covariates. Monte Carlo simulations were performed to optimize the dosage regimen.3. A two-compartment model with first-order absorption and elimination (including lag time) provided a more accurate descriptio...
Source: Xenobiotica - November 22, 2023 Category: Research Authors: Kaisaner Rexiti Xuehui Jiang Ying Kong Xu Chen Hong Liu Hongwei Peng Xiaohua Wei Source Type: research
Chronic < em > Toxoplasma < /em > infection affects gene expression of drug-metabolizing enzymes in mouse liver
Xenobiotica. 2023 Nov 22:1-11. doi: 10.1080/00498254.2023.2286597. Online ahead of print.ABSTRACTToxoplasma gondii is an intracellular protozoan parasite causing toxoplasmosis, an infectious disease affecting warm-blooded vertebrates worldwide. Many drug-metabolizing enzymes are located in the liver, a major organ of drug metabolism, and their function can be affected by pathogen infection.Using next-generation sequencing (RNA-seq) and quantitative polymerase chain reaction (qPCR), changes in the hepatic expressions of drug-metabolizing enzymes were analyzed in mice chronically infected with T. gondii. The analysis found t...
Source: Xenobiotica - November 22, 2023 Category: Research Authors: Yasuhiro Uno Shotaro Uehara Genki Ushirozako Tatsunori Masatani Hiroshi Yamazaki Source Type: research
Population pharmacokinetics of mycophenolic acid and dose optimization in adult Chinese kidney transplant recipients
This study aimed to establish a population pharmacokinetic (PPK) model of mycophenolic acid (MPA), quantify the effect of clinical factors and pharmacogenomics of MPA, and optimize the dosage for adult kidney transplant recipients.2. One-hundred and four adult renal transplant patients were enrolled. The PPK model was established using the Phoenix® NMLE software and the stepwise methods were filtered for significant covariates. Monte Carlo simulations were performed to optimize the dosage regimen.3. A two-compartment model with first-order absorption and elimination (including lag time) provided a more accurate descriptio...
Source: Xenobiotica - November 22, 2023 Category: Research Authors: Kaisaner Rexiti Xuehui Jiang Ying Kong Xu Chen Hong Liu Hongwei Peng Xiaohua Wei Source Type: research
Chronic < em > Toxoplasma < /em > infection affects gene expression of drug-metabolizing enzymes in mouse liver
Xenobiotica. 2023 Nov 22:1-11. doi: 10.1080/00498254.2023.2286597. Online ahead of print.ABSTRACTToxoplasma gondii is an intracellular protozoan parasite causing toxoplasmosis, an infectious disease affecting warm-blooded vertebrates worldwide. Many drug-metabolizing enzymes are located in the liver, a major organ of drug metabolism, and their function can be affected by pathogen infection.Using next-generation sequencing (RNA-seq) and quantitative polymerase chain reaction (qPCR), changes in the hepatic expressions of drug-metabolizing enzymes were analyzed in mice chronically infected with T. gondii. The analysis found t...
Source: Xenobiotica - November 22, 2023 Category: Research Authors: Yasuhiro Uno Shotaro Uehara Genki Ushirozako Tatsunori Masatani Hiroshi Yamazaki Source Type: research
Population pharmacokinetics of mycophenolic acid and dose optimization in adult Chinese kidney transplant recipients
This study aimed to establish a population pharmacokinetic (PPK) model of mycophenolic acid (MPA), quantify the effect of clinical factors and pharmacogenomics of MPA, and optimize the dosage for adult kidney transplant recipients.2. One-hundred and four adult renal transplant patients were enrolled. The PPK model was established using the Phoenix® NMLE software and the stepwise methods were filtered for significant covariates. Monte Carlo simulations were performed to optimize the dosage regimen.3. A two-compartment model with first-order absorption and elimination (including lag time) provided a more accurate descriptio...
Source: Xenobiotica - November 22, 2023 Category: Research Authors: Kaisaner Rexiti Xuehui Jiang Ying Kong Xu Chen Hong Liu Hongwei Peng Xiaohua Wei Source Type: research
Investigation of pharmacokinetic properties of a PEGylated bilirubin nanoparticle in male Sprague-Dawley rats using liquid chromatography-quadrupole time-of-flight mass spectrometry
Xenobiotica. 2023 Nov 16:1-36. doi: 10.1080/00498254.2023.2284859. Online ahead of print.ABSTRACTPolyethylene glycol (PEG) was introduced into synthetic bilirubin 3α and a PEGylated bilirubin 3α nanoparticle (BX-001N, Brixelle®) was developed for the first time.An in vitro microsomal stability study, in vivo PK studies with intravenous bolus (IV) and subcutaneous injection (SC), and a semi-mass balance study of BX-001N were investigated to evaluate its pharmacokinetic (PK) properties in male Sprague-Dawley (SD) rats using developed liquid chromatography-quadrupole time-of-flight mass spectrometry (LC-qTOF/MS).Following ...
Source: Xenobiotica - November 16, 2023 Category: Research Authors: Seo-Jin Park Jeong-Hyeon Lim Jiyu Lee Jeongmin Lee Sangsoo Hwang Hyunjin Kim Seunghyun Jo Duckhyang Shin Sang Ho Ma Myung L Kim Young G Shin Source Type: research
Study on the transport and internalization mechanism of dietary supplement nattokinase in the small intestine using animal and Caco-2 cell monolayer models
This study first evaluated the preventive effect of supplementing low dose (4000 FU (Fibrin Unit)/kg, n = 6), medium dose (8000 FU/kg, n = 6), and high dose (12000 FU/kg, n = 6) of nattokinase on carrageenan induced thrombosis in mice. Subsequently, we used the rat gut sac model, ligated intestinal loop model, and Caco-2 cell uptake model to study the intestinal transport mechanism of NK.4. Results indicate that NK is a moderately absorbed biomolecule whose transport through enterocytes is energy- and time-dependent. Chlorpromazine, nystatin and EIPA all inhibited the endocytosis of NK to varying degrees, indicating that t...
Source: Xenobiotica - November 16, 2023 Category: Research Authors: Huawei Feng Chang Liu Qingqing Liu Jie Wang Yingyue Zeng Yue Sun Man Zhang Hui Zhang Zhikui Liu Jian Zhao Hongsheng Liu Source Type: research
Investigation of pharmacokinetic properties of a PEGylated bilirubin nanoparticle in male Sprague-Dawley rats using liquid chromatography-quadrupole time-of-flight mass spectrometry
Xenobiotica. 2023 Nov 16:1-36. doi: 10.1080/00498254.2023.2284859. Online ahead of print.ABSTRACTPolyethylene glycol (PEG) was introduced into synthetic bilirubin 3α and a PEGylated bilirubin 3α nanoparticle (BX-001N, Brixelle®) was developed for the first time.An in vitro microsomal stability study, in vivo PK studies with intravenous bolus (IV) and subcutaneous injection (SC), and a semi-mass balance study of BX-001N were investigated to evaluate its pharmacokinetic (PK) properties in male Sprague-Dawley (SD) rats using developed liquid chromatography-quadrupole time-of-flight mass spectrometry (LC-qTOF/MS).Following ...
Source: Xenobiotica - November 16, 2023 Category: Research Authors: Seo-Jin Park Jeong-Hyeon Lim Jiyu Lee Jeongmin Lee Sangsoo Hwang Hyunjin Kim Seunghyun Jo Duckhyang Shin Sang Ho Ma Myung L Kim Young G Shin Source Type: research
Study on the transport and internalization mechanism of dietary supplement nattokinase in the small intestine using animal and Caco-2 cell monolayer models
This study first evaluated the preventive effect of supplementing low dose (4000 FU (Fibrin Unit)/kg, n = 6), medium dose (8000 FU/kg, n = 6), and high dose (12000 FU/kg, n = 6) of nattokinase on carrageenan induced thrombosis in mice. Subsequently, we used the rat gut sac model, ligated intestinal loop model, and Caco-2 cell uptake model to study the intestinal transport mechanism of NK.4. Results indicate that NK is a moderately absorbed biomolecule whose transport through enterocytes is energy- and time-dependent. Chlorpromazine, nystatin and EIPA all inhibited the endocytosis of NK to varying degrees, indicating that t...
Source: Xenobiotica - November 16, 2023 Category: Research Authors: Huawei Feng Chang Liu Qingqing Liu Jie Wang Yingyue Zeng Yue Sun Man Zhang Hui Zhang Zhikui Liu Jian Zhao Hongsheng Liu Source Type: research
Investigation of pharmacokinetic properties of a PEGylated bilirubin nanoparticle in male Sprague-Dawley rats using liquid chromatography-quadrupole time-of-flight mass spectrometry
Xenobiotica. 2023 Nov 16:1-36. doi: 10.1080/00498254.2023.2284859. Online ahead of print.ABSTRACTPolyethylene glycol (PEG) was introduced into synthetic bilirubin 3α and a PEGylated bilirubin 3α nanoparticle (BX-001N, Brixelle®) was developed for the first time.An in vitro microsomal stability study, in vivo PK studies with intravenous bolus (IV) and subcutaneous injection (SC), and a semi-mass balance study of BX-001N were investigated to evaluate its pharmacokinetic (PK) properties in male Sprague-Dawley (SD) rats using developed liquid chromatography-quadrupole time-of-flight mass spectrometry (LC-qTOF/MS).Following ...
Source: Xenobiotica - November 16, 2023 Category: Research Authors: Seo-Jin Park Jeong-Hyeon Lim Jiyu Lee Jeongmin Lee Sangsoo Hwang Hyunjin Kim Seunghyun Jo Duckhyang Shin Sang Ho Ma Myung L Kim Young G Shin Source Type: research
Study on the transport and internalization mechanism of dietary supplement nattokinase in the small intestine using animal and Caco-2 cell monolayer models
This study first evaluated the preventive effect of supplementing low dose (4000 FU (Fibrin Unit)/kg, n = 6), medium dose (8000 FU/kg, n = 6), and high dose (12000 FU/kg, n = 6) of nattokinase on carrageenan induced thrombosis in mice. Subsequently, we used the rat gut sac model, ligated intestinal loop model, and Caco-2 cell uptake model to study the intestinal transport mechanism of NK.4. Results indicate that NK is a moderately absorbed biomolecule whose transport through enterocytes is energy- and time-dependent. Chlorpromazine, nystatin and EIPA all inhibited the endocytosis of NK to varying degrees, indicating that t...
Source: Xenobiotica - November 16, 2023 Category: Research Authors: Huawei Feng Chang Liu Qingqing Liu Jie Wang Yingyue Zeng Yue Sun Man Zhang Hui Zhang Zhikui Liu Jian Zhao Hongsheng Liu Source Type: research
Investigation of pharmacokinetic properties of a PEGylated bilirubin nanoparticle in male Sprague-Dawley rats using liquid chromatography-quadrupole time-of-flight mass spectrometry
Xenobiotica. 2023 Nov 16:1-36. doi: 10.1080/00498254.2023.2284859. Online ahead of print.ABSTRACTPolyethylene glycol (PEG) was introduced into synthetic bilirubin 3α and a PEGylated bilirubin 3α nanoparticle (BX-001N, Brixelle®) was developed for the first time.An in vitro microsomal stability study, in vivo PK studies with intravenous bolus (IV) and subcutaneous injection (SC), and a semi-mass balance study of BX-001N were investigated to evaluate its pharmacokinetic (PK) properties in male Sprague-Dawley (SD) rats using developed liquid chromatography-quadrupole time-of-flight mass spectrometry (LC-qTOF/MS).Following ...
Source: Xenobiotica - November 16, 2023 Category: Research Authors: Seo-Jin Park Jeong-Hyeon Lim Jiyu Lee Jeongmin Lee Sangsoo Hwang Hyunjin Kim Seunghyun Jo Duckhyang Shin Sang Ho Ma Myung L Kim Young G Shin Source Type: research
Study on the transport and internalization mechanism of dietary supplement nattokinase in the small intestine using animal and Caco-2 cell monolayer models
This study first evaluated the preventive effect of supplementing low dose (4000 FU (Fibrin Unit)/kg, n = 6), medium dose (8000 FU/kg, n = 6), and high dose (12000 FU/kg, n = 6) of nattokinase on carrageenan induced thrombosis in mice. Subsequently, we used the rat gut sac model, ligated intestinal loop model, and Caco-2 cell uptake model to study the intestinal transport mechanism of NK.4. Results indicate that NK is a moderately absorbed biomolecule whose transport through enterocytes is energy- and time-dependent. Chlorpromazine, nystatin and EIPA all inhibited the endocytosis of NK to varying degrees, indicating that t...
Source: Xenobiotica - November 16, 2023 Category: Research Authors: Huawei Feng Chang Liu Qingqing Liu Jie Wang Yingyue Zeng Yue Sun Man Zhang Hui Zhang Zhikui Liu Jian Zhao Hongsheng Liu Source Type: research
Investigation of pharmacokinetic properties of a PEGylated bilirubin nanoparticle in male Sprague-Dawley rats using liquid chromatography-quadrupole time-of-flight mass spectrometry
Xenobiotica. 2023 Nov 16:1-36. doi: 10.1080/00498254.2023.2284859. Online ahead of print.ABSTRACTPolyethylene glycol (PEG) was introduced into synthetic bilirubin 3α and a PEGylated bilirubin 3α nanoparticle (BX-001N, Brixelle®) was developed for the first time.An in vitro microsomal stability study, in vivo PK studies with intravenous bolus (IV) and subcutaneous injection (SC), and a semi-mass balance study of BX-001N were investigated to evaluate its pharmacokinetic (PK) properties in male Sprague-Dawley (SD) rats using developed liquid chromatography-quadrupole time-of-flight mass spectrometry (LC-qTOF/MS).Following ...
Source: Xenobiotica - November 16, 2023 Category: Research Authors: Seo-Jin Park Jeong-Hyeon Lim Jiyu Lee Jeongmin Lee Sangsoo Hwang Hyunjin Kim Seunghyun Jo Duckhyang Shin Sang Ho Ma Myung L Kim Young G Shin Source Type: research
