Ultrastructural and morphometric alterations to the peripheral nerve following the administration of immunosuppressive agent tacrolimus (FK506)
Ultrastruct Pathol. 2021 Feb 17:1-6. doi: 10.1080/01913123.2021.1881673. Online ahead of print.ABSTRACTTacrolimus, a widely used immunosuppressive drug for preventing graft rejection following organ transplantation, was reported to develop neurotoxic side effects ranging from mild to severe symptoms in the literature. Rats were randomly divided into three groups as control and 2-week and 3-week treatment groups and received a 2 mg/kg/day tacrolimus by oral gavage. Animals were sacrificed and sciatic nerves obtained from all groups were fixed and processed for light and electron microscopic investigations. The myelinated fi...
Source: Ultrastructural Pathology - February 18, 2021 Category: Pathology Authors: Ferda Topal Celikkan Nazli Hayirli Ozyol Hilal Nakkas Oya Evirgen Source Type: research

Ultrastructural and morphometric alterations to the peripheral nerve following the administration of immunosuppressive agent tacrolimus (FK506)
Ultrastruct Pathol. 2021 Feb 17:1-6. doi: 10.1080/01913123.2021.1881673. Online ahead of print.ABSTRACTTacrolimus, a widely used immunosuppressive drug for preventing graft rejection following organ transplantation, was reported to develop neurotoxic side effects ranging from mild to severe symptoms in the literature. Rats were randomly divided into three groups as control and 2-week and 3-week treatment groups and received a 2 mg/kg/day tacrolimus by oral gavage. Animals were sacrificed and sciatic nerves obtained from all groups were fixed and processed for light and electron microscopic investigations. The myelinated fi...
Source: Ultrastructural Pathology - February 18, 2021 Category: Pathology Authors: Ferda Topal Celikkan Nazli Hayirli Ozyol Hilal Nakkas Oya Evirgen Source Type: research

Ultrastructural and morphometric alterations to the peripheral nerve following the administration of immunosuppressive agent tacrolimus (FK506).
Abstract Tacrolimus, a widely used immunosuppressive drug for preventing graft rejection following organ transplantation, was reported to develop neurotoxic side effects ranging from mild to severe symptoms in the literature. Rats were randomly divided into three groups as control and 2-week and 3-week treatment groups and received a 2 mg/kg/day tacrolimus by oral gavage. Animals were sacrificed and sciatic nerves obtained from all groups were fixed and processed for light and electron microscopic investigations. The myelinated fiber diameter, axon diameter, G-ratio (axon diameter/myelinated fiber diameter), and m...
Source: Ultrastructural Pathology - February 17, 2021 Category: Pathology Authors: Celikkan FT, Hayirli Ozyol N, Nakkas H, Evirgen O Tags: Ultrastruct Pathol Source Type: research

Ultrastructural and clinical features of central nervous system melanoma:Analysis of nine cases.
In this study, four stage II melanosomes were intracranial space-occupying, three of which were malignant melanoma, the other one was melanoma. Among the five stage IV melanosomes, four cases were intraspinal space-occupying, the other one was intracranial space-occupying, and the pathological diagnoses were all melanoma. At present, TEM is an important tool for the diagnosis of CNS melanomas. Malignant melanoma has high malignancy and recurrence rate and poor prognosis, while benign melanoma with relatively low recurrence rate, so we speculate that patients with mainly immature melanosomes are more likely to exhibit recur...
Source: Ultrastructural Pathology - February 15, 2021 Category: Pathology Authors: Zhang Q, Zhu H, Zhang C, Wang Y, He Y, Li G Tags: Ultrastruct Pathol Source Type: research

Deep learning for the classification of medical kidney disease: a pilot study for electron microscopy.
Abstract Artificial intelligence (AI) is a new frontier and often enigmatic for medical professionals. Cloud computing could open up the field of computer vision to a wider medical audience and deep learning on the cloud allows one to design, develop, train and deploy applications with ease. In the field of histopathology, the implementation of various applications in AI has been successful for whole slide images rich in biological diversity. However, the analysis of other tissue medias, including electron microscopy, is yet to be explored. The present study aims to evaluate deep learning for the classification of...
Source: Ultrastructural Pathology - February 14, 2021 Category: Pathology Authors: Hacking S, Bijol V Tags: Ultrastruct Pathol Source Type: research

Resveratrol ameliorates type 2 diabetes mellitus-induced alterations to the knee joint articular cartilage ultrastructure in rats.
Abstract Diabetes-induced osteoarthritis (OA) is a chronic inflammatory disease that damages the cartilage in the joints and could lead to disability. The protective effect of the antioxidant and anti-inflammatory agent, resveratrol, against alterations to the knee articular cartilage ultrastructure induced by type 2 diabetes mellitus (T2DM) associated with the inhibition of dyslipidemia, oxidative stress, and inflammation has not been investigated before. Therefore, we modeled OA in rats 10 weeks post diabetic induction using a high carbohydrate and fat diet and a single injection of streptozotocin (50 mg/kg...
Source: Ultrastructural Pathology - February 11, 2021 Category: Pathology Authors: El-Bidawy MH, Omar Hussain AB, Al-Ghamdi S, Aldossari KK, Haidara MA, Al-Ani B Tags: Ultrastruct Pathol Source Type: research

The combination of N-acetylcysteine and cyclosporin A reduces acetaminophen-induced hepatotoxicity in mice.
edil;ın T, Enver Ozan İ Abstract Acetaminophen (APAP)-induced hepatotoxicity is the most common cause of acute liver failure in worldwide. N-acetyl cysteine (NAC) is used as the APAP antidote. Cyclosporin A (CsA) is suppressed mitochondrial damage by binding cyclophilin, a mitochondrial pore transport component. The study aimed to evaluate the effects of NAC, CsA, and NAC+CsA treatments on APAP-induced hepatotoxicity in mice. Mice were randomly divided into five groups (n = 6). 400 mg/kg/ip/single dose APAP, 1200 mg/kg/i.p/single dose NAC and 50 mg/kg/i.p/single dose CsA were performed. Light and elect...
Source: Ultrastructural Pathology - February 2, 2021 Category: Pathology Authors: Kaya Tektemur N, Erdem Güzel E, Gül M, Tektemur A, Özcan Yıldırım S, Kavak Balgetir M, Ozan Kocamüftüoğlu G, Yalçın T, Enver Ozan İ Tags: Ultrastruct Pathol Source Type: research

A note from the Editor and Chief.
PMID: 33522365 [PubMed - as supplied by publisher] (Source: Ultrastructural Pathology)
Source: Ultrastructural Pathology - January 31, 2021 Category: Pathology Authors: Herrera G Tags: Ultrastruct Pathol Source Type: research

The effects of the anti-M üllerian hormone on folliculogenesis in rats: light and electron microscopic evaluation.
In this study, we evaluated the effects of anti-Müllerian hormone on follicle development and oocyte quality with light and electron microscopy. Twenty-four adult female rats were divided into four groups. After estrous cycle synchronization, on the first day, control group rats were injected with 0.5 ml saline, 2nd, 3rd, and 4th groups were injected 1 µgr, 2 µgr, and 5 µgr anti-Müllerian hormone, respectively. On the third day, intracardiac blood samples were taken for follicle-stimulating hormone, luteinizing hormone, estradiol, and progesterone serum level measurements. Ovaries were obta...
Source: Ultrastructural Pathology - January 24, 2021 Category: Pathology Authors: Kuyucu Y, Tap Ö Tags: Ultrastruct Pathol Source Type: research

Grape seed extract protects against amiodarone - induced nephrotoxicity and ultrastructural alterations associated with the inhibition of biomarkers of inflammation and oxidative stress in rats.
Abstract Amiodarone (AMD) is one of the highly effective antiarrhythmic agents used for treating refractory arrhythmias. It is well known to have long-term administration side effects such as nephrotoxicity. The possible ameliorative effects of antioxidant grape seed extract; on the extent of tissue damage in AMD-induced nephrotoxicity has not been investigated before. Twenty-four albino rats were used in this study and divided into four groups (n = 6). The 1st group served as an untreated control group, under the same laboratory conditions, the 2nd group received (100 mg/kg/day) of grape seed extract (G...
Source: Ultrastructural Pathology - January 11, 2021 Category: Pathology Authors: Eid RA, Zaki MSA, Al-Shraim M, Eldeen MA, Haidara MA Tags: Ultrastruct Pathol Source Type: research

The osmotic demyelination syndrome: the resilience of thalamic neurons is verified with transmission electron microscopy.
Abstract The development of a murine model of osmotic demyelinating syndrome (ODS) allowed to study changes incurred in extrapontine zones of the CNS and featured neuron and glial cell changes in the relay thalamic ventral posterolateral (VPL) and ventral posteromedial (VPM) nuclei before, during and after ODS induction, and characterized without immune response. There, the neuron Wallerian-type deteriorations were verified with fine structure modifications of the neuron cell body, including some nucleus topology and its nucleolus changes. Morphologic analyses showed a transient stoppage of transcriptional activit...
Source: Ultrastructural Pathology - January 3, 2021 Category: Pathology Authors: Gilloteaux J, Bouchat J, Brion JP, Nicaise C Tags: Ultrastruct Pathol Source Type: research

Effects of salubrinal on ER stress in an experimental model of polycystic ovary syndrome.
This study aimed to investigate the role of endoplasmic reticulum (ER) stress in polycystic ovary syndrome (PCOS) and the effect of salubrinal (SAL) on this role. Animals were divided into four groups as control, PCOS, PCOS+SAL and SAL. Weights and serum testosterone levels were increased in the PCOS group while serum LH and ATF4 expressions were decreased. Morphometrically, number of follicles with a diameter between 150 and 300 µm were declined and number of follicles larger than 300 µm as well as the percentage of cystic follicles (CFs) were increased. Immunoreactivities of GRP78 and p-eIF2α ...
Source: Ultrastructural Pathology - January 3, 2021 Category: Pathology Authors: Emincik A, Gungor ZB, Guzel E Tags: Ultrastruct Pathol Source Type: research

Perineurioma versus meningioma. A multi-institutional immunohistochemical and ultrastructural studY.
eida OPD, Lino-Silva LS Abstract The differential diagnosis between perineurioma (PN) and meningioma (MEN) can be difficult by histology and immunohistochemistry (IHC) because the perineurium and arachnoid have the same embryological origin. However, there are no comparative studies determining conclusive parameters for the differential diagnosis. The aim of this study is to compare IHC of PN and MEN and their ultra-structural characteristics to elucidate which are the useful data that allow differentiate both entities. Thirty-five MEN were analyzed, and 15 PN, (11 skin and soft tissues and four oral cavity)....
Source: Ultrastructural Pathology - December 15, 2020 Category: Pathology Authors: Domínguez-Malagón HR, Serrano-Arévalo ML, Maldonado J, Chávez J, Toussaint-Caire S, de Almeida OPD, Lino-Silva LS Tags: Ultrastruct Pathol Source Type: research

Electron microscopy in renal pathology: overall applications and guidelines for tissue, collection, preparation, and stains.
Abstract Electron microscopy is a mainstay in the analysis of renal biopsies, where it is typically employed in a correlative fashion along with light and immunofluorescence microscopy. Despite the development of a growing armamentarium of molecular and biochemical analytic methods as well as new immunostains with a widening panel of immunoreactants, electron microscopy remains crucial to the diagnosis of a number of disorders involving the renal glomerulus, vasculature, and tubulointerstitial compartment. The number of renal biopsies continues to grow and the indications for these biopsies continue expanding toge...
Source: Ultrastructural Pathology - December 15, 2020 Category: Pathology Authors: Howell D, Herrera GA Tags: Ultrastruct Pathol Source Type: research

Histological and Biochemical Changes in Adult Male Rat Liver after Spinal Cord Injury with Evaluation of the Role of Granulocyte-Colony Stimulating Factor.
Abstract Spinal cord injury (SCI) is a devastating disease leading to motor disability. Metabolic dysfunction is another complication of SCI. Thus, we aimed to study the effect of SCI on the histological and biochemical structure of the liver in adult male rats and to delineate the role of post-injury administration of G-CSF. Thirty adult male Sprague-Dawley rats were assigned into three groups: Group I; control (18 rats subdivided equally into three subgroups), and 12 rats underwent SCI and were divided into an SCI group II and G-SCF-treated group III. Twenty-one days post-injury, liver sections were processed fo...
Source: Ultrastructural Pathology - December 6, 2020 Category: Pathology Authors: Mohamed DA, Mohamed NM, Abdelrahaman S Tags: Ultrastruct Pathol Source Type: research

Zebra-like bodies in COVID-19: is phospholipidosis evidence of hydroxychloroquine induced acute kidney injury?
We present a similar finding in the postmortem examination of a male in his early seventies with COVID-19 infection, who received five days of HCQ treatment before stopping the medication due to cardiac and renal toxicity. PMID: 33274661 [PubMed - as supplied by publisher] (Source: Ultrastructural Pathology)
Source: Ultrastructural Pathology - December 4, 2020 Category: Pathology Authors: Obeidat M, Isaacson AL, Chen SJ, Ivanovic M, Holanda D Tags: Ultrastruct Pathol Source Type: research

Ultrastructure of the kidney filtration barrier in conditions of distant tumor growth and lithium treatment.
Abstract Toxic products generated in the process of tumor growth and as a result of treatment may cause damage to organs distant from the tumor growth, including kidneys. The aim of the work was to identify ultrastructural changes in the components of kidney filtration barrier in conditions of distant tumor growth and lithium carbonate treatment. Tumor growth was induced by the inoculation of hepatocellular carcinoma-29 cells into the right thigh muscle of CBA mice. Lithium carbonate was injected along the periphery of the tumor. Ultrastructural analysis of podocytes and endotheliocytes of glomerular capillaries i...
Source: Ultrastructural Pathology - November 30, 2020 Category: Pathology Authors: Bgatova N, Taskaeva I Tags: Ultrastruct Pathol Source Type: research

Ultrastructural effects of nerve growth factor and betamethasone on nerve regeneration after experimental nerve injury.
In conclusion, combined therapy of NGF and betamethasone may be an effective approach for the treatment of PNI. PMID: 33213258 [PubMed - as supplied by publisher] (Source: Ultrastructural Pathology)
Source: Ultrastructural Pathology - November 20, 2020 Category: Pathology Authors: Sencar L, Güven M, Şaker D, Sapmaz T, Tuli A, Polat S Tags: Ultrastruct Pathol Source Type: research

Fluorine detection in the lung tissue of a worker with interstitial pulmonary fibrosis and long-term occupational exposure to polytetrafluoroethylene and perfluorooctanoic acid.
Abstract Exposure to polytetrafluoroethylene (PTFE), a compound used in nonstick cookware coating and a variety of other applications, is known to cause acute lung injury and granulomatous pneumonitis. It is uncertain whether PTFE and compounds used in its manufacture, such as perfluorooctanoic acid (PFOA), cause chronic lung disease. Here we report a case of interstitial pulmonary fibrosis in a 71-year-old man who died following a brief illness clinically suspected to be acute respiratory distress syndrome. He had a 25-year history of occupational exposure to PTFE and PFOA. At postmortem examination, the lungs de...
Source: Ultrastructural Pathology - November 17, 2020 Category: Pathology Authors: Butnor KJ, Covington J, Taatjes DJ, DeWitt J, Von Turkovich MA Tags: Ultrastruct Pathol Source Type: research

Renal amyloidosis with emphasis on the diagnostic role of electron microscopy.
Abstract Our understanding of renal diseases with structured deposits has improved in the last two decades with the development of new diagnostic techniques that also changed the role of ultrastructural pathology in diagnostic decision-making. This review article addresses the current role of electron microscopy in the evaluation of structured deposits and discusses the impact of new developments. The diagnosis in a subset of structured deposits, amyloidosis, relies on morphologic and tinctorial characteristics at the light microscopic level. Congo red staining of tissue with demonstrable birefringence upon polari...
Source: Ultrastructural Pathology - November 9, 2020 Category: Pathology Authors: Herrera GA Tags: Ultrastruct Pathol Source Type: research

Eosinophilic globules in a classic ependymoma: evidence of a possible secretory role.
cute;pez GY Abstract A number of neoplasms of the central nervous system can demonstrate diffuse eosinophilic globules, known to be secretory products of the corresponding cell type, but they have not been a salient feature in descriptions of classic ependymoma. Here, we present a case of a posterior fossa ependymoma demonstrating glassy PAS-positive, diastase-resistant, eosinophilic globules with light microscopic and ultrastructural features resembling Reissner fiber, the secretory product of the subcommissural organ. While there has been a single published description of an ependymoma with intra- and extracellu...
Source: Ultrastructural Pathology - November 5, 2020 Category: Pathology Authors: Ahn JS, Petersen M, Friedman AH, López EM, Cummings TJ, Buckley AF, López GY Tags: Ultrastruct Pathol Source Type: research

Electron microscopic findings can support multiple etiologies of nephrotoxicity in renal tubules.
Abstract Electron microscopy (EM) has been mainly used for identifying ultrastructural abnormalities such as fusion of foot processes and immune complex deposits in glomeruli. However, electron microscopic findings in renal tubules can provide either diagnostic evidence (unique finding) or supportive evidence (additional finding) for final diagnosis. Here we present multiple situations that EM can be used for drawing conclusions of various drug-associated nephrotoxicity. Multiple cases with drug-induced nephrotoxicity are reviewed, including clinical history, EM findings, and serum creatinine (sCr) levels, prior t...
Source: Ultrastructural Pathology - November 1, 2020 Category: Pathology Authors: Zhang PL, Pancioli T, Li W, Kanaan HD Tags: Ultrastruct Pathol Source Type: research

Monoclonal glomerulopathy with features of cryoglobulinemic glomerulopathy in murine multiple myeloma model.
This study fully investigated pathologic changes in the kidneys of Vk*MYC transgenic model using light microscopy, immunofluorescence stains for kappa and lambda light chains, and electron microscopy. Compared to the kidneys of wild-type mice, the kidneys of transgenic mice showed either mesangial segmental expansion, some with associated hypercellularity, and/or thrombotic obstruction of glomerular capillaries. The glomeruli revealed stronger lambda staining than kappa light chain staining. Six out of 12 kidneys from transgenic mice showed abundant electron-dense deposits when examined ultrastructurally. The deposits were...
Source: Ultrastructural Pathology - November 1, 2020 Category: Pathology Authors: Zhang PL, Herrera GA, Liu B Tags: Ultrastruct Pathol Source Type: research

Ultrastructural investigation of synaptic alterations in the rat hippocampus after irradiation and hyperthermia.
This study aimed to investigate ultrastructural synaptic alterations in rat hippocampus after in utero exposure to irradiation (IR) and postnatal exposure to hyperthermia (HT). There were four groups in each of the time points (3rd and 6th months). IR group: Pregnant rats were exposed to radiation on the 17th gestational day. HT group: Hyperthermia was applied to the rat pups on the 10th day after their birth. IR+HT group: Both IR and HT were applied at the same time periods. Control group: No IR or HT was applied. Rat pups were sacrificed after 3 and 6 months. Thin sections from the dentate gyrus (DG) and the CA3 of ...
Source: Ultrastructural Pathology - October 30, 2020 Category: Pathology Authors: Erkanli Senturk G, Cilingir-Kaya OT, Sirvanci S, Isler C, Kemerdere R, Ulu MO, Umay C, Onat F, Ozkara C, Uzan M, Ercan F Tags: Ultrastruct Pathol Source Type: research

Ultrastructural GABA immunogold labeling in the substantia nigra pars reticulata of kindled genetic absence epilepsy rats.
Abstract Genetic Absence Epilepsy Rats from Strasbourg (GAERS) is a well-known animal model of absence epilepsy and they are resistant to electrical kindling stimulations. The present study aimed to examine possible differences in gamma-aminobutyric acid (GABA) levels and synapse counts in the substantia nigra pars reticulata anterior (SNRa) and posterior (SNRp) regions between GAERS and Wistar rats receiving kindling stimulations. Animals in the kindling group either received six stimulations in the amygdala and had grade 2 seizures or they were kindled, having grade five seizures. Rats were decapitated one hour ...
Source: Ultrastructural Pathology - October 29, 2020 Category: Pathology Authors: Sirvanci S, Akakin D, Gulcebi İdrizoglu M, Kaya OT, Karamahmutoglu T, Turgan Aşık ZN, Onat F Tags: Ultrastruct Pathol Source Type: research

ER-phagy in human atherosclerosis: an exploratory ultrastructural study.
This study has been undertaken to explore the existence of this selective control mechanism in the cells of human atherosclerotic plaques. Transmission Electron Microscopy (TEM) analysis revealed that in the plaque cells the smooth ER profiles reorganized into concentric whorls and closely packed membranes arranged in curved and parallel arrays. Circular, often ring-shaped, ER membranes studded with ribosomes and enclosed in a sequestering vesicle have been also frequently observed. This preliminary study demonstrates the existence of a distinct machinery for the specific turnover of ER membranes in human atherosclerosis a...
Source: Ultrastructural Pathology - October 29, 2020 Category: Pathology Authors: Perrotta I Tags: Ultrastruct Pathol Source Type: research

Primary ependymoma of the retropubic space in a male patient.
We report a case of primary intraabdominal ependymoma arising in the retropubic space of a male patient. An incidental intraabdominal mass was discovered in a 51-year-old man. Radiological studies revealed a 10 cm, solid and cystic tumor located in the Retzius fossa. Microscopically, the lesion was characterized by multiple cellular nodules composed of bland small cells forming true and pseudorosettes. No nuclear atypia, necrosis or increased mitotic activity was present. Neoplastic cells positive for AE1/3 and Cam5.2, and expressed patchy GFAP, and paranuclear dot-like to microvesicular EMA and D2-40, while S100, synaptop...
Source: Ultrastructural Pathology - October 29, 2020 Category: Pathology Authors: Tasar Kapakli E, Kosemehmetoglu K, Kaymaz F, Akdogan B, Ozmen M, Baydar DE Tags: Ultrastruct Pathol Source Type: research

Response to letter regarding "Talc and mesothelioma: mineral fiber analysis of 65 cases with clinicopathological correlation".
Response to letter regarding "Talc and mesothelioma: mineral fiber analysis of 65 cases with clinicopathological correlation". Ultrastruct Pathol. 2020 Jul 23;:1-2 Authors: Roggli VL, Carney JM, Sporn TA, Pavlisko EN PMID: 32700637 [PubMed - as supplied by publisher] (Source: Ultrastructural Pathology)
Source: Ultrastructural Pathology - July 22, 2020 Category: Pathology Authors: Roggli VL, Carney JM, Sporn TA, Pavlisko EN Tags: Ultrastruct Pathol Source Type: research

Improved spinal cord gray matter morphology induced by Spirulina platensis following spinal cord injury in rat models.
In this study, spirulina, known for its neuroprotective properties was used to further explore its protective effects on spinal cord gray matter ultrastructural. Twenty-four Sprague-Dawley rats were used and divided into sham group (laminectomy without SCI), control group (SCI without S. platensis), and S. platensis group (SCI + 180 mg/kg S. platensis). All animals were anesthetized via intramuscular injection. A partial crush injury was induced at the level of T12. The rats were humanely sacrificed for 28 days postinjury for ultrastructural study. There were significant mean differences with respect to pairwise comparison...
Source: Ultrastructural Pathology - July 19, 2020 Category: Pathology Authors: Abdullahi D, Ahmad Annuar A, Sanusi J Tags: Ultrastruct Pathol Source Type: research

Ultra-structural study of the indomethacin-induced apoptosis and autophagy in rat gastric parietal cells.
CONCLUSIONS: Our study indicates that IND treatment could induce gastropathy through time-dependent alterations in the autophagic and apoptotic machinery of PCs. Further studies are needed to examine the underlying molecular mechanisms. PMID: 32672114 [PubMed - as supplied by publisher] (Source: Ultrastructural Pathology)
Source: Ultrastructural Pathology - July 15, 2020 Category: Pathology Authors: Gebril SM, Ito Y, Abu-Dief EE, Hussein MRA, Elsayed HM, Mohammad AN, Abdelaal UM, Higuchi K Tags: Ultrastruct Pathol Source Type: research

Fibrillary glomerulonephritis presenting as crescentic glomerulonephritis in a young female: a case study.
We report a case of a young female who presented as RPGN but diagnosis was revised to crescentic FGN after electron microscopy and immunohistochemical staining with DNAJB9 stain. Patient remained dialysis-dependent after treatment with steroid and cyclophosphamide for 2 months and progressed to end-stage renal disease (ESRD). Crescentic FGN usually does not respond to treatment and invariably progresses to ESRD over few months. This case emphasizes the defining role of electron microscopy and special stains in diagnosing uncommon glomerular diseases. PMID: 32657244 [PubMed - as supplied by publisher] (Source: Ultrastructural Pathology)
Source: Ultrastructural Pathology - July 12, 2020 Category: Pathology Authors: Singh PP, Krishna A, Sharma A, Kumar O Tags: Ultrastruct Pathol Source Type: research

Duration-dependent effects induced by titanium dioxide nanoparticles on pancreas of adult male albino rats (histological and biochemical study).
In conclusion: the present study stated that TiO2NPs exposure for long durations had toxic effects on both exocrine and endocrine pancreas mediated by apoptotic and oxidative stress pathways. PMID: 32600082 [PubMed - as supplied by publisher] (Source: Ultrastructural Pathology)
Source: Ultrastructural Pathology - June 29, 2020 Category: Pathology Authors: Abdel Aal SM, Ahmed SM, Abdelrahman SA, Abdelrahman AA, Samy W Tags: Ultrastruct Pathol Source Type: research

Effects of combined treatment of minocycline and methylprednisolone on the expression of tumor necrosis factor alpha and interleukine-6 in experimental spinal cord injury: a light and electron microscopic study.
In this study, we aimed to evaluate effects of MP+minocycline combined treatment on cellular distribution and localization of TNF-α And IL-6 after SCI. Eighty Wistar rats were divided into three main groups as the intact control group, sham operation group, and experimental control group that received spinal cord compression injury. Following the injury, the experimental control group was subdivided into four groups as control, methylprednisolone treatment, minocycline treatment and, MP+minocycline combined treatment groups. Tissue samples were obtained from all groups at 24 hours and 72 hours after the inj...
Source: Ultrastructural Pathology - June 21, 2020 Category: Pathology Authors: Sencar L, Yilmaz DM, Tuli A, Polat S Tags: Ultrastruct Pathol Source Type: research

Suppression of type 2 diabetes mellitus-induced aortic ultrastructural alterations in rats by insulin: an association of vascular injury biomarkers.
Abstract Diabetes represents a major public health problem and an estimated 70% of people with diabetes die of cardiovascular complications. The protective effect of insulin treatment against ultrastructural damage to the tunica intima and tunica media of the aorta induced by type 2 diabetes mellitus (T2DM) has not been investigated before using transmission electron microscopy (TEM). Therefore, we induced T2DM in rats using high fat diet and streptozotocin (50 mg/kg) and administered insulin daily by i.v injection for 8 weeks to the treatment group. Whereas, the T2DM control group were left untreated for the...
Source: Ultrastructural Pathology - June 12, 2020 Category: Pathology Authors: Alzamil NM, Dawood AF, Hewett PW, Bin-Jaliah I, Assiri AS, Abdel Kader DH, Eid RA, Haidara MA, Al-Ani B Tags: Ultrastruct Pathol Source Type: research

Response to Roggli et al. (2020) "Talc and mesothelioma: mineral fiber analysis of 65 cases with clinicopathological correlation".
Response to Roggli et al. (2020) "Talc and mesothelioma: mineral fiber analysis of 65 cases with clinicopathological correlation". Ultrastruct Pathol. 2020 Jun 10;:1-2 Authors: Tran T, Egilman D, Rigler M PMID: 32520630 [PubMed - as supplied by publisher] (Source: Ultrastructural Pathology)
Source: Ultrastructural Pathology - June 9, 2020 Category: Pathology Authors: Tran T, Egilman D, Rigler M Tags: Ultrastruct Pathol Source Type: research

Metformin pretreatment suppresses alterations to the articular cartilage ultrastructure and knee joint tissue damage secondary to type 2 diabetes mellitus in rats.
Abstract Osteoarthritis (OA) secondary to diabetes affects millions of people worldwide and can lead to disability. The protective effect of metformin pretreatment against alterations to the articular cartilage ultrastructure induced by type 2 diabetes mellitus (T2DM) associated with the inhibition of oxidative stress and inflammation has not been investigated before. Therefore, we induced T2DM in rats (the model group) using high carbohydrate and fat diet and a single injection of streptozotocin (50 mg/kg body weight). The protective group of rats started metformin (200 mg/kg body weight) treatment 14 days b...
Source: Ultrastructural Pathology - May 13, 2020 Category: Pathology Authors: Dawood AF, Alzamil N, Ebrahim HA, Abdel Kader DH, Kamar SS, Haidara MA, Al-Ani B Tags: Ultrastruct Pathol Source Type: research

Ultrastructural analysis of the submandibular sialoliths: Raman spectroscopy and electron back-scatter studies.
Abstract The aim of work was the epidemiological analysis of the occurrence of sialolithiasis of the submandibular gland in adults and the evaluation of the ultrastructure of salivary stones. The study sample consisted of 44 sialoliths. Analysis of the structure and chemical composition of sialoliths was performed using a Scanning Electron Microscope and Raman Spectroscopy. Comparing our results with the literature we can say that the epidemiology of sialolithiasis has not changed significantly over the past 50 years. A wide variety of sialoliths structure was observed. In 75% (33) cases a layered structure o...
Source: Ultrastructural Pathology - April 6, 2020 Category: Pathology Authors: Tretiakow D, Skorek A, Ryl J, Wysocka J, Darowicki K Tags: Ultrastruct Pathol Source Type: research

Ex vivo interaction between blood components and hormone-dependent breast cancer cells induces alterations associated with epithelial-mesenchymal transition and thrombosis.
Abstract The prevalence of breast cancer is steadily increasing with metastasis and thromboembolic complications identified as the most common causes of death. The acquisition of an aggressive phenotype by hormone-dependent breast cancers is mediated by Transforming Growth Factor Beta 1 (TGF-β1) expression and is associated with epithelial-mesenchymal transition (EMT) and, potentially, increased propensity for thrombosis. We investigated this phenomenon by assessing the effect of platelet-rich plasma (PRP) and whole blood (WB) on parameters of EMT and hypercoagulation in vitro. MCF-7 breast cancer cells were ...
Source: Ultrastructural Pathology - April 5, 2020 Category: Pathology Authors: Augustine TN, Pather K, Mak D, Klonaros D, Xulu K, Dix-Peek T, Duarte R, van der Spuy WJ Tags: Ultrastruct Pathol Source Type: research

Sealing effects of different Chinese herbal medicines on dentinal tubules: a scanning electron microscopic observation.
Abstract Background and objectives: To evaluate the sealing effects of different Chinese herbal medicines on dentinal tubules, and to provide a reference for the clinical treatment of dentin hypersensitivity.Methods: Forty dentin slices prepared by freshly extracted bovine mandibular central incisors were randomly assigned to procyanidins, tannic acid, gallic acid, naringin, epigallocatechin gallate (EGCG), glycyrrhizic acid, paeonol, and blank groups. Dentin slices in each Chinese herbal medicine group were treated three times a day, each for 5 min, and then immersed in a remineralization solution for the rest of...
Source: Ultrastructural Pathology - April 1, 2020 Category: Pathology Authors: Li Y, Chen J, Duan Y, Zhou Z Tags: Ultrastruct Pathol Source Type: research

MicroRNA-195 vector influence on the development of gradually induced hepatocellular carcinoma in murine model.
This study was designed to induce HCC, in an experimental model, with the prospect to study the molecular pathophysiologic changes accompanying the development of HCC and the effect of miRNA-195 vector on the process of hepatocarcinogenesis.Methodology: This study incorporated three groups of male albino mice; one control group and two other groups injected intraperitoneal with diethylnitrosamine (DEN) weekly for 12 weeks for the gradual induction of HCC. The third group was injected intra-hepatic with miR-195 vector 1 month after DEN injection. At the 8th and 12th weeks post-DEN treatment, the tumor-associated b...
Source: Ultrastructural Pathology - March 25, 2020 Category: Pathology Authors: El-Ahwany E, Mansy SS, Zoheiry M, Mourad L, Mahmoud S, Abu-Taleb H, Hassanien M, Hassan M Tags: Ultrastruct Pathol Source Type: research

Talc and mesothelioma: mineral fiber analysis of 65 cases with clinicopathological correlation.
Abstract Malignant mesothelioma is strongly associated with prior asbestos exposure. Recently there has been interest in the role of talc exposure in the pathogenesis of mesothelioma. We have analyzed lung tissue samples from a large series of malignant mesothelioma patients. Asbestos bodies were counted by light microscopy and mineral fiber concentrations for fibers 5 µm or greater in length were determined by scanning electron microscopy equipped with an energy dispersive spectrometer. The values were compared with 20 previously published controls. Among 609 patients with mesothelioma, talc fibers wer...
Source: Ultrastructural Pathology - March 17, 2020 Category: Pathology Authors: Roggli VL, Carney JM, Sporn TA, Pavlisko EN Tags: Ultrastruct Pathol Source Type: research

Comparison of ultrastructural changes and the anticarcinogenic effects of thymol and carvacrol on ovarian cancer cells: which is more effective?
Abstract Ovarian cancer is the seventh most common cancer worldwide in women. Many anticancer drugs are currently used clinically have been isolated from plant species or are based on such substances. Thymol (5-methyl-2-isopropylphenol) and carvacrol are oxygenated aromatic compounds from the monoterpene group. They are the main constituents of thyme essential oil and show antiproliferative, antioxidant, and antiseptic properties. The aim of this study is to compare the antiproliferative and apoptotic effects of thymol and carvacrol on SKOV-3 ovarian cancer cell line. The cancer cells were treated with different c...
Source: Ultrastructural Pathology - March 16, 2020 Category: Pathology Authors: Elbe H, Yigitturk G, Cavusoglu T, Baygar T, Ozgul Onal M, Ozturk F Tags: Ultrastruct Pathol Source Type: research

Podocyte infolding glomerulopathy with undifferentiated connective tissue disease: a case report.
Abstract Podocyte infolding glomerulopathy (PIG) is a special type of glomerular disease that has been proposed in recent years and has attracted considerable attention. PIG is characterized by the formation of microspheres and microtubules in thickened glomerular basement membrane (GBM) on electron microscopy (EM), which is recognized as podocyte cytoplasmic infolding to the GBM. However, to date, only a few cases of PIG have been reported. Herein, we report a case of a 33-year-old female with PIG with undifferentiated connective tissue disease (UCTD) in China and review the literature. PMID: 32172632 [PubMe...
Source: Ultrastructural Pathology - March 13, 2020 Category: Pathology Authors: Shi J, Zheng R, Gao H, Zhao Z, Wu H, Zhang Z Tags: Ultrastruct Pathol Source Type: research

Sinonasal amelanotic melanoma with neuroendocrine differentiation: a diagnostic conundrum.
We report a rare case of a 63-year-old female who developed sinonasal amelanotic melanoma with immunohistochemical expression of neuroendocrine markers, presenting a diagnostic dilemma. Ultrastructural evidence of melanosomes and neurosecretory granules aided in arriving at the diagnosis. Aberrant immunoexpression of neuroendocrine markers, particularly in an amelanotic melanoma, has critical diagnostic implications, as various malignancies with undifferentiated histomorphology occur at this site, many of which stain positively with neuroendocrine markers. We discuss the differential diagnoses and recommend a high index of...
Source: Ultrastructural Pathology - March 11, 2020 Category: Pathology Authors: Kaur K, Kakkar A, Rastogi S, Sharma MC Tags: Ultrastruct Pathol Source Type: research

An ultrastructural pathologist's views on fibroblasts, modified smooth muscle cells, wound healing, stenosing arteriopathies, Kawasaki disease, Dupuytren's contracture, and the stroma of carcinomas.
Abstract It wasn't until 1960 that the dense bodies of the peripheral actin arrays of fibroblasts were finally visualized, i.e., stress fibers (SFs). Mistakenly assumed that its SFs turned the fibroblast into a unique cell situated somewhere in a continuum between it and a smooth muscle cell (SMC), it was descriptively named a "myofibroblast" (MF). Automatically, spindle cells with SFs and/or smooth muscle actin by SMA IHC-staining, became MFs, although endothelial cells, pericytes, modified SMCs (mSMC), and myoepithelial cells all contain SFs. An invisible "intermediate" cell was hypothesized ...
Source: Ultrastructural Pathology - March 9, 2020 Category: Pathology Authors: Orenstein JM Tags: Ultrastruct Pathol Source Type: research

Ultrastructural evidence for presen сe of gap junctions in rare case of pleomorphic xanthoastrocytoma.
Ultrastructural evidence for presenсe of gap junctions in rare case of pleomorphic xanthoastrocytoma. Ultrastruct Pathol. 2020 Mar 09;:1-10 Authors: Kirichenko EY, Salah M M S, Goncharova ZA, Nikitin AG, Filippova SY, Todorov SS, Akimenko MA, Logvinov AK Abstract The phenomenon of unstable expression of gap junction's proteins connexins remains a "visiting card" of astrocytic tumors with various degrees of malignancy. At the same time, it stays unclear what is detected by the positive expression of connexins in astrocytic tumors: gap junctions, hemi-channels, or connexin proteins in cytosol...
Source: Ultrastructural Pathology - March 8, 2020 Category: Pathology Authors: Kirichenko EY, Salah M M S, Goncharova ZA, Nikitin AG, Filippova SY, Todorov SS, Akimenko MA, Logvinov AK Tags: Ultrastruct Pathol Source Type: research

Ultrastructure of CIC-DUX4 sarcoma: the first pathological report.
We examined two cytogenetically confirmed CDS cases. We found that, compared to typical ES, CDS presented heterogeneity: in cell density, from tightly packed to loosely unconnected areas; in cell shape, from polygonal to pleomorphic with small processes; and in nuclear shape including round, oval, polygonal, elongated, invaginated, or wrinkled formations. However, abundant glycogen in the cytoplasm and rare cell adhesion apparatus between cells are major similarities between CDS and typical ES. Neuroendocrine granules, which are seen in rare ES cases, could not be identified in these two CDS cases. Although cytogenetic dif...
Source: Ultrastructural Pathology - March 8, 2020 Category: Pathology Authors: Tamada H, Kobayashi M, Sano K, Uehara T, Matsumoto Y, Tateishi A, Ohya M, Kito M, Aoki K, Kanno H Tags: Ultrastruct Pathol Source Type: research

Induction of hepatic portal fibrosis, mitochondria damage, and extracellular vesicle formation in Sprague-Dawley rats exposed to copper, manganese, and mercury, alone and in combination.
In conclusion, Cu, Mn, and Hg alone and as part of a mixture cause cellular damage, inflammation, and fibrosis increasing the risk of associated diseases. PMID: 32091299 [PubMed - as supplied by publisher] (Source: Ultrastructural Pathology)
Source: Ultrastructural Pathology - February 23, 2020 Category: Pathology Authors: Dhanraj P, Venter C, Bester MJ, Oberholzer HM Tags: Ultrastruct Pathol Source Type: research

Retinal and choroidal capillaries contribution to age-related macular degeneration (AMD) phenotypes in murine models of the disease.
Abstract Mouse models of age-related macular degeneration (AMD) such as Ccl2-/- and Ccl2-/-/Cx3cr1-/- have not yet been fully characterized ultrastructurally. Although we have previously shown extranuclear DNA (enDNA) leakage into the cytoplasm and damaged mitochondria in the retinal pigment epithelium (RPE) of these AMD mouse models, little is known about the state of their vascular capillaries of the retina and choroid. Our ultrastructural survey shows that the aberrations were not restricted to the RPE cells, but also extended to the vasculature of the retina and choroid. Their endothelial aberrations included ...
Source: Ultrastructural Pathology - February 20, 2020 Category: Pathology Authors: Ahsan B, Aldwaikat A, Aboud O, Ramadan A, Abu-Asab MS Tags: Ultrastruct Pathol Source Type: research

Histological study of the effect of granulocyte colony-stimulating factor on experimentally induced corneal burn in adult male albino rats.
Abstract Chemical injuries to the eye represent one of the true ophthalmic emergencies that require immediate and intensive intervention to minimize severe complications and visual loss. Granulocyte colony-stimulating factor (G-CSF) is a potent hematopoietic cytokine that influences the proliferation, survival, maturation, and the functional activation of granulocytes. The present work was performed to evaluate the histological effect of G-CSF in treating rat corneal alkali burn model. Thirty adult male albino rats were divided equally into three main groups: Group I was served as a control group, and in Group II ...
Source: Ultrastructural Pathology - February 20, 2020 Category: Pathology Authors: Elabd SS, Abo-Elnasr SE, Soliman GM, Sarhaan NI, Tawfik SM Tags: Ultrastruct Pathol Source Type: research