The potential neuroprotective role of mesenchymal stem cell-derived exosomes in cerebellar cortex lipopolysaccharide-induced neuroinflammation in rats: a histological and immunohistochemical study.
This study aimed to evaluate the potential protective role of bone marrow mesenchymal stem cell-derived exosomes (BMSC-EXs) in cerebellar cortex LPS-induced neuroinflammation in rats. Twenty-seven adult male rats were divided into three groups: Group I: control rats; Group II: LPS-treated rats; Group III: LPS/BMSC-EXs-treated rats. Cerebellar specimens were taken and processed for histological and immunohistochemical analysis. Morphometrical studies and statistical analysis were done. Groups II showed neuronal degeneration and apoptosis. The mean number of Purkinje cells was significantly (P
Source: Ultrastructural Pathology - February 10, 2020 Category: Pathology Authors: El-Mahalaway AM, El-Azab NE Tags: Ultrastruct Pathol Source Type: research

Intrahepatocellular crystal storing mimicking a clinical liver disease during monoclonal gammopathy: report of a case and review of the literature.
We present an unusual case of liver involvement in monoclonal gammopathy with generalized crystal-storing histiocytosis (G-CSH).A bone marrow storage disease was diagnosed in a 79-year-old man with monoclonal gammopathy of uncertain significance (MGUS). The patient presented with pleural effusion, an osteolytic lesion of the humerus, and an increase of aspartate transaminase and cholestatic markers that raised the clinical suspect of liver disease. A second bone marrow biopsy confirmed the diagnosis of MGUS with a histiocytic component suggestive for G-CSH.Liver biopsy showed an unremarkable histology, no significant infla...
Source: Ultrastructural Pathology - February 10, 2020 Category: Pathology Authors: Riefolo M, Malvi D, Bertuzzi C, Sabattini E, Valente S, Pasquinelli G, D'Errico A, Vasuri F Tags: Ultrastruct Pathol Source Type: research

Early histological and ultrastructural changes in expanded murine scalp.
Abstract Tissue expansion has been widely used for plastic, reconstructive, and esthetic surgeries. A mouse scalp expansion model can effectively mimic the characteristics of human skin expansion. However, a detailed study of the histological features and ultrastructural characteristics of expanded scalp is lacking, especially early ultrastructural changes. Here, a mouse scalp expansion model was established and the expanded scalp samples were obtained on day 2 (group I) and 4 (group II) post final injection. Histological analysis revealed epidermal thickening, dermal thinning, subcutaneous fat thinning, and capsu...
Source: Ultrastructural Pathology - January 28, 2020 Category: Pathology Authors: Yu Z, Liu S, Cui J, Song Y, Wang T, Song B, Peng P, Ma X Tags: Ultrastruct Pathol Source Type: research

Renal ultrastructural alterations induced by various preparations of mefenamic acid.
Abstract Mefenamic acid (MFA) treatment is associated with a number of cellular effects that potentiate the incidence of renal toxicity. The aim of this study is to investigate the potential ultrastructural alterations induced by various preparations of MFA (free MFA, MFA-Tween 80 liposomes, and MFA-DDC liposomes) on the renal tissues. Sprague-Dawley rats were subjected to a daily dose of MFA preparations for 28 days. Renal biopsies from all groups of rats under study were processed for transmission electron microscopic examination. The findings revealed that MFA preparations induced various ultrastructural altera...
Source: Ultrastructural Pathology - January 22, 2020 Category: Pathology Authors: Jarrar QB, Hakim MN, Zakaria ZA, Cheema MS, Moshawih S Tags: Ultrastruct Pathol Source Type: research

Tramadol administration induced hippocampal cells apoptosis, astrogliosis, and microgliosis in juvenile and adult male mice, histological and immunohistochemical study.
Abstract Tramadol is a common analgesic, frequently used for relieving moderate or severe pain and widely used to delay ejaculation. However, repeated large doses have several adverse effects, especially on the brain tissue. So, this study was designed to assess the potentially deleterious effects of chronic administration of tramadol on principal fields of the hippocampus in adult and juvenile male albino mice. Thirty swiss male albino mice were divided equally into three groups: Group Ia (control adult) 3 months old, Group Ib (control juvenile) 3-week postnatal mice, Group II (tramadol treated adult mice) and Gr...
Source: Ultrastructural Pathology - January 10, 2020 Category: Pathology Authors: Hussein OA, Abdel Mola AF, Rateb A Tags: Ultrastruct Pathol Source Type: research

Tissue burden evaluation of elongated mineral particles in two individuals with mesothelioma and whose work history included manufacturing tile.
Abstract Two cases with diagnosis of mesothelioma were referred to our laboratories with a request for tissue burden analysis in order to determine the presence of ferruginous bodies and uncoated elongated mineral particles in tissue samples. The individuals shared in common a past background of working in tile manufacturing facilities where industrial talc was used in the production of the products. Both were found to have ferruginous bodies in their lung tissues as well as elongated talc fibers/ribbons and elevated numbers of noncommercial amphiboles in their tissues. To our knowledge, this is the first report o...
Source: Ultrastructural Pathology - January 9, 2020 Category: Pathology Authors: Dodson RF, Poye LW Tags: Ultrastruct Pathol Source Type: research

Foam cell origination from degenerated vascular smooth muscle cells in atherosclerosis: An ultrastructural study on hyperlipidemic rabbits.
Abstract To clarify foam cell origination in atherosclerosis, a series of morphologic and ultrastructural alterations of vascular smooth muscle cells (VSMCs) and foam cells were studied by light and electron microscopy in atherosclerotic aortas from hyperlipidemic rabbits induced for 5 weeks. The study exhibited that VSMCs were severely degenerated and damaged, including irregular shapes, expanded mitochondria, aplenty lipid droplets, and disarranged myofilaments in cytoplasm in media adjacent to atheromatic bottoms. Most lipid laden cells shared interphase structures of VSMCs and foam cells, and some dissolved sp...
Source: Ultrastructural Pathology - January 7, 2020 Category: Pathology Authors: Ru YX, Shang HC, Dong SX, Zhao SX, Liang HY, Zhu CJ Tags: Ultrastruct Pathol Source Type: research

Investigation of the preventive effects of dehydroepiandrosterone (DHEA) and Caffeic acid phenethyl ester (CAPE) on cisplatin-induced ovarian damage in rats.
tanağası AO Abstract To investigate whether Dehydroepiandrosterone (DHEA) and Caffeic acid phenethyl ester (CAPE) had any preventive effect against the ovarian damage caused by cisplatin (CP) (cis-diamminedichloroplatinum) in rats. On the first day ovaries were removed, Anti-Müllerian hormone (AMH) was measured (Group1, n:6), in the other groups 7.5 mg/kg cisplatin was administered intraperitoneally. In Group 2 (n = 6), 0.1 ml saline, in Group 3 (n = 5), 20 umol/kg CAPE, in Group 4 (n = 7), DHEA 6 mg/kg were administered every day. On the 10th day, ovaries were removed, AMH was...
Source: Ultrastructural Pathology - January 7, 2020 Category: Pathology Authors: Anğın AD, Gün I, Sakin Ö, Çıkman MS, Şimşek EE, Karakuş R, Başak K, Kaptanağası AO Tags: Ultrastruct Pathol Source Type: research

Effects of a high-fat diet on intracellular calcium (Ca2+) handling and cardiac remodeling in Wistar rats without hyperlipidemia.
In this study, rats were randomly divided into two groups: the standard diet (N = 5) and high-fat diet (N = 5) groups. To evaluate the effects of a high-fat diet on cardiac remodeling, we investigated the myocardium obtained from male Wistar rats fed a high-fat diet or standard diet for ten weeks via scanning electron microscopy, polarization microscopy, and RT-PCR. We found that compared with the standard diet cohort, the high-fat diet cohort exhibited increased levels of SERCA2a and SERCA2b mRNA and a decreased level of PLB mRNA (P
Source: Ultrastructural Pathology - January 5, 2020 Category: Pathology Authors: He Y, Shi M, Wu J, Sun Z, Guo J, Liu Y, Han D Tags: Ultrastruct Pathol Source Type: research

Use of electron microscopy when screening liver biopsies from neonates and infants: experience from a single tertiary children's hospital (1991-2017).
Abstract Background: Although the role of electron microscopy is diminishing in several areas of adult pathology, it remains an essential tool for the study of pediatric liver biopsies.Methods: Clinical charts, histologic slides and EM materials of native liver biopsies from patients
Source: Ultrastructural Pathology - January 3, 2020 Category: Pathology Authors: Warren M, Shimura M, Wartchow EP, Yano S Tags: Ultrastruct Pathol Source Type: research

Histological and ultrastructural study of AflatoxinB1 induced neurotoxicity in Sciatic nerve of adult male Albino rats.
Abstract Aflatoxins are mycotoxins produced by Aspergillus spp. which is a common contaminant of food items such as corn, spices, rice, nuts, and flour. Aflatoxin contamination of foods is a worldwide problem. Chronic aflatoxin exposure is found to be associated with Sciatic nerve damage. In vivo study was carried out to evaluate the toxic effect of aflatoxin B1 (AFB1) on the Sciatic nerve. Twenty-one adult male rats were included and divided equally into 3 groups (7 rats each): Group I (control group), group II (olive oil group) and group III: (AflatoxinB1 group). The rats received AFB1 (250 μg/kg B.W./day) or...
Source: Ultrastructural Pathology - January 1, 2020 Category: Pathology Authors: Makhlouf MMM Tags: Ultrastruct Pathol Source Type: research

Early ultra- and microstructural alterations in rat pancreas in alloxan-induced diabetes mellitus.
Abstract An adequate experimental model is important to understand pathophysiological processes ongoing in the pancreas with diabetes mellitus. Our study was aimed to describe early ultra- and microstructural changes in the rat pancreas in 12-48 h after alloxan administration in a dose of 180 mg/kg. A histopathological examination of the endocrine pancreas revealed the loss of borders between endocrine cells, granular dystrophy and degranulation, sings of necrosis in central cells of the Langerhans islets and apoptosis of their peripheral ones manifested as DNA fragmentation and an increased expression of apoptosi...
Source: Ultrastructural Pathology - December 31, 2019 Category: Pathology Authors: Titova AA, Mavlikeev MO, Kaligin MS, Suleymanova DM, Chekmaryeva IA, Kiyasov AP, Deev RV Tags: Ultrastruct Pathol Source Type: research

Comments on the manuscript of Dr. J M Orenstein.
PMID: 31875456 [PubMed - as supplied by publisher] (Source: Ultrastructural Pathology)
Source: Ultrastructural Pathology - December 25, 2019 Category: Pathology Authors: Gabbiani G Tags: Ultrastruct Pathol Source Type: research

Response to Letter Regarding 'Dimensions of Elongated Mineral Particles: A Study of More Than 570 Fibers From More Than 90 Cases with Implications for Pathogenicity and Classification as Asbestiform vs. Cleavage Fragments'.
PMID: 31852394 [PubMed - in process] (Source: Ultrastructural Pathology)
Source: Ultrastructural Pathology - December 21, 2019 Category: Pathology Authors: Roggli VL, Green CL Tags: Ultrastruct Pathol Source Type: research

The value of ultrastructural evaluation in medical renal diseases.
PMID: 31852395 [PubMed - in process] (Source: Ultrastructural Pathology)
Source: Ultrastructural Pathology - December 21, 2019 Category: Pathology Authors: Herrera GA Tags: Ultrastruct Pathol Source Type: research

Lithium effects on vesicular trafficking in hepatocellular carcinoma cells.
Abstract Hepatocellular carcinoma (HCC) is one of the most commonly malignant tumors worldwide, characterized by the presence of many heterogeneous molecular cell events that contribute to tumor growth and progression. Endocytic processes are intimately involved in various pathological conditions, including cancer, since they interface with various cellular signaling programs. The ability of lithium to induce cell death and autophagy and affect cell proliferation and intracellular signaling has been shown in various experimental tumor models. The aim of this study was to evaluate the effects of lithium on vesicula...
Source: Ultrastructural Pathology - December 11, 2019 Category: Pathology Authors: Taskaeva I, Bgatova N, Gogaeva I Tags: Ultrastruct Pathol Source Type: research

Complementary value of electron microscopy and immunohistochemistry in the diagnosis of non-small cell lung cancer: A potential role for electron microscopy in the era of targeted therapy.
ute;, Hernández S, Lloveras B, Lloreta Trull J Abstract With the identification of therapeutic targets for lung adenocarcinoma, it has become mandatory to distinguish it from other entities. Some cases remain classified as non-small cell lung carcinoma, not otherwise specified (NSCLC-NOS) with immunohistochemistry. Electron microscopy (EM) can be useful, allowing the identification of glandular differentiation. The aim of this study was to determine the complementary value of immunohistochemistry and EM.Forty-eight NSCLC-NOS cases were selected (PSMAR-Biobank, Barcelona, Spain). Immunohistochemistry (TTF-1,...
Source: Ultrastructural Pathology - December 6, 2019 Category: Pathology Authors: Albero-González R, Munné-Collado J, Pijuan L, Simón M, Gimeno-Beltrán J, Mojal S, Salido M, Clavé S, Juanpere N, Dalmases A, Comerma L, Vázquez I, Sánchez-Font A, Taus Á, Hernández S, Lloveras B, Lloreta Trull J Tags: Ultrastruct Pathol Source Type: research

Is the hepatocyte ultrastructural zonal heterogeneity changed by overnight (16 h) fasting? Morphometric study.
Conclusion: The organelles appeared normal with no signs of degeneration and the zonal differences were maintained after fasting. The change in hepatocyte ultrastructure after fasting may be related to autophagy. PMID: 31791174 [PubMed - as supplied by publisher] (Source: Ultrastructural Pathology)
Source: Ultrastructural Pathology - December 2, 2019 Category: Pathology Authors: Elsayed HRH, El Nashar EM, Abd-Elmonem MM Tags: Ultrastruct Pathol Source Type: research

Neuroprotective potential of Spirulina platensis on lesioned spinal cord corticospinal tract under experimental conditions in rat models.
In this study, the neuroprotective potential of Spirulina platensis was investigated on ultrastructural and functional recovery of the spinal cord following surgical-induced injury. Twenty-four Sprague-Dawley rats were divided into three groups; sham group, control (trauma) group, and experimental (S. platensis) group (180 mg/kg) of eight rats each. For each group, the rats were then subdivided into two groups to allow measurement at two different timepoints (day 14 and 28) for the microscopic analysis. Rats in the control and experimental S. platensis groups were subjected to partial crush injury at the level of T12 with ...
Source: Ultrastructural Pathology - November 28, 2019 Category: Pathology Authors: Abdullahi D, Ahmad Annuar A, Sanusi J Tags: Ultrastruct Pathol Source Type: research

Myoepithelioma of bone: ultrastructural, immunohistochemical and molecular study of three cases.
Abstract Primary intraosseous myoepithelial tumors are rare neoplasms with only a handful of cases described in the medical literature. To date, intraosseous variant of benign myoepithelioma, due to its rarity, has not been studied ultrastructurally, and only one case of a malignant intraosseous myoepithelioma has been described. Three cases were retrieved from the files at the Massachusetts General Hospital (MGH). A diagnosis of benign myoepithelioma was made in case 1 and malignant epithelioma in cases 2 and 3. Ultrastructurally, intermediate filaments (without dense bodies) were found in each case with an abund...
Source: Ultrastructural Pathology - November 25, 2019 Category: Pathology Authors: Kurzawa P, Selig MK, Kraiński P, Dopierała M, Nielsen GP Tags: Ultrastruct Pathol Source Type: research

Comments on "Dimensions of elongated mineral particles with implications for pathogenicity and classification as asbestiform versus cleavage fragments".
Comments on "Dimensions of elongated mineral particles with implications for pathogenicity and classification as asbestiform versus cleavage fragments". Ultrastruct Pathol. 2019 Nov 23;:1-4 Authors: Finkelstein MM Abstract Roggli and Green have reported their study of the dimensions of fibers extracted from the tissues of cases examined in their laboratory, and have drawn conclusions about the pathogenicity of Elongated Mineral Particles and their classification as asbestiform versus cleavage fragments. There are aspects of their methodology and discussion that would benefit from examination...
Source: Ultrastructural Pathology - November 23, 2019 Category: Pathology Authors: Finkelstein MM Tags: Ultrastruct Pathol Source Type: research

Multiple calcifying fibrous pseudotumors of the pleura: ultrastructural analysis provides insight on mechanism of dissemination.
We present a 59-year-old male with multiple CFP of the pleura. Reactive-appearing adhesions spanning the pleural surfaces were present, and by electron microscopy, were involved by tumor. We suggest this is the likely mode of dissemination across the pleural surfaces. PMID: 31746679 [PubMed - in process] (Source: Ultrastructural Pathology)
Source: Ultrastructural Pathology - November 22, 2019 Category: Pathology Authors: Massoth LR, Selig MK, Little BP, Chebib I, Kradin RL Tags: Ultrastruct Pathol Source Type: research

Magnesium/silicon atomic weight percent ratio standards for the tissue identification of talc by scanning electron microscopy and energy dispersive X-ray analysis.
We examined 401 Mg-Si particles with SEM/EDS across the two samples, using two different SEM microscopes. Overall, we found a mean Mg/Si atomic weight % ratio of 0.645, with minimal differences between study subsets. The standard deviation was 0.025; (± 1σ = 0.620-0.670). The currently used ± 5% diagnostic range (Mg/Si 0.617-0.681) is thus reasonably close to this study's ± 1σ range, and well within a ± 2 σ confidence interval span (Mg/Si 0.595-0.695). The ± 5% range is thus an appropriately conservative standard whose continued use seems justified. P...
Source: Ultrastructural Pathology - November 16, 2019 Category: Pathology Authors: McDonald SA, Fan Y, Rogers RA, Godleski JJ Tags: Ultrastruct Pathol Source Type: research

Brain microvascular pathology in Susac syndrome: an electron microscopic study of five cases.
Abstract Susac syndrome is a rare, immune-mediated disease characterized by encephalopathy, branch retinal artery occlusion, and hearing loss. Herein, we describe the electron microscopic findings of three brain biopsies and two brain autopsies performed on five patients whose working clinical diagnosis was Susac syndrome. In all five cases, the key findings were basement membrane thickening and collagen deposition in the perivascular space involving small vessels and leading to thickening of vessel walls, narrowing, and vascular occlusion. These findings indicate that Susac syndrome is a microvascular disease. Mo...
Source: Ultrastructural Pathology - November 16, 2019 Category: Pathology Authors: Agamanolis DP, Prayson RA, Asdaghi N, Gultekin SH, Bigley K, Rennebohm RM Tags: Ultrastruct Pathol Source Type: research

A rare case of intracranial extra-axial ependymoma.
Abstract Intracranial extra-axial ependymomas (IEAEs) are extremely uncommon tumors and they could have a wide spectrum of clinical and radiological features. Here we report morphological features of an extra-axial ependymoma (radiology, histology and ultrastructural details) which mimicked the presentation of meningioma. PMID: 31684796 [PubMed - as supplied by publisher] (Source: Ultrastructural Pathology)
Source: Ultrastructural Pathology - November 4, 2019 Category: Pathology Authors: Fabbri VP, Papa V, Fioravanti A, Messia M, Tallini G, Asioli S, Cenacchi G Tags: Ultrastruct Pathol Source Type: research

Pseudolinear C4d deposits in a hereditary glomerulopathy caused by a rare NC1 collagen-4-alpha-5 missense mutation: a "new disease entity"?
Pseudolinear C4d deposits in a hereditary glomerulopathy caused by a rare NC1 collagen-4-alpha-5 missense mutation: a "new disease entity"? Ultrastruct Pathol. 2019 Nov 04;:1-7 Authors: Roy S, Nalwa A, Keith J, Weck K, Singh H, Nickeleit V Abstract C4d positive glomerulopathies with pseudolinear capillary wall deposits caused by basement membrane (GBM) remodeling have sporadically been reported in renal transplants. Here we describe the case of a hypertensive 60 year-old male with a 5 month history of nephrotic range proteinuria in the setting of normal serum creatinine, complement and ...
Source: Ultrastructural Pathology - November 4, 2019 Category: Pathology Authors: Roy S, Nalwa A, Keith J, Weck K, Singh H, Nickeleit V Tags: Ultrastruct Pathol Source Type: research

Localization of transmembrane mucin MUC1 on the apical surface of oral mucosal cells.
AM Abstract The purpose of the present study was to demonstrate the localization of transmembrane mucin MUC1 on the outer layer of oral mucosal cells and the involvement of apical cell surface microplicae (MPL) in bioadhesion of MUC1. Tissue samples of six healthy subjects were obtained. First, the presence of MUC1 was examined with an immunohistochemical method using a monoclonal MUC1 antigen called HFMG1. Second, the localization of MUC1 was examined with immuno-scanning electron microscopy. Immunohistochemically, high intense staining for MUC1 (antigen HFMG1) was detected in the epithelial superficial layers. ...
Source: Ultrastructural Pathology - November 2, 2019 Category: Pathology Authors: Sirviö E, Mikkonen JJW, Koistinen AP, Miinalainen I, Kullaa AM Tags: Ultrastruct Pathol Source Type: research

Testicular protective effects of ellagic acid on monosodium glutamate-induced testicular structural alterations in male rats.
This study aims to assess the ameliorative effect of EA on oxidative stress and testicular damage induced by MG. In total, 48 male rats were included in this study and separated into six groups: control, EA (20 mg/kg), MG (low dose) (17.5 mg/kg), MG (high dose) (60 mg/kg), MG (low dose) combined with EA, and MG (high dose) combined with EA. Testicular antioxidant biomarkers [superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione reductase (GRx), catalase (CAT), myeloperoxidase (MPO), and xanthine oxidase (XO)] were examined. Testes were examined and scored for histological variation as an indicator of testic...
Source: Ultrastructural Pathology - October 28, 2019 Category: Pathology Authors: Hamza RZ, Al-Baqami NM Tags: Ultrastruct Pathol Source Type: research

Suppression of acetaminophen-induced hepatocyte ultrastructural alterations in rats using a combination of resveratrol and quercetin.
Abstract Ingestion of a toxic dose of the analgesic drug, acetaminophen (also called paracetamol or APAP), is among the most common causes of acute liver injury in humans. We tested the hypothesis that the combined polyphenolic compounds, resveratrol (RES) and quercetin (QUR), can substantially protect against hepatocyte ultrastructural damage induced by a toxic dose of APAP in a rat model of APAP-induced acute liver injury. The model group of rats received a single dose of APAP (2 g/kg), whereas the protective group of rats was pretreated for 7 days with combined doses of RES (30 mg/kg) and QUR (50 mg/kg) before ...
Source: Ultrastructural Pathology - October 19, 2019 Category: Pathology Authors: Al Humayed S, Al-Ani B, El Karib AO, Shatoor AS, Eid RA, Aziz S, Wani JI, Haidara MA Tags: Ultrastruct Pathol Source Type: research

Vitamin E protects against monosodium glutamate-induced acute liver injury and hepatocyte ultrastructural alterations in rats.
Abstract Food additives such as nitrates and nitrites, and monosodium glutamate (MSG) used in the food industry increase the risk of certain cancers and inflict damage to vital organs. We sought to determine whether the antioxidant vitamin E can protect against liver injuries induced by a toxic dose of MSG in a rat model of MSG-induced acute liver injury. The model group of rats received a daily dose of MSG (4 gm/kg) for 7 days, whereas the protective groups were either received a 100 mg/kg vitamin E plus MSG or 300 mg/kg vitamin E plus MSG for 7 days. Rats were then sacrificed at day 8. Transmissio...
Source: Ultrastructural Pathology - October 10, 2019 Category: Pathology Authors: Eid RA, Al-Shraim M, Zaki MS, Kamar SS, Abdel Latif NS, Negm S, Al-Ani B, Haidara MA Tags: Ultrastruct Pathol Source Type: research

Differentiation and plasticity of human vascular wall mesenchymal stem cells, dermal fibroblasts and myofibroblasts: a critical comparison including ultrastructural evaluation of osteogenic potential.
In this study, we compared the immunophenotype and the differentiation potential of human vascular wall mesenchymal stem cells (hVW-MSCs) with those of human dermal fibroblasts and myofibroblasts. Cell morphology and surface markers were evaluated by immunofluorescence and flow cytometry; functional assays for immunomodulation, angiogenesis, adipogenesis and osteogenesis were performed, together with the mRNA analysis of the critical differentiation genes. hVW-MSCs, dermal fibroblasts and myofibroblasts were all negative to CD34, whereas the expression of CD44 stemness marker was more intense in hVW-MSCs. As expected, hVW-...
Source: Ultrastructural Pathology - October 7, 2019 Category: Pathology Authors: Pasanisi E, Ciavarella C, Valente S, Ricci F, Pasquinelli G Tags: Ultrastruct Pathol Source Type: research

Severe hepatotoxicity induced by efavirenz in a treatment-na ïve, low body mass index HIV-infected, female patient with no hepatitis and other virus co-infections.
Severe hepatotoxicity induced by efavirenz in a treatment-naïve, low body mass index HIV-infected, female patient with no hepatitis and other virus co-infections. Ultrastruct Pathol. 2019 Oct 02;:1-4 Authors: Dragovic G, Nikolic K, Dimitrijevic B, Jevtovic D, Salemovic D, Tomanovic N, Boricic I Abstract Combined antiretroviral therapy (cART) consisting of two nucleoside reverse transcriptase inhibitors (NRTIs) and one non-nucleoside reverse transcriptase inhibitor (NNRTI), such as efavirenz, is still the first-line treatment in resource-limited settings. However, efavirenz has shown strong promin...
Source: Ultrastructural Pathology - October 2, 2019 Category: Pathology Authors: Dragovic G, Nikolic K, Dimitrijevic B, Jevtovic D, Salemovic D, Tomanovic N, Boricic I Tags: Ultrastruct Pathol Source Type: research

Clinical & ultrastructural evaluation of the effect of fractional CO2 laser on facial melasma.
Clinical & ultrastructural evaluation of the effect of fractional CO2 laser on facial melasma. Ultrastruct Pathol. 2019 Oct 01;:1-10 Authors: El-Sinbawy ZG, Abdelnabi NM, Sarhan NE, Elgarhy LH Abstract Melasma represents the most obvious and disfiguring change of the face leading to psychological problems especially in females. Ablative lasers have also been used by many professionals to treat melasma, although there is few scientific data supporting this indication. The exact mechanism of action of ablative lasers in melasma is not yet clear enough. We aimed to evaluate the ultrastructural effect...
Source: Ultrastructural Pathology - October 1, 2019 Category: Pathology Authors: El-Sinbawy ZG, Abdelnabi NM, Sarhan NE, Elgarhy LH Tags: Ultrastruct Pathol Source Type: research

Ultrastructural changes of extraocular muscles in strabismus patients.
In conclusion, our study traces some characteristic ultrastructural changes in strabismic EOMs, most notably, extensive vacuolation, clustering of mitochondria, degeneration of myofibrils and autophagic changes. These changes might be emphasized as possibly secondary to strabismus. PMID: 31570030 [PubMed - as supplied by publisher] (Source: Ultrastructural Pathology)
Source: Ultrastructural Pathology - September 30, 2019 Category: Pathology Authors: Al-Falki Y, Al-Shraim M, Alsabaani NA, Eid RA, Radad K Tags: Ultrastruct Pathol Source Type: research

Metformin suppresses aortic ultrastrucural damage and hypertension induced by diabetes: a potential role of advanced glycation end products.
Abstract Cardiovascular disease secondary to diabetes represents a significant challenge to the health community. The advanced glycation end products (AGEs) play an important role in diabetes-mediated vascular injury. We tested whether metformin can suppress aortic AGEs production and protect against aortic injuries (aortopathy) and hypertension in streptozotocin-induced type 2 diabetes mellitus (T2DM) animal model. T2DM was induced in rats two weeks after being fed on a high carbohydrate and fat diet (HCFD), and continued on a HCFD until being sacrificed at week 12 (model group). The protective group was put on m...
Source: Ultrastructural Pathology - September 15, 2019 Category: Pathology Authors: Dallak M, Haidara MA, Bin-Jaliah I, Eid RA, Amin SN, Abdel Latif NS, Al-Ani B Tags: Ultrastruct Pathol Source Type: research

Vitamin E ameliorates alterations to the articular cartilage of knee joints induced by monoiodoacetate and diabetes mellitus in rats.
Abstract We recently reported an animal model of osteoarthritis (OA) induced by a combination of the chondrocyte glycolysis inhibitor, monoiodoacetate (MIA) and the agent that induces diabetes mellitus, streptozotocin (STZ). Here we investigated the potential protective effect of the antioxidant and anti-inflammatory agent, vitamin E against MIA+STZ-induced OA. Therefore, rats were either injected once with MIA (2 mg/50 μL) + 65 mg/kg STZ before being sacrificed after 8 weeks (model group) or were treated immediately after MIA+STZ injections with vitamin E (600 mg/kg; thrice a week) before being sacrificed afte...
Source: Ultrastructural Pathology - June 9, 2019 Category: Pathology Authors: Hassan WN, Bin-Jaliah I, Haidara MA, Eid RA, Heidar EHA, Dallak M, Al-Ani B Tags: Ultrastruct Pathol Source Type: research

One cell one niche: hematopoietic microenvironments constructed by bone marrow stromal cells with fibroblastic and histiocytic features.
Abstract Hematopoietic microenvironments have been extensively studied, especially focusing on regulation of hematopoietic stem cells (HSCs) in HSC niche following progress of molecular biology in resent years. Based on prior morphological achievements from 1970s, the characteristics of cellular compartments and bone marrow stromal cells (BMSCs) were studied ultrastructurally in human and mice bone marrow in the present study. The samples, human bone marrow granules, were collected from bone marrow aspirations (BMAs) of 20 patients with hematocytopenia and isolated BMSCs were found undesignedly in nucleated cells ...
Source: Ultrastructural Pathology - May 28, 2019 Category: Pathology Authors: Ru YX, Dong SX, Zhao SX, Li Y, Liang HY, Zhang MMF, Zhu X, Zheng Y Tags: Ultrastruct Pathol Source Type: research

Dusting off the numbers of in situ particle analysis: a 35-year experience.
We report herein our findings in 96 cases in which in situ particle analysis (ISPA) was performed with the SEM. This included 56 cases of pneumoconiosis as well as 40 cases of other types of interstitial lung disease. The most common diagnosis for which ISPA was performed in individuals with pneumoconiosis was mixed dust pneumoconiosis (14 cases). The most common diagnoses for which ISPA was performed in individuals with other diseases were sarcoidosis (13 cases) and hypersensitivity pneumonitis (10 cases). In addition to a detailed description of our methodology, we also report other circumstances in which ISPA assists in...
Source: Ultrastructural Pathology - May 21, 2019 Category: Pathology Authors: Carney JM, Pavlisko EN, Sporn TA, Roggli VL Tags: Ultrastruct Pathol Source Type: research

Correction.
Authors: PMID: 31064289 [PubMed - in process] (Source: Ultrastructural Pathology)
Source: Ultrastructural Pathology - May 10, 2019 Category: Pathology Tags: Ultrastruct Pathol Source Type: research

Impacts of resveratrol versus platelet-rich plasma for treatment of experimentally lithium-induced thyroid follicular cell toxicity in rats. A histological and immunohistochemical study.
This study aimed to assess the impacts of RSV versus PRP on lithium-induced thyroid follicular cell toxicity in adult male rats. Forty-nine adult male rats were divided into four groups: group I: control rats; group II: lithium-treated rats; group III: lithium- and resveratrol-treated rats; group IV: lithium and PRP-treated rats. Thyroid specimens were taken and processed for histological and immunohistochemical methods. Morphometrical studies and statistical analysis were done. Group II showed distorted thyroid follicles, significantly increased collagen fibers, and highly positive P53 immunostaining (P 
Source: Ultrastructural Pathology - April 13, 2019 Category: Pathology Authors: El-Mahalaway AM, El-Azab NE Tags: Ultrastruct Pathol Source Type: research

The ultrastructural effects of surgical treatment of hydrosalpinx on the human endometrium: a light and electron microscopic study.
In conclusion; serious structural changes have occurred in endometrial tissues of hydrosalpinx patients. These structural abnormalities have removed after surgical treatment so it is considered that surgical treatment is effective in patients with hydrosalpinx. PMID: 30966840 [PubMed - as supplied by publisher] (Source: Ultrastructural Pathology)
Source: Ultrastructural Pathology - April 9, 2019 Category: Pathology Authors: Sapmaz T, Gündoğdu LS, Çetin MT, Ürünsak IF, Polat S Tags: Ultrastruct Pathol Source Type: research

Coexistence of Hermansky-Pudlak syndrome and JAK2V617F-positive essential thrombocythemia.
Abstract Hermansky-Pudlak syndrome (HPS) is an autosomal recessive disorder consisting of oculocutaneous albinism, platelet storage pool deficiency, and lysosomal accumulation of ceroid lipofuscin. The storage pool deficiency of HPS is associated with the lack of dense bodies in the platelets, resulting in impaired response in the secondary phase of aggregation. Patients with HPS have normal coagulation tests; however, their bleeding time is usually prolonged despite normal or increased platelet counts. Essential thrombocythemia (ET) is an uncommon condition, with an incidence of approximately 1.1 per 100,000/year...
Source: Ultrastructural Pathology - April 1, 2019 Category: Pathology Authors: Eskazan T, Erturkuner SP, Isildar B, Eskazan AE, Ar MC, Atay K, Baslar Z, Tasyurekli M Tags: Ultrastruct Pathol Source Type: research

Protective effect of mirtazapine versus ginger against cisplatin-induced testicular damage in adult male albino rats.
This study examined the microscopic changes associated with CP and the possible testicular protective role of mirtazapine versus ginger of adult male rats. Mirtazapine and ginger resulted in cellular protection of testicular tissue as evident from microscopic changes including Sertoli cells, spermatogonia, and Leydig cells. Ginger showed to have a more protective effect than mirtazapine on testicular tissue against CP treatment. PMID: 30929557 [PubMed - as supplied by publisher] (Source: Ultrastructural Pathology)
Source: Ultrastructural Pathology - March 30, 2019 Category: Pathology Authors: Makary EFY, Rahman AA, Awny MM, Makary S Tags: Ultrastruct Pathol Source Type: research

Correlative polarizing light and scanning electron microscopy for the assessment of talc in pelvic region lymph nodes.
Abstract Perineal talc use is associated with ovarian carcinoma in many case-control studies. Such talc may migrate to pelvic organs and regional lymph nodes, with both clinical and legal significance. Our goal was to differentiate talc in pelvic lymph nodes due to exposure, versus contamination with talc in the laboratory. We studied 22 lymph nodes from ovarian tumor patients, some of which had documented talc exposure, to quantify talc using digestion of tissue taken from paraffin blocks and scanning electron microscopy/energy dispersive X-ray analysis (SEM/EDX). Talc particles correlated significantly with surf...
Source: Ultrastructural Pathology - March 21, 2019 Category: Pathology Authors: McDonald SA, Fan Y, Welch WR, Cramer DW, Stearns RC, Sheedy L, Katler M, Godleski JJ Tags: Ultrastruct Pathol Source Type: research

Electron microscopic findings suggestive of focal and segmental glomerulosclerosis in patients with steroid-resistant nephrotic syndrome.
This study was performed to compare electron microscopic findings in patients with steroid-resistant nephrotic syndrome with minimal histopathological abnormalities and cases with Focal Segmental Glomerulosclerosis. This Cohort study was conducted in Cancer Institute, Imam Khomeini Hospital Complex, Tehran, Iran. Twenty patients with steroid-resistant nephrotic syndrome and minimal changes on the light microscopic study were selected as case group. Similarly, 20 patients with Focal Segmental Glomerulosclerosis were selected as the control group. Ultrastructural findings were re-evaluated and scored qualitatively (0-3+). In...
Source: Ultrastructural Pathology - March 5, 2019 Category: Pathology Authors: Nili F, Saboori F, Jahanzad I, Mehrazma M Tags: Ultrastruct Pathol Source Type: research

Ultrastructural analysis of the Krebs-2 ascites cancer cells treated with extracellular double-stranded DNA preparation.
Abstract Electron-microscopic analysis of the ultrastructure of the Krebs-2 carcinoma ascites cells in the first 90 min immediately after their exposure to fragmented double-stranded DNA has been performed. Morphological attributes of the treated cancer cells indicate the induction in these cells of destructive processes of presumably apoptotic type. The predominance of dystrophic-destructive changes in cells after the addition of DNA is supposed to be a consequence of the disturbance in metabolic processes caused by the experimental action. PMID: 30758240 [PubMed - as supplied by publisher] (Source: Ultrastructural Pathology)
Source: Ultrastructural Pathology - February 13, 2019 Category: Pathology Authors: Kirikovich SS, Taranov OS, Omigov VV, Potter EA, Dolgova EV, Proskurina AS, Efremov YR, Bogachev SS Tags: Ultrastruct Pathol Source Type: research

Therapeutic effect of adipose-derived mesenchymal stem cells on Cisplatin induced testicular damage in adult male albino rat.
Abstract Infertility represents a major medical, economic, and psychological problem. Stem cells therapy for infertility has a great interest nowadays especially for cancer survivors at pre-reproductive and reproductive age.  Thirty-two adult male albino rats were used, divided equally into four groups; Group I (Control group) received isotonic saline intraperitoneally (i.p.) as vehicle. Group II (Cisplatin-treated group) received Cisplatin (i.p.) at a single dose of 7 mg/kg, and then were sacrificed after 5 days. Group III (Stem-cell-treated group) received Cisplatin (i.p.) at a single dose of 7 mg/kg, ...
Source: Ultrastructural Pathology - February 9, 2019 Category: Pathology Authors: Meligy FY, Abo Elgheed AT, Alghareeb SM Tags: Ultrastruct Pathol Source Type: research

Dimensions of elongated mineral particles: a study of more than 570 fibers from more than 90 cases with implications for pathogenicity and classification as asbestiform vs. cleavage fragments.
We examined more than 570 fibers from more than 90 cases to determine the dimensions of fibers that might be classified as asbestos. The vast majority of fibers classified as amosite or crocidolite met the criteria for length greater than 10 µm and diameter less than 1.0 µm. However, a significant proportion of fibers classified as tremolite, actinolite, or anthophyllite did not meet these criteria. These findings have important implications for the identification and classification of elongated mineral particles, both in terms of pathogenicity as well as classification as asbestiform vs. cleavage fra...
Source: Ultrastructural Pathology - January 22, 2019 Category: Pathology Authors: Roggli VL, Green CL Tags: Ultrastruct Pathol Source Type: research

Protective effect of tetrahydrobiopterin on hepatic and renal damage after acute cadmium exposure in male rats.
CONCLUSION: This study has provided clear evidence for the protective efficacy of BH4 against experimental Cd toxicity. PMID: 30595070 [PubMed - as supplied by publisher] (Source: Ultrastructural Pathology)
Source: Ultrastructural Pathology - December 29, 2018 Category: Pathology Authors: Mostafa DG, Ahmed SF, Hussein OA Tags: Ultrastruct Pathol Source Type: research

β-catenin and PPAR-γ levels in bone marrow of myeloproliferative neoplasm: an immunohistochemical and ultrastructural study.
β-catenin and PPAR-γ levels in bone marrow of myeloproliferative neoplasm: an immunohistochemical and ultrastructural study. Ultrastruct Pathol. 2018 Dec 24;:1-10 Authors: Subotički T, Mitrović Ajtić O, Mićić M, Kravić Stevović T, Đikić D, Diklić M, Leković D, Gotić M, Čokić VP Abstract In accordance with increased proliferation in myeloproliferative neoplasm (MPN), the goal is to evaluate the immunoexpression of: β-catenin, PPAR-γ and Ki67 protein, to compare them with bone marrow ultrastructural characteristics in patients with MPN. Immunoexpression and electron m...
Source: Ultrastructural Pathology - December 24, 2018 Category: Pathology Authors: Subotički T, Mitrović Ajtić O, Mićić M, Kravić Stevović T, Đikić D, Diklić M, Leković D, Gotić M, Čokić VP Tags: Ultrastruct Pathol Source Type: research