Approaches and challenges in genome-wide circular RNA identification and quantification
Trends Genet. 2023 Oct 13:S0168-9525(23)00223-8. doi: 10.1016/j.tig.2023.09.006. Online ahead of print.ABSTRACTNumerous circular RNAs (circRNAs) produced from back-splicing of exon(s) have been recently revealed on a genome-wide scale across species. Although generally expressed at a low level, some relatively abundant circRNAs can play regulatory roles in various biological processes, prompting continuous profiling of circRNA in broader conditions. Over the past decade, distinct strategies have been applied in both transcriptome enrichment and bioinformatic tools for detecting and quantifying circRNAs. Understanding the s...
Source: Trends in Genetics : TIG - October 15, 2023 Category: Genetics & Stem Cells Authors: Xu-Kai Ma Si-Nan Zhai Li Yang Source Type: research
Approaches and challenges in genome-wide circular RNA identification and quantification
Trends Genet. 2023 Oct 13:S0168-9525(23)00223-8. doi: 10.1016/j.tig.2023.09.006. Online ahead of print.ABSTRACTNumerous circular RNAs (circRNAs) produced from back-splicing of exon(s) have been recently revealed on a genome-wide scale across species. Although generally expressed at a low level, some relatively abundant circRNAs can play regulatory roles in various biological processes, prompting continuous profiling of circRNA in broader conditions. Over the past decade, distinct strategies have been applied in both transcriptome enrichment and bioinformatic tools for detecting and quantifying circRNAs. Understanding the s...
Source: Trends in Genetics : TIG - October 15, 2023 Category: Genetics & Stem Cells Authors: Xu-Kai Ma Si-Nan Zhai Li Yang Source Type: research
Approaches and challenges in genome-wide circular RNA identification and quantification
Trends Genet. 2023 Oct 13:S0168-9525(23)00223-8. doi: 10.1016/j.tig.2023.09.006. Online ahead of print.ABSTRACTNumerous circular RNAs (circRNAs) produced from back-splicing of exon(s) have been recently revealed on a genome-wide scale across species. Although generally expressed at a low level, some relatively abundant circRNAs can play regulatory roles in various biological processes, prompting continuous profiling of circRNA in broader conditions. Over the past decade, distinct strategies have been applied in both transcriptome enrichment and bioinformatic tools for detecting and quantifying circRNAs. Understanding the s...
Source: Trends in Genetics : TIG - October 15, 2023 Category: Genetics & Stem Cells Authors: Xu-Kai Ma Si-Nan Zhai Li Yang Source Type: research
Approaches and challenges in genome-wide circular RNA identification and quantification
Trends Genet. 2023 Oct 13:S0168-9525(23)00223-8. doi: 10.1016/j.tig.2023.09.006. Online ahead of print.ABSTRACTNumerous circular RNAs (circRNAs) produced from back-splicing of exon(s) have been recently revealed on a genome-wide scale across species. Although generally expressed at a low level, some relatively abundant circRNAs can play regulatory roles in various biological processes, prompting continuous profiling of circRNA in broader conditions. Over the past decade, distinct strategies have been applied in both transcriptome enrichment and bioinformatic tools for detecting and quantifying circRNAs. Understanding the s...
Source: Trends in Genetics : TIG - October 15, 2023 Category: Genetics & Stem Cells Authors: Xu-Kai Ma Si-Nan Zhai Li Yang Source Type: research
Approaches and challenges in genome-wide circular RNA identification and quantification
Trends Genet. 2023 Oct 13:S0168-9525(23)00223-8. doi: 10.1016/j.tig.2023.09.006. Online ahead of print.ABSTRACTNumerous circular RNAs (circRNAs) produced from back-splicing of exon(s) have been recently revealed on a genome-wide scale across species. Although generally expressed at a low level, some relatively abundant circRNAs can play regulatory roles in various biological processes, prompting continuous profiling of circRNA in broader conditions. Over the past decade, distinct strategies have been applied in both transcriptome enrichment and bioinformatic tools for detecting and quantifying circRNAs. Understanding the s...
Source: Trends in Genetics : TIG - October 15, 2023 Category: Genetics & Stem Cells Authors: Xu-Kai Ma Si-Nan Zhai Li Yang Source Type: research
Approaches and challenges in genome-wide circular RNA identification and quantification
Trends Genet. 2023 Oct 13:S0168-9525(23)00223-8. doi: 10.1016/j.tig.2023.09.006. Online ahead of print.ABSTRACTNumerous circular RNAs (circRNAs) produced from back-splicing of exon(s) have been recently revealed on a genome-wide scale across species. Although generally expressed at a low level, some relatively abundant circRNAs can play regulatory roles in various biological processes, prompting continuous profiling of circRNA in broader conditions. Over the past decade, distinct strategies have been applied in both transcriptome enrichment and bioinformatic tools for detecting and quantifying circRNAs. Understanding the s...
Source: Trends in Genetics : TIG - October 15, 2023 Category: Genetics & Stem Cells Authors: Xu-Kai Ma Si-Nan Zhai Li Yang Source Type: research
Approaches and challenges in genome-wide circular RNA identification and quantification
Trends Genet. 2023 Oct 13:S0168-9525(23)00223-8. doi: 10.1016/j.tig.2023.09.006. Online ahead of print.ABSTRACTNumerous circular RNAs (circRNAs) produced from back-splicing of exon(s) have been recently revealed on a genome-wide scale across species. Although generally expressed at a low level, some relatively abundant circRNAs can play regulatory roles in various biological processes, prompting continuous profiling of circRNA in broader conditions. Over the past decade, distinct strategies have been applied in both transcriptome enrichment and bioinformatic tools for detecting and quantifying circRNAs. Understanding the s...
Source: Trends in Genetics : TIG - October 15, 2023 Category: Genetics & Stem Cells Authors: Xu-Kai Ma Si-Nan Zhai Li Yang Source Type: research
Recent insights into eukaryotic double-strand DNA break repair unveiled by single-molecule methods
Trends Genet. 2023 Oct 6:S0168-9525(23)00221-4. doi: 10.1016/j.tig.2023.09.004. Online ahead of print.ABSTRACTGenome integrity and maintenance are essential for the viability of all organisms. A wide variety of DNA damage types have been described, but double-strand breaks (DSBs) stand out as one of the most toxic DNA lesions. Two major pathways account for the repair of DSBs: homologous recombination (HR) and non-homologous end joining (NHEJ). Both pathways involve complex DNA transactions catalyzed by proteins that sequentially or cooperatively work to repair the damage. Single-molecule methods allow visualization of the...
Source: Trends in Genetics : TIG - October 8, 2023 Category: Genetics & Stem Cells Authors: Sara De Bragan ça Mark S Dillingham Fernando Moreno-Herrero Source Type: research
Recent insights into eukaryotic double-strand DNA break repair unveiled by single-molecule methods
Trends Genet. 2023 Oct 6:S0168-9525(23)00221-4. doi: 10.1016/j.tig.2023.09.004. Online ahead of print.ABSTRACTGenome integrity and maintenance are essential for the viability of all organisms. A wide variety of DNA damage types have been described, but double-strand breaks (DSBs) stand out as one of the most toxic DNA lesions. Two major pathways account for the repair of DSBs: homologous recombination (HR) and non-homologous end joining (NHEJ). Both pathways involve complex DNA transactions catalyzed by proteins that sequentially or cooperatively work to repair the damage. Single-molecule methods allow visualization of the...
Source: Trends in Genetics : TIG - October 8, 2023 Category: Genetics & Stem Cells Authors: Sara De Bragan ça Mark S Dillingham Fernando Moreno-Herrero Source Type: research
Recent insights into eukaryotic double-strand DNA break repair unveiled by single-molecule methods
Trends Genet. 2023 Oct 6:S0168-9525(23)00221-4. doi: 10.1016/j.tig.2023.09.004. Online ahead of print.ABSTRACTGenome integrity and maintenance are essential for the viability of all organisms. A wide variety of DNA damage types have been described, but double-strand breaks (DSBs) stand out as one of the most toxic DNA lesions. Two major pathways account for the repair of DSBs: homologous recombination (HR) and non-homologous end joining (NHEJ). Both pathways involve complex DNA transactions catalyzed by proteins that sequentially or cooperatively work to repair the damage. Single-molecule methods allow visualization of the...
Source: Trends in Genetics : TIG - October 8, 2023 Category: Genetics & Stem Cells Authors: Sara De Bragan ça Mark S Dillingham Fernando Moreno-Herrero Source Type: research
Recent insights into eukaryotic double-strand DNA break repair unveiled by single-molecule methods
Trends Genet. 2023 Oct 6:S0168-9525(23)00221-4. doi: 10.1016/j.tig.2023.09.004. Online ahead of print.ABSTRACTGenome integrity and maintenance are essential for the viability of all organisms. A wide variety of DNA damage types have been described, but double-strand breaks (DSBs) stand out as one of the most toxic DNA lesions. Two major pathways account for the repair of DSBs: homologous recombination (HR) and non-homologous end joining (NHEJ). Both pathways involve complex DNA transactions catalyzed by proteins that sequentially or cooperatively work to repair the damage. Single-molecule methods allow visualization of the...
Source: Trends in Genetics : TIG - October 8, 2023 Category: Genetics & Stem Cells Authors: Sara De Bragan ça Mark S Dillingham Fernando Moreno-Herrero Source Type: research
Recent insights into eukaryotic double-strand DNA break repair unveiled by single-molecule methods
Trends Genet. 2023 Oct 6:S0168-9525(23)00221-4. doi: 10.1016/j.tig.2023.09.004. Online ahead of print.ABSTRACTGenome integrity and maintenance are essential for the viability of all organisms. A wide variety of DNA damage types have been described, but double-strand breaks (DSBs) stand out as one of the most toxic DNA lesions. Two major pathways account for the repair of DSBs: homologous recombination (HR) and non-homologous end joining (NHEJ). Both pathways involve complex DNA transactions catalyzed by proteins that sequentially or cooperatively work to repair the damage. Single-molecule methods allow visualization of the...
Source: Trends in Genetics : TIG - October 8, 2023 Category: Genetics & Stem Cells Authors: Sara De Bragan ça Mark S Dillingham Fernando Moreno-Herrero Source Type: research
Recent insights into eukaryotic double-strand DNA break repair unveiled by single-molecule methods
Trends Genet. 2023 Oct 6:S0168-9525(23)00221-4. doi: 10.1016/j.tig.2023.09.004. Online ahead of print.ABSTRACTGenome integrity and maintenance are essential for the viability of all organisms. A wide variety of DNA damage types have been described, but double-strand breaks (DSBs) stand out as one of the most toxic DNA lesions. Two major pathways account for the repair of DSBs: homologous recombination (HR) and non-homologous end joining (NHEJ). Both pathways involve complex DNA transactions catalyzed by proteins that sequentially or cooperatively work to repair the damage. Single-molecule methods allow visualization of the...
Source: Trends in Genetics : TIG - October 8, 2023 Category: Genetics & Stem Cells Authors: Sara De Bragan ça Mark S Dillingham Fernando Moreno-Herrero Source Type: research
Noncoding snoRNA host genes are a distinct subclass of long noncoding RNAs
Trends Genet. 2023 Sep 30:S0168-9525(23)00218-4. doi: 10.1016/j.tig.2023.09.001. Online ahead of print.ABSTRACTMammalian genomes are pervasively transcribed into different noncoding (nc)RNA classes, each one with its own hallmarks and exceptions. Some of them are nested into each other, such as host genes for small nucleolar RNAs (snoRNAs), which were long believed to simply act as molecular containers strictly facilitating snoRNA biogenesis. However, recent findings show that noncoding snoRNA host genes (ncSNHGs) display features different from those of 'regular' long ncRNAs (lncRNAs) and, more importantly, they can exert...
Source: Trends in Genetics : TIG - October 2, 2023 Category: Genetics & Stem Cells Authors: Alan Monziani Igor Ulitsky Source Type: research
Noncoding snoRNA host genes are a distinct subclass of long noncoding RNAs
Trends Genet. 2023 Sep 30:S0168-9525(23)00218-4. doi: 10.1016/j.tig.2023.09.001. Online ahead of print.ABSTRACTMammalian genomes are pervasively transcribed into different noncoding (nc)RNA classes, each one with its own hallmarks and exceptions. Some of them are nested into each other, such as host genes for small nucleolar RNAs (snoRNAs), which were long believed to simply act as molecular containers strictly facilitating snoRNA biogenesis. However, recent findings show that noncoding snoRNA host genes (ncSNHGs) display features different from those of 'regular' long ncRNAs (lncRNAs) and, more importantly, they can exert...
Source: Trends in Genetics : TIG - October 2, 2023 Category: Genetics & Stem Cells Authors: Alan Monziani Igor Ulitsky Source Type: research
