Opportunities and tradeoffs in single-cell transcriptomic technologies
Trends Genet. 2023 Nov 10:S0168-9525(23)00231-7. doi: 10.1016/j.tig.2023.10.003. Online ahead of print.ABSTRACTRecent technological and algorithmic advances enable single-cell transcriptomic analysis with remarkable depth and breadth. Nonetheless, a persistent challenge is the compromise between the ability to profile high numbers of cells and the achievement of full-length transcript coverage. Currently, the field is progressing and developing new and creative solutions that improve cellular throughput, gene detection sensitivity and full-length transcript capture. Furthermore, long-read sequencing approaches for single-c...
Source: Trends in Genetics : TIG - November 12, 2023 Category: Genetics & Stem Cells Authors: Matilde I Conte Azahara Fuentes-Trillo Cecilia Dom ínguez Conde Source Type: research
Strategies for dissecting the complexity of neurodevelopmental disorders
Trends Genet. 2023 Nov 8:S0168-9525(23)00237-8. doi: 10.1016/j.tig.2023.10.009. Online ahead of print.ABSTRACTNeurodevelopmental disorders (NDDs) are associated with a wide range of clinical features, affecting multiple pathways involved in brain development and function. Recent advances in high-throughput sequencing have unveiled numerous genetic variants associated with NDDs, which further contribute to disease complexity and make it challenging to infer disease causation and underlying mechanisms. Herein, we review current strategies for dissecting the complexity of NDDs using model organisms, induced pluripotent stem c...
Source: Trends in Genetics : TIG - November 10, 2023 Category: Genetics & Stem Cells Authors: Jiawan Sun Serena Noss Deepro Banerjee Maitreya Das Santhosh Girirajan Source Type: research
Retrotransposon renaissance in early embryos
Trends Genet. 2023 Nov 8:S0168-9525(23)00238-X. doi: 10.1016/j.tig.2023.10.010. Online ahead of print.ABSTRACTDespite being the predominant genetic elements in mammalian genomes, retrotransposons were often dismissed as genomic parasites with ambiguous biological significance. However, recent studies reveal their functional involvement in early embryogenesis, encompassing crucial processes such as zygotic genome activation (ZGA) and cell fate decision. This review underscores the paradigm shift in our understanding of retrotransposon roles during early preimplantation development, as well as their rich functional reservoir...
Source: Trends in Genetics : TIG - November 10, 2023 Category: Genetics & Stem Cells Authors: Youjia Guo Ten D Li Andrew J Modzelewski Haruhiko Siomi Source Type: research
Strategies for dissecting the complexity of neurodevelopmental disorders
Trends Genet. 2023 Nov 8:S0168-9525(23)00237-8. doi: 10.1016/j.tig.2023.10.009. Online ahead of print.ABSTRACTNeurodevelopmental disorders (NDDs) are associated with a wide range of clinical features, affecting multiple pathways involved in brain development and function. Recent advances in high-throughput sequencing have unveiled numerous genetic variants associated with NDDs, which further contribute to disease complexity and make it challenging to infer disease causation and underlying mechanisms. Herein, we review current strategies for dissecting the complexity of NDDs using model organisms, induced pluripotent stem c...
Source: Trends in Genetics : TIG - November 10, 2023 Category: Genetics & Stem Cells Authors: Jiawan Sun Serena Noss Deepro Banerjee Maitreya Das Santhosh Girirajan Source Type: research
Retrotransposon renaissance in early embryos
Trends Genet. 2023 Nov 8:S0168-9525(23)00238-X. doi: 10.1016/j.tig.2023.10.010. Online ahead of print.ABSTRACTDespite being the predominant genetic elements in mammalian genomes, retrotransposons were often dismissed as genomic parasites with ambiguous biological significance. However, recent studies reveal their functional involvement in early embryogenesis, encompassing crucial processes such as zygotic genome activation (ZGA) and cell fate decision. This review underscores the paradigm shift in our understanding of retrotransposon roles during early preimplantation development, as well as their rich functional reservoir...
Source: Trends in Genetics : TIG - November 10, 2023 Category: Genetics & Stem Cells Authors: Youjia Guo Ten D Li Andrew J Modzelewski Haruhiko Siomi Source Type: research
Pioneer factors: roles and their regulation in development
Trends Genet. 2023 Nov 6:S0168-9525(23)00235-4. doi: 10.1016/j.tig.2023.10.007. Online ahead of print.ABSTRACTPioneer factors are a subclass of transcription factors that can bind and initiate opening of silent chromatin regions. Pioneer factors subsequently regulate lineage-specific genes and enhancers and, thus, activate the zygotic genome after fertilization, guide cell fate transitions during development, and promote various forms of human cancers. As such, pioneer factors are useful in directed cell reprogramming. In this review, we define the structural and functional characteristics of pioneer factors, how they bind...
Source: Trends in Genetics : TIG - November 8, 2023 Category: Genetics & Stem Cells Authors: Amandine Barral Kenneth S Zaret Source Type: research
Pioneer factors: roles and their regulation in development
Trends Genet. 2023 Nov 6:S0168-9525(23)00235-4. doi: 10.1016/j.tig.2023.10.007. Online ahead of print.ABSTRACTPioneer factors are a subclass of transcription factors that can bind and initiate opening of silent chromatin regions. Pioneer factors subsequently regulate lineage-specific genes and enhancers and, thus, activate the zygotic genome after fertilization, guide cell fate transitions during development, and promote various forms of human cancers. As such, pioneer factors are useful in directed cell reprogramming. In this review, we define the structural and functional characteristics of pioneer factors, how they bind...
Source: Trends in Genetics : TIG - November 8, 2023 Category: Genetics & Stem Cells Authors: Amandine Barral Kenneth S Zaret Source Type: research
Pioneer factors: roles and their regulation in development
Trends Genet. 2023 Nov 6:S0168-9525(23)00235-4. doi: 10.1016/j.tig.2023.10.007. Online ahead of print.ABSTRACTPioneer factors are a subclass of transcription factors that can bind and initiate opening of silent chromatin regions. Pioneer factors subsequently regulate lineage-specific genes and enhancers and, thus, activate the zygotic genome after fertilization, guide cell fate transitions during development, and promote various forms of human cancers. As such, pioneer factors are useful in directed cell reprogramming. In this review, we define the structural and functional characteristics of pioneer factors, how they bind...
Source: Trends in Genetics : TIG - November 8, 2023 Category: Genetics & Stem Cells Authors: Amandine Barral Kenneth S Zaret Source Type: research
Making sense of proprioception
Trends Genet. 2023 Nov 3:S0168-9525(23)00234-2. doi: 10.1016/j.tig.2023.10.006. Online ahead of print.ABSTRACTProprioception - the sense of body position in space - is intimately linked to motor control. Here, we briefly review the current knowledge of the proprioceptive system and how advances in the genetic characterisation of proprioceptive sensory neurons in mice promise to dissect its role in health and disease.PMID:37926636 | DOI:10.1016/j.tig.2023.10.006 (Source: Trends in Genetics : TIG)
Source: Trends in Genetics : TIG - November 5, 2023 Category: Genetics & Stem Cells Authors: Alessandro Santuz Niccol ò Zampieri Source Type: research
Making sense of proprioception
Trends Genet. 2023 Nov 3:S0168-9525(23)00234-2. doi: 10.1016/j.tig.2023.10.006. Online ahead of print.ABSTRACTProprioception - the sense of body position in space - is intimately linked to motor control. Here, we briefly review the current knowledge of the proprioceptive system and how advances in the genetic characterisation of proprioceptive sensory neurons in mice promise to dissect its role in health and disease.PMID:37926636 | DOI:10.1016/j.tig.2023.10.006 (Source: Trends in Genetics : TIG)
Source: Trends in Genetics : TIG - November 5, 2023 Category: Genetics & Stem Cells Authors: Alessandro Santuz Niccol ò Zampieri Source Type: research
Making sense of proprioception
Trends Genet. 2023 Nov 3:S0168-9525(23)00234-2. doi: 10.1016/j.tig.2023.10.006. Online ahead of print.ABSTRACTProprioception - the sense of body position in space - is intimately linked to motor control. Here, we briefly review the current knowledge of the proprioceptive system and how advances in the genetic characterisation of proprioceptive sensory neurons in mice promise to dissect its role in health and disease.PMID:37926636 | DOI:10.1016/j.tig.2023.10.006 (Source: Trends in Genetics : TIG)
Source: Trends in Genetics : TIG - November 5, 2023 Category: Genetics & Stem Cells Authors: Alessandro Santuz Niccol ò Zampieri Source Type: research
Chromatin bridges: stochastic breakage or regulated resolution?
Trends Genet. 2023 Oct 25:S0168-9525(23)00232-9. doi: 10.1016/j.tig.2023.10.004. Online ahead of print.ABSTRACTGenetic material is organized in the form of chromosomes, which need to be segregated accurately into two daughter cells in each cell cycle. However, chromosome fusion or the presence of unresolved interchromosomal linkages lead to the formation of chromatin bridges, which can induce DNA lesions and genome instability. Persistent chromatin bridges are trapped in the cleavage furrow and are broken at or after abscission, the final step of cytokinesis. In this review, we focus on recent progress in understanding the...
Source: Trends in Genetics : TIG - October 27, 2023 Category: Genetics & Stem Cells Authors: Huadong Jiang Ying Wai Chan Source Type: research
Chromatin bridges: stochastic breakage or regulated resolution?
Trends Genet. 2023 Oct 25:S0168-9525(23)00232-9. doi: 10.1016/j.tig.2023.10.004. Online ahead of print.ABSTRACTGenetic material is organized in the form of chromosomes, which need to be segregated accurately into two daughter cells in each cell cycle. However, chromosome fusion or the presence of unresolved interchromosomal linkages lead to the formation of chromatin bridges, which can induce DNA lesions and genome instability. Persistent chromatin bridges are trapped in the cleavage furrow and are broken at or after abscission, the final step of cytokinesis. In this review, we focus on recent progress in understanding the...
Source: Trends in Genetics : TIG - October 27, 2023 Category: Genetics & Stem Cells Authors: Huadong Jiang Ying Wai Chan Source Type: research
Chromatin bridges: stochastic breakage or regulated resolution?
Trends Genet. 2023 Oct 25:S0168-9525(23)00232-9. doi: 10.1016/j.tig.2023.10.004. Online ahead of print.ABSTRACTGenetic material is organized in the form of chromosomes, which need to be segregated accurately into two daughter cells in each cell cycle. However, chromosome fusion or the presence of unresolved interchromosomal linkages lead to the formation of chromatin bridges, which can induce DNA lesions and genome instability. Persistent chromatin bridges are trapped in the cleavage furrow and are broken at or after abscission, the final step of cytokinesis. In this review, we focus on recent progress in understanding the...
Source: Trends in Genetics : TIG - October 27, 2023 Category: Genetics & Stem Cells Authors: Huadong Jiang Ying Wai Chan Source Type: research
Chromatin bridges: stochastic breakage or regulated resolution?
Trends Genet. 2023 Oct 25:S0168-9525(23)00232-9. doi: 10.1016/j.tig.2023.10.004. Online ahead of print.ABSTRACTGenetic material is organized in the form of chromosomes, which need to be segregated accurately into two daughter cells in each cell cycle. However, chromosome fusion or the presence of unresolved interchromosomal linkages lead to the formation of chromatin bridges, which can induce DNA lesions and genome instability. Persistent chromatin bridges are trapped in the cleavage furrow and are broken at or after abscission, the final step of cytokinesis. In this review, we focus on recent progress in understanding the...
Source: Trends in Genetics : TIG - October 27, 2023 Category: Genetics & Stem Cells Authors: Huadong Jiang Ying Wai Chan Source Type: research
