Metabolomic and lipidomic landscape of porcine kidney associated with kidney perfusion in heart beating donors and donors after cardiac death
Transplant centers are currently facing a lack of tools to ensure adequate evaluation of the quality of the available organs, as well as a significant shortage of kidney donors. Therefore, efforts are being made to facilitate the effective use of available organs and expand the donor pool, particularly with expanded criteria donors. Fulfilling a need, we aim to present an innovative analytical method based on solid-phase microextraction (SPME) - chemical biopsy. In order to track changes affecting the organ throughout the entire transplant procedure, porcine kidneys were subjected to multiple samplings at various time poin...
Source: Translational Research - December 2, 2023 Category: Research Authors: Iga Stryjak, Natalia Warmuzi ńska, Kamil Łuczykowski, Karol Jaroch, Peter Urbanellis, Markus Selzner, Barbara Bojko Source Type: research
Crosstalk between GABAA receptors in astrocytes and neurons triggered by general anesthetic drugs
General anesthetic drugs cause cognitive deficits that persist after the drugs have been eliminated. Astrocytes may contribute to such cognition-impairing effects through the release of one or more paracrine factors that increase a tonic inhibitory conductance generated by extrasynaptic γ-aminobutyric acid type A (GABAA) receptors in hippocampal neurons. The mechanisms underlying this astrocyte-to-neuron crosstalk remain unknown. Interestingly, astrocytes express anesthetic-sensitive GABAA receptors. (Source: Translational Research)
Source: Translational Research - November 30, 2023 Category: Research Authors: Dian-Shi Wang, Li Ju, Ars ène G. Pinguelo, Kirusanthy Kaneshwaran, Sean C. Haffey, Irene Lecker, Himaben Gohil, Michael B. Wheeler, Lilia Kaustov, Anthony Ariza, MeiFeng Yu, Allen Volchuk, Benjamin E. Steinberg, Neil M. Goldenberg, Beverley A. Orser Source Type: research
Efficacy of oral manganese and D-galactose therapy in a patient bearing a novel TMEM165 variant
TMEM165-CDG has first been reported in 2012 and manganese supplementation was shown highly efficient in rescuing glycosylation in isogenic KO cells. The unreported homozygous missense c.928G>C; p.Ala310Pro variant leading to a functional but unstable protein was identified. This patient was diagnosed at 2 months and displays a predominant bone phenotype and combined defects in N-, O- and GAG glycosylation. We administered for the first time a combined D-Gal and Mn2+ therapy to the patient. This fully suppressed the N-; O- and GAG hypoglycosylation. (Source: Translational Research)
Source: Translational Research - November 25, 2023 Category: Research Authors: Zo é Durin, Alexandre Raynor, François Fenaille, Sophie Cholet, Sandrine Vuillaumier-Barrot, Jean-Meidi Alili, Joël Poupon, Nouzha Djebrani Oussedik, Caroline Tuchmann-Durand, Jennifer Attali, Romain Touzé, Thierry Dupré, Elodie Lebredonchel, Marlyse Source Type: research
Metadherin orchestrates PKA and PKM2 to activate β-catenin signaling in podocytes during proteinuric chronic kidney disease
Podocyte damage is the major cause of glomerular injury and proteinuria in multiple chronic kidney diseases. Metadherin (MTDH) is involved in podocyte apoptosis and promotes renal tubular injury in mouse models of diabetic nephropathy and renal fibrosis; however, its role in podocyte injury and proteinuria needs further exploration. Here, we show that MTDH was induced in the glomerular podocytes of patients with proteinuric chronic kidney disease and correlated with proteinuria. Podocyte-specific knockout of MTDH in mice reversed proteinuria, attenuated podocyte injury, and prevented glomerulosclerosis after advanced oxida...
Source: Translational Research - November 21, 2023 Category: Research Authors: Xiaowen Chen, Jing Xiao, Danping Tao, Yunyi Liang, Sijia Chen, Lingyu Shen, Shuting Li, Zerong Zheng, Yao Zeng, Congwei Luo, Fenfen Peng, Haibo Long Source Type: research
Knockdown of ADAM8 inhibits the proliferation, migration, invasion, and tumorigenesis of renal clear cell carcinoma cells to enhance the immunotherapy efficacy
The current study performed bioinformatics and in vitro and in vivo experiments to explore the effects of ADAM8 on the malignant behaviors and immunotherapeutic efficacy of renal clear cell carcinoma (ccRCC) Cells. The modular genes most associated with immune cells were screened. Then, prognostic risk models were constructed by univariate COX analysis, LASSO regression analysis and multivariate COX analysis, and their diagnostic value was determined. The correlation between tumor mutation load (TMB) scores and the prognosis of ccRCC patients was clarified. (Source: Translational Research)
Source: Translational Research - November 20, 2023 Category: Research Authors: Hongchen Qu, Minghuan Mao, Kai Wang, Zhongyi Mu, Bin Hu Source Type: research
High Comorbidity of Pediatric Cancers in Patients with Birth Defects: Insights from Whole Genome Sequencing Analysis of Copy Number Variations
Patients with birth defects (BD) exhibit an elevated risk of cancer. We aimed to investigate the potential link between pediatric cancers and BDs, exploring the hypothesis of shared genetic defects contributing to the coexistence of these conditions. (Source: Translational Research)
Source: Translational Research - November 19, 2023 Category: Research Authors: Hui-Qi Qu, Joseph T. Glessner, Jingchun Qu, Yichuan Liu, Deborah Watson, Xiao Chang, Amir Hossein Saeidian, Haijun Qiu, Frank D Mentch, John J Connolly, Hakon Hakonarson Source Type: research
Current updates on metabolites and its interlinked pathways as biomarkers for diabetic kidney disease: a systematic review
Diabetic kidney disease (DKD) is a major microvascular complication of diabetes mellitus (DM) that poses a serious risk as it can lead to end-stage renal disease (ESRD). DKD is linked to changes in the diversity, composition, and functionality of the microbiota present in the gastrointestinal tract. The interplay between the gut microbiota and the host organism is primarily facilitated by metabolites generated by microbial metabolic processes from both dietary substrates and endogenous host compounds. (Source: Translational Research)
Source: Translational Research - November 10, 2023 Category: Research Authors: Soumik Das, Devi Rajeswari, Ganesh Venkataraman, Ramprasad Elumalai, Sivaraman Dhanasekaran, Gnanasambandan Ramanathan Tags: Review Article Source Type: research
Tumor molecular landscape of Epstein-Barr virus (EBV) related nasopharyngeal carcinoma in EBV-endemic and non-endemic areas: implications for improving treatment modalities
Epstein-Barr virus (EBV) related- nasopharyngeal carcinoma (NPC) is a squamous carcinoma of the nasopharyngeal mucosal lining. Endemic areas (EA) are east and Southeast Asia, were NPC was recorded with higher incidence and longer estimated survival than in non-endemic area (NEA) such as Europe, We analyzed the gene expression and microenvironment properties of NPC in both areas to identify molecular subtypes and assess biological and clinical correlates that might explain the differences in incidence and outcome between EA- and NEA-NPCs. (Source: Translational Research)
Source: Translational Research - November 7, 2023 Category: Research Authors: Deborah Lenoci, Carlo Resteghini, Mara S. Serafini, Federico Pistore, Silvana Canevari, Brigette Ma, Stefano Cavalieri, Salvatore Alfieri, Annalisa Trama, Lisa Licitra, Loris De Cecco Source Type: research
Integrin β8 prevents pericyte-myofibroblast transition and renal fibrosis through inhibiting the TGF-β1/TGFBR1/Smad3 pathway in diabetic kidney disease
Diabetic kidney disease (DKD) is one of the leading causes to develop end-stage kidney disease worldwide. Pericytes are implicated in the development of tissue fibrosis. However, the underlying mechanisms of pericytes in DKD remain largely unknown. We isolated and cultured primary pericytes and rat mesangial cells (HBZY-1). Western blot and qRT-PCR analysis were used to explore the role and regulatory mechanism of Integrin β8/transforming growth factor beta 1 (TGF-β1) pathway. We also constructed pericyte-specific Integrin β8 knock-in mice as the research objects to determine the role of Integrin β8 in vivo. (Source: ...
Source: Translational Research - November 3, 2023 Category: Research Authors: Yiling Cao, Hua Su, Jieyu Zeng, Yaru Xie, Zezhou Liu, Feng Liu, Yang Qiu, Fan Yi, Jihong Lin, Hans-Peter Hammes, Chun Zhang Source Type: research
TRPV4 in adipose tissue ameliorates diet-induced obesity by promoting white adipocyte browning
This study revealed the role of TRPV4 in adipose tissue in the development of obesity. Adipose-specific TRPV4 overexpression protected mice against diet-induced obesity (DIO) and promoted white fat browning. (Source: Translational Research)
Source: Translational Research - November 3, 2023 Category: Research Authors: Yan Zhang, Jie Xue, Wenjuan Zhu, Haomin Wang, Pengjiao Xi, Derun Tian Source Type: research
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