Polymerized human hemoglobin with low and high oxygen affinity in trauma models
The present study aimed to compare the ability of tense (T) and relax R-state polymerized human Hemoglobin (PolyhHbs) to restore hemodynamics after severe trauma in a rat model, and to assess their relative toxicity in a guinea pigs (GPs). To assess the efficacy of these PolyhHbs in restoring hemodynamics, Wistar rats were subjected to traumatic brain injury (TBI) followed by hemorrhagic shock (HS). Animals were separated into 3 groups based on the resuscitation solution: Whole Blood, T-state or R-state PolyhHb, and followed for 2 hours after resuscitation. (Source: Translational Research)
Source: Translational Research - May 31, 2023 Category: Research Authors: Cynthia R. Muller, Vasiliki Courelli, Cynthia Walser, Clayton T. Cuddington, Savannah R. Wolfe, Andre F. Palmer, Pedro Cabrales Source Type: research
Norcantharidin potentiates sorafenib antitumor activity in hepatocellular carcinoma rat model through inhibiting IL-6/STAT3 pathway
In hepatocellular carcinoma (HCC), sorafenib (Sora) efficacy is limited by primary and/or acquired resistance. Emerging evidence shows that the inflammatory factor interleukin 6 (IL-6) plays a role in Sora resistance. Norcantharidin (NCTD), a derivative of cantharidine, was identified as a potent IL-6 inhibitor. Thus, in this study, we evaluated NCTD ability to improve the Sora efficacy in HCC and its underlying molecular mechanisms. Male Sprague Dawely rats were administered NCTD (0.1 mg/kg/day; orally) or Sora (10 mg/kg day; orally) or combination for 6 weeks after HCC induction using thioacetamide (200 mg/kg; ip; 2 time...
Source: Translational Research - May 29, 2023 Category: Research Authors: Eman H. Yousef, Nada F. Abo El-Magd, Amal M. El Gayar Source Type: research
Microsatellite instability in nonendometrioid ovarian epithelial tumors: a study of 400 cases comparing immunohistochemistry, PCR, and NGS based testing with mutation status of MMR genes
Testing of microsatellite instability is not only used as a triage for possible Lynch syndrome, but also to predict immunotherapy treatment response. The aim of this study was to assess the frequency of mismatch repair deficiency (MMR-D)/microsatellite instability (MSI) in 400 cases of nonendometrioid ovarian tumors (high-grade serous, low-grade serous, mucinous and clear cell), to compare different methodological approaches of testing, and to assess the optimal approach for next generation sequencing (NGS) MSI testing. (Source: Translational Research)
Source: Translational Research - May 24, 2023 Category: Research Authors: Nikola H ájková, Michaela Kendall Bártů, David Cibula, Jana Drozenová, Pavel Fabian, Oluwole Fadare, Filip Frühauf, Jitka Hausnerová, Jan hojný, Eva Krkavcová, Jan Laco, Sigurd F. Lax, Radoslav Matěj, Gábor Méhes, Romana Michálková, Kristýn Source Type: research
Microsatellite Instability in Non-Endometrioid Ovarian Epithelial Tumors: A study of 400 cases Comparing Immunohistochemistry, PCR, and NGS Based Testing with Mutation Status of MMR Genes.
Testing of microsatellite instability is not only used as a triage for possible Lynch syndrome, but also to predict immunotherapy treatment response. The aim of this study was to assess the frequency of mismatch repair deficiency (MMR-D) / microsatellite instability (MSI) in 400 cases of non-endometrioid ovarian tumors (high-grade serous, low-grade serous, mucinous and clear cell), to compare different methodological approaches of testing, and to assess the optimal approach for next generation sequencing (NGS) MSI testing. (Source: Translational Research)
Source: Translational Research - May 24, 2023 Category: Research Authors: Nikola H ájková, Michaela Kendall Bártů, David Cibula, Jana Drozenová, Pavel Fabian, Oluwole Fadare, Filip Frühauf, Jitka Hausnerová, Jan Hojný, Eva Krkavcová, Jan Laco, Sigurd F. Lax, Radoslav Matěj, Gábor Méhes, Romana Michálková, Kristýn Source Type: research
Diet-induced obesity augments ischemic myopathy and functional decline in a murine model of Peripheral Artery Disease
Peripheral artery disease (PAD) causes an ischemic myopathy contributing to patient disability and mortality. Most preclinical models to date use young, healthy rodents with limited translatability to human disease. Although PAD incidence increases with age, and obesity is a common comorbidity, the pathophysiologic association between these risk factors and PAD myopathy is unknown. Using our murine model of PAD, we sought to elucidate the combined effect of age, diet-induced obesity and chronic hindlimb ischemia (HLI) on 1) mobility, 2) muscle contractility, and markers of muscle 3) mitochondrial content and function, 4) o...
Source: Translational Research - May 21, 2023 Category: Research Authors: Emma Fletcher, Dimitrios Miserlis, Kristina Sorokolet, Dylan Wilburn, Cassandra Bradley, Evlampia Papoutsi, Trevor Wilkinson, Andrew Ring, Lucas Ferrer, Gleb Haynatzki, Robert S. Smith, William T Bohannon, Panagiotis Koutakis Source Type: research
Glut10 restrains neointima formation by promoting SMCs mtDNA demethylation and improving mitochondrial function
Neointimal hyperplasia is a major clinical complication of coronary artery bypass graft and percutaneous coronary intervention. Smooth muscle cells (SMCs) plays a vital roles in neointimal hyperplasia development and undergo complex phenotype switching. Previous studies have linked glucose transporter member 10(Glut10) to the phenotypic transformation of SMCs. In this research, we reported that Glut10 helps maintaining the contractile phenotype of SMCs. The Glut10-TET2/3 signaling axis can arrest neointimal hyperplasia progression by improving mitochondrial function via promotion of mtDNA demethylation in SMCs. (Source: Tr...
Source: Translational Research - May 21, 2023 Category: Research Authors: Qi Wu, Zhipeng Hu, Zhiwei Wang, Yanjia Che, Min Zhang, Sihao Zheng, Kai Xing, Xiaohan Zhong, Yuanyang Chen, Feng Shi, Shun Yuan Source Type: research
GABAergic signaling abnormalities in a novel CLU mutation Alzheimer's disease mouse model
The CLU rs11136000C mutation (CLUC) is the third most common risk factor for Alzheimer's disease (AD). However, the mechanism by which CLUC leads to abnormal GABAergic signaling in AD is unclear. To address this question, this study establishes the first chimeric mouse model of CLUC AD. Examination of grafted CLUC medial ganglionic eminence progenitors (CLUC hiMGEs) revealed increased GAD65/67 and a high frequency of spontaneous releasing events. CLUC hiMGEs also impaired cognition in chimeric mice and caused AD-related pathologies. (Source: Translational Research)
Source: Translational Research - May 19, 2023 Category: Research Authors: Chunxia Chen, Xihe Tang, Zhaohui Lan, Wan Chen, Hua Su, Weidong Li, Yaoxuan Li, Xing Zhou, Hong Gao, Xinwei Feng, Ying Guo, Meicun Yao, Wenbin Deng Source Type: research
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Source: Translational Research - May 14, 2023 Category: Research Source Type: research
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Source: Translational Research - May 14, 2023 Category: Research Source Type: research
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Source: Translational Research - May 14, 2023 Category: Research Source Type: research
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Source: Translational Research - May 14, 2023 Category: Research Source Type: research
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Source: Translational Research - May 14, 2023 Category: Research Source Type: research
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(Source: Translational Research)
Source: Translational Research - May 14, 2023 Category: Research Source Type: research
ZBTB7C m6A modification incurred by METTL3 aberration promotes osteosarcoma progression
Aberrant N6-methyladenosine (m6A) modification of mRNAs contributes significantly to the epigenetic tumorigenesis, however, its precise role and the key targets in osteosarcoma (OS) are not defined. Here we reported that selective METTL3 (methyltransferase like 3) elevation and the consequential increase of m6A modification causally affect OS progression. The fast-growing OS cells displayed preferential upregulation of METTL3 and increased m6A modification. Conversely, m6A inhibition by 3-deazaadenosine, siRNA-mediated METTL3 knockdown or a METTL3-selective inhibitor by STM2457 effectively inhibits OS cell growth and induc...
Source: Translational Research - April 28, 2023 Category: Research Authors: Xueying An, Wenshu Wu, Lin Yang, Jian Dong, Bin Liu, Junxia Guo, Jianmei Chen, Baosheng Guo, Wangsen Cao, Qing Jiang Source Type: research
A key role for NLRP3 signaling in preterm labor and birth driven by the alarmin S100B
Preterm birth remains the leading cause of neonatal morbidity and mortality worldwide. A substantial number of spontaneous preterm births occur in the context of sterile intra-amniotic inflammation, a condition that has been mechanistically proven to be triggered by alarmins. However, sterile intra-amniotic inflammation still lacks treatment. The NLRP3 inflammasome has been implicated in sterile intra-amniotic inflammation; yet, its underlying mechanisms, as well as the maternal and fetal contributions to this signaling pathway, are unclear. (Source: Translational Research)
Source: Translational Research - April 28, 2023 Category: Research Authors: Jose Galaz, Kenichiro Motomura, Roberto Romero, Zhenjie Liu, Valeria Garcia-Flores, Li Tao, Yi Xu, Bogdan Done, Marcia Arenas-Hernandez, Tomi Kanninen, Marcelo Farias-Jofre, Derek Miller, Adi L. Tarca, Nardhy Gomez-Lopez Source Type: research
