Translational Research : the journal of laboratory and clinical medicine This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Chronic kidney disease and left ventricular diastolic dysfunction (CKD-LVDD) alter cardiac expression of mitochondria-related genes in swine
Transl Res. 2024 Jan 22:S1931-5244(23)00205-0. doi: 10.1016/j.trsl.2023.12.004. Online ahead of print.ABSTRACTCardiovascular disease and heart failure doubles in patients with chronic kidney disease (CKD), but the underlying mechanisms remain obscure. Mitochondria are central to maintaining cellular respiration and modulating cardiomyocyte function. We took advantage of our novel swine model of CKD and left ventricular diastolic dysfunction (CKD-LVDD) to investigate the expression of mitochondria-related genes and potential mechanisms regulating their expression. CKD-LVDD and normal control pigs (n=6/group, 3 males/3 femal...
Source: Translational Research : the journal of laboratory and clinical medicine - January 23, 2024 Category: Laboratory Medicine Authors: Alejandro R Chade Rhys Sitz Taylor J Kelty Elizabeth McCarthy Darla L Tharp R Scott Rector Alfonso Eirin Source Type: research

Enhancing axonal myelination: Clemastine attenuates cognitive impairment in a rat model of diffuse traumatic brain injury
Transl Res. 2024 Jan 19:S1931-5244(24)00018-5. doi: 10.1016/j.trsl.2024.01.008. Online ahead of print.ABSTRACTTraumatic brain injury (TBI) has a significant impact on cognitive function, affecting millions of people worldwide. Myelin loss is a prominent pathological feature of TBI, while well-functioning myelin is crucial for memory and cognition. Utilizing drug repurposing to identify effective drug candidates for TBI treatment has gained attention. Notably, recent research has highlighted the potential of clemastine, an FDA-approved allergy medication, as a promising pro-myelinating drug. Therefore, in this study, we aim...
Source: Translational Research : the journal of laboratory and clinical medicine - January 21, 2024 Category: Laboratory Medicine Authors: Zhihai Huang Yu Feng Yulan Zhang Xiaohui Ma Xuemei Zong J Dedrick Jordan Quanguang Zhang Source Type: research

Astrocyte-derived exosomes-transported miRNA-26a-5p ameliorates sevoflurane-induced cognitive dysfunction in aged mice
Transl Res. 2024 Jan 19:S1931-5244(24)00017-3. doi: 10.1016/j.trsl.2024.01.007. Online ahead of print.ABSTRACTProlonged sevoflurane anesthesia is the primary factor contributing to the development of perioperative neurocognitive disorders (PND). Recent studies have highlighted neuronal apoptosis and abnormal dendritic structures as crucial features of PND. Astrocytes-derived exosomes (ADEs) have been identified as carriers of microRNAs (miRNAs), playing a vital role in cell-to-cell communication through transmitting genetic material. Nevertheless, the specific mechanisms by which miRNAs in ADEs contribute to sevoflurane-in...
Source: Translational Research : the journal of laboratory and clinical medicine - January 21, 2024 Category: Laboratory Medicine Authors: Junhua Li Hui Xu Kun Zhang Yafang Liu Cong Zeng Yanni Fu Yujuan Li Source Type: research

Active AKT2 stimulation of SREBP1/SCD1-mediated lipid metabolism boosts hepatosteatosis and cancer
In this study, we demonstrated that high-fat diet potentiated mouse liver AKT2. Hepatic AKT2 hyperactivation through gain-of-function mutation of Akt2 (Akt2E17K) caused spontaneous hepatosteatosis, injury, inflammation, fibrosis, and eventually HCC in mice. AKT2 activation also exacerbated lipopolysaccharide and D-galactosamine hydrochloride-induced injury/inflammation and N-Nitrosodiethylamine (DEN)-induced HCC. A positive correlation between AKT2 activity and SCD1 expression was observed in human HCC samples. Activated AKT2 enhanced the production of monounsaturated fatty acid which was dependent on SREBP1 upregulation o...
Source: Translational Research : the journal of laboratory and clinical medicine - January 20, 2024 Category: Laboratory Medicine Authors: Fuqiang Huang Na Zhao Pei Cai Mengjie Hou Shuhui Yang Bohao Zheng Qian Ma Jingpeng Jiang Xiaochen Gai Yilei Mao Lianmei Wang Zhongdong Hu Xiaojun Zha Fangming Liu Hongbing Zhang Source Type: research

Interleukin-33/ST2 axis involvement in atrial remodeling and arrhythmogenesis
This study explored the pathological effects of the IL-33/ST2 axis on atrial remodeling and arrhythmogenesis. Patch clamping, confocal microscopy, and Western blotting were used to analyze the electrical characteristics of and protein activity in atrial myocytes (HL-1) treated with recombinant IL-33 protein and/or ST2-neutralizing antibodies for 48 hrs. Telemetric electrocardiographic recordings, Masson's trichrome staining, and immunohistochemistry staining of the atrium were performed in mice receiving tail vein injections with nonspecific immunoglobulin (control), IL-33, and IL-33 combined with anti-ST2 antibody for 2 w...
Source: Translational Research : the journal of laboratory and clinical medicine - January 20, 2024 Category: Laboratory Medicine Authors: Tzu-Yu Cheng Yao-Chang Chen Shao-Jung Li Fong-Jhih Lin Yen-Yu Lu Ting-I Lee Ting-Wei Lee Satoshi Higa Yu-Hsun Kao Yi-Jen Chen Source Type: research

Cardiac-specific overexpression of CREM-Ib ΔC-X via CRISPR/Cas9 in mice presents a new model of atrial cardiomyopathy with spontaneous atrial fibrillation
Transl Res. 2024 Jan 8:S1931-5244(24)00011-2. doi: 10.1016/j.trsl.2024.01.001. Online ahead of print.ABSTRACTAtrial cardiomyopathy (ACM) forms the substrate for atrial fibrillation (AF) and underlies the potential for atrial thrombus formation and subsequent stroke. However, generating stable animal models that accurately replicate the entire progression of atrial lesions, particularly the onset of AF, presents significant challenges. In the present study, we found that the isoform of CRE-binding protein modulator (CREM-IbΔC-X), which is involved in the regulation of cardiac development and atrial rhythm, was highly expre...
Source: Translational Research : the journal of laboratory and clinical medicine - January 10, 2024 Category: Laboratory Medicine Authors: Jiuzhou Chen Huiyuan Qin Jingzhe Hao Qing Wang Shaojie Chen Gang Yang Mingfang Li Xiyu Zhu Dongjin Wang Hongwu Chen Chang Cui Minglong Chen Source Type: research

Heterogeneous nuclear ribonucleoprotein F deficiency in mouse podocyte promotes podocytopathy mediated by methyltransferase-like 14 nuclear translocation resulting in Sirtuin 1 gene inhibition
Conclusion: HnRNP F deficiency in podocytes promotes podocytopathy through activation of Mettl14 expression and its nuclear translocation to inhibit Sirt1 expression, underscoring the protective role of HnRNP F against podocyte injury.PMID:38195017 | DOI:10.1016/j.trsl.2024.01.002 (Source: Translational Research : the journal of laboratory and clinical medicine)
Source: Translational Research : the journal of laboratory and clinical medicine - January 9, 2024 Category: Laboratory Medicine Authors: Min-Chun Liao Chao-Sheng Lo Yu-Chao Pang Wen-Xia Yang Ke Su Xin-Ping Zhao Kana N Miyata Junzheng Peng Julie R Ingelfinger John S D Chan Shao-Ling Zhang Source Type: research

Heterogeneous Nuclear Ribonucleoprotein F Deficiency in Mouse Podocyte Promotes Podocytopathy Mediated by Methyltransferase-like 14 Nuclear Translocation Resulting in Sirtuin-1 Gene Inhibition
Conclusion: HnRNP F deficiency in podocytes promotes podocytopathy through activation of Mettl14 expression and its nuclear translocation to inhibit Sirt1 expression, underscoring the protective role of HnRNP F against podocyte injury.PMID:38195017 | DOI:10.1016/j.trsl.2024.01.002 (Source: Translational Research : the journal of laboratory and clinical medicine)
Source: Translational Research : the journal of laboratory and clinical medicine - January 9, 2024 Category: Laboratory Medicine Authors: Min-Chun Liao Chao-Sheng Lo Yu-Chao Pang Wen-Xia Yang Ke Su Xin-Ping Zhao Kana N Miyata Junzheng Peng Julie R Ingelfinger John S D Chan Shao-Ling Zhang Source Type: research

Aldehyde Dehydrogenase 2 Serves as a Key Cardiometabolic Adaptation Regulator in Response to Plateau Hypoxia in Mice
This study aims to determine the effects of plateau hypoxia (PH) on cardiac metabolism and function, investigate the associated role of ALDH2, and explore potential therapeutic targets. We discovered that PH significantly reduced survival rate and cardiac function. These effects were exacerbated by ALDH2 deficiency. PH also caused a shift in the myocardial fuel source from fatty acids to glucose; ALDH2 deficiency impaired this adaptive metabolic shift. Untargeted/targeted metabolomics and transmission electron microscopy revealed that ALDH2 deficiency promoted myocardial fatty-acid deposition, leading to enhanced fatty-aci...
Source: Translational Research : the journal of laboratory and clinical medicine - January 5, 2024 Category: Laboratory Medicine Authors: Rifeng Gao Kun Yang Shiguan Le Hanchuan Chen Xiaolei Sun Zhen Dong Pingjin Gao Xilu Wang Jiaran Shi Yanan Qu Xiang Wei Kai Hu Jiucun Wang Li Jin Yi Li Junbo Ge Aijun Sun Source Type: research

Metabolomic and lipidomic landscape of porcine kidney associated with kidney perfusion in heart beating donors and donors after cardiac death
Transl Res. 2023 Dec 3:S1931-5244(23)00202-5. doi: 10.1016/j.trsl.2023.12.001. Online ahead of print.ABSTRACTTransplant centers are currently facing a lack of tools to ensure adequate evaluation of the quality of the available organs, as well as a significant shortage of kidney donors. Therefore, efforts are being made to facilitate the effective use of available organs and expand the donor pool, particularly with expanded criteria donors. Fulfilling a need, we aim to present an innovative analytical method based on solid-phase microextraction (SPME) - chemical biopsy. In order to track changes affecting the organ througho...
Source: Translational Research : the journal of laboratory and clinical medicine - December 5, 2023 Category: Laboratory Medicine Authors: Iga Stryjak Natalia Warmuzi ńska Kamil Łuczykowski Karol Jaroch Peter Urbanellis Markus Selzner Barbara Bojko Source Type: research