Molecular basis of DEL phenotype in the Indian population - Insights from Next-Generation Sequencing Analysis of two cases
The DEL phenotype represents an intriguing and challenging aspect of blood group serology. This condition is characterized by an extremely weak expression of the D antigen on red blood cells, to the extent that it often eludes detection through routine serological methods. Identifying the DEL phenotype necessitates more specialized techniques, such as adsorption and elution tests, to reveal the presence of the D antigen. This distinctive phenotype underscores the complexity and subtlety of blood group genetics and highlights the importance of using advanced methods to accurately classify individuals with this condition, as...
Source: Transfusion and Apheresis Science - January 21, 2024 Category: Hematology Authors: Mercy Rophina, Ayesha Sinha, Durba Biswas, Debapriya Basu, Suvro Sankha Datta, Vinod Scaria Source Type: research

New threats from an old foe: evaluating the risk to the blood supply due to increasing incidence and endemicity of leprosy in the United States
Leprosy (i.e., Hansen ’s disease) is a chronic disease secondary to infection with either Mycobacterium leprae or M. lepromatosis. While the incidence of this disease is decreasing across the world, there is mounting evidence that it might be increasing, and becoming endemic, in the United States. Leprosy was once cons idered a potential threat to the blood supply, and while this threat has not borne out, it is worth revisiting the available data to assess whether it may pose a threat in the future. (Source: Transfusion and Apheresis Science)
Source: Transfusion and Apheresis Science - January 12, 2024 Category: Hematology Authors: Jeremy W. Jacobs, Brian D. Adkins, Lorin A. Bibb, Laura D. Stephens, Jennifer S. Woo, Evan M. Bloch, Garrett S. Booth Source Type: research

In-house RT-PCR detection of hepatitis A virus in human plasma pools used for the manufacture of medicinal products
Hepatitis A virus (HAV) is the most frequent cause of viral hepatitis worldwide and is transmitted through the fecal-oral route. However, HAV can also be transmitted by blood-derived products. This is due to the fact that viremia occurs during the asymptomatic phase of HAV infection, enabling infected blood or plasma donations to occur. Viral inactivation/removal steps are included during manufacturing of plasmaderived products. However, HAV is a small non-enveloped virus very difficult to remove with traditional viral inactivation procedures. (Source: Transfusion and Apheresis Science)
Source: Transfusion and Apheresis Science - January 12, 2024 Category: Hematology Authors: Luciana Sampieri, Gonzalo Rodr íguez-Lombardi, María Eugenia Bernardi Source Type: research

Red Ribbon Club Inculcation of Positive Deviance Approach to Promote Blood Donation among Undergraduate Medical College Students
Blood is an essential part of human life and blood donation has become a necessity that every society must take into consideration. Health care professionals have a major responsibility in raising community awareness on blood donation. (Source: Transfusion and Apheresis Science)
Source: Transfusion and Apheresis Science - January 11, 2024 Category: Hematology Authors: Reenaa Mohan, M Arulmozhi, R Sindhuri, Pravin Surendran, J Jenifer Florence Mary, Kalaiselvan Ganapathy Source Type: research

Risk of new alloimmunization in patients on anti-CD38 treatment using tube LISS-IAT method
Daratumumab is a monoclonal antibody that targets CD38, a transmembrane protein expressed on many cells including RBCs and to a greater extent on myeloma cells. It has been used for treatment of multiple myeloma and autoimmune diseases. Transfusion management of patients on such therapy can be challenging as these drugs cross-react with RBC surface antigens and cause panreactivity. Material and methods: A retrospective study of the 68 patients treated with anti-CD38 from 2018-2023 was carried out. (Source: Transfusion and Apheresis Science)
Source: Transfusion and Apheresis Science - January 11, 2024 Category: Hematology Authors: Safi ć Stanić Hana, Kruhonja Galić Zrinka, Lukić Martina, Bingulac-Popović Jasna, Jukić Irena Source Type: research

Current principles for the establishment of the virological “cutoff points” in transfusion science and medicine: A short concise overview
Establishing the virological cutoff point, during epidemics, such as the COVID-19 Pandemic, is crucially important as inadequate standardization and the false qualitative results, have enormous clinical and economic consequences when screening for SARS-CoV-2. Moreover, a cutoff point is a crucial step in ensuring a minimal risk of clinical misclassification. Therefore complying with such statutory requirements is an essential part of obligatory good laboratory and clinical practices. In this concise report, we propose a novel methodology for establishing cutoff points for all samples exhibiting detectable levels of the mea...
Source: Transfusion and Apheresis Science - January 11, 2024 Category: Hematology Authors: Paulo Pereira Source Type: research

A targeted survey on teachers ’ perception of training/education for blood donation in high school students: An invited case report for enhancing and retention of dedicated donors in Portugal
Promoting blood donation requires understanding and identifying the factors that motivate donations so that strategies for retaining and increasing loyalty can be developed. Transfusion literacy can be improved through school-based teaching and information about giving and solidarity to promote the development of future donors. For the purpose of aligning any strategies and motivational dynamics that promote adherence to informed donation and loyalty among students in the municipality of Coimbra. (Source: Transfusion and Apheresis Science)
Source: Transfusion and Apheresis Science - January 11, 2024 Category: Hematology Authors: L ídia Matos, Irene Morgado, Luís Santos, Rui Camisa, Paulo Benvindo, Paulo Pereira Source Type: research

Insights on trends in the management of metrological analysis of devices in blood and transfusion medicine services: verification based on their fitness for purpose and suitability for their respective applications
In the field of blood and transfusion medicine, European Directorate for the Quality of Medicines& HealthCare recommends practices for metrological devices. Monitoring and measurement resources, as well as their permitted metrological limits, must be considered in this regard. We must understand variables such as metrological domains, the International System of Units, metrological traceability, and metrological requirements and controls in the field of transfusions in order to understand these metrological devices. (Source: Transfusion and Apheresis Science)
Source: Transfusion and Apheresis Science - January 10, 2024 Category: Hematology Authors: Helga Gon çalves, Carla Rodrigues, Hugo F. Silva, Paulo Pereira Source Type: research

Treatment with plasma exchange of a pregnant woman with anti-pp1pk alloimmunization: a case report
The histo-blood group antigens P, P1 and Pk are a closely related set of glycosphingolipid structures expressed by red blood cells and other tissues. None of these three characters is expressed on p cells, a null phenotype that arises in the context of homozygous mutation of the A4GALT gene. Subjects with p phenotype spontaneously develop a natural alloantibody named anti-PP1Pk, which is a mixture of IgG and IgM against P1, P and Pk. While anti-P1 is a weak cold antibody with poor clinical significance, anti-P and anti-Pk antibodies are potent haemolysins responsible for severe haemolytic transfusion reactions. (Source: Tr...
Source: Transfusion and Apheresis Science - January 9, 2024 Category: Hematology Authors: O. Pignalosa, E. Vigorita, M. Capuano, S. Caruso, A. Mastroianni, S. De Martino, G. Vaccaro, D. Meomartini, C. Nocera Source Type: research

Successful management of severe Kell alloimmunization in pregnancy with intravenous immune globulin
Hemolytic Disease of the Fetus and Newborn (HDFN) is a condition that affects 1 to 2 out of 1000 patients during pregnancy (1). When an alloantibody is present, it is essential to identify its nature in order to organize appropriate follow-up. Kell-mediated HDFN is rare; it occurs in about 5% of Kell alloimmunized pregnant women. It is important to note that in case of anti-Kell immunization, the severity of HDFN is not correlated with maternal antibody titers, and anemia tends to occur earlier and more severely. (Source: Transfusion and Apheresis Science)
Source: Transfusion and Apheresis Science - January 5, 2024 Category: Hematology Authors: Marie Patris, Anne Holoye, Deborah Goldman, Caroline De Coninck, Martin Colard Tags: Case Report Source Type: research

Anemia in Patients undergoing Chronic Therapeutic Plasma Exchange
Therapeutic plasma exchange is known to be an extracorporeal treatment procedure with less adverse effects. Anemia in chronically exchanged patients is not a well-recognized adverse effect. Our aim is to find if adult patients develop anemia while undergoing prolonged TPE treatments and to determine the time of onset of anemia. We retrospectively reviewed all outpatients that have undergone TPE at least once a week from July 2017 to March 2020. Kaplan-Meier time-to-event analysis was employed to calculate the time taken for development of anemia and time to reduction of hemoglobin by 1g/dL from baseline in uncensored patie...
Source: Transfusion and Apheresis Science - December 28, 2023 Category: Hematology Authors: Nichole LePage, Esther Soundar Source Type: research

The Polish adaptation of the Blood Donation Fears Inventory
Given the decline in the number of voluntary blood donors in Poland and the lack of psychological research on blood donors and the donation system in the country, there is a need to develop a tool to measure barriers to recruiting new donors and retaining active donors. One significant barrier is the presence of specific fears associated with the blood donation process. Therefore, the main objective of this study was to perform the Polish adaptation of the Blood Donation Fears Inventory (the BDFI). (Source: Transfusion and Apheresis Science)
Source: Transfusion and Apheresis Science - December 27, 2023 Category: Hematology Authors: Pawe ł Kosowski Source Type: research

In vitro differentiation of human CD45+ CD34+ induced hematopoietic progenitor cells from iPSCs using a monolayer system
Hematopoietic stem cells (HSCs) represent crucial target cells in the management of hematopoietic and immune system disorders. Unfortunately, the primary source of hematopoietic stem cells is limited. Hematopoietic stem cells derived from induced pluripotent stem cells (iPSCs) hold great promise for applications in cell therapy, disease modeling, and drug screening. To achieve a consistent induction method, one specific induction scheme capable of reliably generating CD34 and CD45 double-positive cells from iPSCs was optimized, employing a comparative analysis and screening of various induction methods. (Source: Transfusio...
Source: Transfusion and Apheresis Science - December 20, 2023 Category: Hematology Authors: Zhongfeng Liu, Weihua Liu, Zhiguo Chen, Hua Kang Source Type: research

A study on diagnostic performance of different immunohematological diagnostic tests in assessing the prevalence of ABO Hemolytic Disease of Newborn in the antenatal O group mothers and their neonatal outcome in a tertiary care hospital in Northern India
This study was done to assess the prevalence of ABO-HDN and to compare different immunohematological tests. Methods-In this study 213O group mothers and the 122 ABO-incompatible newborns born to them were included. Quantifying the maternal IgG anti-A/anti-B antibody titer was done by Conventional Tube Technique (CTT) using Dithiothreitol (DTT) pretreated maternal serum. (Source: Transfusion and Apheresis Science)
Source: Transfusion and Apheresis Science - December 12, 2023 Category: Hematology Authors: Joyisa Deb, Ashish Jain, Daljit Kaur, Anupama Bahadur, Sriparna Basu, Gita Negi Source Type: research

I-JAMM-(I): A survey providing an insight into the practices of isoagglutinin titration in ABO incompatible kidney and liver transplantation
ABO-incompatible transplantations allow patients to receive timely transplants. Isoagglutinin titration to ascertain levels of incompatible antibodies in the recipient is important in determining patient selection and transplant survivability. To find out the prevalent trends in India, the largest, first of its kind survey was carried out among the transplant centers regarding their practices in isoagglutinin titration. (Source: Transfusion and Apheresis Science)
Source: Transfusion and Apheresis Science - December 4, 2023 Category: Hematology Authors: Prashant Pandey, Aseem Tiwari, Sudipta Shekhar Das, Shamee Shastry, Vivek Kute, Mohit Chowdhry, Arghyadeep Marik, Geet Aggarwal, Supriya Kumari, Divya Setya, Saikat Mandal, Shweta Ranjan Source Type: research