A guide to ERK dynamics, part 1: mechanisms and models
Biochem J. 2023 Dec 13;480(23):1887-1907. doi: 10.1042/BCJ20230276.ABSTRACTExtracellular signal-regulated kinase (ERK) has long been studied as a key driver of both essential cellular processes and disease. A persistent question has been how this single pathway is able to direct multiple cell behaviors, including growth, proliferation, and death. Modern biosensor studies have revealed that the temporal pattern of ERK activity is highly variable and heterogeneous, and critically, that these dynamic differences modulate cell fate. This two-part review discusses the current understanding of dynamic activity in the ERK pathway...
Source: The Biochemical Journal - December 1, 2023 Category: Biochemistry Authors: Abhineet Ram Devan Murphy Nicholaus DeCuzzi Madhura Patankar Jason Hu Michael Pargett John G Albeck Source Type: research
A guide to ERK dynamics, part 2: downstream decoding
Biochem J. 2023 Dec 13;480(23):1909-1928. doi: 10.1042/BCJ20230277.ABSTRACTSignaling by the extracellular signal-regulated kinase (ERK) pathway controls many cellular processes, including cell division, death, and differentiation. In this second installment of a two-part review, we address the question of how the ERK pathway exerts distinct and context-specific effects on multiple processes. We discuss how the dynamics of ERK activity induce selective changes in gene expression programs, with insights from both experiments and computational models. With a focus on single-cell biosensor-based studies, we summarize four majo...
Source: The Biochemical Journal - December 1, 2023 Category: Biochemistry Authors: Abhineet Ram Devan Murphy Nicholaus DeCuzzi Madhura Patankar Jason Hu Michael Pargett John G Albeck Source Type: research
Skin in the game: pannexin channels in healthy and cancerous skin
Biochem J. 2023 Dec 13;480(23):1929-1949. doi: 10.1042/BCJ20230176.ABSTRACTThe skin is a highly organized tissue composed of multiple layers and cell types that require coordinated cell to cell communication to maintain tissue homeostasis. In skin cancer, this organized structure and communication is disrupted, prompting the malignant transformation of healthy cells into melanoma, basal cell carcinoma or squamous cell carcinoma tumours. One such family of channel proteins critical for cellular communication is pannexins (PANX1, PANX2, PANX3), all of which are present in the skin. These heptameric single-membrane channels a...
Source: The Biochemical Journal - December 1, 2023 Category: Biochemistry Authors: Brooke L O'Donnell Silvia Penuela Source Type: research
A guide to ERK dynamics, part 1: mechanisms and models
Biochem J. 2023 Dec 13;480(23):1887-1907. doi: 10.1042/BCJ20230276.ABSTRACTExtracellular signal-regulated kinase (ERK) has long been studied as a key driver of both essential cellular processes and disease. A persistent question has been how this single pathway is able to direct multiple cell behaviors, including growth, proliferation, and death. Modern biosensor studies have revealed that the temporal pattern of ERK activity is highly variable and heterogeneous, and critically, that these dynamic differences modulate cell fate. This two-part review discusses the current understanding of dynamic activity in the ERK pathway...
Source: The Biochemical Journal - December 1, 2023 Category: Biochemistry Authors: Abhineet Ram Devan Murphy Nicholaus DeCuzzi Madhura Patankar Jason Hu Michael Pargett John G Albeck Source Type: research
A guide to ERK dynamics, part 2: downstream decoding
Biochem J. 2023 Dec 13;480(23):1909-1928. doi: 10.1042/BCJ20230277.ABSTRACTSignaling by the extracellular signal-regulated kinase (ERK) pathway controls many cellular processes, including cell division, death, and differentiation. In this second installment of a two-part review, we address the question of how the ERK pathway exerts distinct and context-specific effects on multiple processes. We discuss how the dynamics of ERK activity induce selective changes in gene expression programs, with insights from both experiments and computational models. With a focus on single-cell biosensor-based studies, we summarize four majo...
Source: The Biochemical Journal - December 1, 2023 Category: Biochemistry Authors: Abhineet Ram Devan Murphy Nicholaus DeCuzzi Madhura Patankar Jason Hu Michael Pargett John G Albeck Source Type: research
Skin in the game: pannexin channels in healthy and cancerous skin
Biochem J. 2023 Dec 13;480(23):1929-1949. doi: 10.1042/BCJ20230176.ABSTRACTThe skin is a highly organized tissue composed of multiple layers and cell types that require coordinated cell to cell communication to maintain tissue homeostasis. In skin cancer, this organized structure and communication is disrupted, prompting the malignant transformation of healthy cells into melanoma, basal cell carcinoma or squamous cell carcinoma tumours. One such family of channel proteins critical for cellular communication is pannexins (PANX1, PANX2, PANX3), all of which are present in the skin. These heptameric single-membrane channels a...
Source: The Biochemical Journal - December 1, 2023 Category: Biochemistry Authors: Brooke L O'Donnell Silvia Penuela Source Type: research
A guide to ERK dynamics, part 1: mechanisms and models
Biochem J. 2023 Dec 13;480(23):1887-1907. doi: 10.1042/BCJ20230276.ABSTRACTExtracellular signal-regulated kinase (ERK) has long been studied as a key driver of both essential cellular processes and disease. A persistent question has been how this single pathway is able to direct multiple cell behaviors, including growth, proliferation, and death. Modern biosensor studies have revealed that the temporal pattern of ERK activity is highly variable and heterogeneous, and critically, that these dynamic differences modulate cell fate. This two-part review discusses the current understanding of dynamic activity in the ERK pathway...
Source: The Biochemical Journal - December 1, 2023 Category: Biochemistry Authors: Abhineet Ram Devan Murphy Nicholaus DeCuzzi Madhura Patankar Jason Hu Michael Pargett John G Albeck Source Type: research
A guide to ERK dynamics, part 2: downstream decoding
Biochem J. 2023 Dec 13;480(23):1909-1928. doi: 10.1042/BCJ20230277.ABSTRACTSignaling by the extracellular signal-regulated kinase (ERK) pathway controls many cellular processes, including cell division, death, and differentiation. In this second installment of a two-part review, we address the question of how the ERK pathway exerts distinct and context-specific effects on multiple processes. We discuss how the dynamics of ERK activity induce selective changes in gene expression programs, with insights from both experiments and computational models. With a focus on single-cell biosensor-based studies, we summarize four majo...
Source: The Biochemical Journal - December 1, 2023 Category: Biochemistry Authors: Abhineet Ram Devan Murphy Nicholaus DeCuzzi Madhura Patankar Jason Hu Michael Pargett John G Albeck Source Type: research
The role of phosphorylation in calmodulin-mediated gating of human AQP0
Biochem J. 2023 Nov 30:BCJ20230158. doi: 10.1042/BCJ20230158. Online ahead of print.ABSTRACTAquaporin-0 (AQP0) is the main water channel in the mammalian lens and is involved in accommodation and maintaining lens transparency. AQP0 binds the Ca2+-sensing protein calmodulin (CaM) and this interaction is believed to gate its water permeability by closing the water-conducting pore. Here we express recombinant and functional human AQP0 in P. pastoris and investigate how phosphorylation affects the interaction with CaM in vitro as well as the CaM-dependent water permeability of AQP0 in proteoliposomes. Using microscale thermoph...
Source: The Biochemical Journal - November 30, 2023 Category: Biochemistry Authors: Stefan Kreida Jennifer Virginia Roche Julie Winkel Missel Tamim Al-Jubair Carl Johan Hagstr ömer Veronika Wittenbecher Sara Linse Pontus Gourdon Susanna T örnroth-Horsefield Source Type: research
The role of phosphorylation in calmodulin-mediated gating of human AQP0
Biochem J. 2023 Nov 30:BCJ20230158. doi: 10.1042/BCJ20230158. Online ahead of print.ABSTRACTAquaporin-0 (AQP0) is the main water channel in the mammalian lens and is involved in accommodation and maintaining lens transparency. AQP0 binds the Ca2+-sensing protein calmodulin (CaM) and this interaction is believed to gate its water permeability by closing the water-conducting pore. Here we express recombinant and functional human AQP0 in P. pastoris and investigate how phosphorylation affects the interaction with CaM in vitro as well as the CaM-dependent water permeability of AQP0 in proteoliposomes. Using microscale thermoph...
Source: The Biochemical Journal - November 30, 2023 Category: Biochemistry Authors: Stefan Kreida Jennifer Virginia Roche Julie Winkel Missel Tamim Al-Jubair Carl Johan Hagstr ömer Veronika Wittenbecher Sara Linse Pontus Gourdon Susanna T örnroth-Horsefield Source Type: research
Leukotriene B4 receptor 1 (BLT1) does not mediate disease progression in a mouse model of liver fibrosis
Biochem J. 2023 Nov 28:BCJ20230422. doi: 10.1042/BCJ20230422. Online ahead of print.ABSTRACTMASH is a prevalent liver disease that can progress to fibrosis, cirrhosis, hepatocellular carcinoma (HCC), and ultimately death, but there are no approved therapies. Leukotriene B4 (LTB4) is a potent pro-inflammatory chemoattractant that drives macrophage and neutrophil chemotaxis, and genetic loss or inhibition of its high affinity receptor, leukotriene B4 receptor 1 (BLT1), results in improved insulin sensitivity and decreased hepatic steatosis. To validate the therapeutic efficacy of BLT1 inhibition in an inflammatory and pro-fi...
Source: The Biochemical Journal - November 28, 2023 Category: Biochemistry Authors: Erin Coyne Yilin Nie Desiree Abdurrachim Charlene Lin Zhi Ong Yongqi Zhou Asad Abu Bakar Ali Stacey Meyers Jeff Grein Wendy Blumenschein Brendan Gongol Yang Liu Cedric Lorenz Hugelshofer Ester Carballo-Jane Saswata Talukdar Source Type: research
Sperm induce a secondary increase in ATP levels in mouse eggs that is independent of Ca2+ oscillations
Biochem J. 2023 Nov 28:BCJ20230065. doi: 10.1042/BCJ20230065. Online ahead of print.ABSTRACTEgg activation at fertilization in mouse eggs is caused by a series of cytosolic Ca2+ oscillations that are associated with an increase in ATP concentrations driven by increased mitochondrial activity. We have investigated the role of Ca2+ oscillations in these changes in ATP at fertilization by measuring the dynamics of ATP and Ca2+ in mouse eggs. An initial ATP increase started with the first Ca2+ transient at fertilization and then a secondary increase in ATP occurred about 1 hour later and this preceded a small and temporary inc...
Source: The Biochemical Journal - November 28, 2023 Category: Biochemistry Authors: Cindy Ikie-Eshalomi Elnur Aliyev Sven Hoehn Thomasz Jukowski Karl Swann Source Type: research
Leukotriene B4 receptor 1 (BLT1) does not mediate disease progression in a mouse model of liver fibrosis
Biochem J. 2023 Nov 28:BCJ20230422. doi: 10.1042/BCJ20230422. Online ahead of print.ABSTRACTMASH is a prevalent liver disease that can progress to fibrosis, cirrhosis, hepatocellular carcinoma (HCC), and ultimately death, but there are no approved therapies. Leukotriene B4 (LTB4) is a potent pro-inflammatory chemoattractant that drives macrophage and neutrophil chemotaxis, and genetic loss or inhibition of its high affinity receptor, leukotriene B4 receptor 1 (BLT1), results in improved insulin sensitivity and decreased hepatic steatosis. To validate the therapeutic efficacy of BLT1 inhibition in an inflammatory and pro-fi...
Source: The Biochemical Journal - November 28, 2023 Category: Biochemistry Authors: Erin Coyne Yilin Nie Desiree Abdurrachim Charlene Lin Zhi Ong Yongqi Zhou Asad Abu Bakar Ali Stacey Meyers Jeff Grein Wendy Blumenschein Brendan Gongol Yang Liu Cedric Lorenz Hugelshofer Ester Carballo-Jane Saswata Talukdar Source Type: research
Sperm induce a secondary increase in ATP levels in mouse eggs that is independent of Ca2+ oscillations
Biochem J. 2023 Nov 28:BCJ20230065. doi: 10.1042/BCJ20230065. Online ahead of print.ABSTRACTEgg activation at fertilization in mouse eggs is caused by a series of cytosolic Ca2+ oscillations that are associated with an increase in ATP concentrations driven by increased mitochondrial activity. We have investigated the role of Ca2+ oscillations in these changes in ATP at fertilization by measuring the dynamics of ATP and Ca2+ in mouse eggs. An initial ATP increase started with the first Ca2+ transient at fertilization and then a secondary increase in ATP occurred about 1 hour later and this preceded a small and temporary inc...
Source: The Biochemical Journal - November 28, 2023 Category: Biochemistry Authors: Cindy Ikie-Eshalomi Elnur Aliyev Sven Hoehn Thomasz Jukowski Karl Swann Source Type: research
Leukotriene B4 receptor 1 (BLT1) does not mediate disease progression in a mouse model of liver fibrosis
Biochem J. 2023 Nov 28:BCJ20230422. doi: 10.1042/BCJ20230422. Online ahead of print.ABSTRACTMASH is a prevalent liver disease that can progress to fibrosis, cirrhosis, hepatocellular carcinoma (HCC), and ultimately death, but there are no approved therapies. Leukotriene B4 (LTB4) is a potent pro-inflammatory chemoattractant that drives macrophage and neutrophil chemotaxis, and genetic loss or inhibition of its high affinity receptor, leukotriene B4 receptor 1 (BLT1), results in improved insulin sensitivity and decreased hepatic steatosis. To validate the therapeutic efficacy of BLT1 inhibition in an inflammatory and pro-fi...
Source: The Biochemical Journal - November 28, 2023 Category: Biochemistry Authors: Erin Coyne Yilin Nie Desiree Abdurrachim Charlene Lin Zhi Ong Yongqi Zhou Asad Abu Bakar Ali Stacey Meyers Jeff Grein Wendy Blumenschein Brendan Gongol Yang Liu Cedric Lorenz Hugelshofer Ester Carballo-Jane Saswata Talukdar Source Type: research
