MicroRNA, miR-501 Regulate the V(D)J Recombination in B Cells
Biochem J. 2023 Dec 12:BCJ20230250. doi: 10.1042/BCJ20230250. Online ahead of print.ABSTRACTThe stringent regulation of RAGs (Recombination activating genes), the site-specific endonuclease responsible for V(D)J recombination, is important to prevent genomic rearrangements and chromosomal translocations in lymphoid cells. In the present study, we identify a microRNA, miR-501, which can regulate the expression of RAG1 in lymphoid cells. Overexpression of the pre-miRNA construct led to the generation of mature miRNAs and a concomitant reduction of RAG1 expression, whereas inhibition using anti-miRs resulted in its enhanced e...
Source: The Biochemical Journal - December 12, 2023 Category: Biochemistry Authors: Rupa Kumari Urbi Roy Sagar Desai Arannya Mondal Rajshree R Nair Namrata Nilavar Bibha Choudhary Sathees C Raghavan Source Type: research
MicroRNA, miR-501 Regulate the V(D)J Recombination in B Cells
Biochem J. 2023 Dec 12:BCJ20230250. doi: 10.1042/BCJ20230250. Online ahead of print.ABSTRACTThe stringent regulation of RAGs (Recombination activating genes), the site-specific endonuclease responsible for V(D)J recombination, is important to prevent genomic rearrangements and chromosomal translocations in lymphoid cells. In the present study, we identify a microRNA, miR-501, which can regulate the expression of RAG1 in lymphoid cells. Overexpression of the pre-miRNA construct led to the generation of mature miRNAs and a concomitant reduction of RAG1 expression, whereas inhibition using anti-miRs resulted in its enhanced e...
Source: The Biochemical Journal - December 12, 2023 Category: Biochemistry Authors: Rupa Kumari Urbi Roy Sagar Desai Arannya Mondal Rajshree R Nair Namrata Nilavar Bibha Choudhary Sathees C Raghavan Source Type: research
MicroRNA, miR-501 Regulate the V(D)J Recombination in B Cells
Biochem J. 2023 Dec 12:BCJ20230250. doi: 10.1042/BCJ20230250. Online ahead of print.ABSTRACTThe stringent regulation of RAGs (Recombination activating genes), the site-specific endonuclease responsible for V(D)J recombination, is important to prevent genomic rearrangements and chromosomal translocations in lymphoid cells. In the present study, we identify a microRNA, miR-501, which can regulate the expression of RAG1 in lymphoid cells. Overexpression of the pre-miRNA construct led to the generation of mature miRNAs and a concomitant reduction of RAG1 expression, whereas inhibition using anti-miRs resulted in its enhanced e...
Source: The Biochemical Journal - December 12, 2023 Category: Biochemistry Authors: Rupa Kumari Urbi Roy Sagar Desai Arannya Mondal Rajshree R Nair Namrata Nilavar Bibha Choudhary Sathees C Raghavan Source Type: research
Upregulation of SLC7A11/xCT creates a vulnerability to selenocystine-induced cytotoxicity
Biochem J. 2023 Dec 11:BCJ20230317. doi: 10.1042/BCJ20230317. Online ahead of print.ABSTRACTThe SLC7A11/xCT cystine and glutamate antiporter has emerged as an attractive target for cancer therapy due to its selective overexpression in multiple cancers and its role in preventing ferroptosis. Utilizing pharmacological and genetic approaches in hepatocellular carcinoma cell lines, we demonstrate that overexpression of SLC7A11 engenders hypersensitivity towards L-selenocystine, a naturally-occurring diselenide that bears close structural similarity to L-cystine. We find that abundance of SLC7A11 positively correlates with sens...
Source: The Biochemical Journal - December 11, 2023 Category: Biochemistry Authors: Shawn Lu Wen Tan Hui Min Tan Erez Israeli Indah Fatihah Vignesh Ramachandran Shamsia Bte Ali Shane Jun An Goh Jillian Wee Alicia Qian Ler Tan Wai Leong Tam Weiping Han Source Type: research
Engineering of CYP82Y1, a cytochrome P450 monooxygenase: a key enzyme in noscapine biosynthesis in opium poppy
Biochem J. 2023 Dec 13;480(23):2009-2022. doi: 10.1042/BCJ20230243.ABSTRACTProtein engineering provides a powerful base for the circumvention of challenges tied with characteristics accountable for enzyme functions. CYP82Y1 introduces a hydroxyl group (-OH) into C1 of N-methylcanadine as the substrate to yield 1-hydroxy-N-methylcanadine. This chemical process has been found to be the gateway to noscapine biosynthesis. Owning to the importance of CYP82Y1 in this biosynthetic pathway, it has been selected as a target for enzyme engineering. The insertion of tags to the N- and C-terminal of CYP82Y1 was assessed for their effi...
Source: The Biochemical Journal - December 8, 2023 Category: Biochemistry Authors: Zahra Aghaali Mohammad Reza Naghavi Source Type: research
Engineering of CYP82Y1, a cytochrome P450 monooxygenase: a key enzyme in noscapine biosynthesis in opium poppy
Biochem J. 2023 Dec 13;480(23):2009-2022. doi: 10.1042/BCJ20230243.ABSTRACTProtein engineering provides a powerful base for the circumvention of challenges tied with characteristics accountable for enzyme functions. CYP82Y1 introduces a hydroxyl group (-OH) into C1 of N-methylcanadine as the substrate to yield 1-hydroxy-N-methylcanadine. This chemical process has been found to be the gateway to noscapine biosynthesis. Owning to the importance of CYP82Y1 in this biosynthetic pathway, it has been selected as a target for enzyme engineering. The insertion of tags to the N- and C-terminal of CYP82Y1 was assessed for their effi...
Source: The Biochemical Journal - December 8, 2023 Category: Biochemistry Authors: Zahra Aghaali Mohammad Reza Naghavi Source Type: research
Engineering of CYP82Y1, a cytochrome P450 monooxygenase: a key enzyme in noscapine biosynthesis in opium poppy
Biochem J. 2023 Dec 13;480(23):2009-2022. doi: 10.1042/BCJ20230243.ABSTRACTProtein engineering provides a powerful base for the circumvention of challenges tied with characteristics accountable for enzyme functions. CYP82Y1 introduces a hydroxyl group (-OH) into C1 of N-methylcanadine as the substrate to yield 1-hydroxy-N-methylcanadine. This chemical process has been found to be the gateway to noscapine biosynthesis. Owning to the importance of CYP82Y1 in this biosynthetic pathway, it has been selected as a target for enzyme engineering. The insertion of tags to the N- and C-terminal of CYP82Y1 was assessed for their effi...
Source: The Biochemical Journal - December 8, 2023 Category: Biochemistry Authors: Zahra Aghaali Mohammad Reza Naghavi Source Type: research
A highly conserved ligand-binding site for AccA transporters of antibiotic and quorum-sensing regulator in Agrobacterium leads to a different specificity
Biochem J. 2023 Dec 6:BCJ20230273. doi: 10.1042/BCJ20230273. Online ahead of print.ABSTRACTPlants genetically modified by the pathogenic Agrobacterium strain C58 synthesize agrocinopines A and B, whereas those modified by the pathogenic strain Bo542 produce agrocinopines C and D. The four agrocinopines (A, B, C and D) serve as nutrients by agrobacteria and signaling molecule for the dissemination of virulence genes. They share the uncommon pyranose-2-phosphate motif, represented by the L-arabinopyranose moiety in agrocinopines A/B and the D-glucopyranose moiety in agrocinopines C/D, also found in the antibiotic agrocin 84....
Source: The Biochemical Journal - December 7, 2023 Category: Biochemistry Authors: Solange Mor éra Armelle Vigouroux Magali Aumont-Nicaise Mohammed Ahmar Thibault Meyer Abbas El Sahili Gr égory Deicsics Almudena Gonz ález-Mula Sizhe Li Jeanne Dor é Serena Siragu Pierre Legrand Camille Penot Fran çois Andrè Denis Faure Laurent Soul Source Type: research
A highly conserved ligand-binding site for AccA transporters of antibiotic and quorum-sensing regulator in Agrobacterium leads to a different specificity
Biochem J. 2023 Dec 6:BCJ20230273. doi: 10.1042/BCJ20230273. Online ahead of print.ABSTRACTPlants genetically modified by the pathogenic Agrobacterium strain C58 synthesize agrocinopines A and B, whereas those modified by the pathogenic strain Bo542 produce agrocinopines C and D. The four agrocinopines (A, B, C and D) serve as nutrients by agrobacteria and signaling molecule for the dissemination of virulence genes. They share the uncommon pyranose-2-phosphate motif, represented by the L-arabinopyranose moiety in agrocinopines A/B and the D-glucopyranose moiety in agrocinopines C/D, also found in the antibiotic agrocin 84....
Source: The Biochemical Journal - December 7, 2023 Category: Biochemistry Authors: Solange Mor éra Armelle Vigouroux Magali Aumont-Nicaise Mohammed Ahmar Thibault Meyer Abbas El Sahili Gr égory Deicsics Almudena Gonz ález-Mula Sizhe Li Jeanne Dor é Serena Siragu Pierre Legrand Camille Penot Fran çois Andrè Denis Faure Laurent Soul Source Type: research
Understanding interleukin 11 as a disease gene and therapeutic target
Biochem J. 2023 Dec 13;480(23):1987-2008. doi: 10.1042/BCJ20220160.ABSTRACTInterleukin 11 (IL11) is an elusive member of the IL6 family of cytokines. While initially thought to be a haematopoietic and cytoprotective factor, more recent data show instead that IL11 is redundant for haematopoiesis and toxic. In this review, the reasons that led to the original misunderstandings of IL11 biology, which are now understandable, are explained with particular attention on the use of recombinant human IL11 in mice and humans. Following tissue injury, as part of an evolutionary ancient homeostatic response, IL11 is secreted from dama...
Source: The Biochemical Journal - December 6, 2023 Category: Biochemistry Authors: Stuart A Cook Source Type: research
Lysine methylation signaling in skeletal muscle biology: from myogenesis to clinical insights
Biochem J. 2023 Dec 13;480(23):1969-1986. doi: 10.1042/BCJ20230223.ABSTRACTLysine methylation signaling is well studied for its key roles in the regulation of transcription states through modifications on histone proteins. While histone lysine methylation has been extensively studied, recent discoveries of lysine methylation on thousands of non-histone proteins has broadened our appreciation for this small chemical modification in the regulation of protein function. In this review, we highlight the significance of histone and non-histone lysine methylation signaling in skeletal muscle biology, spanning development, mainten...
Source: The Biochemical Journal - December 6, 2023 Category: Biochemistry Authors: Eric Cordeiro-Spinetti Scott B Rothbart Source Type: research
Understanding interleukin 11 as a disease gene and therapeutic target
Biochem J. 2023 Dec 13;480(23):1987-2008. doi: 10.1042/BCJ20220160.ABSTRACTInterleukin 11 (IL11) is an elusive member of the IL6 family of cytokines. While initially thought to be a haematopoietic and cytoprotective factor, more recent data show instead that IL11 is redundant for haematopoiesis and toxic. In this review, the reasons that led to the original misunderstandings of IL11 biology, which are now understandable, are explained with particular attention on the use of recombinant human IL11 in mice and humans. Following tissue injury, as part of an evolutionary ancient homeostatic response, IL11 is secreted from dama...
Source: The Biochemical Journal - December 6, 2023 Category: Biochemistry Authors: Stuart A Cook Source Type: research
Lysine methylation signaling in skeletal muscle biology: from myogenesis to clinical insights
Biochem J. 2023 Dec 13;480(23):1969-1986. doi: 10.1042/BCJ20230223.ABSTRACTLysine methylation signaling is well studied for its key roles in the regulation of transcription states through modifications on histone proteins. While histone lysine methylation has been extensively studied, recent discoveries of lysine methylation on thousands of non-histone proteins has broadened our appreciation for this small chemical modification in the regulation of protein function. In this review, we highlight the significance of histone and non-histone lysine methylation signaling in skeletal muscle biology, spanning development, mainten...
Source: The Biochemical Journal - December 6, 2023 Category: Biochemistry Authors: Eric Cordeiro-Spinetti Scott B Rothbart Source Type: research
Skin in the game: pannexin channels in healthy and cancerous skin
Biochem J. 2023 Dec 13;480(23):1929-1949. doi: 10.1042/BCJ20230176.ABSTRACTThe skin is a highly organized tissue composed of multiple layers and cell types that require coordinated cell to cell communication to maintain tissue homeostasis. In skin cancer, this organized structure and communication is disrupted, prompting the malignant transformation of healthy cells into melanoma, basal cell carcinoma or squamous cell carcinoma tumours. One such family of channel proteins critical for cellular communication is pannexins (PANX1, PANX2, PANX3), all of which are present in the skin. These heptameric single-membrane channels a...
Source: The Biochemical Journal - December 1, 2023 Category: Biochemistry Authors: Brooke L O'Donnell Silvia Penuela Source Type: research
A guide to ERK dynamics, part 1: mechanisms and models
Biochem J. 2023 Dec 13;480(23):1887-1907. doi: 10.1042/BCJ20230276.ABSTRACTExtracellular signal-regulated kinase (ERK) has long been studied as a key driver of both essential cellular processes and disease. A persistent question has been how this single pathway is able to direct multiple cell behaviors, including growth, proliferation, and death. Modern biosensor studies have revealed that the temporal pattern of ERK activity is highly variable and heterogeneous, and critically, that these dynamic differences modulate cell fate. This two-part review discusses the current understanding of dynamic activity in the ERK pathway...
Source: The Biochemical Journal - December 1, 2023 Category: Biochemistry Authors: Abhineet Ram Devan Murphy Nicholaus DeCuzzi Madhura Patankar Jason Hu Michael Pargett John G Albeck Source Type: research
