In search for structural targets for engineering D-amino acid transaminase: modulation of pH optimum and substrate specificity
Biochem J. 2023 Aug 7:BCJ20230233. doi: 10.1042/BCJ20230233. Online ahead of print.ABSTRACTThe development of biocatalysts requires reorganization of the enzyme's active site to facilitate the productive binding of the target substrate and improve turnover number at desired conditions. Pyridoxal-5'-phosphate (PLP)-dependent transaminases are highly efficient biocatalysts for asymmetric amination of ketones and keto acids. However, transaminases, being stereoselective enzymes, have a narrow substrate specificity due to the ordered structure of the active site and work only in neutral-alkaline media. Here, we investigated th...
Source: The Biochemical Journal - August 7, 2023 Category: Biochemistry Authors: Sofia A Shilova Ilya O Matuta Maria G Khrenova Alena Y Nikolaeva Natalia L Klyachko Mikhail E Minyaev Alex R Khomutov Konstantin M Boyko Vladimir O Popov Ekaterina Yu Bezsudnova Source Type: research
In search for structural targets for engineering D-amino acid transaminase: modulation of pH optimum and substrate specificity
Biochem J. 2023 Aug 7:BCJ20230233. doi: 10.1042/BCJ20230233. Online ahead of print.ABSTRACTThe development of biocatalysts requires reorganization of the enzyme's active site to facilitate the productive binding of the target substrate and improve turnover number at desired conditions. Pyridoxal-5'-phosphate (PLP)-dependent transaminases are highly efficient biocatalysts for asymmetric amination of ketones and keto acids. However, transaminases, being stereoselective enzymes, have a narrow substrate specificity due to the ordered structure of the active site and work only in neutral-alkaline media. Here, we investigated th...
Source: The Biochemical Journal - August 7, 2023 Category: Biochemistry Authors: Sofia A Shilova Ilya O Matuta Maria G Khrenova Alena Y Nikolaeva Natalia L Klyachko Mikhail E Minyaev Alex R Khomutov Konstantin M Boyko Vladimir O Popov Ekaterina Yu Bezsudnova Source Type: research
Elevated CO2 alters photosynthesis, growth and susceptibility to powdery mildew of oak seedling
Biochem J. 2023 Jul 27:BCJ20230002. doi: 10.1042/BCJ20230002. Online ahead of print.ABSTRACTElevated CO2 (eCO2) is a determinant factor of climate change and is known to alter plant processes such as physiology, growth and resistance to pathogens. Quercus robur, a tree species integrated in most forest regeneration strategies, shows high vulnerability to powdery mildew (PM) disease at the seedling stage. PM is present in most oak forests and it is considered a bottleneck for oak woodland regeneration. Our study aims to decipher the effect of eCO2 on plant responses to PM. Oak seedlings were grown in controlled environment ...
Source: The Biochemical Journal - July 27, 2023 Category: Biochemistry Authors: Rosa Sanchez-Lucas Carolina Mayoral Mark Raw Maria-Anna Mousouraki Estrella Luna Source Type: research
Insights into the central role of N-acetyl-glucosamine-1-phosphate uridyltransferase (GlmU) in peptidoglycan metabolism and its potential as a therapeutic target
Biochem J. 2023 Jun 18;480(15):1147-1164. doi: 10.1042/BCJ20230173.ABSTRACTSeveral decades after the discovery of the first antibiotic (penicillin) microbes have evolved novel mechanisms of resistance; endangering not only our abilities to combat future bacterial pandemics but many other clinical challenges such as acquired infections during surgeries. Antimicrobial resistance (AMR) is attributed to the mismanagement and overuse of these medications and is complicated by a slower rate of the discovery of novel drugs and targets. Bacterial peptidoglycan (PG), a three-dimensional mesh of glycan units, is the foundation of th...
Source: The Biochemical Journal - July 27, 2023 Category: Biochemistry Authors: Vijay Soni Eric H Rosenn Ramya Venkataraman Source Type: research
Elevated CO2 alters photosynthesis, growth and susceptibility to powdery mildew of oak seedling
Biochem J. 2023 Jul 27:BCJ20230002. doi: 10.1042/BCJ20230002. Online ahead of print.ABSTRACTElevated CO2 (eCO2) is a determinant factor of climate change and is known to alter plant processes such as physiology, growth and resistance to pathogens. Quercus robur, a tree species integrated in most forest regeneration strategies, shows high vulnerability to powdery mildew (PM) disease at the seedling stage. PM is present in most oak forests and it is considered a bottleneck for oak woodland regeneration. Our study aims to decipher the effect of eCO2 on plant responses to PM. Oak seedlings were grown in controlled environment ...
Source: The Biochemical Journal - July 27, 2023 Category: Biochemistry Authors: Rosa Sanchez-Lucas Carolina Mayoral Mark Raw Maria-Anna Mousouraki Estrella Luna Source Type: research
Insights into the central role of N-acetyl-glucosamine-1-phosphate uridyltransferase (GlmU) in peptidoglycan metabolism and its potential as a therapeutic target
Biochem J. 2023 Jun 18;480(15):1147-1164. doi: 10.1042/BCJ20230173.ABSTRACTSeveral decades after the discovery of the first antibiotic (penicillin) microbes have evolved novel mechanisms of resistance; endangering not only our abilities to combat future bacterial pandemics but many other clinical challenges such as acquired infections during surgeries. Antimicrobial resistance (AMR) is attributed to the mismanagement and overuse of these medications and is complicated by a slower rate of the discovery of novel drugs and targets. Bacterial peptidoglycan (PG), a three-dimensional mesh of glycan units, is the foundation of th...
Source: The Biochemical Journal - July 27, 2023 Category: Biochemistry Authors: Vijay Soni Eric H Rosenn Ramya Venkataraman Source Type: research
Elevated CO2 alters photosynthesis, growth and susceptibility to powdery mildew of oak seedling
Biochem J. 2023 Jul 27:BCJ20230002. doi: 10.1042/BCJ20230002. Online ahead of print.ABSTRACTElevated CO2 (eCO2) is a determinant factor of climate change and is known to alter plant processes such as physiology, growth and resistance to pathogens. Quercus robur, a tree species integrated in most forest regeneration strategies, shows high vulnerability to powdery mildew (PM) disease at the seedling stage. PM is present in most oak forests and it is considered a bottleneck for oak woodland regeneration. Our study aims to decipher the effect of eCO2 on plant responses to PM. Oak seedlings were grown in controlled environment ...
Source: The Biochemical Journal - July 27, 2023 Category: Biochemistry Authors: Rosa Sanchez-Lucas Carolina Mayoral Mark Raw Maria-Anna Mousouraki Estrella Luna Source Type: research
Insights into the central role of N-acetyl-glucosamine-1-phosphate uridyltransferase (GlmU) in peptidoglycan metabolism and its potential as a therapeutic target
Biochem J. 2023 Jun 18;480(15):1147-1164. doi: 10.1042/BCJ20230173.ABSTRACTSeveral decades after the discovery of the first antibiotic (penicillin) microbes have evolved novel mechanisms of resistance; endangering not only our abilities to combat future bacterial pandemics but many other clinical challenges such as acquired infections during surgeries. Antimicrobial resistance (AMR) is attributed to the mismanagement and overuse of these medications and is complicated by a slower rate of the discovery of novel drugs and targets. Bacterial peptidoglycan (PG), a three-dimensional mesh of glycan units, is the foundation of th...
Source: The Biochemical Journal - July 27, 2023 Category: Biochemistry Authors: Vijay Soni Eric H Rosenn Ramya Venkataraman Source Type: research
Elevated CO2 alters photosynthesis, growth and susceptibility to powdery mildew of oak seedling
Biochem J. 2023 Jul 27:BCJ20230002. doi: 10.1042/BCJ20230002. Online ahead of print.ABSTRACTElevated CO2 (eCO2) is a determinant factor of climate change and is known to alter plant processes such as physiology, growth and resistance to pathogens. Quercus robur, a tree species integrated in most forest regeneration strategies, shows high vulnerability to powdery mildew (PM) disease at the seedling stage. PM is present in most oak forests and it is considered a bottleneck for oak woodland regeneration. Our study aims to decipher the effect of eCO2 on plant responses to PM. Oak seedlings were grown in controlled environment ...
Source: The Biochemical Journal - July 27, 2023 Category: Biochemistry Authors: Rosa Sanchez-Lucas Carolina Mayoral Mark Raw Maria-Anna Mousouraki Estrella Luna Source Type: research
Insights into the central role of N-acetyl-glucosamine-1-phosphate uridyltransferase (GlmU) in peptidoglycan metabolism and its potential as a therapeutic target
Biochem J. 2023 Jun 18;480(15):1147-1164. doi: 10.1042/BCJ20230173.ABSTRACTSeveral decades after the discovery of the first antibiotic (penicillin) microbes have evolved novel mechanisms of resistance; endangering not only our abilities to combat future bacterial pandemics but many other clinical challenges such as acquired infections during surgeries. Antimicrobial resistance (AMR) is attributed to the mismanagement and overuse of these medications and is complicated by a slower rate of the discovery of novel drugs and targets. Bacterial peptidoglycan (PG), a three-dimensional mesh of glycan units, is the foundation of th...
Source: The Biochemical Journal - July 27, 2023 Category: Biochemistry Authors: Vijay Soni Eric H Rosenn Ramya Venkataraman Source Type: research
Elevated CO2 alters photosynthesis, growth and susceptibility to powdery mildew of oak seedling
Biochem J. 2023 Jul 27:BCJ20230002. doi: 10.1042/BCJ20230002. Online ahead of print.ABSTRACTElevated CO2 (eCO2) is a determinant factor of climate change and is known to alter plant processes such as physiology, growth and resistance to pathogens. Quercus robur, a tree species integrated in most forest regeneration strategies, shows high vulnerability to powdery mildew (PM) disease at the seedling stage. PM is present in most oak forests and it is considered a bottleneck for oak woodland regeneration. Our study aims to decipher the effect of eCO2 on plant responses to PM. Oak seedlings were grown in controlled environment ...
Source: The Biochemical Journal - July 27, 2023 Category: Biochemistry Authors: Rosa Sanchez-Lucas Carolina Mayoral Mark Raw Maria-Anna Mousouraki Estrella Luna Source Type: research
Insights into the central role of N-acetyl-glucosamine-1-phosphate uridyltransferase (GlmU) in peptidoglycan metabolism and its potential as a therapeutic target
Biochem J. 2023 Jun 18;480(15):1147-1164. doi: 10.1042/BCJ20230173.ABSTRACTSeveral decades after the discovery of the first antibiotic (penicillin) microbes have evolved novel mechanisms of resistance; endangering not only our abilities to combat future bacterial pandemics but many other clinical challenges such as acquired infections during surgeries. Antimicrobial resistance (AMR) is attributed to the mismanagement and overuse of these medications and is complicated by a slower rate of the discovery of novel drugs and targets. Bacterial peptidoglycan (PG), a three-dimensional mesh of glycan units, is the foundation of th...
Source: The Biochemical Journal - July 27, 2023 Category: Biochemistry Authors: Vijay Soni Eric H Rosenn Ramya Venkataraman Source Type: research
Elevated CO2 alters photosynthesis, growth and susceptibility to powdery mildew of oak seedling
Biochem J. 2023 Jul 27:BCJ20230002. doi: 10.1042/BCJ20230002. Online ahead of print.ABSTRACTElevated CO2 (eCO2) is a determinant factor of climate change and is known to alter plant processes such as physiology, growth and resistance to pathogens. Quercus robur, a tree species integrated in most forest regeneration strategies, shows high vulnerability to powdery mildew (PM) disease at the seedling stage. PM is present in most oak forests and it is considered a bottleneck for oak woodland regeneration. Our study aims to decipher the effect of eCO2 on plant responses to PM. Oak seedlings were grown in controlled environment ...
Source: The Biochemical Journal - July 27, 2023 Category: Biochemistry Authors: Rosa Sanchez-Lucas Carolina Mayoral Mark Raw Maria-Anna Mousouraki Estrella Luna Source Type: research
Insights into the central role of N-acetyl-glucosamine-1-phosphate uridyltransferase (GlmU) in peptidoglycan metabolism and its potential as a therapeutic target
Biochem J. 2023 Jun 18;480(15):1147-1164. doi: 10.1042/BCJ20230173.ABSTRACTSeveral decades after the discovery of the first antibiotic (penicillin) microbes have evolved novel mechanisms of resistance; endangering not only our abilities to combat future bacterial pandemics but many other clinical challenges such as acquired infections during surgeries. Antimicrobial resistance (AMR) is attributed to the mismanagement and overuse of these medications and is complicated by a slower rate of the discovery of novel drugs and targets. Bacterial peptidoglycan (PG), a three-dimensional mesh of glycan units, is the foundation of th...
Source: The Biochemical Journal - July 27, 2023 Category: Biochemistry Authors: Vijay Soni Eric H Rosenn Ramya Venkataraman Source Type: research
Elevated CO2 alters photosynthesis, growth and susceptibility to powdery mildew of oak seedling
Biochem J. 2023 Jul 27:BCJ20230002. doi: 10.1042/BCJ20230002. Online ahead of print.ABSTRACTElevated CO2 (eCO2) is a determinant factor of climate change and is known to alter plant processes such as physiology, growth and resistance to pathogens. Quercus robur, a tree species integrated in most forest regeneration strategies, shows high vulnerability to powdery mildew (PM) disease at the seedling stage. PM is present in most oak forests and it is considered a bottleneck for oak woodland regeneration. Our study aims to decipher the effect of eCO2 on plant responses to PM. Oak seedlings were grown in controlled environment ...
Source: The Biochemical Journal - July 27, 2023 Category: Biochemistry Authors: Rosa Sanchez-Lucas Carolina Mayoral Mark Raw Maria-Anna Mousouraki Estrella Luna Source Type: research
