Comprehensive comparative assessment of the Arabidopsis thaliana MLO2-CALMODULIN2 interaction by various in vitro and in vivo protein-protein interaction assays
Biochem J. 2023 Sep 28:BCJ20230255. doi: 10.1042/BCJ20230255. Online ahead of print.ABSTRACTMildew resistance locus o (MLO) proteins are heptahelical integral membrane proteins of which some isoforms act as susceptibility factors for the powdery mildew pathogen. In many angiosperm plant species, loss-of-function mlo mutants confer durable broad-spectrum resistance against the fungal disease. Barley Mlo is known to interact via a cytosolic carboxyl-terminal domain with the intracellular calcium sensor calmodulin (CAM) in a calcium-dependent manner. Site-directed mutagenesis has revealed key amino acid residues in the barley...
Source: The Biochemical Journal - September 28, 2023 Category: Biochemistry Authors: Kira von Bongartz Bj örn Sabelleck Ane žka Baquero Forero Hannah Kuhn Franz Leissing Ralph Panstruga Source Type: research
Comprehensive comparative assessment of the Arabidopsis thaliana MLO2-CALMODULIN2 interaction by various in vitro and in vivo protein-protein interaction assays
Biochem J. 2023 Sep 28:BCJ20230255. doi: 10.1042/BCJ20230255. Online ahead of print.ABSTRACTMildew resistance locus o (MLO) proteins are heptahelical integral membrane proteins of which some isoforms act as susceptibility factors for the powdery mildew pathogen. In many angiosperm plant species, loss-of-function mlo mutants confer durable broad-spectrum resistance against the fungal disease. Barley Mlo is known to interact via a cytosolic carboxyl-terminal domain with the intracellular calcium sensor calmodulin (CAM) in a calcium-dependent manner. Site-directed mutagenesis has revealed key amino acid residues in the barley...
Source: The Biochemical Journal - September 28, 2023 Category: Biochemistry Authors: Kira von Bongartz Bj örn Sabelleck Ane žka Baquero Forero Hannah Kuhn Franz Leissing Ralph Panstruga Source Type: research
Comprehensive comparative assessment of the Arabidopsis thaliana MLO2-CALMODULIN2 interaction by various in vitro and in vivo protein-protein interaction assays
Biochem J. 2023 Sep 28:BCJ20230255. doi: 10.1042/BCJ20230255. Online ahead of print.ABSTRACTMildew resistance locus o (MLO) proteins are heptahelical integral membrane proteins of which some isoforms act as susceptibility factors for the powdery mildew pathogen. In many angiosperm plant species, loss-of-function mlo mutants confer durable broad-spectrum resistance against the fungal disease. Barley Mlo is known to interact via a cytosolic carboxyl-terminal domain with the intracellular calcium sensor calmodulin (CAM) in a calcium-dependent manner. Site-directed mutagenesis has revealed key amino acid residues in the barley...
Source: The Biochemical Journal - September 28, 2023 Category: Biochemistry Authors: Kira von Bongartz Bj örn Sabelleck Ane žka Baquero Forero Hannah Kuhn Franz Leissing Ralph Panstruga Source Type: research
Comprehensive comparative assessment of the Arabidopsis thaliana MLO2-CALMODULIN2 interaction by various in vitro and in vivo protein-protein interaction assays
Biochem J. 2023 Sep 28:BCJ20230255. doi: 10.1042/BCJ20230255. Online ahead of print.ABSTRACTMildew resistance locus o (MLO) proteins are heptahelical integral membrane proteins of which some isoforms act as susceptibility factors for the powdery mildew pathogen. In many angiosperm plant species, loss-of-function mlo mutants confer durable broad-spectrum resistance against the fungal disease. Barley Mlo is known to interact via a cytosolic carboxyl-terminal domain with the intracellular calcium sensor calmodulin (CAM) in a calcium-dependent manner. Site-directed mutagenesis has revealed key amino acid residues in the barley...
Source: The Biochemical Journal - September 28, 2023 Category: Biochemistry Authors: Kira von Bongartz Bj örn Sabelleck Ane žka Baquero Forero Hannah Kuhn Franz Leissing Ralph Panstruga Source Type: research
Comprehensive comparative assessment of the Arabidopsis thaliana MLO2-CALMODULIN2 interaction by various in vitro and in vivo protein-protein interaction assays
Biochem J. 2023 Sep 28:BCJ20230255. doi: 10.1042/BCJ20230255. Online ahead of print.ABSTRACTMildew resistance locus o (MLO) proteins are heptahelical integral membrane proteins of which some isoforms act as susceptibility factors for the powdery mildew pathogen. In many angiosperm plant species, loss-of-function mlo mutants confer durable broad-spectrum resistance against the fungal disease. Barley Mlo is known to interact via a cytosolic carboxyl-terminal domain with the intracellular calcium sensor calmodulin (CAM) in a calcium-dependent manner. Site-directed mutagenesis has revealed key amino acid residues in the barley...
Source: The Biochemical Journal - September 28, 2023 Category: Biochemistry Authors: Kira von Bongartz Bj örn Sabelleck Ane žka Baquero Forero Hannah Kuhn Franz Leissing Ralph Panstruga Source Type: research
ISGylation-independent protection of cell growth by USP18 following interferon stimulation
Biochem J. 2023 Sep 26:BCJ20230301. doi: 10.1042/BCJ20230301. Online ahead of print.ABSTRACTType 1 interferon stimulation highly up-regulates all elements of a ubiquitin-like conjugation system that leads to ISGylation of target proteins. An ISG15-specific member of the deubiquitylase family, USP18, is up-regulated in a co-ordinated manner. USP18 can also provide a negative feedback by inhibiting JAK-STAT signaling through protein interactions independently of DUB activity. Here, we provide an acute example of this phenomenon, whereby the early expression of USP18, post-interferon treatment of HCT116 colon cancer cells is ...
Source: The Biochemical Journal - September 27, 2023 Category: Biochemistry Authors: Anne Clancy Emma V Rusilowicz-Jones Iona Wallace Kirby Swatek Sylvie Urbe Michael J Clague Source Type: research
ISGylation-independent protection of cell growth by USP18 following interferon stimulation
Biochem J. 2023 Sep 26:BCJ20230301. doi: 10.1042/BCJ20230301. Online ahead of print.ABSTRACTType 1 interferon stimulation highly up-regulates all elements of a ubiquitin-like conjugation system that leads to ISGylation of target proteins. An ISG15-specific member of the deubiquitylase family, USP18, is up-regulated in a co-ordinated manner. USP18 can also provide a negative feedback by inhibiting JAK-STAT signaling through protein interactions independently of DUB activity. Here, we provide an acute example of this phenomenon, whereby the early expression of USP18, post-interferon treatment of HCT116 colon cancer cells is ...
Source: The Biochemical Journal - September 27, 2023 Category: Biochemistry Authors: Anne Clancy Emma V Rusilowicz-Jones Iona Wallace Kirby Swatek Sylvie Urbe Michael J Clague Source Type: research
Incorporation of inosine into DNA by human polymerase eta (Pol η): kinetics of nucleotide misincorporation and structural basis for the mutagenicity
This study demonstrates that TLS incorporation of inosine monophosphate (IMP) into growing DNA chains contributes to its mutagenic potential in cells.PMID:37746864 | DOI:10.1042/BCJ20230159 (Source: The Biochemical Journal)
Source: The Biochemical Journal - September 25, 2023 Category: Biochemistry Authors: Qi Zhang Natalia Tretyakova Source Type: research
Functional annotation of haloacid dehalogenase superfamily structural genomics proteins
Biochem J. 2023 Sep 23:BCJ20230057. doi: 10.1042/BCJ20230057. Online ahead of print.ABSTRACTHaloacid dehalogenases (HAD) are members of a large superfamily that includes many Structural Genomics proteins with poorly characterized functionality. This superfamily consists of multiple types of enzymes that can act as sugar phosphatases, haloacid dehalogenases, phosphonoacetaldehyde hydrolases, ATPases, or phosphate monoesterases. Here we report on predicted functional annotations and experimental testing by direct biochemical assay for Structural Genomics proteins from the HAD superfamily. To characterize the functions of HAD...
Source: The Biochemical Journal - September 25, 2023 Category: Biochemistry Authors: Lakindu S Pathira Kankanamge Lydia A Ruffner Mong Mary Touch Manuel Pina Penny J Beuning Mary Jo Ondrechen Source Type: research
Genetic modulation of CWD prion propagation in cervid PrP Drosophila
Biochem J. 2023 Sep 25:BCJ20230247. doi: 10.1042/BCJ20230247. Online ahead of print.ABSTRACTChronic wasting disease is a fatal prion condition of cervids such as deer, elk, moose and reindeer. Secretion and excretion of prion infectivity from North American cervids with this condition causes environmental contamination and subsequent efficient lateral transmission in free-ranging and farmed cervids. Variants of cervid PrP exist that affect host susceptibility to chronic wasting disease. Cervid breeding programmes aimed at increasing the frequency of PrP variants associated with resistance to chronic wasting disease may red...
Source: The Biochemical Journal - September 25, 2023 Category: Biochemistry Authors: Alana M Thackray Erin E McNulty Amy V Nalls Alzbeta Cardova Linh Tran Glenn Telling Sylvie L Benestad Sabine Gilch Candace K Mathiason Raymond Bujdoso Source Type: research
Proteotoxic stresses stimulate dissociation of UBL4A from the tail-anchored protein recognition complex
In this study, we found that disease-associated inclusions induced by elongated polyglutamine chains disrupt the complex formation of BAG6 with UBL4A, a mammalian homologue of yeast Get5. UBL4A also dissociated from BAG6 in response to proteotoxic stresses such as proteasomal inhibition and mitochondrial depolarization. These findings imply that the cytotoxicity of pathological protein aggregates might be attributed in part to disruption of the BAG6-UBL4A complex that is required for the biogenesis of tail-anchored proteins.PMID:37747814 | DOI:10.1042/BCJ20230267 (Source: The Biochemical Journal)
Source: The Biochemical Journal - September 25, 2023 Category: Biochemistry Authors: Hiroyuki Kawahara Takumi Hagiwara Ryosuke Minami Chizuru Ushio Naoto Yokota Source Type: research
Incorporation of inosine into DNA by human polymerase eta (Pol η): kinetics of nucleotide misincorporation and structural basis for the mutagenicity
This study demonstrates that TLS incorporation of inosine monophosphate (IMP) into growing DNA chains contributes to its mutagenic potential in cells.PMID:37746864 | DOI:10.1042/BCJ20230159 (Source: The Biochemical Journal)
Source: The Biochemical Journal - September 25, 2023 Category: Biochemistry Authors: Qi Zhang Natalia Tretyakova Source Type: research
Functional annotation of haloacid dehalogenase superfamily structural genomics proteins
Biochem J. 2023 Sep 23:BCJ20230057. doi: 10.1042/BCJ20230057. Online ahead of print.ABSTRACTHaloacid dehalogenases (HAD) are members of a large superfamily that includes many Structural Genomics proteins with poorly characterized functionality. This superfamily consists of multiple types of enzymes that can act as sugar phosphatases, haloacid dehalogenases, phosphonoacetaldehyde hydrolases, ATPases, or phosphate monoesterases. Here we report on predicted functional annotations and experimental testing by direct biochemical assay for Structural Genomics proteins from the HAD superfamily. To characterize the functions of HAD...
Source: The Biochemical Journal - September 25, 2023 Category: Biochemistry Authors: Lakindu S Pathira Kankanamge Lydia A Ruffner Mong Mary Touch Manuel Pina Penny J Beuning Mary Jo Ondrechen Source Type: research
Genetic modulation of CWD prion propagation in cervid PrP Drosophila
Biochem J. 2023 Sep 25:BCJ20230247. doi: 10.1042/BCJ20230247. Online ahead of print.ABSTRACTChronic wasting disease is a fatal prion condition of cervids such as deer, elk, moose and reindeer. Secretion and excretion of prion infectivity from North American cervids with this condition causes environmental contamination and subsequent efficient lateral transmission in free-ranging and farmed cervids. Variants of cervid PrP exist that affect host susceptibility to chronic wasting disease. Cervid breeding programmes aimed at increasing the frequency of PrP variants associated with resistance to chronic wasting disease may red...
Source: The Biochemical Journal - September 25, 2023 Category: Biochemistry Authors: Alana M Thackray Erin E McNulty Amy V Nalls Alzbeta Cardova Linh Tran Glenn Telling Sylvie L Benestad Sabine Gilch Candace K Mathiason Raymond Bujdoso Source Type: research
Proteotoxic stresses stimulate dissociation of UBL4A from the tail-anchored protein recognition complex
In this study, we found that disease-associated inclusions induced by elongated polyglutamine chains disrupt the complex formation of BAG6 with UBL4A, a mammalian homologue of yeast Get5. UBL4A also dissociated from BAG6 in response to proteotoxic stresses such as proteasomal inhibition and mitochondrial depolarization. These findings imply that the cytotoxicity of pathological protein aggregates might be attributed in part to disruption of the BAG6-UBL4A complex that is required for the biogenesis of tail-anchored proteins.PMID:37747814 | DOI:10.1042/BCJ20230267 (Source: The Biochemical Journal)
Source: The Biochemical Journal - September 25, 2023 Category: Biochemistry Authors: Hiroyuki Kawahara Takumi Hagiwara Ryosuke Minami Chizuru Ushio Naoto Yokota Source Type: research
