Recombinase-Aided Loop-Mediated Isothermal Amplification on Human Plasmodium knowlesi
In this study, we report the utilization of recombinase-aided LAMP to specifically amplify the 18S sRNA of Plasmodium knowlesi. The method was built on a conventional LAMP assay by inclusion of an extra enzyme, namely recombinase, into the master mixture. With the addition of recombinase into the LAMP assay, the assay speed was executed within a time frame of less than 28 minutes at 65°C. We screened 55 P. knowlesi samples and 47 non-P. knowlesi samples. No cross-reactivity was observed for non-P. knowlesi samples, and the detection limit for recombinase-aided LAMP was one copy for P. knowlesi after LAMP amplification. It...
Source: The American Journal of Tropical Medicine and Hygiene - February 27, 2024 Category: Tropical Medicine Authors: Meng Yee Lai Mohd Hafizi Abdul Hamid Jenarun Jelip Rose Nani Mudin Yee Ling Lau Source Type: research

Historical Hot Spots of Dengue and Zika Viruses to Guide Targeted Vector Control in San Juan, Puerto Rico (2010-2022)
Am J Trop Med Hyg. 2024 Feb 27:tpmd230627. doi: 10.4269/ajtmh.23-0627. Online ahead of print.ABSTRACTDengue viruses (DENV) continue to cause large outbreaks in tropical countries, while chikungunya and Zika (ZIKV) viruses have added complexity to Aedes-borne disease prevention and control efforts. Because these viruses are transmitted by the same vectors in urban areas, it is useful to understand if sequential outbreaks caused by these viruses have commonalities, such as similar seasonal and spatial patterns, that would help anticipate and perhaps prevent future outbreaks. We explored and analyzed the heterogeneity of conf...
Source: The American Journal of Tropical Medicine and Hygiene - February 27, 2024 Category: Tropical Medicine Authors: Roberto Barrera Jose Ruiz Laura E Adams Melissa Marzan-Rodriguez Gabriela Paz-Bailey Source Type: research

A Quiet Hero, An Epic History
Am J Trop Med Hyg. 2023 Dec 26;110(2):413-416. doi: 10.4269/ajtmh.23-0853.NO ABSTRACTPMID:38382102 | DOI:10.4269/ajtmh.23-0853 (Source: The American Journal of Tropical Medicine and Hygiene)
Source: The American Journal of Tropical Medicine and Hygiene - February 21, 2024 Category: Tropical Medicine Authors: Claire Panosian Dunavan Source Type: research

Etiology of Acute Febrile Illnesses in Adults in the Defense Community in French Guiana
We describe the prevalence of different etiologies of acute febrile illnesses occurring among French service members and their families, excluding children, in general practice in French Guiana. From June 2017 to March 2020, patients with a fever ≥ 37.8°C with a duration of less than 15 days who sought medical care at the army medical centers in Cayenne and Kourou were prospectively enrolled. Based on clinical presentation, blood, urine, nasopharyngeal, and stool samples were collected for diagnostic testing for viruses, bacteria, and parasites (by direct examination, microscopic examination of blood smears, culture, se...
Source: The American Journal of Tropical Medicine and Hygiene - February 20, 2024 Category: Tropical Medicine Authors: Guillaume Velut Franck de Laval Morgane Berry Fr édérique Dufour Gaume Nathalie Andr é Lo ïc Epelboin Anne Lavergne Antoine Enfissi Felix Djossou Dominique Rousset S ébastien Briolant Source Type: research

Impact of Rotaviral Diarrhea on Child Growth in Sub-Saharan Africa and South Asia in the Global Enteric Multicenter Study
Am J Trop Med Hyg. 2024 Feb 20:tpmd230406. doi: 10.4269/ajtmh.23-0406. Online ahead of print.ABSTRACTRotavirus is the leading cause of dehydrating diarrhea among children in developing countries. The impact of rotaviral diarrhea on nutritional status is not well understood. We aimed to determine the association between rotavirus-positive moderate-to-severe diarrhea and nutrition in children under 5 years of age. We analyzed data from the Global Enteric Multicenter Study on children 0-59 months old from South Asia and sub-Saharan Africa. The relationships between explanatory variables and outcome variables were assessed usi...
Source: The American Journal of Tropical Medicine and Hygiene - February 20, 2024 Category: Tropical Medicine Authors: Rukaeya Amin Sobi Al-Afroza Sultana Soroar Hossain Khan Md Ahshanul Haque Sharika Nuzhat Md Nasif Hossain Pradip K Bardhan Mohammod Jobayer Chisti Subhra Chakraborty Tahmeed Ahmed Rina Das Abu S G Faruque Source Type: research

Efficacy of X-Ray Phytosanitary Irradiation on the Infectivity and Reproduction of Angiostrongylus cantonensis in Experimentally Infected Rats
Am J Trop Med Hyg. 2024 Feb 20:tpmd230570. doi: 10.4269/ajtmh.23-0570. Online ahead of print.ABSTRACTAngiostrongylus cantonensis is a globally distributed nematode and the leading cause of eosinophilic meningitis in humans. As a global hotspot for this disease, Hawaii's agricultural exports may be contributing to the spread of A. cantonensis. Phytosanitary irradiation doses of 150 or 400 Gy provide quarantine security against multiple insect pests. We evaluated the in vitro and in vivo effects of phytosanitary irradiation on infectious, third-stage, A. cantonensis larvae. In vitro experiments directly exposed larvae to irr...
Source: The American Journal of Tropical Medicine and Hygiene - February 20, 2024 Category: Tropical Medicine Authors: Susan I Jarvi Lisa M Kaluna Steven C Hess Lindsey Hamilton Carmen Antaky Robert T Sugihara Israel L Leinbach Yaeko Tagami Argon Steel Kathleen Howe Steven Jacquier Forrest Cookman Jocelyn Diaz John Jacob Peter Follett Source Type: research

Roaming Dogs, Intense Brown Dog Tick Infestation, and Emerging Rocky Mountain Spotted Fever in Tijuana, M éxico
Am J Trop Med Hyg. 2024 Feb 20:tpmd230410. doi: 10.4269/ajtmh.23-0410. Online ahead of print.ABSTRACTA two decades-long epidemic of Rocky Mountain spotted fever in northern México reached the U.S. border city of Tijuana in 2021. Cases were near the city periphery in marginalized areas, some lacking infrastructure such as streets or utilities. We worked in the three census areas where human cases were reported and in 12 additional control Áreas Geoestadisticas Básicas. There were dogs, the primary tick host and Rickettsia rickettsii reservoir, in 76% of homes, with 2.2 owned dogs per home on average, approximately equal ...
Source: The American Journal of Tropical Medicine and Hygiene - February 20, 2024 Category: Tropical Medicine Authors: Janet Foley Andr és M López-Pérez Francesca Rubino Laura Backus Cusi Ferradas Julio Barr ón-Rodriguez Hugo Mendoza Rodolfo Arroyo-Machado Leslie C Inustroza-S ánchez Oscar E Zazueta Source Type: research

Disseminated Tuberculosis of the Axial Skeleton
Am J Trop Med Hyg. 2024 Feb 20:tpmd230691. doi: 10.4269/ajtmh.23-0691. Online ahead of print.NO ABSTRACTPMID:38377610 | DOI:10.4269/ajtmh.23-0691 (Source: The American Journal of Tropical Medicine and Hygiene)
Source: The American Journal of Tropical Medicine and Hygiene - February 20, 2024 Category: Tropical Medicine Authors: Gawahir A Ali Wael Goravey Source Type: research

Detection of Artemisinin Resistance Marker Kelch-13 469Y in Plasmodium falciparum, South Kivu, Democratic Republic of the Congo, 2022
Am J Trop Med Hyg. 2024 Feb 20:tpmd230740. doi: 10.4269/ajtmh.23-0740. Online ahead of print.ABSTRACTPartial artemisinin resistance has emerged in East Africa, posing a threat to malaria control across the continent. The Democratic Republic of the Congo carries one of the heaviest malaria burdens globally, and the South Kivu province directly borders current artemisinin resistance hot spots, but indications of such resistance have not been observed so far. We assessed molecular markers of antimalarial drug resistance in 256 Plasmodium falciparum isolates collected in 2022 in South Kivu, Democratic Republic of the Congo. On...
Source: The American Journal of Tropical Medicine and Hygiene - February 20, 2024 Category: Tropical Medicine Authors: Welmoed van Loon Bertin C Bisimwa Val éry Byela Rebecca Kirby Patrick M Bugeme Aim é Balagizi David Lupande Espoir B Malembaka Frank P Mockenhaupt Esto Bahizire Source Type: research

Burden of Submicroscopic Plasmodium Infections and Detection of kelch13 Mutant Parasites in Military and Civilian Populations in Papua New Guinea
Am J Trop Med Hyg. 2024 Feb 20:tpmd230508. doi: 10.4269/ajtmh.23-0508. Online ahead of print.ABSTRACTMalaria remains a major public health problem in Papua New Guinea (PNG) and an important force health protection issue for both PNG and Australian Defence Forces. To investigate the malaria burden in the military and civilians residing on military bases, a cross-sectional survey was conducted in April 2019 at three military bases in Wewak, Manus Island, and Vanimo, PNG. A total of 1,041 participants were enrolled; 235 military personnel from three bases and 806 civilians from Wewak and Vanimo. Polymerase chain reaction (PCR...
Source: The American Journal of Tropical Medicine and Hygiene - February 20, 2024 Category: Tropical Medicine Authors: Paul A Pickering Ivor Harris David Smith Fiona McCallum Peter Kaminiel Alyson Auliff Qin Cheng Source Type: research

Evidence of Ongoing Transmission of Zika Virus in M érida, Mexico
Am J Trop Med Hyg. 2024 Feb 20:tpmd230533. doi: 10.4269/ajtmh.23-0533. Online ahead of print.ABSTRACTSince the Zika virus (ZIKV) pandemic in 2015-2017, there has been a near absence of reported cases in the Americas outside of Brazil. However, the conditions for Aedes-borne transmission persist in Latin America, and the threat of ZIKV transmission is increasing as population immunity wanes. Mexico has reported only 70 cases of laboratory-confirmed ZIKV infection since 2020, with no cases recorded in the Yucatán peninsula. Here, we provide evidence of active ZIKV transmission, despite the absence of official case reports, ...
Source: The American Journal of Tropical Medicine and Hygiene - February 20, 2024 Category: Tropical Medicine Authors: James T Earnest Karina Jacqueline Ciau-Carillo Oscar D Kirstein Azael Che-Mendoza Daniel O Espinoza Henry Puerta-Guardo Kevin Yam-Trujillo Manuel Parra-Carde ña Gloria A Barrera-Fuentes Norma Pavia-Ruz Fabian Correa-Morales Hector Gomez-Dantes Pilar Gran Source Type: research

Etiology of Acute Febrile Illnesses in Adults in the Defense Community in French Guiana
We describe the prevalence of different etiologies of acute febrile illnesses occurring among French service members and their families, excluding children, in general practice in French Guiana. From June 2017 to March 2020, patients with a fever ≥ 37.8°C with a duration of less than 15 days who sought medical care at the army medical centers in Cayenne and Kourou were prospectively enrolled. Based on clinical presentation, blood, urine, nasopharyngeal, and stool samples were collected for diagnostic testing for viruses, bacteria, and parasites (by direct examination, microscopic examination of blood smears, culture, se...
Source: The American Journal of Tropical Medicine and Hygiene - February 20, 2024 Category: Tropical Medicine Authors: Guillaume Velut Franck de Laval Morgane Berry Fr édérique Dufour Gaume Nathalie Andr é Lo ïc Epelboin Anne Lavergne Antoine Enfissi Felix Djossou Dominique Rousset S ébastien Briolant Source Type: research

Impact of Rotaviral Diarrhea on Child Growth in Sub-Saharan Africa and South Asia in the Global Enteric Multicenter Study
Am J Trop Med Hyg. 2024 Feb 20:tpmd230406. doi: 10.4269/ajtmh.23-0406. Online ahead of print.ABSTRACTRotavirus is the leading cause of dehydrating diarrhea among children in developing countries. The impact of rotaviral diarrhea on nutritional status is not well understood. We aimed to determine the association between rotavirus-positive moderate-to-severe diarrhea and nutrition in children under 5 years of age. We analyzed data from the Global Enteric Multicenter Study on children 0-59 months old from South Asia and sub-Saharan Africa. The relationships between explanatory variables and outcome variables were assessed usi...
Source: The American Journal of Tropical Medicine and Hygiene - February 20, 2024 Category: Tropical Medicine Authors: Rukaeya Amin Sobi Al-Afroza Sultana Soroar Hossain Khan Md Ahshanul Haque Sharika Nuzhat Md Nasif Hossain Pradip K Bardhan Mohammod Jobayer Chisti Subhra Chakraborty Tahmeed Ahmed Rina Das Abu S G Faruque Source Type: research

Efficacy of X-Ray Phytosanitary Irradiation on the Infectivity and Reproduction of Angiostrongylus cantonensis in Experimentally Infected Rats
Am J Trop Med Hyg. 2024 Feb 20:tpmd230570. doi: 10.4269/ajtmh.23-0570. Online ahead of print.ABSTRACTAngiostrongylus cantonensis is a globally distributed nematode and the leading cause of eosinophilic meningitis in humans. As a global hotspot for this disease, Hawaii's agricultural exports may be contributing to the spread of A. cantonensis. Phytosanitary irradiation doses of 150 or 400 Gy provide quarantine security against multiple insect pests. We evaluated the in vitro and in vivo effects of phytosanitary irradiation on infectious, third-stage, A. cantonensis larvae. In vitro experiments directly exposed larvae to irr...
Source: The American Journal of Tropical Medicine and Hygiene - February 20, 2024 Category: Tropical Medicine Authors: Susan I Jarvi Lisa M Kaluna Steven C Hess Lindsey Hamilton Carmen Antaky Robert T Sugihara Israel L Leinbach Yaeko Tagami Argon Steel Kathleen Howe Steven Jacquier Forrest Cookman Jocelyn Diaz John Jacob Peter Follett Source Type: research

Roaming Dogs, Intense Brown Dog Tick Infestation, and Emerging Rocky Mountain Spotted Fever in Tijuana, M éxico
Am J Trop Med Hyg. 2024 Feb 20:tpmd230410. doi: 10.4269/ajtmh.23-0410. Online ahead of print.ABSTRACTA two decades-long epidemic of Rocky Mountain spotted fever in northern México reached the U.S. border city of Tijuana in 2021. Cases were near the city periphery in marginalized areas, some lacking infrastructure such as streets or utilities. We worked in the three census areas where human cases were reported and in 12 additional control Áreas Geoestadisticas Básicas. There were dogs, the primary tick host and Rickettsia rickettsii reservoir, in 76% of homes, with 2.2 owned dogs per home on average, approximately equal ...
Source: The American Journal of Tropical Medicine and Hygiene - February 20, 2024 Category: Tropical Medicine Authors: Janet Foley Andr és M López-Pérez Francesca Rubino Laura Backus Cusi Ferradas Julio Barr ón-Rodriguez Hugo Mendoza Rodolfo Arroyo-Machado Leslie C Inustroza-S ánchez Oscar E Zazueta Source Type: research