High-mannose-type glycan of basigin in endothelial cells is essential for the opening of blood-brain barrier induced by hypoxia, cyclophilin A or TNF- α
Am J Pathol. 2023 Nov 29:S0002-9440(23)00446-7. doi: 10.1016/j.ajpath.2023.11.007. Online ahead of print.ABSTRACTPathological opening of blood-brain barrier accelerates the progression of various neural diseases. As an essential molecule for the opening of blood-brain barrier, we have specified basigin, a highly glycosylated transmembrane molecule, in barrier-forming endothelial cells. Here, we analyzed the involvement of basigin in the regulation of blood-brain barrier focusing on its glycosylation forms. First, basigin was found to be expressed as the cell surface molecules with complex-type glycan as well as those with ...
Source: The American Journal of Pathology - December 1, 2023 Category: Pathology Authors: Dan Cui Kazuo Yamamoto Eiji Ikeda Source Type: research
High-mannose-type glycan of basigin in endothelial cells is essential for the opening of blood-brain barrier induced by hypoxia, cyclophilin A or TNF- α
Am J Pathol. 2023 Nov 29:S0002-9440(23)00446-7. doi: 10.1016/j.ajpath.2023.11.007. Online ahead of print.ABSTRACTPathological opening of blood-brain barrier accelerates the progression of various neural diseases. As an essential molecule for the opening of blood-brain barrier, we have specified basigin, a highly glycosylated transmembrane molecule, in barrier-forming endothelial cells. Here, we analyzed the involvement of basigin in the regulation of blood-brain barrier focusing on its glycosylation forms. First, basigin was found to be expressed as the cell surface molecules with complex-type glycan as well as those with ...
Source: The American Journal of Pathology - December 1, 2023 Category: Pathology Authors: Dan Cui Kazuo Yamamoto Eiji Ikeda Source Type: research
High-mannose-type glycan of basigin in endothelial cells is essential for the opening of blood-brain barrier induced by hypoxia, cyclophilin A or TNF- α
Am J Pathol. 2023 Nov 29:S0002-9440(23)00446-7. doi: 10.1016/j.ajpath.2023.11.007. Online ahead of print.ABSTRACTPathological opening of blood-brain barrier accelerates the progression of various neural diseases. As an essential molecule for the opening of blood-brain barrier, we have specified basigin, a highly glycosylated transmembrane molecule, in barrier-forming endothelial cells. Here, we analyzed the involvement of basigin in the regulation of blood-brain barrier focusing on its glycosylation forms. First, basigin was found to be expressed as the cell surface molecules with complex-type glycan as well as those with ...
Source: The American Journal of Pathology - December 1, 2023 Category: Pathology Authors: Dan Cui Kazuo Yamamoto Eiji Ikeda Source Type: research
Maternal obesogenic diet attenuates microbiome dependent offspring weaning reaction with worsening of steatotic liver disease
Am J Pathol. 2023 Nov 27:S0002-9440(23)00445-5. doi: 10.1016/j.ajpath.2023.11.006. Online ahead of print.ABSTRACTThe mechanisms by which maternal obesity increases the susceptibility to steatotic liver disease (SLD) in offspring are incompletely understood and models using different maternal obesogenic diets (MODEs) display phenotypic variability, likely reflecting the influence of timing and diet composition. Here we compare three maternal obesogenic diets using standardized exposure times to identify differences in offspring disease progression. We found that the severity of hepatic inflammation and fibrosis in offspring...
Source: The American Journal of Pathology - November 29, 2023 Category: Pathology Authors: Vung Lian Holly Hinrichs Monica Young Austin Faerber O ğuz Özler Yan Xie Samuel J Ballentine Phillip I Tarr Nicholas O Davidson Michael D Thompson Source Type: research
Non-Metastatic Axillary Lymph Nodes Have Distinct Morphology and Immunophenotype in Obese Breast Cancer patients at Risk for Metastasis
This study uses a deep learning model to identify morphological differences in non-metastatic axillary nodes between obese, node-positive and node-negative breast cancer patients. The model was developed using nested cross-validation on 180 cases and achieved an AUC of 0.67 in differentiating patients using hematoxylin and eosin stained whole-slide images. The top predictive patches from the slides according to the model were reviewed by a pathologist and their morphological differences were quantified. This analysis showed an increased average adipocyte size (p-value=0.004), increased white space between lymphocytes (p-va...
Source: The American Journal of Pathology - November 29, 2023 Category: Pathology Authors: Qingyuan Song Kristen E Muller Liesbeth M Hondelink Roberta M diFlorio-Alexander Margaret Karagas Saeed Hassanpour Source Type: research
Multilamellated basement membranes in the capillary network of alveolar capillary dysplasia
This study provides insight into the ultrastructure and pathophysiology of ACD. The lack of normally developed lung capillaries seems to cause a replacement by COL15A1+ vessels, a mechanism recently described in interstitial lung disease. The VIT loss and FN1 overexpression might contribute to the unique appearance of basement membranes in ACD. Future studies are needed to explore the therapeutic potential of downregulating the expression of FN1 and balancing of VIT deficiency.PMID:38029923 | DOI:10.1016/j.ajpath.2023.10.012 (Source: The American Journal of Pathology)
Source: The American Journal of Pathology - November 29, 2023 Category: Pathology Authors: Jan C Kamp Lavinia Neubert Jonas C Schupp Peter Braubach Christoph Wrede Florian Laenger Tim Salditt Jakob Reichmann Tobias Welte Arjang Ruhparwar Fabio Ius Nicolaus Schwerk Anke K Bergmann Sandra von Hardenberg Matthias Griese Christina Rapp Karen M Olss Source Type: research
Maternal obesogenic diet attenuates microbiome dependent offspring weaning reaction with worsening of steatotic liver disease
Am J Pathol. 2023 Nov 27:S0002-9440(23)00445-5. doi: 10.1016/j.ajpath.2023.11.006. Online ahead of print.ABSTRACTThe mechanisms by which maternal obesity increases the susceptibility to steatotic liver disease (SLD) in offspring are incompletely understood and models using different maternal obesogenic diets (MODEs) display phenotypic variability, likely reflecting the influence of timing and diet composition. Here we compare three maternal obesogenic diets using standardized exposure times to identify differences in offspring disease progression. We found that the severity of hepatic inflammation and fibrosis in offspring...
Source: The American Journal of Pathology - November 29, 2023 Category: Pathology Authors: Vung Lian Holly Hinrichs Monica Young Austin Faerber O ğuz Özler Yan Xie Samuel J Ballentine Phillip I Tarr Nicholas O Davidson Michael D Thompson Source Type: research
Non-Metastatic Axillary Lymph Nodes Have Distinct Morphology and Immunophenotype in Obese Breast Cancer patients at Risk for Metastasis
This study uses a deep learning model to identify morphological differences in non-metastatic axillary nodes between obese, node-positive and node-negative breast cancer patients. The model was developed using nested cross-validation on 180 cases and achieved an AUC of 0.67 in differentiating patients using hematoxylin and eosin stained whole-slide images. The top predictive patches from the slides according to the model were reviewed by a pathologist and their morphological differences were quantified. This analysis showed an increased average adipocyte size (p-value=0.004), increased white space between lymphocytes (p-va...
Source: The American Journal of Pathology - November 29, 2023 Category: Pathology Authors: Qingyuan Song Kristen E Muller Liesbeth M Hondelink Roberta M diFlorio-Alexander Margaret Karagas Saeed Hassanpour Source Type: research
Multilamellated basement membranes in the capillary network of alveolar capillary dysplasia
This study provides insight into the ultrastructure and pathophysiology of ACD. The lack of normally developed lung capillaries seems to cause a replacement by COL15A1+ vessels, a mechanism recently described in interstitial lung disease. The VIT loss and FN1 overexpression might contribute to the unique appearance of basement membranes in ACD. Future studies are needed to explore the therapeutic potential of downregulating the expression of FN1 and balancing of VIT deficiency.PMID:38029923 | DOI:10.1016/j.ajpath.2023.10.012 (Source: The American Journal of Pathology)
Source: The American Journal of Pathology - November 29, 2023 Category: Pathology Authors: Jan C Kamp Lavinia Neubert Jonas C Schupp Peter Braubach Christoph Wrede Florian Laenger Tim Salditt Jakob Reichmann Tobias Welte Arjang Ruhparwar Fabio Ius Nicolaus Schwerk Anke K Bergmann Sandra von Hardenberg Matthias Griese Christina Rapp Karen M Olss Source Type: research
Substance P Increases STAT6-Mediated Transcription Activation of Lymphocyte Cytosolic Protein 2 to Sustain M2 Macrophage Predominance in Pediatric Asthma
This study investigates the effect of SP on macrophage phenotype in pediatric asthma and the underpinning factors. Asthmatic children exhibited an increased level of SP, along with a higher proportion of M2 macrophages in their bronchoalveolar lavage fluid. Flow cytometry revealed that SP treatment enhanced the M2 polarization of 12-O-tetradecanoylphorbol 13-acetate-treated THP-1 cells (macrophages) in vitro. By contrast, the administration of a neutralizing antibody of SP reduced the M2 macrophage population, mitigated inflammatory cell infiltration in mouse lung tissues, and decreased the population of immune cells in th...
Source: The American Journal of Pathology - November 23, 2023 Category: Pathology Authors: Miao Li Xiao Zhong Wenting Xu Source Type: research
Substance P Increases STAT6-Mediated Transcription Activation of Lymphocyte Cytosolic Protein 2 to Sustain M2 Macrophage Predominance in Pediatric Asthma
This study investigates the effect of SP on macrophage phenotype in pediatric asthma and the underpinning factors. Asthmatic children exhibited an increased level of SP, along with a higher proportion of M2 macrophages in their bronchoalveolar lavage fluid. Flow cytometry revealed that SP treatment enhanced the M2 polarization of 12-O-tetradecanoylphorbol 13-acetate-treated THP-1 cells (macrophages) in vitro. By contrast, the administration of a neutralizing antibody of SP reduced the M2 macrophage population, mitigated inflammatory cell infiltration in mouse lung tissues, and decreased the population of immune cells in th...
Source: The American Journal of Pathology - November 23, 2023 Category: Pathology Authors: Miao Li Xiao Zhong Wenting Xu Source Type: research
Substance P Increases STAT6-Mediated Transcription Activation of Lymphocyte Cytosolic Protein 2 to Sustain M2 Macrophage Predominance in Pediatric Asthma
This study investigates the effect of SP on macrophage phenotype in pediatric asthma and the underpinning factors. Asthmatic children exhibited an increased level of SP, along with a higher proportion of M2 macrophages in their bronchoalveolar lavage fluid. Flow cytometry revealed that SP treatment enhanced the M2 polarization of 12-O-tetradecanoylphorbol 13-acetate-treated THP-1 cells (macrophages) in vitro. By contrast, the administration of a neutralizing antibody of SP reduced the M2 macrophage population, mitigated inflammatory cell infiltration in mouse lung tissues, and decreased the population of immune cells in th...
Source: The American Journal of Pathology - November 23, 2023 Category: Pathology Authors: Miao Li Xiao Zhong Wenting Xu Source Type: research
Substance P increases STAT6-mediated transcription activation of LCP2 to sustain M2 macrophage predominance in pediatric asthma
This study investigates the effect of SP on macrophage phenotype in pediatric asthma and the underpinning factors. Asthmatic children exhibited an increased level of SP, along with a higher proportion of M2 macrophages in their bronchoalveolar lavage fluid (BALF). Flow cytometry revealed that SP treatment enhanced the M2 polarization of TPA-treated THP-1 cells (macrophages) in vitro. By contrast, the administration of a neutralizing antibody of SP reduced the M2 macrophage population, mitigated inflammatory cell infiltration in mouse lung tissues, and decreased the population of immune cells in the mouse BALF. SP upregulat...
Source: The American Journal of Pathology - November 23, 2023 Category: Pathology Authors: Miao Li Xiao Zhong Wenting Xu Source Type: research
Substance P increases STAT6-mediated transcription activation of LCP2 to sustain M2 macrophage predominance in pediatric asthma
This study investigates the effect of SP on macrophage phenotype in pediatric asthma and the underpinning factors. Asthmatic children exhibited an increased level of SP, along with a higher proportion of M2 macrophages in their bronchoalveolar lavage fluid (BALF). Flow cytometry revealed that SP treatment enhanced the M2 polarization of TPA-treated THP-1 cells (macrophages) in vitro. By contrast, the administration of a neutralizing antibody of SP reduced the M2 macrophage population, mitigated inflammatory cell infiltration in mouse lung tissues, and decreased the population of immune cells in the mouse BALF. SP upregulat...
Source: The American Journal of Pathology - November 23, 2023 Category: Pathology Authors: Miao Li Xiao Zhong Wenting Xu Source Type: research
Insight into the role of gut microbiota in Duchenne muscular dystrophy: an age-related study in mdx mice
This study highlights a specific gut microbiota composition and points out inter-organ interactions in mdx physiopathology with gut microbiota as potential central metabolic organ.PMID:37981219 | DOI:10.1016/j.ajpath.2023.10.010 (Source: The American Journal of Pathology)
Source: The American Journal of Pathology - November 19, 2023 Category: Pathology Authors: Maxence Jollet Mahendra Mariadassou Olivier Ru é Laurence Pessemesse Vincent Ollendorff Sofiane Ramdani Barbara Vernus Anne Bonnieu Christelle Bertrand-Gaday B énédicte Goustard Christelle Koechlin-Ramonatxo Source Type: research
