Electrophysiological characterization of photoreceptor ‐like cells in human inducible pluripotent stem cell‐derived retinal organoids during in vitro maturation
This study provides a comprehensive dataset describing the electrophysiological maturation of photoreceptor‐like cells in human inducible pluripotent stem cell‐derived ROs for precise and sensitive quality control during RO differentiation. AbstractRetinal organoids (ROs) derived from human inducible pluripotent stem cells (hiPSCs) exhibit considerable therapeutic potential. However, current quality control of ROs during in vitro differentiation is largely limited to the detection of molecular markers, often by immunostaining, polymerase chain reaction (PCR) assays and sequencing, often without proper functional assess...
Source: Stem Cells - March 4, 2021 Category: Stem Cells Authors: Lingyun Li,
Huan Zhao,
Haohuan Xie,
Tasneem Akhtar,
Yichuan Yao,
Yuan Cai,
Kai Dong,
Yonghao Gu,
Jin Bao,
Jutao Chen,
Mei Zhang,
Kai Zhong,
Weiping Xu,
Tian Xue Tags: Tissue ‐Specific Stem Cells Source Type: research
Mesenchymal stem cells regulate type 2 innate lymphoid cells via regulatory T cells through ICOS ‐ICOSL interaction
This study revealed that human MSCs suppressed ILC2s via Treg cells through ICOS‐ICOSL inte raction, which provides further insight to regulate ILC2s in inflammatory disorders.© AlphaMed Press 2021Significance StatementGroup 2 innate lymphoid cells (ILC2s) are increasingly recognized for their ability in the initiation and acceleration of type 2 inflammation. Mesenchymal stem cells (MSCs) have been demonstrated able to alleviate type 2 inflammation by modulating T lymphocyte subsets and decreasing TH2 cytokine expression levels in patients with AR and animal models. However, the effects of MSCs on ILC2s have not been in...
Source: Stem Cells - March 4, 2021 Category: Stem Cells Authors: Xingliang Fan,
Zhi ‐Bin Xu,
Cheng‐Lin Li,
Hong‐Yu Zhang,
Ya‐Qi Peng,
Bi‐Xin He,
Xiao‐Qing Liu,
De‐Hua Chen,
Dong Chen,
Cezmi A. Akdis,
Qing‐Ling Fu Tags: Translational and Clinical Research Source Type: research
Transplanted pluripotent stem cell ‐derived photoreceptor precursors elicit conventional and unusual light responses in mice with advanced retinal degeneration
In this study, we have advanced the field by assessing the full range of light responses that develop following transplantation of pluripoten t stem cell‐derived cone photoreceptors in a mouse model of advanced photoreceptor dystrophy. Our data show that despite a relatively low engraftment yield, cone precursors engraft, resulting in conventional light responses in ganglion cells even at advanced degeneration stages. (Source: Stem Cells)
Source: Stem Cells - March 3, 2021 Category: Stem Cells Authors: Darin Zerti,
Gerrit Hilgen,
Birthe Dorgau,
Joseph Collin,
Marius Ader,
Lyle Armstrong,
Evelyne Sernagor,
Majlinda Lako Tags: Embryonic Stem Cells/Induced Pluripotent Stem Cells Source Type: research
Direct neuronal differentiation of neural stem cells for spinal cord injury repair
Diagram showing the directional neuronal differentiation of neural stem cells (NSCs). A, Spinal cord injury (SCI) microenvironment is unfavorable for the neuronal differentiation of NSCs. B, The application of functional biomaterials, myelin ‐associated inhibitor antagonists, small molecules, regenerative factors, and seed cells was used to reconstruct the SCI microenvironment and facilitate the neuronal differentiation of NSCs. C, The intrinsic neuronal differentiation abilities of NSCs were enhanced to promote neurogenesis after SCI . AbstractSpinal cord injury (SCI) typically results in long ‐lasting functional defi...
Source: Stem Cells - March 3, 2021 Category: Stem Cells Authors: Weiwei Xue,
Caixia Fan,
Bing Chen,
Yannan Zhao,
Zhifeng Xiao,
Jianwu Dai Tags: Tissue ‐Specific Stem Cells Source Type: research
Human induced pluripotent stem cells and CRISPR/Cas ‐mediated targeted genome editing: Platforms to tackle sensorineural hearing loss
Fibroblasts or blood from healthy donors and patients with sensorineural hearing loss (SNHL) are used to derive human induced pluripotent stem cells (hiPSCs) which can be subjected to genome editing, then differentiated into two dimensional (2D) and three dimensional (3D) inner ear cellular models for multiple downstream applications, including functional, mechanistic, therapeutic and drug screening studies AbstractHearing loss (HL) is a major global health problem of pandemic proportions. The most common type of HL is sensorineural hearing loss (SNHL) which typically occurs when cells within the inner ear are damaged. Hum...
Source: Stem Cells - March 3, 2021 Category: Stem Cells Authors: Miodrag Stojkovic,
Dongjun Han,
Minjin Jeong,
Petra Stojkovic,
Konstantina M. Stankovic Tags: Embryonic Stem Cells/Induced Pluripotent Stem Cells Source Type: research
A synthetic mRNA cell reprogramming method using CYCLIN D1 promotes DNA repair generating improved genetically stable human induced pluripotent stem cells
In conclusion, we show synthetic mRNA transfection ofCYCLIN D1 repairs DNA during reprogramming resulting in significantly improved genetically stable footprint in human iPSC, enabling a new cell reprogramming method for more accurate and reliable generation of human iPSC for disease modeling and future clinical applications. (Source: Stem Cells)
Source: Stem Cells - March 3, 2021 Category: Stem Cells Authors: Ana Bel én Alvarez‐Palomo,
Jordi Requena‐Osete,
Raul Delgado‐Morales,
Victoria Moreno‐Manzano,
Carme Grau‐Bove,
Agueda M. Tejera,
Manel Juan Otero,
Carme Barrot,
Irene Santos‐Barriopedro,
Alejandro Vaquero,
Jovita Mezquita‐Pla,
S Tags: Embryonic Stem Cells/Induced Pluripotent Stem Cells Source Type: research
Mesenchymal stromal cell ‐mediated immune regulation: A promising remedy in the therapy of type 2 diabetes mellitus
Activated mesenchymal stromal cells (MSCs) and apoptotic MSCs exert potent immunoregulatory effects mainly via secretome, thus modulating the functions of various immune cells. MSC ‐mediated immunomodulation contributes to Type 2 diabetes mellitus therapies via improving islet function and ameliorating insulin resistance. AbstractType 2 diabetes mellitus (T2DM) is a major threat to global public health, with increasing prevalence as well as high morbidity and mortality, to which immune dysfunction has been recognized as a crucial contributor. Mesenchymal stromal cells (MSCs), obtained from various sources and possessing ...
Source: Stem Cells - March 2, 2021 Category: Stem Cells Authors: Ji Chen,
Chen ‐Xi Zheng,
Yan Jin,
Cheng‐Hu Hu Tags: CONCISE REVIEW Source Type: research
ABCB5+ dermal mesenchymal stromal cells with favorable skin homing and local immunomodulation for recessive dystrophic epidermolysis bullosa treatment
Schema of ABCB5+ dermal mesenchymal stem cell (MSC) (DSC) characteristics compared to bone marrow (BM) ‐MSCs. Potential mechanisms of superior skin homing of ABCB5+ DSCs for use in recessive dystrophic epidermolysis bullosa (RDEB) and regenerative medicine applications. Similarities in immunomodulatory capacity of ABCB5+ DSCs and BM‐MSCs are shown in the middle column including anti‐inflammator y M1 > M2 macrophage skewing. AbstractRecessive dystrophic epidermolysis bullosa (RDEB) is a rare, incurable blistering skin disease caused by biallelic mutations in type VII collagen (C7). Advancements in treatment of...
Source: Stem Cells - March 2, 2021 Category: Stem Cells Authors: Julia Riedl,
Michael Pickett ‐Leonard,
Cindy Eide,
Mark Andreas Kluth,
Christoph Ganss,
Natasha Y. Frank,
Markus H. Frank,
Christen L. Ebens,
Jakub Tolar Tags: Regenerative Medicine Source Type: research
O ‐GlcNAc transferase is required to maintain satellite cell function
O ‐GlcNAcylation is a widespread nutrient sensitive post‐translational modification that satellite cells (SCs) use to maintain homeostasis and proper SC function. Ablation of SC specific OGT impairs adult regenerative myogenesis,in vivo cycling properties, and proliferation, presumably through an HCF1 ‐mediated arrest in the cell cycle. AbstractO ‐GlcNAcylation is a post‐translational modification considered to be a nutrient sensor that reports nutrient scarcity or surplus. Although O‐GlcNAcylation exists in a wide range of cells and/or tissues, its functional role in muscle satellite cells (SCs) remains largel...
Source: Stem Cells - February 26, 2021 Category: Stem Cells Authors: Morgan D. Zumbaugh,
Ashley E. Geiger,
Jing Luo,
Zhengxing Shen,
Hao Shi,
David E. Gerrard Tags: Tissue ‐Specific Stem Cells Source Type: research
