Fluid shear stress promotes embryonic stem cell pluripotency via interplay between β‐catenin and vinculin in bioreactor culture
In this study, we investigated how murine embryonic stem cells (mESCs) sense fluid shear stress and modulate a noncanonical Wnt signaling response to promote pluripotency. mESCs showed higher expression of pluripotency marker genes, Oct4, Sox2, and Nanog in the absence of leukemia inh ibitory factor (LIF) in stirred suspension bioreactors compared to adherent culture, a phenomenon we have termed mechanopluripotency. In bioreactor culture, fluid shear promoted the nuclear translocation of the less well‐known pluripotency regulator β‐catenin and concomitant increase of c‐Myc expression, an upstream regulator of Oct4, ...
Source: Stem Cells - April 23, 2021 Category: Stem Cells Authors: Suman C. Nath,
Bradley Day,
Lane Harper,
Jeffrey Yee,
Charlie Yu ‐Ming Hsu,
Leila Larijani,
Leili Rohani,
Nicholas Duan,
Michael S. Kallos,
Derrick E. Rancourt Tags: Embryonic Stem Cells/Induced Pluripotent Stem Cells Source Type: research
Cancer stem cell ‐targeted therapeutic approaches for overcoming trastuzumabresistance in HER2‐positive breast cancer
Several reported targeted therapies against breast cancer stem cells (BCSCs) to overcome trastuzumab resistance in HER2 ‐positive breast cancer. AbstractApplication of the anti ‐HER2 drug trastuzumab has significantly improved the prognosis of patients with the HER2‐positive subtype of breast cancer. However, 50% of patients withHER2 amplification relapse due to trastuzumab resistance. Accumulating evidence indicates that breast cancer is driven by a small subset of cancer ‐initiating cells or breast cancer stem cells (BCSCs), which have the capacity to self‐renew and differentiate to regenerate the tumor cell hi...
Source: Stem Cells - April 21, 2021 Category: Stem Cells Authors: Yan Qiu,
Libo Yang,
Honghong Liu,
Xiaobo Luo Tags: Cancer Stem Cells Source Type: research
Cover Image
Immunohistochemical analysis of chemokine receptor expression in the mouse olfactory neuroepithelium. CXCR4 (green) was mainly located in neuronal stem cells and immediate neuronal progenitor cells (green); GAP43 –labeled immature neurons (red) and OMP–labeled mature olfactory neurons (white). Neurogenesis in the olfactory epithelium was shown to depend on CXCR4 expression.See Senf et al. beginning on page617. (Source: Stem Cells)
Source: Stem Cells - April 16, 2021 Category: Stem Cells Tags: COVER IMAGE Source Type: research
Issue Information
(Source: Stem Cells)
Source: Stem Cells - April 16, 2021 Category: Stem Cells Tags: ISSUE INFORMATION Source Type: research
A preview of selected articles
(Source: Stem Cells)
Source: Stem Cells - April 16, 2021 Category: Stem Cells Authors: Stuart P. Atkinson Tags: PREVIEWS Source Type: research
Cover Image
Immunohistochemical analysis of chemokine receptor expression in the mouse olfactory neuroepithelium. CXCR4 (green) was mainly located in neuronal stem cells and immediate neuronal progenitor cells (green); GAP43 –labeled immature neurons (red) and OMP–labeled mature olfactory neurons (white). Neurogenesis in the olfactory epithelium was shown to depend on CXCR4 expression.See Senf et al. beginning on page617. (Source: Stem Cells)
Source: Stem Cells - April 16, 2021 Category: Stem Cells Tags: COVER IMAGE Source Type: research
Issue Information
(Source: Stem Cells)
Source: Stem Cells - April 16, 2021 Category: Stem Cells Tags: ISSUE INFORMATION Source Type: research
A preview of selected articles
(Source: Stem Cells)
Source: Stem Cells - April 16, 2021 Category: Stem Cells Authors: Stuart P. Atkinson Tags: PREVIEWS Source Type: research
An aged bone marrow niche restrains rejuvenated hematopoietic stem cells
Model of the influence of the niche on old rejuvenated hematopoietic stem cells (HSCs). Old HSCs can be rejuvenated by Cdc42 activity inhibition, and maintain this youthful function when transplanted in young niches. In contrast, when they encounter an aged niche, they are reverted, at least in part, to old HSCs. This revertion might be due to reduced osteopontin level in aged niches. AbstractAging ‐associated leukemia and aging‐associated immune remodeling are in part caused by aging of hematopoietic stem cells (HSCs). An increase in the activity of the small RhoGTPase cell division control protein 42 (Cdc42) within H...
Source: Stem Cells - April 13, 2021 Category: Stem Cells Authors: Novella Guidi,
Gina Marka,
Vadim Sakk,
Yi Zheng,
Maria Carolina Florian,
Hartmut Geiger Tags: Tissue ‐Specific Stem Cells Source Type: research
Cancer Stem Cell ‐Targeted Therapeutic Approaches for Overcoming Trastuzumab Resistance in HER2‐Positive Breast Cancer
Several reported targeted therapies against BCSCs to overcome trastuzumab resistance in HER2 ‐positive breast cancer. AbstractApplication of the anti ‐HER2 drug trastuzumab has significantly improved the prognosis of patients with the HER2‐positive subtype of breast cancer. However, 50% of patients withHER2 amplification relapse due to trastuzumab resistance. Accumulating evidence indicates that breast cancer is driven by a small subset of cancer ‐initiating cells or breast cancer stem cells (BCSCs), which have the capacity to self‐renew and differentiate to regenerate the tumor cell hierarchy. Increasing data su...
Source: Stem Cells - April 10, 2021 Category: Stem Cells Authors: Yan Qiu,
Libo Yang,
Honghong Liu,
Xiaobo Luo Tags: Cancer Stem Cells Source Type: research
Fluid Shear Stress Promotes Embryonic Stem Cell Pluripotency via Interplay between β‐catenin and Vinculin in Bioreactor Culture
In this study, we investigated how murine embryonic stem cells (mESCs) sense fluid shear stress and modulate a non ‐canonical Wnt signaling response to promote pluripotency. mESCs showed higher expression of pluripotency marker genes, Oct4, Sox2 and Nanog in the absence of LIF in stirred suspension bioreactors compared to adherent culture, a phenomenon we have termed mechanopluripotency. In bioreactor culture, fluid shear promoted the nuclear translocation of the less well‐known pluripotency regulator β‐catenin and concomitant increase of c‐Myc expression, an upstream regulator of Oct4, Sox2 and Nanog. We also obs...
Source: Stem Cells - April 10, 2021 Category: Stem Cells Authors: Suman C. Nath,
Bradley Day,
Lane Harper,
Jeffrey Yee,
Charlie Yu ‐Ming Hsu,
Leila Larijani,
Leili Rohani,
Nicholas Duan,
Michael S. Kallos,
Derrick E. Rancourt Tags: Embryonic Stem Cells/Induced Pluripotent Stem Cells Source Type: research
Macrophages at the nexus of mesenchymal stromal cell potency: The emerging role of chemokine cooperativity
The mesenchymal stromal cell (MSC) and macrophage dyad theorem. MSCs polarize macrophages to an interleukin (IL) ‐10 anti‐inflammatory and regenerative functionality via secretome containing heteroplexed chemokines (and other active factors) or efferocytosis. Both macrophage IL‐10 licensing pathways—MSC function dependent and independent—converge to affect clinical outcomes in inflammatory tissue inj ury syndromes. AbstractPharmacological depletion of macrophages in vivo with liposomal clodronate renders mice unresponsive to adoptive transfer of mesenchymal stromal cells (MSCs) for affecting outcomes of acute inf...
Source: Stem Cells - April 7, 2021 Category: Stem Cells Authors: Jacques Galipeau Tags: Concise Review Source Type: research
