[Adsorption of Airbone Organophosphorus Flame Retardants on Polished Rice Stored in a House].
Authors: Watanabe M, Noguchi M, Hashimoto T, Yoshida S Abstract In order to clarify the actual situation of indoor pollution to polished rice with organophosphorus flame retardants (PFRs) which are ubiquitous in the indoor environment, the pollution of PFRs to polished rice stored in a house for a week was investigated. The survey covered 64 ordinary families in the Osaka region. We analyzed six typical PFRs in 37 rice samples in 2015, and ten typical PFRs in 27 rice samples in 2016. Polished rice was homogenized with acetone-hexane and defatted by hexane-acetonitrile partition. Quantitative analysis for PFRs was p...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - January 25, 2020 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[Development of the Genus and Species Determination Method for Histamine Producing Bacteria Isolated from Fishery Product with High-Resolution Melting Analysis].
Authors: Ohshima C, Sato F, Takahashi H, Kuda T, Kimura B Abstract Histamine in foods with a high histidine content may be produced by bacteria with histidine decarboxylase activity. Consumption of food enriched in histamine can produce symptoms of histamine poisoning that include flushing, headache, and urticaria. The number of histamine poisoning cases in Japan has decreased with developments in food hygiene management technology. However, approximately 10 cases are still reported each year. In addition, there have been cases where histamine was detected in the end products, prompting large product recalls. To pr...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - January 25, 2020 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[Determination of Aspartame, Acesulfame Potassium, and Sucralose in Chewing Gum by Dialysis Extraction].
Authors: Sasaki T, Tahara S, Sakamaki N, Sadamasu Y, Ushiyama K, Monma K, Kobayashi C Abstract The three sweeteners, aspartame, acesulfame potassium, and sucralose, in chewing gum were determined by using dialysis and direct extraction methods. The results revealed that the previously reported dialysis method tended to show poor extraction of aspartame in comparison with the direct-extraction method. The direct extraction also caused operational problems, such as the gum base adhering to the instruments. Therefore, we attempted to improve the dialysis method by changing the dialysate, to which the three sweeteners ...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - January 25, 2020 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[DNA Extraction for Sensitive Detection of Shiga Toxin-Producing Escherichia coli in Food by Real-Time PCR Assays].
Authors: Mori T, Nagao-Sato S, Kishino K, Namba T, Hara-Kudo Y Abstract Alkali-heat DNA extraction, a rapid and economical method, was evaluated for use in the detection of Shiga toxin-producing Escherichia coli in food using real-time PCR assays. Alkali-heat DNA extracts led to highly sensitive detection (102-104 CFU/mL) of stx and O-antigen genes in beef liver, ground beef, sliced pork, cheese, lettuce, radish sprouts, tomato, and spinach, equivalent to the sensitivity obtained using a commercial DNA extraction kit that utilizes proteinase K lysis, and silica membrane purification. Although there were differences...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - January 25, 2020 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[Determination of Zilpaterol in Livestock Products by LC-MS/MS].
Authors: Asakura T, Kitamura M, Ishikawa T, Iida T, Nakazato M, Yasuda K, Nemoto S Abstract An analytical method for the determination of zilpaterol in livestock products was developed. The sample was stirred with n-hexane and n-hexane saturated acetonitrile, and zilpaterol in the sample was extracted with acetonitrile. The extract was cleaned up on a ODS cartridge column (1 g) and SCX cartridge column (500 mg). The LC separation was carried out using an Inertsil ODS-4 column and linear gradient elution with 0.1%formic acid and acetonitrile containing 0.1% formic acid as mobile phase. Detection of MS was carried ou...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - January 22, 2020 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[Quantitative Analysis of Piperine and the Derivatives in Long Pepper Extract by HPLC Using Relative Molar Sensitivity].
Authors: Mizumoto T, Nakano F, Nishizaki Y, Masumoto N, Sugimoto N Abstract A novel method was developed for quantification of five major piperine derivatives (piperanine, piperine, chavicine, isopiperine, and isochavicine) in a hot water extract of long pepper fruit (LPE) using the relative molar sensitivity (RMS) based on the combination of HPLC/UV and 1H- quantitative NMR (1H-qNMR). The RMSs of piperanine, chavicine, isopiperine, and isochavicine to piperine of which the absolute purity was determined by 1H-qNMR were calculated to be 0.3693, 1.138, 0.9164, and 1.277, respectively. The total amount of piperine de...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - January 22, 2020 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[Qualitative Real-Time PCR Method for Poisonous Entoloma rhodopolium-Related Species in Japan: Real-Time PCR Method for Entoloma Mushrooms].
Authors: Kondo K, Sakata K, Kato R, Sugano Y, Takeuchi S, Sato M Abstract Qualitative real-time PCR method for three poisonous Entoloma rhodopolium-related species in Japan was established using specific primers and FAM, VIC, Texas Red, Cy5-labeled probes. The use of multicolor probes can extend the method to simultaneous detection of different targets. Standard plasmids were constructed as reference materials. Designed primers and probes in the method detect only a target species, and the detection limit was 12.5 copies or below. This indicates it is highly specific and sensitive enough to detect the poisonous mus...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - January 22, 2020 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[Identification and Quantification of 2-Iodo-4-methylphenol in Milk].
This study aimed to identify this odor component, and our findings indicate the off-flavor component to be 2-iodo-4-methylphenol. PMID: 31956241 [PubMed - in process] (Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan)
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - January 22, 2020 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[Study on Fipronil and Other Pesticide Residues in Chicken Eggs Produced and Sold in Japan].
Authors: Hatta S, Odagawa Y, Izumi J, Nakamae S Abstract In some of the EU member countries, Hong Kong, South Korea, and Taiwan, chicken eggs contaminated by fipronil used without authorization were identified and widely reported. The Ministry of Health, Labour and Welfare of Japan stated no contaminated imports had been found in the country while promising to strengthen monitoring chicken eggs coming from those areas. Meanwhile, there are no reports published to show whether or not chicken eggs produced in Japan contain such pesticide. This paper, therefore, examined 50 commercial brands of chicken eggs produced a...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - January 22, 2020 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[Simultaneous LC-MS/MS Determination of 18 Plant Toxins in Beverages].
Authors: Oishi A, Nagatomi Y, Suzuki K Abstract Assuming the intentional adulteration of beverages with plant toxins, an LC-MS/MS method for the simultaneous determination of 18 plant toxins (lycorine, galantamine, ricinine, scopolamine, gelsemine, atropine, colchicine, α-solanine, jervine, α-chaconine, veratramine, mesaconitine, digoxin, protoveratrine A, aconitine, hypaconitine, oleandrin, and digitoxin) was developed. As analytical samples, beer, distilled spirits, blend tea, ready-to-drink (RTD) coffee, and fermented milk drink were selected. The extraction and purification of the analytes were perf...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - September 3, 2019 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[Uncertainty Evaluation and Internal Quality Control of ELISA Test for Food Allergens (in Egg and Milk) Using Control Samples].
Authors: Kuwahara K, Hirao C, Hirano A Abstract We evaluated measurement uncertainty and performed internal quality control of an ELISA test for allergens in egg and milk, using control samples. For the evaluation of measurement uncertainty, the following three important factors were identified: 1) Differences in test-kit lots, 2) Different-day reproducibility, 3) Same-time reproducibility. A three-stage nested design was used, and the combined standard uncertainty of the three factors mentioned above was calculated based on the results obtained. Measurement uncertainty was defined as the expanded uncertainty obtai...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - September 3, 2019 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[Evaluation of Safety Information of the Foods with Function Claims Based on Adverse Event Reports in Information System on Safety and Effectiveness for Health Foods Database].
Authors: Kobayashi E, Nishijima C, Sato Y, Chiba T Abstract Foods with Function Claims are allowed to label health claims based on scientific evidence evaluated by the manufacturers. To prevent health problems caused by inadequate use, the manufacturers should label proper safety information. To evaluate whether safety information is sufficiently provided, we conducted the adverse event review focused on popular functional ingredients using the database; Information system on safety and effectiveness for health food. The data suggested that causal factor of adverse events related to products containing soy isoflavo...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - September 3, 2019 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[Headspace GC/MS Analysis of Residual Solvents in Laminated Films Used for Food Packaging].
Authors: Ozaki A, Kishi E, Ooshima T, Kakutani N, Abe Y, Mutsuga M, Yamano T Abstract Multilayer laminated films may contain organic solvents derived from adhesives, printing inks and so on. A headspace-GC/MS analysis method for the simultaneous determination of 30 substances such as toluene, xylene, acetone, methyl ethyl ketone, ethyl acetate, butyl acetate, methanol, ethanol, was developed. A N,N-dimethyl formamide solution containing an internal standard substance was added to the sample. After leaving overnight at room temperature, the headspace gas was analyzed by GC/MS. This method was applicable to a laminat...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - September 3, 2019 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[Consideration of the Evaporation Residue Test for Plastics Intended to Contact with Oils and Fatty Foods].
Authors: Kawamura Y, Wada T, Yamaguchi M, Mutsuga M Abstract The Japanese Food Sanitation Act designates the evaporation residue test as a specification for plastics that intended to contact with foods. The test conditions and migration limits for oils and fatty foods were considered on the basis of the results obtained from the evaporation residue test according to the Japanese Act and the overall migration test into olive oil according to EN1186-2. The evaporation residue test was conducted using heptane at 25℃ for 1 hour. The results of most samples were under 30 μg/mL although those of high impact polystyr...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - September 3, 2019 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[Characteristics of Microbial Colony Counts on Agar Plates for Food and Microbial Culture Samples].
In this study, therefore, these two points were studied with raw food samples of raw minced beef and chicken and raw milk and microbial culture samples of Escherichia coli, Staphylococcus aureus, and Saccharomyces cerevisiae. Among four major probabilistic distributions, it was found that aerobic plate counts per plate of the foods were well described with negative binomial, Poisson, and normal distributions and that the colony counts per plate of microbial cultures were described well with binomial, Poisson, and normal distributions. The effect of the number of agar plates on the estimation of the mean of colony counts pe...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - September 3, 2019 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[LC/Tribrid Orbitrap Analysis of Phosphodiesterase-5 Inhibitors and Their Analogs as Adulterants in Dietary Supplements].
Authors: Sakamoto M, Minowa K, Kishimoto K, Nakajima J, Suzuki J, Moriyasu T, Fukaya H, Saito K Abstract LC/Tribrid Orbitrap was developed to determine phosphodiesterase-5 (PDE-5) inhibitors and their analogs as adulterants in dietary supplements. High-resolution MS/MS and MS3 spectra of PDE-5 inhibitors and their analogs were obtained by LC/Tribrid Orbitrap using both higher-energy collisional dissociation and collision-induced dissociation. We investigated dietary supplements that claim to enhance men's sexual performance, and detected PDE-5 inhibitors and their analogs. We also estimated the structures of the PD...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - September 3, 2019 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[Current Studies of Staphylococcal Food Poisoning].
Authors: Suzuki Y PMID: 31391408 [PubMed - in process] (Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan)
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - August 10, 2019 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[Changes in Status of Plasticizers Used in Polyvinyl Chloride Toys].
Authors: Abe Y, Kijima A, Yamaguchi M, Ito Y, Mutsuga M, Akiyama H, Sato K Abstract Analytical survey of plasticizers used in about 500 polyvinyl chloride (PVC) toys in Japanese market was performed in 2014. The results obtained were compared with those obtained in 2009. Fifteen types of plasticizers including di (2-ethylhexyl) terephthalate (DEHTP) were detected. These plasticizers were also detected in the products in 2009, suggesting that the types of plasticizers used in PVC toys have not been changed. Among these detected plasticizers, the detection ratios of DEHTP were 60.3 and 73.7% in both designated and no...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - August 10, 2019 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[Prevalence of Antimicrobial Resistance in Escherichia coli Isolated from Retail Meat in Tokyo, Japan].
In conclusion, E. coli isolates from retail meat were most commonly resistant to TC, and CTX resistance was higher in E. coli isolates from imported chicken than in E. coli isolates from domestic chicken. PMID: 31391410 [PubMed - in process] (Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan)
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - August 10, 2019 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[Determination of Antifungal Drugs in Fish and Livestock Products Using LC-MS/MS].
Authors: Nakagawa Y, Kanda M, Hayashi H, Matsushima Y, Ohba Y, Koike H, Nagano C, Sekimura K, Otsuka K, Sasamoto T, Hashimoto T Abstract We developed an analytical method for determining 15 antifungal drugs, 2 antiparasitic drugs, and 3 veterinary drugs in fish and livestock products using LC-MS/MS. First, 50% ethanol was added to their products, and the mixture was homogenized to reduce drug degradation. Thereafter, 20 drugs were extracted from the pretreated sample mixture using acetonitrile. Cleanup was performed using an alumina-N SPE cartridge. Finally, chromatographic separation was performed using a fully po...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - August 10, 2019 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[Determination of Nonvolatile Amines in Foods by HPLC Following Fluorescamine Derivatization].
Authors: Kubota A, Fujii Y, Kaga T, Nishimura K, Ueno KI Abstract A method was developed for the determination of nonvolatile amines, such as histamine, tyramine, putrescine, and cadaverine, in foods. These nonvolatile amines were extracted from a sample with 5% trichloroacetic acid, and the extract was purified using an InertSep MC-1 cartridge column. The four amines were derivatized with fluorescamine, determined by HPLC with a fluorescence detector, and confirmed by LC-MS/MS. The average recoveries (n=5) and the relative standard deviations from 11 foods (pacific saury, dried mackerel, canned mackerel in brine, ...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - August 10, 2019 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[Validation Study of Rapid Analytical Method for Determination of Cyclamate in Various Kinds of Foods by LC-MS/MS].
Authors: Daimon T, Saito K, Takahashi K, Hamada Y Abstract A rapid, sensitive and selective analytical method by LC-MS/MS was developed and validated for the determination of cyclamate in various kinds of foods. The Preparation of test solutions was performed by heat extraction technique in accordance with an official notification method in Japan. We aimed to reduce the matrix effects in LC-MS/MS only by diluting extracts without clean-up using solid phase column. This method was assessed for 30 kinds of foods fortifying cyclamate at the concentration level of 0.5 μg/g. As a result, trueness was 85.0 to 106.6%, ...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - August 10, 2019 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[Application of an Estimation Method of Microbial Concentration of a Food Sample from Qualitative Data].
Authors: Fujikawa H Abstract Microbial concentrations of thermally processed foods are mostly low. Recently we proposed a stochastic method based on the MPN technique for estimating microbial concentration in a food product whose concentration is low. Namely, with the number of target pathogen-positive samples in the total samples of a food product by a qualitative examination, the concentration of the target microbe in the product was estimated with the stochastic method. However the credible interval of the estimate with the method was not yet studied. Also the method was not validated with foods yet. The present...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - April 26, 2019 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[Study of Tea and Coffee on Inactivation of Kudoa septempunctata].
Authors: Ohnishi T, Furuya A, Arai S, Yoshinari T, Goto K, Hara-Kudo Y Abstract The inhibition of Kudoa septempunctata by green tea extract, black tea extract, and coffee extract were studied. Incubation of about 104 Kudoa spores with green tea extract, black tea extract, or coffee extract at 25℃ for 4 hr reduced the survival ratio of Kudoa to 0%. While coffee extract and green tea extract contain approximately 2 and 1 mM of caffeine, respectively, the incubation of Kudoa spores with 2 and 1 mM of caffeine reduced its survival ratio to 68.2 and 93.3%, respectively. Although green tea extract and black tea extract...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - April 26, 2019 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[Development of a Rapid Determination Method for Strontium-90 in Foods Using a Strontium Extraction Column for Use during Emergency Events].
Authors: Nabeshi H, Tsutsumi T, Matsuda R, Hachisuka A, Akiyama H Abstract To ensure food safety during emergency events such as nuclear disasters, we developed a practical rapid determination method for strontium-90 (Sr-90) in foods. Purification of Sr from foods was simplified using a commercial Sr-extraction column. We also reduced the waiting time to achieve radiative equilibrium between Sr-90 and Y-90. Finally, we developed a rapid determination method for Sr-90 that can be completed in about a week. Using the new method, stable Sr recoveries exceeded 85%. The trueness of the method ranged from 109 to 115% and...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - April 26, 2019 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[Determination of Flufenacet and Its Metabolites in Agricultural Products by LC-MS/MS].
Authors: Imai M, Takagi N, Yoshizaki M, Hosokai E, Kobayashi Y Abstract A simultaneous analytical method based on LC-MS/MS was developed for the determination of flufenacet and its metabolites, [(4-fluorophenyl)(1-methylethyl) amino]oxo-acetic acid and [N-(4-fluorophenyl)-N-(1-methylethyl) acetamide]-2-sulfinylacetic acid, in agricultural products. The compounds were extracted from samples with methanol. The crude extracts were purified using Bond Elut C18 and InertSep GC/PSA, then determined by LC-MS/MS. The average recoveries (n=5) from 4 kinds of agricultural products (wheat, soybean, potato and tomato) spiked a...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - March 4, 2019 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[Molecular Epidemiological Analysis of Hepatitis A Viruses Circulating in Tokyo, 2016-2017].
Authors: Oda M, Somura Y, Nagano M, Kimoto K, Okutsu Y, Shinkai T, Sadamasu K Abstract Hepatitis A virus (HAV) is a common infectious agent that causes acute hepatitis worldwide. Since the incubation period of HAV infection is about one month, it is difficult to identify the source of infection based only on medical interviews. Molecular epidemiological analysis of HAV isolated from patients can help to reveal the infection route and to identify diffuse outbreaks caused by common food vehicles. In the present study, samples were collected from 108 cases notified to the active epidemiological investigation system in...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - January 11, 2019 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[Determination of Insect Order by Analyzing Mitochondrial Gene ND5].
Authors: Yamauchi H, Harada M, Tajima R Abstract Polymorphism in the mitochondrial gene ND5 enables the determination of the order, a taxonomic rank, of many insects from the 5 major insect orders-Diptera, Hymenoptera, Hemiptera, Coleoptera, and Lepidoptera. DNA nucleotide sequences of multiple species were determined and more than 19 nucleotides characteristic of each order were identified; this makes it possible to discriminate insects at the order level more precisely and easily than when using conventional techniques. PMID: 30626783 [PubMed - in process] (Source: Shokuhin eiseigaku zasshi. Journal of the Fo...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - January 11, 2019 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[Surveillance of Cadmium Concentration in Chocolate and Cocoa Powder Products Distributed in Japan].
Authors: Kataoka Y, Watanabe T, Hayashi K, Akiyama H Abstract Chocolate and cocoa are manufactured from cacao beans produced by the cacao tree (Theobroma cacao). These products may contain cadmium (Cd), which originates from contaminated soil. Here, we surveyed the Cd concentrations in dark chocolate, milk chocolate, white chocolate and cocoa powder products purchased at retail stores in Japan, using inductively coupled plasma mass spectrometry. The Cd concentrations in these chocolate and cocoa powder products ranged from 0.00021 to 2.3 mg/kg and from 0.015 to 1.8 mg/kg, respectively. A weak positive correlation w...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - January 11, 2019 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[Survey of Aluminium Content of Processed Food Using Baking Powder (2015-2016)].
Authors: Takanashi M, Ogimoto M, Suzuki K, Haneishi N, Shiozawa Y, Tomioka N, Kimura C, Okamura R, Teramura W, Uematsu Y, Monma K, Kobayashi C Abstract The aluminium (Al) content of Japanese confectionery and foods containing flour was investigated. Some of these items were investigated in previous studies, which examined foods that made use of baking powder containing aluminium potassium sulfate (Alum). Al was detected in 41 of the 123 samples at levels ranging from 0.01 (limit of quantitation) to 0.40 mg/g. The detection rate of Al in almost all confectionery (except Japanese confectionery) was decreased as compa...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - January 11, 2019 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[Establishment of Test Methods for Overall Migration Test into Vegetable Oil Applied to Food Contact Materials Intended for Contact with Oils and Fatty Foods].
Authors: Nakanishi T, Kawamura Y, Joichi K, Watanabe Y, Sugimoto T, Abe Y, Mutsuga M Abstract The evaporation residue test designated in the Japanese Food Sanitation Act is used to determine the total migration amount of substances that food contact materials release into foods. Vegetable oil would be the most suitable food simulant for oils and fatty foods, but it is difficult to remove by heating due to its high boiling point, so heptane and 20% ethanol are used as substitute food simulants in the test for plastics and rubbers. The EU has introduced an overall migration test into olive oil for plastics intended...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - November 16, 2018 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

Development of Enzyme-Linked Immunosorbent Assay for Analysis of Total Aflatoxins Based on Monoclonal Antibody Reactive with Aflatoxins B1, B2, G1 and G2.
Authors: Yamasaki T, Miyake S, Sato N, Hirakawa Y, Iwasa S, Narita H, Watanabe T Abstract A direct competitive enzyme-linked immunosorbent assay (dc-ELISA) was developed for the determination of total amount of aflatoxin B1, B2, G1 and G2 (AFB1, AFB2, AFG1 and AFG2), using a mouse monoclonal antibody that shows similar reactivity to each of these AFs. The working range of the developed dc-ELISA was 50-230 pg/mL for AFB1, 50-270 pg/mL for AFB2, 60-390 pg/mL for AFG1 and 65-700 pg/mL for AFG2. The recovery of AFs from spiked roasted peanuts was 98%. Further, when 4 samples actually contaminated with AFB1, AFB2, AFG...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - November 16, 2018 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[Determination of Cyromazine in Livestock Products by LC-MS/MS on an Anion-Cation Exchange Mode ODS Column].
Authors: Okutomi Y, Hayashi H, Matsushima Y, Ohba Y, Nakagawa Y, Koike H, Nagano C, Sekimura K, Kanda M, Hashimoto T Abstract Cyromazine in livestock products was determined using a validated LC-MS/MS method. There are three key points in our methods. First, the extraction was performed with two solutions, methanol and pH 3.0 McIlvaine buffer. The process was optimized for each type of sample. Secondly, cleanup was performed using a reversed-phase and strong cation exchange mixed-mode cartridge. The cartridge was washed with 0.14% ammonium solution. Thirdly, the chromatographic separation was performed on an anio...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - November 16, 2018 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[A Screening Analysis Method for Stevia Sweetener in Processed Foods by LC-MS/MS].
Authors: Miyakawa H, Sugiki M, Tahara S, Yamamoto S, Yamajima Y, Uematsu Y, Monma K Abstract A LC-MS/MS-based screening method was developed for stevia sweetener in processed foods. After extraction of stevia sweetener from processed foods by dialysis, the dialysate was diluted with water, and stevia sweetener was measured by LC-MS/MS. Recovery from 5 kinds of processed foods spiked with 10 mg/kg of stevioside (SS), 10 mg/kg of rebaudioside A (RS), or 100 mg/kg of α-glu-cosyltransferase-treated stevia (Gts) product was excellent, and no interfering peak was observed. Thirty-six commercial processe...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - November 16, 2018 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

Differentiation of Laboratory Strains of Red Flour Beetle Tribolium castaneum Collected from Various Areas.
Authors: Yamauchi H, Harada M, Miyanoshita A Abstract We recently reported polymorphism in the mitochondrial genes of Tribolium castaneum, a stored grain pest. Here, we show that T. castaneum samples from the main island of Japan can be easily differentiated from samples from other areas by using real-time PCR with minor groove binding (MGB) TaqMan probes containing a single nucleotide polymorphism (SNP) to amplify a 179-bp fragment. This assay was also applicable to samples stored at room temperature for 5 months after natural death, and samples whose DNA was artificially degraded by heat, pressure, or gamma-ray i...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - November 16, 2018 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[Verification and Improvement of an Official Method for Determining Chlorophyll Decomposition Products Including Pheophorbide].
Authors: Furukawa Y, Fujimoto A, Ueno A, Ogiso M, Fujita K Abstract The official method described in Direction Notification Kanshoku No. 99 (May 8, 1981) for the determination of chlorophyll decomposition products including pheophorbide was verified and improved in order to overcome several problems. Firstly, extraction with a mortar required improvement because of the difficulty of maintaining equal power for a long time. Secondly, the saturated sodium sulfate reagent caused a red-shift of the absorption maximum wavelength from the measured wavelength given in the official method; consequently, the absorption ...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - November 16, 2018 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[Chlorpyrifos-Methyl, Pirimiphos-Methyl and Fenitrothion Residues in Commercial Wheat Products].
Authors: Watanabe M, Noguchi M, Hashimoto T, Yoshida S Abstract We measured the concentrations of organophosphorus pesticide (chlorpyrifos-methyl, pirimiphos-methyl and fenitrothion) residues in domestic and imported commercial wheat products (flour, noodles, cookies) collected from 2008 to 2016. Chlorpyrifos-methyl and fenitrothion in domestic flour samples were detected in 16 out of 34 samples at levels of <0.001 to 0.016 ppm, and in 14 out of 34 samples at levels of <0.001 to 0.004 ppm. Chlorpyrifos-methyl was detected in 22 out of 38 domestic cookies at levels of <0.001 to 0.054 ppm (median: 0.0...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - November 16, 2018 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

Surveillance of Chlorantraniliprole Residues in Vegetables and Fruits Using LC-MS/MS.
Authors: Nakajima T, Otsuka K, Tomizawa S, Masubuchi T, Kamijo K, Yamaki Y, Yoshikawa S, Hasegawa E, Kokaji Y, Watanabe S, Hashimoto T Abstract We measured the residual amounts of chlorantraniliprole in various vegetables and fruits. Sample solutions were prepared according to our routine procedure based on the QuEChERS method and analyzed by LC-MS/MS. Performance characteristics were evaluated for 8 kinds of food samples by means of recovery tests of 5 replicates at the concentration of 10 ng/g. Recoveries and RSDs (%) ranged from 50.2 to 93.4% and from 2.1 to 9.7%, respectively. Application of this method t...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - November 16, 2018 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[Concentration of Radioactive Cesium in Domestic Foods Collected from the Japanese Market in Fiscal Years 2014-2016].
Authors: Imamura M, Nabeshi H, Tsutsumi T, Uekusa Y, Matsuda R, Maeda T, Soga K, Teshima R, Hachisuka A, Akiyama H Abstract Between fiscal years 2014 and 2016, we surveyed the concentration of radioactive cesium in commercial foods produced in areas where there is a risk of radiation contamination due to the Fukushima Daiichi nuclear disaster. The number of samples with a concentration of radioactive cesium that exceeded the regulatory limit (100 Bq/kg for general foods) was 9 out of 1,516 (0.6%) in fiscal 2014, 12 out of 900 (1.3%) in fiscal 2015, and 10 out of 654 (1.5%) in fiscal 2016. Even...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - November 16, 2018 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[Development of LC-MS and LC-MS/MS Methods for Free Asparagine in Grains].
Authors: Chikasou M, Inohana S, Yokozeki T, Tuchiya H, Fujita K Abstract New analytical methods for the determination of free asparagine (Asn), which is a precursor of acrylamide, in grains were developed using LC-MS and LC-MS/MS. Asn was extracted from a sample with 5% (w/v) aqueous trichloroacetic acid solution, appropriately diluted with 0.1% (v/v) formic acid solution, and then analyzed by LC-MS or LC-MS/MS. HPLC separation was performed by isocratic elution on a Penta Fluoro Phenyl (PFP) column using 0.1% (v/v) formic acid and acetonitrile mixture as the mobile phase. The calibration ...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - November 16, 2018 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[Major Vehicles and O-Serogroups in Foodborne Enterotoxigenic Escherichia coli Outbreaks in Japan, and Effective Detection Methods of the Pathogen in Food Associated with An Outbreak].
Authors: Konishi N, Obata H, Kai A, Ohtsuka K, Nishikawa Y, Terajima J, Hara-Kudo Y Abstract Enterotoxigenic Escherichia coli (ETEC) is a common pathogen in developing countries, and causes foodborne infections through contaminated vegetables and water. ETEC also caused some foodborne infections in developed countries, though the vehicles are often unclear. We analyzed ETEC foodborne outbreaks in Japan based on the National Food Poisoning Statistics. Vegetables and private well water accounted for 50% and 22.2% of vehicles, respectively. The main vehicles were similar to those in developing countries. Serogroups of...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - August 31, 2018 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[Determination of Hexazinone and Its Major Metabolites in Livestock Products by LC-MS/MS].
Authors: Kubota A, Okabe R, Kakimoto Y, Nemoto S, Aoyagi M Abstract A method for the determination of hexazinone and three metabolites (hexazinone metabolite B, hexazinone metabolite C, hexazinone metabolite F) in livestock products by LC-MS/MS was developed. Hexazinone and the three metabolites were extracted from a sample with acetonitrile in the presence of n-hexane, and lipid was removed by acetonitrile/n-hexane partition. The acetonitrile extract was cleaned up using a SAX/PSA cartridge column. Average recoveries (n=5) of hexazinone and the three metabolites from cattle meat, fat, liver and milk spiked at the ...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - August 31, 2018 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[A Rapid and Simple Method for Detection of Colchicum autumnale Using PCR].
Authors: Terai A, Hagino K, Asakura H, Ogai M, Yanagihara M, Kimura K, Tanaka T, Kan K, Nakamura K, Aragane M, Nakano H, Monma K, Sasamoto T Abstract Colchicum autumnale is a perennial, toxic plant that originated in Europe and North Africa. Although inedible, it is occasionally consumed accidentally because it resembles the edible Allium victorialis and other related species. This misidentification has led to episodes of food poisoning in Japan. However, determining the causative agent of a food poisoning outbreak by observing the sample visually or analyzing the chemical composition is challenging when dealing wi...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - August 31, 2018 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[Analytical Method of PCBs in Fishes by GC-MS/MS Using an Accelerated Solvent Extractor].
Authors: Otani H, Tamura Y, Baba I, Hayashi M, Morioka M, Shindo T, Hashimoto T Abstract An analytical method for PCBs in fishes using an accelerated solvent extractor (ASE) and GC-MS/MS was evaluated. After the extraction of ASE at 125℃ with n-hexane and clean-up with an AgNO3 silica gel/H2SO4 silica gel multilayer column, samples were analyzed by GC-MS/MS. This method was fast, effective and easy to operate. The limit of quantitation of the method was calculated to be 0.78 μg/kg for total PCBs. The recovery and the coefficient of variation of PCBs (n=5) from 6 fishes (Japanese sea perch, chub mackerel, yello...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - August 31, 2018 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[Inter-laboratory Study of an HPLC-UV Method for Free Asparagine in Grains].
Authors: Yokozeki T, Tsuchiya H, Fujita K Abstract In order to validate an HPLC-UV method for the determination of free asparagine, which is a precursor of acrylamide, in grains using dansyl derivatization, an inter-laboratory study was performed in 9 laboratories using 5 kinds of grains (non-glutinous brown rice flour, corn flour, strong flour, whole wheat flour, and whole rye flour), which naturally contain free asparagine. The relative standard deviations for repeatability (RSDr) and reproducibility (RSDr) were in the ranges of 0.5-2.2 and 2.3-5.9%, respectively. The HorRat values ranged from 0.4 to 0.6. These r...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - August 31, 2018 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[Nationwide Online Survey Method to Estimate Ongoing Adverse Events Caused by Supplement Use: Application to Diarrhea].
Authors: Nishijima C, Chiba T, Sato Y, Yamada H, Umegaki K Abstract The current adverse event reporting systems are inadequate to prevent potential health damage caused by dietary supplement use, and a better case-collecting system is needed. The aim of this study was to examine whether online surveys would be a useful methodology for estimating ongoing adverse events, focusing on diarrhea as a model symptom. We used four online survey companies and requested that they administer the same questionnaire to their registrants regarding experiences of adverse events associated with supplement use and details of diarrhe...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - July 25, 2018 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[Toxicity of d-Arabinose in Male and Female Rats].
Authors: Higaki S, Matsuo T Abstract In order to investigate the feasibility of using the rare sugar d-arabinose as a functional food material, we examined its toxicity in rats. In an acute toxicity study, the lethal dose (LD50) was calculated to be 12.1 g/kg in males and 11.6 g/kg in females. On the other hand, in a short-term toxicity test, rats developed diarrhea when given feed containing 5% d-arabinose, which was the minimum amount added, so the maximum nontoxic amount was estimated to be less than 5%. Thus, the toxicity of d-arabinose is stronger than that of another rare sugar, d-psicose, which was reported ...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - July 25, 2018 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[Determination of Histamine by a Rapid Fluorescamine-UPLC Method].
Authors: Murakami Y, Nakai N, Ando N, Okayama A Abstract We have developed a rapid analysis method for determination of histamine using ultra-performance liquid chromatography and fluorescamine derivatization. Histamine was extracted from foods with trichloroacetic acid after homogenization. The supernatant obtained by centrifugation was filtered and derivatized without solid-phase extraction. The mobile phase was 10 mM phosphate-acetonitrile (75 : 25) without ion-pairing. Recovery tests of histamine from fishes, seasoning and fish products gave acceptable recovery (95.8-117.7%). This method should be useful for ra...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - July 25, 2018 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[Development of a Method for Simultaneous Analysis of Egg and Milk Major Allergens in Beverage Products Using High-Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry].
Authors: Yamashita A, Miyamoto Y, Harayama K, Suzuki K Abstract A method for simultaneous analysis of egg and milk allergens using LC-QTOF-MS was developed. The proteins measured were α-casein, β-lactoglobulin, and ovalbumin, which are the main protein allergens in milk and eggs. The proteins were digested using trypsin, and the digests were analyzed by LC-QTOF-MS. Sixteen peaks were detected that confirmed the amino acid sequences of the digests, and a MRM method with high resolution (MRM-HR) using product ions of these peaks was applied for quantification. Next, validation studies were performed using ...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - July 25, 2018 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research

[PCR-RFLP Method for Rapid Discrimination of Toxic Plants Involved in Food Poisoning].
Authors: Shinozaki J, Kazuma K, Satake M, Kondo K, Konno K Abstract Food poisoning caused by natural toxins, especially poisonous plants, is characterized by severe symptoms and a relatively high mortality rate. Therefore, rapid and accurate identification of the causative agent is extremely important. From plant toxin food poisoning data published by the Ministry of Health, Labour and Welfare of Japan from 1989 to 2015, we selected five plants (Veratrum spp., Datura spp., Aconitum spp., Narcissus spp. and Colchicum spp.) that are frequently involved in poisoning outbreaks, and developed a PCR-RFLP assay to discrim...
Source: Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan - July 25, 2018 Category: Food Science Tags: Shokuhin Eiseigaku Zasshi Source Type: research