Generation of Self-Fertilizing Hermaphroditic Fish from Gonochoristic Fish, Medaka ( < b > < i > Oryzias latipes < /i > < /b > )
In this study, we attempted to generate self-fertilizing medaka from female medaka by modifying germline sex usingfoxl3−/− mutants and by using exogenous androgen to induce partial sex reversal of somatic cells.Methods:foxl3−/− XX females were treated with 11-ketotestosterone (11-KT), a potent teleost fish androgen, at the sexually mature stage for 30 days (90 –120 dph). Then, the fish were kept under normal conditions until they were either being dissected or crossed with infertile males.Results and Discussion: We showed that thefoxl3−/− XX female medaka can be transformed into a self-fertilizing hermaphrodi...
Source: Sexual Development - November 30, 2022 Category: Biology Source Type: research
Sex Determination and Ovarian Development in Reptiles and Amphibians: From Genetic Pathways to Environmental Influences
Background: Reptiles and amphibians provide untapped potential for discovering how a diversity of genetic pathways and environmental conditions are incorporated into developmental processes that can lead to similar functional outcomes. These groups display a multitude of reproductive strategies, and whereas many attributes are conserved within groups and even across vertebrates, several aspects of sexual development show considerable variation.Summary: In this review, we focus our attention on the development of the reptilian and amphibian ovary. First, we review and describe the events leading to ovarian development, incl...
Source: Sexual Development - November 15, 2022 Category: Biology Source Type: research
Subject Index
Sex Dev 2022;16:226 (Source: Sexual Development)
Source: Sexual Development - November 3, 2022 Category: Biology Source Type: research
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Sex Dev 2022;16:226 (Source: Sexual Development)
Source: Sexual Development - November 3, 2022 Category: Biology Source Type: research
Author Index
Sex Dev 2022;16:225 (Source: Sexual Development)
Source: Sexual Development - November 3, 2022 Category: Biology Source Type: research
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Sex Dev 2022;16:225 (Source: Sexual Development)
Source: Sexual Development - November 3, 2022 Category: Biology Source Type: research
Prelims
Sex Dev 2022;16:73 –76 (Source: Sexual Development)
Source: Sexual Development - November 3, 2022 Category: Biology Source Type: research
Genetics of Differences of Sex Development
Sex Dev (Source: Sexual Development)
Source: Sexual Development - September 29, 2022 Category: Biology Source Type: research
A New Understanding, Guided by Single-Cell Sequencing, of the Establishment and Maintenance of the Ovarian Reserve in Mammals
Background: Oocytes are a finite and non-renewable resource that are maintained in primordial follicle structures. The ovarian reserve is the totality of primordial follicles, present from birth, within the ovary and its establishment, size, and maintenance dictates the duration of the female reproductive lifespan. Understanding the cellular and molecular dynamics relevant to the establishment and maintenance of the reserve provides the first steps necessary for modulating both individual human and animal reproductive health as well as population dynamics.Summary: This review details the key stages of establishment and mai...
Source: Sexual Development - September 19, 2022 Category: Biology Source Type: research
Erratum
Sex Dev (Source: Sexual Development)
Source: Sexual Development - July 26, 2022 Category: Biology Source Type: research
DHX37 and 46,XY DSD: A New Ribosomopathy?
Recently, a series of recurrent missense variants in the RNA-helicaseDHX37 have been reported associated with either 46,XY gonadal dysgenesis, 46,XY testicular regression syndrome (TRS), or anorchia. All affected children have non-syndromic forms of disorders/differences of sex development (DSD). These variants, which involve highly conserved amino acids within known functional domains of the protein, are predicted by in silico tools to have a deleterious effect on helicase function. DHX37 is required for ribosome biogenesis in eukaryotes, and how these variants cause DSD is unclear. The relationship between DHX37 and huma...
Source: Sexual Development - July 14, 2022 Category: Biology Source Type: research
The Molecular Basis of 5 α-Reductase Type 2 Deficiency
The 5 α-reductase type 2 enzyme catalyzes the conversion of testosterone into dihydrotestosterone, playing a crucial role in male development. This enzyme is encoded by theSRD5A2 gene, which maps to chromosome 2 (2p23), consists of 5 exons and 4 introns, and encodes a 254 amino acid protein. Disruptions in this gene are the molecular etiology of a subgroup of differences of sex development (DSD) in 46,XY patients. Affected individuals present a large range of external genitalia undervirilization, ranging from almost typically female external genitalia to predominantly typically male external genitalia with minimal undervi...
Source: Sexual Development - July 6, 2022 Category: Biology Source Type: research
9th International Symposium in Disorders/Differences of Sex Development
See separate collated abstract fileSex Dev 2022;16:1 –91 (Source: Sexual Development)
Source: Sexual Development - June 30, 2022 Category: Biology Source Type: research
Can Non-Coding < b > < i > NR5A1 < /i > < /b > Gene Variants Explain Phenotypes of Disorders of Sex Development?
Conclusion: Evaluation of clinical and phenotypic significance of variants located in a non-coding region of a gene can be complex, and little is known regarding their association with DSD. Nevertheless, based on the important region for interaction with cofactors essential to promote appropriated sex development and on our in vitro results, it is feasible to say that an impact on gene expression can be expected and that this may be correlated with the DSD pathophysiology presented in our patients. Considering the number of cases that remain elusive after screening for the well-known DSD related genes, we emphasize the imp...
Source: Sexual Development - June 28, 2022 Category: Biology Source Type: research
SOX Genes and Their Role in Disorders of Sex Development
SOX genesare master regulatory genes controlling development and are fundamental to the establishment of sex determination in a multitude of organisms. The discovery of the master sex-determining geneSRY in 1990 was pivotal for the understanding of how testis development is initiated in mammals. With this discovery, an entire family of SOX factors were uncovered that play crucial roles in cell fate decisions during development. The importance of SOX genes in human reproductive development is evident from the various disorders of sex development (DSD) upon loss or overexpression of SOX gene function. Here, we review the rol...
Source: Sexual Development - June 27, 2022 Category: Biology Source Type: research
