Revista Brasileira de Farmacognosia This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.

Quantification of catechin in the spray-dried extract of Pimenta pseudocaryophyllus
Abstract This paper describes the quantification of catechin in the spray-dried extract of Pimenta pseudocaryophyllus (Gomes) Landrum, Myrtaceae, citral chemotype using a validated HPLC-PDA method. The method employs a RP-18 column with acetonitrile:water-orthophosphoric acid 0.05% (gradient system) and UV detection at 210 nm. The method was demonstrated to be simple, sensitive, specific, linear, precise, accurate and robust. The response was linear over a range of 5 – 200 µg/ml (r> 0.999). The range of recoveries was 92.27 – 102.54%. The relative standard deviation values for intra- and inter-day precision studies...
Source: Revista Brasileira de Farmacognosia - November 17, 2017 Category: Drugs & Pharmacology Source Type: research

Iridoids from leaf extract of Genipa americana
Abstract Genipa americana L., Rubiaceae, is a plant native from Brazil popularly known as “ jenipapo ” . Two iridoids, 1-hydroxy-7-(hydroxymethyl)-1,4aH,5H,7aH-cyclopenta[c]pyran-4-carbaldehyde (1), and iridoid 7-(hydroxymethyl)-1-methoxy-1H,4aH,5H,7aH-cyclopenta[c]pyran-4-carbaldehyde (2) were isolated and identified in the leaf extract of G. americana. Compounds 1 and 2 were identified for the first time in G. americana, and 1 has not been yet described in literature. These substances were analyzed by spectroscopic techniques such as infrared, high resolution mass spectrometry, 1H and 13C 1D; as well as 2D nuclear ma...
Source: Revista Brasileira de Farmacognosia - November 17, 2017 Category: Drugs & Pharmacology Source Type: research

Sedative and muscle relaxant activities of diterpenoids from Phlomidoschema parviflorum
Abstract Phlomidoschema parviflorum (Benth.) Vved. (Basionym: Stachys parviflora Benth.) Lamiaceae, have significance medicinal importance as it is used in number of health disorders including diarrhea, fever, sore mouth and throat, internal bleeding, weaknesses of the liver and heart genital tumors, sclerosis of the spleen, inflammatory tumors and cancerous ulcers. The present contribution deals with the sedative and muscle relaxant like effects of diterpenoids trivially named stachysrosane and stachysrosane, isolated from the ethyl acetate soluble fraction of P. parviflorum. Both compounds (at 5, 10 and 15 mg/kg, i.p) we...
Source: Revista Brasileira de Farmacognosia - November 17, 2017 Category: Drugs & Pharmacology Source Type: research

Punica granatum suppresses colon cancer through downregulation of Wnt/β-Catenin in rat model
This study aims to elucidate the beneficial effect of Punica granatum L., Lythraceae (pomegranate) peel extract in the management of colon cancer induced intrarectally with N-methylnitrosourea. Adult male Sprague-Dawley rats were administered N-methylnitrosourea (2 mg in 0.5 ml water/rat) intrarectally three times/week for five weeks to induce colorectal cancer, followed by treatment with either 5-fluorouracil (12.5 mg/kg, i.p.) or Punica peel extract (2.25 or 4.5 g/kg, p.o.). Developed tumor elevated plasma TGF- β , and Bcl2, serum epidermal growth factor, carcinoembryonic antigen, colon cancer specific antigens, and mat...
Source: Revista Brasileira de Farmacognosia - November 17, 2017 Category: Drugs & Pharmacology Source Type: research

Isovitexin as marker and bioactive compound in the antinociceptive activity of the Brazilian crude drug extracts of Echinodorus scaber and E. grandiflorus
Abstract Echinodorus scaber Rataj and Echinodorus grandiflorus (Cham.& Schltdl.) Micheli, Alismataceae, are popularly used to relieve inflammatory complaints and as diuretic. A study on the antinociceptive effect and selected marker compounds in eleven extracts from different locations was undertaken and their antinociceptive effect was assessed. The fingerprints were compared by HPLC-DAD and the content of vitexin, isovitexin, isoorientin and vitexin-2-O-rhamnoside were determined. All samples presented antinociceptive activity reducing the writhes by 36.4-62.5% and 47.4-79.8% at 10 and 50 mg/kg, respectively; indomet...
Source: Revista Brasileira de Farmacognosia - November 17, 2017 Category: Drugs & Pharmacology Source Type: research

Anti-caries activity of selected Sudanese medicinal plants with emphasis on Terminalia laxiflora
Abstract In Sudan, some medicinal plants, such as Acacia seyal, Calotropis procera and Balanites aegyptiaca have been used to prevent or treat oral health problems. The stem and stem bark of Terminalia laxiflora Engl., Combretaceae, are used as antiseptics for mouthwash to prevent gingivitis and thrush in Africa. Methanol and 50% hydroethanolic extracts of 25 plants that are used in traditional Sudanese medicine for several diseases and cavity disorders were screened for anti-cavity activities. T. laxiflora methanolic wood extracts, which exhibited such activity, were investigated. The crude extracts were assayed for their...
Source: Revista Brasileira de Farmacognosia - November 17, 2017 Category: Drugs & Pharmacology Source Type: research

Short-term carcinogenesis evaluation of Casearia sylvestris
In this study, we proposed a short-term carcinogenesis evaluation of C. sylvestris in Wistar rats, aiming to check the safety of this species to use it as proposed by Brazilian Unified Health System. C. sylvestris leaves were obtained and the crude extract was prepared by maceration from methanol/water. Wistar rats were orally treated for 12 weeks with 50, 250 or 500 mg kg − 1 of crude extract or vehicle. Body weight, daily morbidity and mortality were monitored. Blood and bone marrow samples were collect for micronucleus test, comet assay and tumor markers evaluation. Vital organs were removed to macro and histopatholog...
Source: Revista Brasileira de Farmacognosia - November 17, 2017 Category: Drugs & Pharmacology Source Type: research

Anti-adhesion potential of non-polar compounds and extracts from Ficus natalensis
Abstract Four triterpenoids, ergosta-4,6,8(14),22-tetraene-3-one 1, stigma-4-ene-3-one 2, 3 β -hydroxy-21 β -H-hop-22(29)-ene 3, sitosterol and a quinone, tectoquinone 4, were isolated from the leaf, stem bark and fruit extracts of Ficus natalensis subsp. natalensis, Moraceae, a medicinal fig found in Africa. The pure compounds 1 – 4 and crude extracts were tested for their antibacterial activity against five Gram-negative and seven Gram-positive strains and for their potential anti-biofilm activity. Antimicrobial susceptibility was observed with all pure compounds tested at 250 µg against the majority of Gram-negativ...
Source: Revista Brasileira de Farmacognosia - November 17, 2017 Category: Drugs & Pharmacology Source Type: research

Antiproliferative effects of pinostrobin and 5,6-dehydrokavain isolated from leaves of Alpinia zerumbet
This study was designed to identify the chemical constituents of hydroalcoholic and dichloromethane extracts from A. zerumbet leaves and to investigate their in vitro antiproliferative activity. The isolated phytochemicals included kaempferol, dihydro-5,6-dehydrokavain, 5,6-dehydrokavain, and pinostrobin. The hydroalcoholic extract inhibited cellular proliferation only at high concentrations, while the dichloromethane extract showed a moderate antiproliferative effect against leukemia and lung tumor cell lines. 5,6-Dehydrokavain showed potent cytostatic activity against glioblastoma cells and a moderate effect on all other...
Source: Revista Brasileira de Farmacognosia - November 17, 2017 Category: Drugs & Pharmacology Source Type: research

Antinociceptive activity of Cistanche salsa stolons, growing in the Republic of Kazakhstan
The objective of this study was to evaluate the antinociceptive effect of the hydroethanol extract of Cistanche salsa (C.A.Mey.) Beck, Orobanchaceae, stolons in animal models of pain. Chemical composition of Herba Cistanche was analyzed by HPLC-UV. Mice Swiss Webster (25 – 30 g, n = 6) were orally pre-treated with Herba Cistanche (10, 30 or 100 mg/kg) and evaluated in the formalin test and in the capsaicin- or glutamate-induced licking response. Kazakh Herba Cistanche is composed mainly by phenylpropanoid glycosides, from which echinacoside, acteoside and tubuloside B are the main constituents. When Herba Cistanche was a...
Source: Revista Brasileira de Farmacognosia - November 17, 2017 Category: Drugs & Pharmacology Source Type: research

GC–MS based metabolite profiling and angiotensin I-converting enzyme inhibitory property of black tea extracts
Abstract Angiotensin I-converting enzyme inhibitors are used as therapeutic agents for the treatment of hypertension. Regular consumption of black tea (Camellia sinensis (L.) Kuntze, Theaceae) has been reported to lower blood pressure. The aims of the present work were to compare chemical composition and angiotensin I-converting enzyme inhibitory properties of infusion and decoction of four samples of black tea. GC/MS based metabolomics approach helped in identification of fifty-one metabolites including ten organic acids, one inorganic acid, sixteen amino acids, two sugars, five sugar alcohols, fifteen phenols and flavono...
Source: Revista Brasileira de Farmacognosia - November 17, 2017 Category: Drugs & Pharmacology Source Type: research

Identification of phenolic compounds in Myricaria bracteata leaves by high-performance liquid chromatography with a diode array detector and liquid chromatography with tandem mass spectrometry
This study shows that M. bracteata leaves are a source of flavonoids with possible biological activities. (Source: Revista Brasileira de Farmacognosia)
Source: Revista Brasileira de Farmacognosia - November 17, 2017 Category: Drugs & Pharmacology Source Type: research

Gamma radiation treatment activates glucomoringin synthesis in Moringa oleifera
Abstract Plants are a very rich source of pharmacologically relevant metabolites. However, the relative concentrations of these compounds are subject to the genetic make-up, the physiological state of the plant as well as environmental effects. Recently, metabolic perturbations through the use of abiotic stressors have proven to be a valuable strategy for increasing the levels of these compounds. Oxidative stress-associated stressors, including ionizing radiation, have also been reported to induce metabolites with various biological activities in plants. Hence, the aim of the current study was to investigate the effect of ...
Source: Revista Brasileira de Farmacognosia - November 17, 2017 Category: Drugs & Pharmacology Source Type: research

Perillanolides A and B, new monoterpene glycosides from the leaves of Perilla frutescens
Abstract Two new monoterpene glycosides, perillanolides A and B, together with a known compound reported from the genus Perilla for the first time were isolated and characterized from the leaves of Perilla frutescens (L.) Britton, Lamiaceae, a garnish and colorant for foods as well as commonly used for traditional medicine. The structures of the isolated compounds were elucidated on the basis of extensive spectroscopic evidences derived from nuclear magnetic resonance experiments, mass spectrometry and by comparing their physical and spectroscopic data of literature. These compounds, together with the previously isolated s...
Source: Revista Brasileira de Farmacognosia - November 17, 2017 Category: Drugs & Pharmacology Source Type: research

Chemical identification and quantification of Hu-Gu capsule by UHPLC–Q-TOF-MS and HPLC-DAD
Abstract Hu-Gu capsule is a traditional Chinese medical formula for preventing glucocorticoid-induced osteoporosis. In this work, an ultra-high performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry was developed for chemical identification of the phytochemical compositions in positive ion mode. A total of 41 compounds were detected in Hu-Gu capsule, and 25 compounds were characterized and identified by retention time, accurate mass within 5 ppm error and characteristic fragment ions. Among of them, four major flavonoids were further quantified by high performance...
Source: Revista Brasileira de Farmacognosia - November 17, 2017 Category: Drugs & Pharmacology Source Type: research