Effect of the optimized selective enrichment medium on the expression of the p60 protein used as L. monocytogenes antigen in specific sandwich ELISA
Publication date: Available online 3 April 2019Source: Research in MicrobiologyAuthor(s): Marie-Christine Etty, Sabato D’Auria, Carole Fraschini, Stephane Salmieri, Monique LacroixAbstractThis paper presents the effects of the composition of different media (i.e., Tryptic soy broth (TSB), Brain heart infusion (BHI), Listeria enrichment broth (LEB), Fraser broth (FB) and University of Vermont medium (UVM)) on the detection of a short peptide fragment PepD specific to the p60 protein (p60) of Listeria. monocytogenes by a monoclonal antibody (anti-PepD mAb). Expression of the p60 obtained was demonstrated to be proportional...
Source: Research in Microbiology - April 5, 2019 Category: Microbiology Source Type: research
The bifunctional protein GlmU is a key factor in biofilm formation induced by alkylating stress in Mycobacterium smegmatis
Publication date: Available online 3 April 2019Source: Research in MicrobiologyAuthor(s): Angela Di Somma, Marianna Caterino, Vijay Soni, Meetu Agarwal, Pamela di Pasquale, Stefania Zanetti, Paola Molicotti, Sara Cannas, Vinay Kumar Nandicoori, Angela DuilioAbstractLiving organisms have developed specific defence mechanisms to counteract hostile environmental conditions. Alkylation stress response mechanisms also occur in Mycobacterium tuberculosis (MTB) the pathogen responsible for tuberculosis. The effect of alkylating agents on the cellular growth of MTB was investigated using methyl methanesulfonate (MMS) as methyl don...
Source: Research in Microbiology - April 3, 2019 Category: Microbiology Source Type: research
Serum level of sex steroid hormone is associated with diversity and profiles of human gut microbiome
Publication date: Available online 30 March 2019Source: Research in MicrobiologyAuthor(s): Ji-Hee Shin, Young Hee Park, Minju Sim, Seong-Ah Kim, Hyojee Joung, Dong-Mi ShinAbstractGut microbiota plays roles in host physiology including endocrine function. Although some data suggest a potential connection between biological sex differences and gut microbiota, the connection between sex steroid hormones and gut microbes remained unexplored. The current study investigates the relationship between gut microbes and serum levels of testosterone in men and estradiol in women. Fecal microbiota from a total of 57 men (n=31) and wome...
Source: Research in Microbiology - March 30, 2019 Category: Microbiology Source Type: research
Microbiome-MX 2018: Microbiota and Microbiome opportunities in Mexico, a megadiverse country
Publication date: Available online 25 March 2019Source: Research in MicrobiologyAuthor(s): Fernanda Cornejo-Granados, Ana María Calderón de la Barca, Nimbe Torres, Esperanza Martínez-Romero, Javier Torres, Yolanda López-Vidal, Xavier Soberón, Laila P. Partida-Martínez, Sandra Pinto-Cardoso, Luis David Alcaraz, Liliana Pardo-López, Samuel Canizales-Quinteros, José Luis Puente, Adrián Ochoa-LeyvaAbstractA weekly conference series paired with lectures entitled “Microbiome-MX: exploring the Microbiota and Microbiome Research in Mexico” was organized to provide a multidisciplinary overview of the most recent resear...
Source: Research in Microbiology - March 26, 2019 Category: Microbiology Source Type: research
Description of Klebsiella africanensis sp. nov., Klebsiella variicola subsp. tropicalensis subsp. nov. and Klebsiella variicola subsp. variicola subsp. nov.
Publication date: Available online 25 February 2019Source: Research in MicrobiologyAuthor(s): Carla Rodrigues, Virginie Passet, Andriniaina Rakotondrasoa, Thierno Abdoulaye Diallo, Alexis Criscuolo, Sylvain BrisseAbstractThe bacterial pathogen Klebsiella pneumoniae comprises several phylogenetic groups (Kp1 to Kp7), two of which (Kp5 and Kp7) have no taxonomic status. Here we show that group Kp5 is closely related to Klebsiella variicola (Kp3), with an average nucleotide identity (ANI) of 96.4%, and that group Kp7 has an ANI of 94.7% with Kp1 (K. pneumoniae sensu stricto). Biochemical characteristics and chromosomal beta-...
Source: Research in Microbiology - March 13, 2019 Category: Microbiology Source Type: research
Description of Klebsiella africanensis sp. nov., Klebsiella variicola subsp. tropicalensis subsp. nov. and Klebsiella variicola subsp. variicola subsp. nov
Publication date: Available online 25 February 2019Source: Research in MicrobiologyAuthor(s): Carla Rodrigues, Virginie Passet, Andriniaina Rakotondrasoa, Thierno Abdoulaye Diallo, Alexis Criscuolo, Sylvain BrisseAbstractThe bacterial pathogen Klebsiella pneumoniae comprises several phylogenetic groups (Kp1 to Kp7), two of which (Kp5 and Kp7) have no taxonomic status. Here we show that group Kp5 is closely related to Klebsiella variicola (Kp3), with an average nucleotide identity (ANI) of 96.4%, and that group Kp7 has an ANI of 94.7% with Kp1 (K. pneumoniae sensu stricto). Biochemical characteristics and chromosomal beta-l...
Source: Research in Microbiology - February 26, 2019 Category: Microbiology Source Type: research
Long-term application of nitrogen, not phosphate or potassium, significantly alters the diazotrophic community compositions and structures in a Mollisol in northeast China
This study investigated the effects of 35 years of application of inorganic fertilizers containing nitrogen (N), phosphate (P), or potassium (K) alone or in combination on the abundance and composition of diazotrophic community in a black soil (Mollisol) in northeast China. The abundance and composition of diazotrophic community were analyzed using qPCR and Illumina MiSeq sequencing targeting nifH genes. Nitrogen fertilization decreased the abundance and Shannon diversity of nifH genes. The diazotrophic community was dominated by Alphaproteobacteria (Bradyrhizobium at the genus level), with relatively higher abundance in t...
Source: Research in Microbiology - February 26, 2019 Category: Microbiology Source Type: research
The transcription factor NemR is an electrophile-sensing regulator important for the detoxification of reactive electrophiles in Acinetobacter nosocomialis
Publication date: Available online 21 February 2019Source: Research in MicrobiologyAuthor(s): Bindu Subhadra, Surya Surendran, Dong Ho Kim, Kyungho Woo, Man Hwan Oh, Chul Hee ChoiAbstractNemR is an electrophile-sensing regulator which controls two enzymes required for the detoxification of reactive electrophiles: N-ethylmaleimide (NEM) reductase and glyoxalase I in E. coli. Both enzymes are essential for bacterial survival in the presence of toxic reactive electrophiles, such as N-ethylmaleimide and methyl glyoxal. Here, we report the identification and characterization of NemR from Acinetobacter nosocomialis, a nosocomial...
Source: Research in Microbiology - February 22, 2019 Category: Microbiology Source Type: research
Editorial board
Publication date: January–February 2019Source: Research in Microbiology, Volume 170, Issue 1Author(s): (Source: Research in Microbiology)
Source: Research in Microbiology - February 8, 2019 Category: Microbiology Source Type: research
Protective and therapeutic application of the depolymerase derived from a novel KN1 genotype of Klebsiella pneumoniae bacteriophage in mice
In conclusion, the phage-encoded depolymerase Dp42 represents a potential alternative strategy for controlling infections mediated by K. pneumoniae expressing the KN1 capsular polysaccharide. (Source: Research in Microbiology)
Source: Research in Microbiology - February 2, 2019 Category: Microbiology Source Type: research
Yeast symmetric arginine methyltransferase Hsl7 has a repressive role in transcription
Publication date: Available online 17 January 2019Source: Research in MicrobiologyAuthor(s): Hong-Yeoul Ryu, Ruxin Duan, Seong Hoon AhnAbstractProtein arginine methylation, an evolutionarily conserved post-translational modification, serves critical cellular functions by transferring a methyl group to a variety of substrates, including histones and some transcription factors. In budding yeast, Hsl7 (histone synthetic lethal 7) displays type II PRMT (protein arginine methyltransferase) activity by generating symmetric dimethylarginine residues on histone H2A in vitro. However, identification of the in vivo substrate of Hsl7...
Source: Research in Microbiology - January 18, 2019 Category: Microbiology Source Type: research
LoiA directly represses lon gene expression to activate the expression of Salmonella pathogenicity island-1 genes
In this study, we showed that LoiA directly represses the expression of the lon gene during invasion by binding to the promoter region of lon. The activation of S. Typhimurium SPI-1 gene expression by LoiA is associated with its repression of lon. Moreover, through invasion and mouse infection assays, we found that the repression of lon by LoiA contributes to S. Typhimurium invasion of intestinal epithelial cells and virulence in mice. However, LoiA does not influence lon expression during S. Typhimurium growth in macrophages, which may be associated with the low expression level of loiA in macrophages. Collectively, these...
Source: Research in Microbiology - January 12, 2019 Category: Microbiology Source Type: research
A Type III Effector XopLXcc8004 is vital for Xanthomonas campestris pathovar campestris to regulate plant immunity
Publication date: Available online 27 December 2018Source: Research in MicrobiologyAuthor(s): Xia Yan, Jun Tao, Hong-Li Luo, Lei-Tao Tan, Wei Rong, Hui-Ping Li, Chao-Zu HeAbstractXanthomonas campestris pv. campestris (Xcc) secretes a suite of effectors into host plants via the type III secretion system (T3SS), modulating plant immunity defenses. Strain Xcc8004 causes black rot in brassica plants, including Arabidopsis thaliana, making it a classical model for the study of Xanthomonas pathogenesis. XopLXcc8004 was defined as a T3SS effctor (T3SE) since its homologues XopLXcv85-10 from X campestris pv. vesicatoria (Xcv85-10)...
Source: Research in Microbiology - December 27, 2018 Category: Microbiology Source Type: research
Editorial board
Publication date: December 2018Source: Research in Microbiology, Volume 169, Issue 10Author(s): (Source: Research in Microbiology)
Source: Research in Microbiology - December 4, 2018 Category: Microbiology Source Type: research
Influence of various uropathogens on crystallization of urine mineral components caused by Proteus mirabilis
Publication date: Available online 4 December 2018Source: Research in MicrobiologyAuthor(s): Agnieszka Torzewska, Katarzyna Bednarska, Antoni RóżalskiAbstractInfectious urolithiasis is a consequence of long-standing urinary tract infections with urease-positive bacteria, especially Proteus spp. However, because of the often mixed nature of urinary tract infections, in the case of urinary stones formation, several species of bacteria may be involved in the process. The purpose of the study was to determine the impact of the bacterial species: Escherichia coli, Klebsiella pneumoniae, Providencia stuartii, Pseudomonas aerug...
Source: Research in Microbiology - December 4, 2018 Category: Microbiology Source Type: research
