PDR-like ABC systems in pathogenic fungi
Publication date: Available online 25 September 2019Source: Research in MicrobiologyAuthor(s): Alexis Moreno, Atanu Banerjee, Rajendra Prasad, Pierre FalsonAbstractABC transporters of the Pleiotropic Drug Resistance (PDR) family are the main actors of antifungal resistance in pathogenic fungi. While their involvement in clinical resistant strains has been proven, their transport mechanism remains unclear. Notably, one hallmark of PDR transporters is their asymmetry, with one canonical nucleotide-binding site capable of ATP hydrolysis while the other site is not. Recent publications reviewed here show that the so-called “...
Source: Research in Microbiology - September 26, 2019 Category: Microbiology Source Type: research
Simple or complex organic substrates inhibit arsenite oxidation and aioA gene expression in two β-Proteobacteria strains
Publication date: Available online 25 September 2019Source: Research in MicrobiologyAuthor(s): Tiffanie Lescure, Catherine Joulian, Clément Charles, Taoikal Ben Ali Saanda, Mickael Charron, Dominique Breeze, Pascale Bauda, Fabienne Battaglia-BrunetAbstractMicrobial transformation of arsenic species and their interaction with the carbon cycle play a major role in the mobility of this toxic metalloid in the environment. The influence of simple or complex organic substrates on arsenic bio-oxidation was studied using two bacterial strains: one – the arsenivorans strain of Thiomonas delicata – is able to use AsIII as sole ...
Source: Research in Microbiology - September 26, 2019 Category: Microbiology Source Type: research
An integrated transport mechanism of the maltose ABC importer
Publication date: Available online 24 September 2019Source: Research in MicrobiologyAuthor(s): Rebecca Mächtel, Alessandra Narducci, Douglas A. Griffith, Thorben Cordes, Cédric OrelleAbstractATP-binding cassette (ABC) transporters use the energy of ATP hydrolysis to transport a large diversity of molecules actively across biological membranes. A combination of biochemical, biophysical, and structural studies has established the maltose transporter MalFGK2 as one of the best-characterized proteins of the ABC family. MalF and MalG are the transmembrane domains, and two MalKs form a homodimer of nucleotide-binding domains. ...
Source: Research in Microbiology - September 26, 2019 Category: Microbiology Source Type: research
The extremely halophilic archaeon Halobacterium salinarum ETD5 from the solar saltern of Sfax (Tunisia) produces multiple halocins
Publication date: Available online 24 September 2019Source: Research in MicrobiologyAuthor(s): Fadoua Ghanmi, Alyssa Carré-Mlouka, Zied Zarai, Hafedh Mejdoub, Jean Peduzzi, Sami Maalej, Sylvie RebuffatAbstractThe extremely halophilic archaeon Halobacterium salinarum strain ETD5 was previously isolated from the solar saltern of Sfax (Tunisia) and shown to encode and express halocin S8. The Hbt. salinarum ETD5 culture supernatant was shown here to exhibit high antimicrobial activity against several halophilic archaea and bacteria of different genera, showing a cross-domain inhibition. The antimicrobial activity was destroye...
Source: Research in Microbiology - September 26, 2019 Category: Microbiology Source Type: research
The SUF system: an ABC ATPase-dependent protein complex with a role in Fe–S cluster biogenesis
Publication date: Available online 14 August 2019Source: Research in MicrobiologyAuthor(s): Pierre Simon Garcia, Simonetta Gribaldo, Béatrice Py, Frédéric BarrasAbstractIron-sulfur (Fe–S) clusters are considered one of the most ancient and versatile inorganic cofactors present in the three domains of life. Fe–S clusters can act as redox sensors or catalysts and are found to be used by a large number of functional and structurally diverse proteins. Here, we cover current knowledge of the SUF multiprotein machinery that synthesizes and inserts Fe–S clusters into proteins. Specific focus is put on the ABC ATPase SufC...
Source: Research in Microbiology - August 29, 2019 Category: Microbiology Source Type: research
FLO8 deletion leads to azole resistance by upregulating CDR1 and CDR2 in Candida albicans
Publication date: Available online 23 August 2019Source: Research in MicrobiologyAuthor(s): L.I. Wen-Jing, L.I.U. Jin-Yan, Ce SHI, Yue ZHAO, Ling-ning MENG, Fang Wu, X.I.A.N.G. Ming-JieAbstractCandida albicans has the ability to switch reversibly between budding yeast, filamentous, pseudohypha, and hyphal forms, a process in which the transcription factor Flo8 plays an important role. This ability is important for the virulence and pathogenicity of Candida albicans. To determine whether Flo8 plays a role in the regulation of drug sensitivity, we constructed a FLO8 null mutant flo8/flo8 from the parental strain SN152 and a ...
Source: Research in Microbiology - August 24, 2019 Category: Microbiology Source Type: research
Energy coupling in ABC exporters
Publication date: Available online 20 August 2019Source: Research in MicrobiologyAuthor(s): Hendrik W. van Veen, Himansha Singh, Kelvin Agboh, Lisa A. Fagg, Dawei Guo, Brendan Swain, Robbin F. de Kruijf, Charlotte GuffickAbstractMultidrug transporters are important and interesting molecular machines that extrude a wide variety of cytotoxic drugs from target cells. This review summarizes novel insights in the energetics and mechanisms of bacterial ATP-binding cassette multidrug transporters as well as recent advances connecting multidrug transport to ion and lipid translocation processes in other membrane proteins. (Source:...
Source: Research in Microbiology - August 20, 2019 Category: Microbiology Source Type: research
Rise and rise of the abc transporter families
Publication date: Available online 20 August 2019Source: Research in MicrobiologyAuthor(s): I.Barry Holland (Source: Research in Microbiology)
Source: Research in Microbiology - August 20, 2019 Category: Microbiology Source Type: research
The repeated 36 amino acid motif of Chlamydia trachomatis Hc2 protein binds to the major groove of DNA
Publication date: Available online 13 August 2019Source: Research in MicrobiologyAuthor(s): Odete Sofia Lopes Gonçalves, Gunna Christiansen, Arne Holm, Bjørn Herrmann, Markus Klintstedt, Steffen B. Petersen, Svend BirkelundAbstractThe gram-negative, obligate intracellular human pathogen, Chlamydia trachomatis has a bi-phasic developmental cycle. The histone H1-like C. trachomatis DNA binding protein, Hc2, is produced late during the developmental cycle when the dividing reticulate body transforms into the smaller, metabolically inactive elementary body. Together with Hc1, the two proteins compact the chlamydial chromosom...
Source: Research in Microbiology - August 14, 2019 Category: Microbiology Source Type: research
The SUF system: An ABC ATPase-dependent protein complex with a role in Fe-S cluster biogenesis
Publication date: Available online 14 August 2019Source: Research in MicrobiologyAuthor(s): Pierre Simon Garcia, Simonetta Gribaldo, Béatrice Py, Frédéric BarrasAbstractIron-sulfur (Fe-S) clusters are considered one of the most ancient and versatile inorganic cofactors present in the three domains of life. Fe-S clusters can act as redox sensors or catalysts and are found to be used by a large number of functional and structurally diverse proteins. Here, we cover current knowledge of the SUF multiprotein machinery that synthesizes and inserts Fe-S clusters into proteins. Specific focus is put on the ABC ATPase SufC, whic...
Source: Research in Microbiology - August 14, 2019 Category: Microbiology Source Type: research
Multifaceted ABC transporters associated to microcin and bacteriocin export
Publication date: Available online 8 August 2019Source: Research in MicrobiologyAuthor(s): Konstantinos Beis, Sylvie RebuffatAbstractMicrocins and bacteriocins are ribosomally-synthesized defence peptides produced by Gram-negative and -positive bacteria to target competitors in their niche. Some of them carry posttranslational modifications established by dedicated enzymes. To protect themselves from their own toxic peptides, bacteria use dedicated immunity proteins or expel the toxin using ATP-binding cassette (ABC) transporters. In this last case, this immunity function is associated to export of the antimicrobial peptid...
Source: Research in Microbiology - August 9, 2019 Category: Microbiology Source Type: research
The oligopeptide ABC-importers are essential communication channels in Gram-positive bacteria
Publication date: Available online 1 August 2019Source: Research in MicrobiologyAuthor(s): Leyla Slamti, Didier LereclusAbstractThe transport of peptides in microorganisms plays an important role in their physiology and behavior, both as a nutrient source and as a proxy to sense their environment. This latter function is evidenced in Gram-positive bacteria where cell-cell communication is mediated by small peptides. Here, we highlight the importance of the oligopeptide permease (Opp) systems in the various major processes controlled by signaling peptides, such as sporulation, virulence and conjugation. We underline that th...
Source: Research in Microbiology - August 1, 2019 Category: Microbiology Source Type: research
Roles of FtsEX in cell division
Publication date: Available online 1 August 2019Source: Research in MicrobiologyAuthor(s): Sebastien Pichoff, Shishen Du, Joe LutkenhausAbstractFtsEX is a member of a small subclass of ABC transporters that uses mechano-transmission to perform work in the periplasm. FtsEX controls periplasmic peptidoglycan (PG) hydrolase activities in many Gram negative and positive organisms to ensure the safe separation of daughter cells during division. FtsEX localizes to the Z ring and uses its ATPase activity to regulate its periplasmic effectors. In E. coli, FtsEX also participates in building the divisome and coordinates PG synthesi...
Source: Research in Microbiology - August 1, 2019 Category: Microbiology Source Type: research
The Lpt ABC transporter for lipopolysaccharide export to the cell surface
Publication date: Available online 1 August 2019Source: Research in MicrobiologyAuthor(s): Paola Sperandeo, Alessandra M. Martorana, Alessandra PolissiAbstractThe surface of the outer membrane of Gram-negative bacteria is covered by a tightly packed layer of lipopolysaccharide molecules which provide a barrier against many toxic compounds and antibiotics. Lipopolysaccharide, synthesized in the cytoplasm, is assembled in the periplasmic leaflet of the inner membrane where the intermembrane Lpt system mediates its transport to the cell surface. The first step of lipopolysaccharide transport is its extraction from the outer l...
Source: Research in Microbiology - August 1, 2019 Category: Microbiology Source Type: research
Editorial board
Publication date: June–August 2019Source: Research in Microbiology, Volume 170, Issues 4–5Author(s): (Source: Research in Microbiology)
Source: Research in Microbiology - July 19, 2019 Category: Microbiology Source Type: research
