Contemporary quality control in the IVF laboratory: heading to the cloud?
Publication date: Available online 10 May 2019Source: Reproductive BioMedicine OnlineAuthor(s): David K. Gardner, Thomas B. Pool (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - May 10, 2019 Category: Reproduction Medicine Source Type: research

Inheritance of imbalances in recurrent chromosomal translocation t(11;22): clarification by PGT-SR and sperm-FISH analysis
ConclusionsThese findings highlight the divergence that may exist between meiotic segregation and post-zygotic selection. Post-zygotic selection would be responsible for the elimination of unbalanced embryos derived from the adjacent-1 segregation mode. The combined action of several factors occurs at the beginning of post-zygotic selection. Genetic counselling must consider the risk of a birth related to the adjacent-1 segregation mode, irrespective of the sex of the translocation carrier. These results will allow deeper understanding of the PGT results of t(11;22) carriers, which often include a high number of aneuploid ...
Source: Reproductive BioMedicine Online - May 9, 2019 Category: Reproduction Medicine Source Type: research

The effect of a GnRH antagonist on follicle maturation in normal women
Publication date: Available online 11 March 2019Source: Reproductive BioMedicine OnlineAuthor(s): Serafeim Pousias, Christina I. Messini, George Anifandis, George Sveronis, Panagiotis Georgoulias, Alexandros Daponte, Ioannis E. Messinis, Konstantinos DafopoulosAbstractResearch questionGanirelix is a gonadotrophin-releasing hormone (GnRH) antagonist used for the prevention of premature LH surge during ovarian stimulation. What is the impact of ganirelix on follicle maturation in normal women?DesignTen normally cycling women were investigated during two menstrual cycles, i.e. cycle 1 (control) and cycle 2 (ganirelix). During...
Source: Reproductive BioMedicine Online - May 8, 2019 Category: Reproduction Medicine Source Type: research

Fertility of women with cystic fibrosis: a French survey
Publication date: Available online 7 May 2019Source: Reproductive BioMedicine OnlineAuthor(s): Anna Tournier, Marlène Murris, Anne Prevotat, Annlyse Fanton, Célia Bettiol, Jean ParinaudAbstractEven though the impact of cystic fibrosis (CF) on male fertility is well known, very few studies have investigated its effect on women fertility. In order to evaluate the fertility status of women with cystic fibrosis a questionnaire was sent to 220 women with cystic fibrosis. The questions concerned their desire to become a parent, achievement or not of a pregnancy, the time to became pregnant, the way to obtain pregna...
Source: Reproductive BioMedicine Online - May 8, 2019 Category: Reproduction Medicine Source Type: research

Assisted reproductive techniques in latin america: the latin american registry, 2016
ConclusionsThe number of initiated cycles continue to increase. Compared with previous years, the number of embryos transferred decreased while the proportion of single embryo transfers increased with a drop in multiple births. It is mandatory to stimulate health care providers and consumers to continue in this trend. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - May 8, 2019 Category: Reproduction Medicine Source Type: research

Weekly intramuscular progesterone for luteal phase support in women receiving oocyte donation is associated with a decreased miscarriage rate
ConclusionsAdding IM to vaginal progesterone administration appears to decrease the miscarriage rate and increase the live birth rate in oocyte donations. The initiation of IM progesterone is most beneficial when it is introduced the evening of embryo transfer. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - May 8, 2019 Category: Reproduction Medicine Source Type: research

Predictors of Reproductive Outcomes Following Myomectomy for Intramural Fibroids
Conclusion(s)Age and race play a significant role in the reproductive outcomes of infertile women undergoing IM fibroid myomectomy as part of fertility enhancement treatment. Further large prospective studies are needed to identify specific factors associated with achieving pregnancy, which will aid in determining the clinical management of infertile women with IM fibroids. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - May 7, 2019 Category: Reproduction Medicine Source Type: research

Circulatory Metastin/Kisspeptin-1 (META KISS-1 Study) in Polycystic Ovary Syndrome: A Systematic Review and Meta-analysis with Diagnostic Test Accuracy
Publication date: Available online 2 May 2019Source: Reproductive BioMedicine OnlineAuthor(s): Varikasuvu Seshadri Reddy, V. Satya Prasad, VC Vamshika, MV Satyanarayana, Panga Jaipal ReddyABSTRACTA close association of Kisspeptin-1 (KISS-1) with reproductive physiology has been reported in the literature, but the results on circulatory KISS-1 are ambiguous in patients with polycystic ovary syndrome (PCOS). We aimed to perform a systematic review and conduct a meta-analysis to evaluate the association of KISS-1 with PCOS and to test its diagnostic test accuracy (DTA) through DTA meta-analysis. Relevant studies were identifi...
Source: Reproductive BioMedicine Online - May 2, 2019 Category: Reproduction Medicine Source Type: research

Pregnancy following diagnosis of premature ovarian insufficiency: a systematic review
In conclusion regarding fertility in POI patients, no treatment has thus far shown its superiority. However, the recent advances in options such as IVM and stem cell therapy are likely to be the future of treatment and may generate new hope for these patients. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - May 2, 2019 Category: Reproduction Medicine Source Type: research

Does stress affect IVF outcomes? A prospective study of physiological and psychological stress in women undergoing IVF
ConclusionIt can be cautiously concluded that physiological and psychological stress do not negatively affect IVF outcomes. Moreover, high follicular cortisol concentrations might have positive effects on pregnancy rates. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - May 1, 2019 Category: Reproduction Medicine Source Type: research

Differential impacts of gonadotropins, IVF and embryo culture on mouse blastocyst development
This study examined developmental characteristics of mouse blastocysts derived from ART compared with in vivo-conceived embryos. To determine the effect of ovarian stimulation versus in vitro fertilization (IVF) versus in vitro embryo culture on phenotype, six distinct groups of blastocysts were generated. Female mice were naturally cycling or treated with high or mild doses of gonadotropin; followed by natural mating or IVF in clinical conditions. Embryo morphokinetics were assessed by continuous time-lapse monitoring. Cell lineage allocation to inner cell mass (Oct4+) or trophectoderm (Cdx2+) was determined by immunohist...
Source: Reproductive BioMedicine Online - May 1, 2019 Category: Reproduction Medicine Source Type: research

PGT for aneuploidy does not affect three-cycle cumulative IVF discontinuation rate in women of advanced maternal age
Publication date: Available online 12 March 2019Source: Reproductive BioMedicine OnlineAuthor(s): Sezcan Mumusoglu, Irem YaralI Ozbek, Zuhal Yapici Coskun, Mehtap Polat, Lale Karakoc sokmensuer, Gurkan Bozdag, Hakan YaraliAbstractResearch questionDoes preimplantation genetic testing for aneuploidy (PGT-A) influence the discontinuation rate in women with advanced maternal age (AMA) undergoing IVF?DesignRetrospective longitudinal cohort study carried out at a single IVF clinic in Turkey. In total, 401 consecutive AMA cases were included. Discontinuation rates of pre-intervention (conventional IVF; June 2013 to October 2014; ...
Source: Reproductive BioMedicine Online - April 28, 2019 Category: Reproduction Medicine Source Type: research

Pregnancy and neonatal complications in women with polycystic ovary syndrome in relation to second-trimester anti-Müllerian hormone levels
Publication date: Available online 27 February 2019Source: Reproductive BioMedicine OnlineAuthor(s): Ragnheidur Valdimarsdottir, Heiddis Valgeirsdottir, Anna-Karin Wikström, Theodora Kunovac Kallak, Evangelia Elenis, Ove Axelsson, Kumari Ubhayasekhera, Jonas Bergquist, Terhi T Piltonen, Pascal Pigny, Paolo Giacobini, Inger Sundström PoromaaAbstractResearch questionAn association has been found between high anti-Müllerian hormone (AMH) levels during pregnancy and the development of polycystic ovary syndrome (PCOS)-like phenotypic traits in mouse offspring. The aim of this study was to determine whether AMH le...
Source: Reproductive BioMedicine Online - April 26, 2019 Category: Reproduction Medicine Source Type: research

The obstructive interval predicts pregnancy rates in post-vasectomy patients undergoing ICSI with surgical sperm retrieval
ConclusionsMen undertaking vasectomy should be made aware of the long-term effects and their implications for future reproductive treatment. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - April 26, 2019 Category: Reproduction Medicine Source Type: research

Supraphysiologic serum estradiol impacts oocytes and is negatively related to birth weight in full term singletons born after frozen embryo transfer: a retrospective cohort study
ConclusionsPeak serum E2 during COH is negatively associated with birth weight. This model proposes a novel concept as well as new evidence that the effect on birth weight is due to the primary influence of high E2 on oocytes during COH. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - April 26, 2019 Category: Reproduction Medicine Source Type: research

The role of Cabergoline and post-collection Gonadotropin Releasing Hormone – antagonist administration for Ovarian Hyperstimulation Syndrome prevention: A Retrospective Cohort Study
ConclusionThe addition of a Cabergoline to GnRH-agonist triggering in high-risk OHSS patients and subsequently the addition of GnRH-antagonist for five days in the luteal phase, sequentially reduces the risk of mild and moderate OHSS and improves patient comfort compared to GnRH-agonist trigger alone. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - April 26, 2019 Category: Reproduction Medicine Source Type: research

Advanced Fibroid Study; paying homage to John Sampson
Publication date: Available online 25 April 2019Source: Reproductive BioMedicine OnlineAuthor(s): John L Yovich, Philip K Rowlands, Sunthra Lingam, Mark Sillender, Shanthi SrinivasanAbstractA recent article supports our long-standing view that all intramural fibroids can cause disturbance of uterine function. This may be reflected in the symptom of menorrhagia or fertility-related issues as well as pregnancy losses at all gestational stages. However, it was disappointing that there was no reference to either the mechanism by which fibroids disturb uterine function nor to the gynaecologist who described this more than 100 y...
Source: Reproductive BioMedicine Online - April 26, 2019 Category: Reproduction Medicine Source Type: research

GLP-1 receptor agonists versus metformin in polycystic ovary syndrome: a systematic review and meta-analysis
Publication date: Available online 25 April 2019Source: Reproductive BioMedicine OnlineAuthor(s): Yi Han, Yingjie Li, Bing HeABSTRACTThis meta-analysis aimed to evaluate the efficacy and safety of a GLP-1 receptor agonist on polycystic ovary syndrome (PCOS) patients by comparing its effect with that of metformin. Electronic databases dating from their establishment to June 2018 were searched to find all randomized controlled trials (RCTs) reporting the efficacy of GLP-1 receptor agonists versus metformin for patients with PCOS. Therapeutic parameters included menstrual cycle, sex hormone and clinical manifestations, glucos...
Source: Reproductive BioMedicine Online - April 26, 2019 Category: Reproduction Medicine Source Type: research

Treatment of hydrosalpinx in relation to IVF outcome: a systematic review and meta-analysis
Publication date: Available online 25 April 2019Source: Reproductive BioMedicine OnlineAuthor(s): Alexander Volodarsky-Perel, William Buckett, Togas TulandiAbstractSince the birth of the first “test tube” baby 40 years ago, the treatment of hydrosalpinx has undergone some significant changes. Salpingectomy is the most widely used treatment of hydrosalpinx. However, the effect of salpingectomy on the stimulation response during subsequent IVF treatment remains unclear. The aim of this systematic review was to evaluate the ovarian response and pregnancy outcome of IVF treatment performed after salpingectomy compa...
Source: Reproductive BioMedicine Online - April 26, 2019 Category: Reproduction Medicine Source Type: research

Association of gene polymorphisms in Anti-Müllerian hormone signaling pathway with ovarian function: a systematic review and meta-analysis
Publication date: Available online 26 April 2019Source: Reproductive BioMedicine OnlineAuthor(s): Ran Cheng, Wei Xiong, Xiaoyan Luo, Yaxian Ma, Ying Nie, Xiaoyong Qiao, Hui Chen, Ying Hu, Liangzhi XuAbstractThe systematic review evaluated whether single nucleotide polymorphisms of AMH and AMHRII genes are associated with ovarian function. We searched literatures in PubMed and Embase complemented by hand search in the reference lists. Eight studies involving 3,155 participants were included in meta-analysis and 10 studies included for description. For AMH c.146T>G polymorphism, no significant difference in serum AMH leve...
Source: Reproductive BioMedicine Online - April 26, 2019 Category: Reproduction Medicine Source Type: research

Management of thin endometrium in assisted reproduction: a clinical practice guideline from the Canadian Fertility and Andrology Society (CFAS)
The objective of this CFAS guideline is to provide evidence-based recommendations using the GRADE (Grading of Recommendations, Assessment, Development and Evaluations) framework on the assessment, impact and management of thin endometrium in assisted reproduction. The effect of endometrial thickness on pregnancy and live birth outcomes in ovarian stimulation and IVF (fresh and frozen cycles) is addressed. In addition, recommendations on the use of adjuvants to improve endometrial thickness and pregnancy outcomes are provided. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - April 25, 2019 Category: Reproduction Medicine Source Type: research

Does the Akt pathway play a role in follicle activation after grafting of human ovarian tissue?
ConclusionsThis study confirms that primordial follicle activation is an early event after transplantation, and significant follicle death also contributes to the burnout effect, eventually resulting in early depletion of the ovarian reserve. Increased Akt phosphorylation on day 3 post-grafting suggests it plays a role in follicle activation and subsequent burnout. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - April 25, 2019 Category: Reproduction Medicine Source Type: research

Comparison and appraisal of (inter)national recurrent pregnancy loss guidelines
Publication date: Available online 25 April 2019Source: Reproductive BioMedicine OnlineAuthor(s): Angelos Youssef, Nathalie Vermeulen, E. E. Lisa O. Lashley, Mariëtte Goddijn, Marie Louise P. van der HoornAbstractDiagnostic investigations and treatment options of recurrent pregnancy loss (RPL) may differ locally and internationally. This manuscript reviews the similarities and differences between existing (inter)national guidelines on RPL. The European Society of Human Reproduction and Embryology (ESHRE) guideline (2017), the American Society for Reproductive Medicine (ASRM) Committee Opinions (2013) and the Royal Col...
Source: Reproductive BioMedicine Online - April 25, 2019 Category: Reproduction Medicine Source Type: research

Live birth rates remain stable in modified natural IVF despite low AMH: analysis of 638 cycles
Publication date: Available online 25 April 2019Source: Reproductive BioMedicine OnlineAuthor(s): Lise Preaubert, Talya Shaulov, Simon Phillips, Melissa Stutz, Isaac Jacques Kadoch, Camille Sylvestre, Pierre LehmannAbstractResearch questionDo live birth rates (LBR) following modified natural IVF (mnIVF) differ according to serum AMH level?DesignRetrospective cohort study including 638 women aged ≤ 39 years starting their first mnIVF cycle at a university-affiliated private IVF center. Patients were divided into three groups, by level of AMH: ≤ 0.5 ng/ml (25th percentile), 0.51-2.03 ng/ml (25-75th, reference), and 2.0...
Source: Reproductive BioMedicine Online - April 25, 2019 Category: Reproduction Medicine Source Type: research

Is it too early to deny resveratrol supplementation in embryo transfer cycles?
Publication date: Available online 25 April 2019Source: Reproductive BioMedicine OnlineAuthor(s): Jialyu Huang, Yanping Kuang (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - April 25, 2019 Category: Reproduction Medicine Source Type: research

Pregnancy and perinatal outcomes of crown−rump length discordance in the first trimester−a retrospective cohort study of dichorionic twin pregnancies after in vitro fertilization
Conclusion(s)Discordant twin pregnancies were at increased risk for one fetal loss prior to 12 weeks’ gestation. Except for birth weight discordance, there was no significant difference between CRL discordance and other adverse perinatal outcomes. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - April 25, 2019 Category: Reproduction Medicine Source Type: research

Vaginal microbiota and IVF outcomes: poor diagnosis results in flawed conclusions
Publication date: Available online 23 April 2019Source: Reproductive BioMedicine OnlineAuthor(s): Thor Haahr, Jørgen Skov Jensen, Peter Humaidan (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - April 25, 2019 Category: Reproduction Medicine Source Type: research

Early prediction of the success of methotrexate treatment success by 24–hour pretreatment increment in HCG and day 1–4 change in HCG
This study aimed to re-evaluate the role of these markers in the earlier identification of treatment success in a large cohort of women.DesignThis was a retrospective cohort study including women diagnosed with an ectopic pregnancy and treated with a regimen of a single dose of MTX. A comparison of maternal and gestation characteristics was made between groups in whom treatment was successful or failed.ResultsA total of 292 women treated with single-dose intramuscular MTX for ectopic pregnancy were included in this study. In the overall cohort, the treatment success rate with a single dose of MTX was 62.7% (183/292). Only ...
Source: Reproductive BioMedicine Online - April 23, 2019 Category: Reproduction Medicine Source Type: research

Assessment of reflectance confocal microscopy for non-invasive selection of optimal ovarian cortex fragments for autotransplantation
ConclusionIn this proof of concept study, we have shown that RCM is a promising technique to determine the density of follicles ex vivo in living human ovarian cortex fragments, apparently without compromising the vitality of the tissue. Safety studies and further optimization of the RCM technique with a focus on increasing the penetration depth are required before clinical use of RCM. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - April 19, 2019 Category: Reproduction Medicine Source Type: research

Early versus delayed follow-up after misoprostol treatment for early pregnancy loss
Publication date: Available online 12 March 2019Source: Reproductive BioMedicine OnlineAuthor(s): Yossi Mizrachi, Liliya Tamayev, Ofer Shemer, Ilia Kleiner, Jacob Bar, Ron SagivAbstractResearch questionDoes extending the follow-up after misoprostol treatment for early pregnancy loss increase the success rate?DesignPatients who had experienced early pregnancy loss (
Source: Reproductive BioMedicine Online - April 19, 2019 Category: Reproduction Medicine Source Type: research

Editorial Board
Publication date: May 2019Source: Reproductive BioMedicine Online, Volume 38, Issue 5Author(s): (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - April 19, 2019 Category: Reproduction Medicine Source Type: research

Contents
Publication date: May 2019Source: Reproductive BioMedicine Online, Volume 38, Issue 5Author(s): (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - April 19, 2019 Category: Reproduction Medicine Source Type: research

Laser technology in the ART laboratory: a narrative review
Publication date: May 2019Source: Reproductive BioMedicine Online, Volume 38, Issue 5Author(s): Lien M. Davidson, Yaqiong Liu, Tracey Griffiths, Celine Jones, Kevin CowardAbstractTo improve success rates, assisted reproductive technology (ART) procedures continually undergo optimization and enhancement such that the best quality gametes and embryos can be identified and manipulated, thus improving clinical outcomes. Laser technology is now being applied across ART to reduce procedure times and increase the consistency and reproducibility of traditional ART techniques such as assisted hatching, embryo biopsy, intracytoplasm...
Source: Reproductive BioMedicine Online - April 19, 2019 Category: Reproduction Medicine Source Type: research

Early life environment influences the trajectory of post-partum weight loss in adult female rats
Publication date: May 2019Source: Reproductive BioMedicine Online, Volume 38, Issue 5Author(s): C.E. Aiken, J.L. Tarry-Adkins, T.J. Ashmore, S.E. OzanneAbstractResearch questionThe physiological processes of pregnancy and lactation require profound changes in maternal metabolism and energy balance. The timescale of metabolic reversion after pregnancy, in particular post-partum weight loss, is highly variable between individuals. Currently, mechanisms influencing post-partum metabolic recovery are not well understood. The hypothesis tested here is that, in common with other metabolic and obesity-related outcomes, capacity f...
Source: Reproductive BioMedicine Online - April 19, 2019 Category: Reproduction Medicine Source Type: research

Management of thin endometrium in assisted reproduction
The objective of this guideline is to provide evidence-based recommendations using the GRADE (Grading of Recommendations, Assessment, Development and Evaluations) framework on the assessment, impact and management of thin endometrium in assisted reproduction. The effect of endometrial thickness on pregnancy and livebirth outcomes in ovarian stimulation and IVF (fresh and frozen cycles) is addressed. In addition, recommendations on the use of adjuvants to improve endometrial thickness and pregnancy outcomes are provided. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - April 14, 2019 Category: Reproduction Medicine Source Type: research

The window is wide: flexible timing for frozen-thawed embryo transfer in natural cycles
ConclusionsOur results suggest that the timing of blastocyst transfer in a natural cycle after LH surge is flexible within 24 hours. Outcomes are equally good with day of embryo transfer six or seven days after LH surge date. E2 and P dynamics surrounding the date of surge did not have a significant impact on OPR. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - April 13, 2019 Category: Reproduction Medicine Source Type: research

Blood anti-müllerian hormone is a possible determinant of recurrent early miscarriage, yet not conclusive in predicting a further miscarriage
Publication date: Available online 12 April 2019Source: Reproductive BioMedicine OnlineAuthor(s): Estelle Leclercq, Luc De Saint Martin, Caroline Bohec, Marie Thérèse Le Martelot, Sylvie Roche, Zarrin Alavi, Dominique Mottier, Elisabeth PasquierAbstractResearch QuestionIs blood anti-müllerian hormone (AMH) level a strong determinant of unexplained recurrent early miscarriage?DesignIn the first part of the study, AMH levels measured using the ELISA kit of Immunotech* (Beckman-Coutler, France) were compared between 188 all-comer (mostly fertile) women consecutively referred for ≥ 3 miscarriages in the f...
Source: Reproductive BioMedicine Online - April 13, 2019 Category: Reproduction Medicine Source Type: research

Building an evidence base for IVF ‘add-ons’
Publication date: Available online 10 April 2019Source: Reproductive BioMedicine OnlineAuthor(s): NS Macklon, KK Ahuja, BCJM Fauser (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - April 11, 2019 Category: Reproduction Medicine Source Type: research

Anti-Müllerian Hormone as a Diagnostic Tool for Polycystic Ovary Syndrome Under Different Diagnostic Criteria Among Unselected Population
ConclusionAMH has poor to fair validity to diagnose PCOS among an unselected group of women, except for patients bearing all features of the syndrome (Phenotype A). This finding is valid using the NIH, Rotterdam and AE-PCOS Society criteria and even after revising the definition of PCOM as AFC≥20. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - April 11, 2019 Category: Reproduction Medicine Source Type: research

Systematic and standardized hysteroscopic endometrial injury for treatment of recurrent implantation failure
ConclusionThis study suggests that endometrial injury is beneficial in recurrent implantation failure patients to increase the odds of clinical pregnancy rates, live birth rates, and implantation rates. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - April 11, 2019 Category: Reproduction Medicine Source Type: research

Effects of hyperhomocysteinaemia and metabolic syndrome on reproduction in women with polycystic ovary syndrome: a secondary analysis
Publication date: Available online 18 January 2019Source: Reproductive BioMedicine OnlineAuthor(s): Hui Chang, Liangzhen Xie, Hang Ge, Qi Wu, Yan Wen, Duojia Zhang, Yuehui Zhang, Hongli Ma, Jingshu Gao, Chi Chiu Wang, Elisabet Stener-Victorin, Ernest HY Ng, Xiaoke WuAbstractResearch questionWhat is the association between hyperhomocysteinaemia (HHCY), metabolic syndrome, and reproductive outcomes among women with polycystic ovary syndrome (PCOS).DesignA secondary analysis of PCOSAct with 21 sites in China. A total of 1000 women with PCOS were enrolled; 936 women with baseline homocysteine (HCY) were analysed.ResultsHigher ...
Source: Reproductive BioMedicine Online - April 6, 2019 Category: Reproduction Medicine Source Type: research

Mutational analysis of theFAM175A gene in patients with premature ovarian insufficiency
ConclusionOur results suggest that the p.L243V variant of FAM175A may not be causative for POI. The contribution of FAM175A to POI needs further exploration. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - April 6, 2019 Category: Reproduction Medicine Source Type: research

Effect of recombinant human vascular endothelial growth factor on testis tissue xenotransplants from prepubertal boys: a three-case study
Publication date: Available online 12 March 2019Source: Reproductive BioMedicine OnlineAuthor(s): Elissavet Ntemou, Prashant Kadam, Sven Van Laere, Dorien Van Saen, Elena Vicini, Ellen GoossensAbstractResearch questionDoes recombinant human vascular endothelial growth factor (VEGF-165) improve the efficiency of human immature testis tissue (ITT) xenotransplantation?DesignITT fragments from three prepubertal boys were cultured for 5 days with VEGF-165 or without (control) before xenotransplantation into the testes of immunodeficient mice. Xenotransplants were recovered at 4 and 9 months after transplantation, and vasculariz...
Source: Reproductive BioMedicine Online - April 6, 2019 Category: Reproduction Medicine Source Type: research

Determinants of gestational surrogates’ satisfaction in relation to the characteristics of surrogacy cases
This study evaluated the retrospective experiences of gestational surrogates within a Canadian context. The characteristics of surrogacy arrangements carried out by the participants, factors influencing their surrogacy satisfaction, impact of surrogacy on their family functioning, and their attitudes toward carrying a pregnancy for different types of intended parents were analyzed.DesignData were collected using an anonymous online survey from 06/2016 to 02/2017 with participants recruited from the Canadian surrogacy community and the Internet.ResultsA total of 184 gestational surrogates (mean age at surrogacy: 32, range: ...
Source: Reproductive BioMedicine Online - April 6, 2019 Category: Reproduction Medicine Source Type: research

Triggering Method in Assisted Reproduction Alters the Cumulus Cell Transcriptome
ConclusionsThis is the first study to delineate key transcriptomic changes under dual triggering of final oocyte maturation, across different patient populations. Our findings underline the need for larger scale studies validating transcriptomic effects of methods for triggering final oocyte maturation. Furthermore, there is need in large scale clinical randomized controlled study to correlate our findings with clinical outcomes. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - April 6, 2019 Category: Reproduction Medicine Source Type: research

Artificial oocyte activation improves cycles with prospects of ICSI fertilization failure: a sibling oocyte control study
In this study, sibling oocytes from patients with previous ICSI failure or severe teratozoospermia were divided equally into two parts, half for artificial oocyte activation (AOA) with ionomycin after conventional ICSI and the other half for conventional ICSI only (non-AOA). We primarily compared the fertilization rates, cleavage rates, transferrable embryo rates and blastulation rates of the two groups and secondarily the pregnancy and live birth rates to verify the efficiency and safety of AOA.ResultThe outcomes of the AOA group were significantly better than those of the conventional ICSI group in terms of the fertiliza...
Source: Reproductive BioMedicine Online - April 6, 2019 Category: Reproduction Medicine Source Type: research

Births from embryos with highly elevated levels of mitochondrial dna
ConclusionsMeasurement of mtDNA copy number might not provide any advantage to embryo prioritization and could lead to de-selection of blastocysts that would result in healthy pregnancies and births. Furthermore, the quantitation of mitochondrial functional output in a model of cellular stress might suggest that mitochondria are not clear targets for biomarker identification as it relates to blastocyst viability. Any suggested link between mtDNA levels, mitochondria, or their output with blastocyst transfer outcome requires further validation. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - April 5, 2019 Category: Reproduction Medicine Source Type: research

GnRH agonist treatment of luteal phase deficiency in HCG-triggered IVF cycles: a matched case-control study
ConclusionsIn patients with LPD, the administration of GnRH agonist after embryo transfer increases serum progesterone concentration and improves the chance of pregnancy and birth. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - April 5, 2019 Category: Reproduction Medicine Source Type: research

Follicle stimulating hormone or clomiphene citrate in intrauterine insemination with ovarian stimulation for unexplained subfertility: a role for treatment selection markers?
ConclusionIn couples with unexplained subfertility undergoing IUI, no baseline treatment selection markers could be identified to determine whether ovaries should be stimulated with FSH or clomiphene citrate. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - April 3, 2019 Category: Reproduction Medicine Source Type: research

Intrauterine insemination: simultaneous with or 36 hours after hCG? A randomised clinical trial
ConclusionsThis multicenter RCT did not demonstrate that IUI performed at the moment of hCG-triggering increases pregnancy rates compared to IUI performed around the moment of ovulation.Trial registration numberPortal Toetsing Online Kenmerk NL39738.068.12. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - April 2, 2019 Category: Reproduction Medicine Source Type: research