Artificial oocyte activation improves cycles with prospects of ICSI fertilization failure: a sibling oocyte control study
In this study, sibling oocytes from patients with previous ICSI failure or severe teratozoospermia were divided equally into two parts, half for artificial oocyte activation (AOA) with ionomycin after conventional ICSI and the other half for conventional ICSI only (non-AOA). We primarily compared the fertilization rates, cleavage rates, transferrable embryo rates and blastulation rates of the two groups and secondarily the pregnancy and live birth rates to verify the efficiency and safety of AOA.ResultThe outcomes of the AOA group were significantly better than those of the conventional ICSI group in terms of the fertiliza...
Source: Reproductive BioMedicine Online - April 6, 2019 Category: Reproduction Medicine Source Type: research

Births from embryos with highly elevated levels of mitochondrial dna
ConclusionsMeasurement of mtDNA copy number might not provide any advantage to embryo prioritization and could lead to de-selection of blastocysts that would result in healthy pregnancies and births. Furthermore, the quantitation of mitochondrial functional output in a model of cellular stress might suggest that mitochondria are not clear targets for biomarker identification as it relates to blastocyst viability. Any suggested link between mtDNA levels, mitochondria, or their output with blastocyst transfer outcome requires further validation. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - April 5, 2019 Category: Reproduction Medicine Source Type: research

GnRH agonist treatment of luteal phase deficiency in HCG-triggered IVF cycles: a matched case-control study
ConclusionsIn patients with LPD, the administration of GnRH agonist after embryo transfer increases serum progesterone concentration and improves the chance of pregnancy and birth. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - April 5, 2019 Category: Reproduction Medicine Source Type: research

Follicle stimulating hormone or clomiphene citrate in intrauterine insemination with ovarian stimulation for unexplained subfertility: a role for treatment selection markers?
ConclusionIn couples with unexplained subfertility undergoing IUI, no baseline treatment selection markers could be identified to determine whether ovaries should be stimulated with FSH or clomiphene citrate. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - April 3, 2019 Category: Reproduction Medicine Source Type: research

Intrauterine insemination: simultaneous with or 36 hours after hCG? A randomised clinical trial
ConclusionsThis multicenter RCT did not demonstrate that IUI performed at the moment of hCG-triggering increases pregnancy rates compared to IUI performed around the moment of ovulation.Trial registration numberPortal Toetsing Online Kenmerk NL39738.068.12. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - April 2, 2019 Category: Reproduction Medicine Source Type: research

The Progestin-Primed Ovarian Stimulation (PPOS). Progestins instead of GnRH analogues to inhibit spontaneous ovulation during ovarian stimulation: is the beginning of a new era?
Publication date: Available online 29 March 2019Source: Reproductive BioMedicine OnlineAuthor(s): Antonio La Marca, Martina CapuzzoAbstractAdvancements in techniques for freezing oocytes and embryos in the field of assisted reproduction prompted new approaches to ovarian stimulation. A growing attention has been dedicated to progesterone and its derivatives to block the LH surge, since oocyte vitrification permits to remove the concerns about the possible harmful effect of progestins on endometrial receptivity. This review summarizes the state of art in the use of progestins to inhibit ovulation in ovarian stimulation prot...
Source: Reproductive BioMedicine Online - March 30, 2019 Category: Reproduction Medicine Source Type: research

Editorial Board
Publication date: April 2019Source: Reproductive BioMedicine Online, Volume 38, Issue 4Author(s): (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - March 30, 2019 Category: Reproduction Medicine Source Type: research

Contents
Publication date: April 2019Source: Reproductive BioMedicine Online, Volume 38, Issue 4Author(s): (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - March 30, 2019 Category: Reproduction Medicine Source Type: research

A comparison of two months pretreatment with GnRH agonists with or without an aromatase inhibitor in women with ultrasound-diagnosed ovarian endometriomas undergoing IVF
ConclusionsThe combination of depo-leuprolide acetate monthly for 60 days combined with daily letrozole has better clinical outcomes at IVF in women with endometriomas than depo-leuprolide acetate treatment alone. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - March 30, 2019 Category: Reproduction Medicine Source Type: research

Protective effect of oestrogen receptor α-PvuII transition against idiopathic male infertility: a case-control study and meta-analysis
ConclusionThe ERα-PvuII polymorphism could be considered as a possible protective factor against male infertility. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - March 30, 2019 Category: Reproduction Medicine Source Type: research

Childbirth close to natural menopause: does age at menopause matter?
ConclusionsMore than half of the women with menopause before the age of 45 gave birth within 10 years prior to natural menopause, whereas that was true for virtually no women with menopause at the age of 55 or older. Thus, the length of the sterile interval prior to natural menopause may vary by age at menopause. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - March 30, 2019 Category: Reproduction Medicine Source Type: research

Cytometric assessment of uterine receptivity via epithelial β3 integrin expression
ConclusionsThe distribution patterns and percentage expression of EB3 assessed by a flow cytometry grading system shows a significant relationship to success rates in ART cycles. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - March 30, 2019 Category: Reproduction Medicine Source Type: research

A meta-analysis of pregnancy-related outcomes and complications in women with polycystic ovary syndrome undergoing in vitro fertilization
This study provides an update and comprehensive evidence to support the observation that despite the fact that PCOS patients achieve better clinical pregnancy rate and live birth rate, physicians should continue to consider patients with PCOS to be high risk of adverse pregnancy-related outcomes. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - March 30, 2019 Category: Reproduction Medicine Source Type: research

Live birth rate and neonatal outcome following cleavage-stage embryo transfer versus blastocyst transfer using the freeze-all strategy
ConclusionsThere is no evidence to support the superiority of blastocyst transfer compared with cleavage-stage transfer in a freeze-all treatment scenario. There may be a higher risk of preterm singleton delivery following blastocyst transfer than following cleavage-stage transfer but further studies are needed to verify this. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - March 28, 2019 Category: Reproduction Medicine Source Type: research

Association between progesterone to number of mature oocytes index and live birth in GnRH antagonist protocols
Publication date: Available online 23 January 2019Source: Reproductive BioMedicine OnlineAuthor(s): Cynthia Simon, Lise Branet, Jessika Moreau, Nicolas Gatimel, Clementine Cohade, Florence Lesourd, Jean Parinaud, Roger LeandriAbstractResearch questionWhat is the association between blood progesterone level alone, progesterone to mature oocytes index (PMOI) and IVF outcomes?DesignClinical data from 960 couples undergoing their first fresh embryo transfer after an intracytoplasmic sperm injection attempt carried out between September 2012 and July 2017 were analysed. All patients underwent ovarian stimulation combining recom...
Source: Reproductive BioMedicine Online - March 28, 2019 Category: Reproduction Medicine Source Type: research

Comparison of perinatal outcomes following blastocyst and cleavage-stage embryo transfer: analysis of 10 years’ data from a single centre
Publication date: Available online 25 January 2019Source: Reproductive BioMedicine OnlineAuthor(s): Wenhao Shi, Wei Zhang, Na Li, Xia Xue, Chen Liu, Pengfei Qu, Juanzi Shi, Chen HuangAbstractResearch questionAre there greater risks of adverse perinatal outcomes, in particular of congenital malformations, after blastocyst transfer compared with cleavage-stage embryo transfer in IVF?DesignThis was a retrospective cohort analysis from a centre for assisted reproduction at a public hospital in China over the period 2006–2015. The analysis covered all women who conceived (15,254) and newborns (16,213) from IVF/intracytopl...
Source: Reproductive BioMedicine Online - March 28, 2019 Category: Reproduction Medicine Source Type: research

Human growth hormone for poor responders: a randomized placebo-controlled trial provides no evidence for improved live birth rate
Publication date: Available online 27 February 2019Source: Reproductive BioMedicine OnlineAuthor(s): Robert J. Norman, Helen Alvino, Louise M. Hull, Ben W. Mol, Roger J. Hart, Thu-Lan Kelly, Luk Rombauts, LIGHT investigatorsAbstractResearch questionDoes the addition of human growth hormone (HGH) to an IVF cycle improve the live birth rate in previously documented poor responders to FSH?DesignDouble-blind, placebo-controlled, randomized clinical trial comparing HGH to placebo in maximal stimulation in an IVF cycle. The study was stopped after 4 years. Women receiving ovarian stimulation in one IVF cycle, having failed to pr...
Source: Reproductive BioMedicine Online - March 28, 2019 Category: Reproduction Medicine Source Type: research

Biological differences between intrinsic and extrinsic adenomyosis with coexisting deep infiltrating endometriosis
Publication date: Available online 27 March 2019Source: Reproductive BioMedicine OnlineAuthor(s): Khaleque N Khan, Akira Fujishita, Akemi Koshiba, Haruo Kuroboshi, Taisuke Mori, Hiroshi Ogi, Kyoko Itoh, Masahiro Nakashima, Jo KitawakiAbstractResearch questionIs there any biological difference between intrinsic and extrinsic adenomyosis with coexisting deep infiltrating endometriosis (DIE)?DesignIn this prospective controlled study, biopsy specimens were collected after surgery from 23 women with intrinsic adenomyosis and 10 women with extrinsic adenomyosis with coexisting DIE lesions. A histological search was done by immu...
Source: Reproductive BioMedicine Online - March 28, 2019 Category: Reproduction Medicine Source Type: research

Beneficial effects of dietary nitrate supplementation on testicular injury in streptozotocin-induced diabetic male rats
Publication date: Available online 8 January 2019Source: Reproductive BioMedicine OnlineAuthor(s): Rana Keyhanmanesh, Gholamreza Hamidian, Mohammad Reza Alipour, Hajar OghbaeiAbstractResearch questionDo low doses of dietary nitrate help to attenuate the progression of diabetic reproductive disorders in streptozotocin-induced diabetic male rats?DesignFifty male Wistar rats were divided into five groups: controls receiving distilled water; controls receiving 100 mg/l nitrate in distilled water; diabetic rats receiving distilled water; diabetic rats receiving insulin 2–4 U/day of neutral protamine hagedorn insulin; and ...
Source: Reproductive BioMedicine Online - March 27, 2019 Category: Reproduction Medicine Source Type: research

Chief editor’s 2018 annual report
Publication date: Available online 19 March 2019Source: Reproductive BioMedicine OnlineAuthor(s): Bart CJM Fauser, Kamal K Ahuja (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - March 21, 2019 Category: Reproduction Medicine Source Type: research

Management of Thin Endometrium in Assisted Reproduction (AR)
The objective of this guideline is to provide evidence-based recommendations using the GRADE (Grading of Recommendations, Assessment, Development and Evaluations) framework on the assessment, impact and management of thin endometrium in assisted reproduction. The effect of endometrial thickness on pregnancy and live birth outcomes in controlled ovarian stimulation and in-vitro fertilization (fresh and frozen cycles) is addressed. In addition, recommendations on the use of adjuvants to improve endometrial thickness and pregnancy outcomes are provided. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - March 21, 2019 Category: Reproduction Medicine Source Type: research

Chief editor's 2018 annual report
Publication date: Available online 19 March 2019Source: Reproductive BioMedicine OnlineAuthor(s): Bart CJM Fauser, Kamal K Ahuja (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - March 21, 2019 Category: Reproduction Medicine Source Type: research

Influence of Resveratrol Supplementation on Pregnancy Outcomes in in vitro Fertilization–Embryo Transfer Cycles
ConclusionsResveratrol supplementation during embryo transfer cycles appears to be detrimental for pregnancy outcomes. To be clinically useful, an analysis of the supplementation protocol and randomized controlled study are needed. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - March 21, 2019 Category: Reproduction Medicine Source Type: research

Is polycystic ovary syndrome associated with risk of female sexual dysfunction? A systematic review and meta-analysis
Publication date: Available online 8 January 2019Source: Reproductive BioMedicine OnlineAuthor(s): Shankun Zhao, Jiamin Wang, Qiang Xie, Lianmin Luo, Zhiguo Zhu, Yangzhou Liu, Jintai Luo, Zhigang ZhaoAbstractThe aim of this study was to evaluate whether polycystic ovary syndrome (PCOS) is a risk factor for female sexual dysfunction (FSD) by conducting a systematic review and meta-analysis. The databases PubMed, EMBASE and the Cochrane Library were searched for relevant studies. The association between PCOS and risk of FSD was assessed by relative risk or standard mean differences with 95% confidence interval. The protocol ...
Source: Reproductive BioMedicine Online - March 19, 2019 Category: Reproduction Medicine Source Type: research

NovelCFAP43 andCFAP44 mutations cause male infertility with multiple morphological abnormalities of the sperm flagella (MMAF)
ConclusionsThis study demonstrated that CFAP43 and CFAP44 mutations are important causes of MMAF in the Chinese population. These novel mutations broaden the spectrum of CFAP43 and CFAP44 mutations. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - March 18, 2019 Category: Reproduction Medicine Source Type: research

A comparison among 36 assisted reproduction laboratories monitoring the environmental conditions and instrument parameters using the same quality control application
ConclusionsThere are major differences in instrument monitoring practices among laboratories. While incubator monitoring is the largest domain, there are many other sensitive instruments that are diligently monitored by most laboratories. There is a need for international standardization and guidelines. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - March 18, 2019 Category: Reproduction Medicine Source Type: research

Influence of oral vitamin and mineral supplementation on male infertility: a meta-analysis and systematic review
Publication date: Available online 16 March 2019Source: Reproductive BioMedicine OnlineAuthor(s): Kai J. Buhling, Annika Schumacher, Christine zu Eulenburg, Elena LaakmannAbstractTo study the evidence of the influence of micronutrient supplementation on male fertility based on published research and to make recommendations for micronutrient supplementation for men suffering from infertility.18 randomised, double-blind, placebo-controlled trials were included in the meta-analysis or in the systematic review.A significant improvement in semen parameters was evident for selenium (200 g orally, daily) and L-carnitine (LC; 2 g ...
Source: Reproductive BioMedicine Online - March 18, 2019 Category: Reproduction Medicine Source Type: research

Evaluation of the cost-effectiveness of ovulation suppression with progestins compared with GnRH analogs in assisted reproduction cycles
ConclusionsOvulation inhibition in IVF using progestins requires a freeze-only approach of embryos, and thus progestin use was not cost-effective compared with fresh embryo transfer cycles. Progestins, however, may be cost-effective compared with GnRH antagonist in planned freeze only cycles such as in preimplantation genetic testing or fertility preservation. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - March 16, 2019 Category: Reproduction Medicine Source Type: research

Consultation and treatment behaviour of infertile couples in China: a population-based study
ConclusionThe prevalence of infertility in China is high, but the uptake of treatment is relatively low. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - March 15, 2019 Category: Reproduction Medicine Source Type: research

A multicentre evaluation of the Elecsys® anti-Müllerian hormone immunoassay for prediction of antral follicle count
Conclusion: The Elecsys AMH immunoassay provides a robust and fully automated method to measure serum AMH levels. Women with AMH values below the cut-off of 1.77 ng/ml are unlikely to have AFC>15. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - March 15, 2019 Category: Reproduction Medicine Source Type: research

Follicular fluid progesterone concentration is associated with fertilization outcome after IVF: a systematic review and meta-analysis
Publication date: Available online 17 January 2019Source: Reproductive BioMedicine OnlineAuthor(s): Bernadett Nagy, Laszlo Poto, Nelli Farkas, Miklos Koppan, Akos Varnagy, Kalman Kovacs, Szilard Papp, Noemi Bohonyi, Jozsef BodisAbstractFollicular fluid is a key biochemical environment for oocyte development. The potential effect of follicular progesterone level on successful fertilization is a subject of debate, and so the aim of this study was to provide a summary of the currently available evidence on the association between follicular fluid progesterone level and fertilization outcome. To do so, a systematic review and ...
Source: Reproductive BioMedicine Online - March 13, 2019 Category: Reproduction Medicine Source Type: research

Predictive value of serum kisspeptin levels on 14 and 21 days after frozen-thawed embryo transfer
Publication date: Available online 12 March 2019Source: Reproductive BioMedicine OnlineAuthor(s): Kai-Lun Hu, Yongli Zhang, Zi Yang, Hongcui Zhao, Huiyu Xu, Yang Yu, Rong LiAbstractResearch QuestionWhether the serum kisspeptin levels on 14 and 21 days after frozen-thawed embryo transfer (ET) could predict the early pregnant outcome of patients.DesignProspective study. 133 patients undergoing in vitro fertilization (IVF) and frozen-thawed ET enrolled. Patients are divided into non-pregnant (NP) group and pregnant group (including biochemical pregnancy (BP) group, single pregnancy (SP) group, Miscarriage group, and Twin grou...
Source: Reproductive BioMedicine Online - March 13, 2019 Category: Reproduction Medicine Source Type: research

Differential impacts of gonadotropins, in vitro fertilization and mouse embryo culture on blastocyst development
This study examined developmental characteristics of mouse blastocysts derived from ART compared to in vivo-conceived embryos. To determine the effect of ovarian stimulation versus in vitro fertilization (IVF) versus in vitro embryo culture on phenotype, six distinct groups of blastocysts were generated. Female mice were naturally cycling or treated with high or mild doses of gonadotropin; followed by natural mating or IVF in clinical conditions. Embryo morphokinetics were assessed by continuous time-lapse monitoring. Cell lineage allocation to inner cell mass (Oct4+) or trophectoderm (Cdx2+) was determined by immunohistoc...
Source: Reproductive BioMedicine Online - March 13, 2019 Category: Reproduction Medicine Source Type: research

Maternal β-HCG concentrations in early IVF pregnancy: association with the embryo development stage of blastocysts
Publication date: Available online 8 January 2019Source: Reproductive BioMedicine OnlineAuthor(s): Wenyue Zhu, Queenie Yeung, David Chan, Ling Chi, Jin Huang, Qiong Wang, Jacqueline Chung, Tin-Chiu LiAbstractResearch questionDoes the blastocyst development stage on embryo transfer day affect serum β-human chorionic gonadotrophin (β-HCG) concentration 9 and 16 days after embryo transfer in women whose pregnancy progresses beyond 13 weeks?DesignRetrospective study involving 204 cases where pregnancy progressed beyond 13 weeks of single blastocyst transfer in fresh (n= 66) or frozen embryo transfer (FET) (n=&nb...
Source: Reproductive BioMedicine Online - March 13, 2019 Category: Reproduction Medicine Source Type: research

‘Is blood thicker than water?’ Donor conceived offspring’s subjective experiences of the donor: a systematic narrative review
Publication date: Available online 8 January 2019Source: Reproductive BioMedicine OnlineAuthor(s): Elena Canzi, Monica Accordini, Federica FacchinAbstractThe aim of this study was to provide a systematic narrative review of the published research articles on donor conceived offspring’s subjective experiences of their donor. A systematic search of electronic databases (PsycINFO and PubMed) provided 29 published quantitative and qualitative studies matching the inclusion criteria. The analysis of the studies outcomes led to the identification of four main themes: (i) views/representations of the donor; (ii) desire to c...
Source: Reproductive BioMedicine Online - March 13, 2019 Category: Reproduction Medicine Source Type: research

Evaluation of preimplantation genetic testing based on next-generation sequencing for balanced reciprocal translocation carriers
ConclusionsCNV-Seq-based NGS is an efficient and reliable PGT method for balanced reciprocal translocation. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - March 13, 2019 Category: Reproduction Medicine Source Type: research

The effect of a GnRH antagonist (Ganirelix) on follicle maturation in normal women
ConclusionsThis study demonstrates for the first time that in normal women dominant follicle selection failed during treatment with Ganirelix. As there was a similar gonadotrophin profile in the two cycles, it is suggested that Ganirelix interferes with the process of follicle selection by acting in the ovary. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - March 13, 2019 Category: Reproduction Medicine Source Type: research

Controversies in ART: Considerations and Risks for Uninterrupted Embryo Culture
Publication date: Available online 11 March 2019Source: Reproductive BioMedicine OnlineAuthor(s): Jason E. SwainAbstractWith new time-lapse incubators, IVF laboratories have increased use of single step media, often used in an uninterrupted approach. This simplifies the culture process for embryologists, may help reduce costs, and offers the potential to reduce cell handling and associated harmful environmental stressors and improve embryo quality and outcomes. However, one could argue that optimized quality control and culture conditions are required to successfully implement uninterrupted culture. Without impeccable labo...
Source: Reproductive BioMedicine Online - March 13, 2019 Category: Reproduction Medicine Source Type: research

Individual culture and atmospheric oxygen during culture affect mouse preimplantation embryo metabolism and postimplantation development
ConclusionsPreimplantation metabolism of glucose and amino acids is altered by both oxygen and individual culture, and fetal weight is reduced by individual culture under atmospheric oxygen but not 5% oxygen. This study raises concerns regarding the increasing prevalence of single embryo culture in human IVF and adds to the existing evidence regarding the detrimental effects of atmospheric oxygen during embryo culture. Furthermore, these data demonstrate the cumulative nature of stress during embryo culture and highlight the importance of optimising each element of the culture system. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - March 13, 2019 Category: Reproduction Medicine Source Type: research

Effect of pretreatment with Levonorgestrel-Releasing Intrauterine System on IVFoutcomes in Women with Adenomyosis
ConclusionsIt offers some support for evaluating the effect of pretreatment with Levonorgestrel-Releasing Intrauterine System in women with adenomyosis in future randomized controlled trials(RCTs). (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - March 13, 2019 Category: Reproduction Medicine Source Type: research

Early vs. delayed follow-up after misoprostol treatment for early pregnancy loss
Publication date: Available online 12 March 2019Source: Reproductive BioMedicine OnlineAuthor(s): Yossi Mizrachi, Liliya Tamayev, Ofer Shemer, Ilia Kleiner, Jacob Bar, Ron SagivAbstractResearch QuestionDoes extending the follow-up after misoprostol treatment for early pregnancy loss increase the success rate.DesignWe prospectively followed patients with early pregnancy loss (
Source: Reproductive BioMedicine Online - March 13, 2019 Category: Reproduction Medicine Source Type: research

The relative importance of genetic parenthood
ConclusionsWhile awaiting replication of this study in larger populations, these findings challenge the presumed dominant importance of genetic parenthood. This raises questions about whether donor gametes could be presented as a worthy alternative earlier in treatment trajectories and whether investments in novel treatments enabling genetic parenthood, like in vitro gametogenesis, are proportional to their future clinical impact. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - March 13, 2019 Category: Reproduction Medicine Source Type: research

Inheritance of imbalances in the recurrent chromosomal translocation t(11;22) in female and male carriers: Clarification by using combined PGD and sperm FISH analysis
Conclusion(s)Genetic counselling must take into consideration the risk of a birth related to the adjacent-1 segregation mode, irrespective the translocation carrier sex. These results will allow a deeper understanding of the PGD results of t(11;22) carriers, which often include a high number of aneuploid embryos. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - March 13, 2019 Category: Reproduction Medicine Source Type: research

Pre-implantation genetic testing for aneuploidy does not decrease three-cycle cumulative discontinuation rate when compared to conventional in-vitro fertilization in women with advanced maternal age
Publication date: Available online 12 March 2019Source: Reproductive BioMedicine OnlineAuthor(s): Sezcan Mumusoglu, Irem Yarali Ozbek, Zuhal Yapici Coskun, Mehtap Polat, Lale Karakoc Sokmensuer, Gurkan Bozdag, Hakan YaraliAbstractResearch QuestionDoes pre-implantation genetic testing for aneuploidy (PGT-A) influence discontinuation rate in women with advanced maternal age (AMA) undergoing in-vitro fertilization (IVF)?DesignRetrospective longitudinal cohort study carried-out at a single IVF clinic in Turkey. In total, 401 consecutive AMA cases were included. Discontinuation rates of pre-intervention (Conventional-IVF, June ...
Source: Reproductive BioMedicine Online - March 13, 2019 Category: Reproduction Medicine Source Type: research

Effect of recombinant human vascular endothelial growth factor (VEGF-165) on testis tissue xenotransplants from prepubertal boys: A three-case study
Publication date: Available online 12 March 2019Source: Reproductive BioMedicine OnlineAuthor(s): Elissavet Ntemou, Prashant Kadam, Sven Van Laere, Dorien Van Saen, Elena Vicini, Ellen GoossensABSTRACTResearch questionDoes recombinant human vascular endothelial growth factor (VEGF-165) improve the efficiency of human immature testis tissue (ITT) xenotransplantation?DesignITT fragments from 3 prepubertal boys were cultured for 5 days with VEGF-165 or without (control) prior to xenotransplantation into the testes of immunodeficient mice. Xenotransplants were recovered at 4 and 9 months post-transplantation and vascularisatio...
Source: Reproductive BioMedicine Online - March 13, 2019 Category: Reproduction Medicine Source Type: research

Endometrial injury increases expression of hypoxia-inducible factor and angiogenesis in the endometrium of women with recurrent implantation failure
ConclusionsHIF-1α expression, MVD and endometrial apoptosis were reduced in the peri-implantation endometrium of women with RIF. This suggests that altered endometrial HIF-1α expression and angiogenesis may contribute to implantation failure. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - March 6, 2019 Category: Reproduction Medicine Source Type: research

FSH or CC in IUI with ovarian stimulation for unexplained subfertility: a role for treatment selection markers?
ConclusionWe could not identify treatment selection markers at baseline in couples with unexplained subfertility to decide on ovarian stimulation with FSH instead of CC in IUI. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - March 4, 2019 Category: Reproduction Medicine Source Type: research

Mutational analysis of FAM175A gene in patients with premature ovarian insufficiency
ConclusionOur results suggest that p.L243V variant in FAM175A gene may be not causative for POI. The contribution of FAM175A to POI needs further exploration. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - March 1, 2019 Category: Reproduction Medicine Source Type: research

Absence of predictable long-term molecular effect of ulipristal acetate (UPA) on the endometrium
ConclusionThe effect of 3-month UPA treatment is mostly progesterone receptor antagonist-like. After treatment is discontinued, there are no signs of any long-term effects of this molecule on endometrial proliferation and maturation. Therefore, UPA may be administered to women willing to conceive in the short term without consequences for further implantation. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - February 28, 2019 Category: Reproduction Medicine Source Type: research

Pregnancy outcomes after recurrent pregnancy loss: a longitudinal cohort study on stress and depression
ConclusionsSelf-reported emotional distress did not affect future chance of live birth. A live born child decreased emotional distress. (Source: Reproductive BioMedicine Online)
Source: Reproductive BioMedicine Online - February 27, 2019 Category: Reproduction Medicine Source Type: research