Mechanism of action of pseudopteroxazole and pseudopterosin G: Diterpenes from marine origin
In this study, we investigated the modes of action of Ptx and pseudopterosin  G (PsG) inBacillus subtilis employing an unbiased approach that combines gel-based proteomics with a mathematical similarity analysis of response profiles. Proteomic responses to sublethal concentrations of Ptx and PsG were compared to a library of antibiotic stress response profiles revealing that both induce a stress response characteristic for agents targeting the bacterial cell envelope by interfering with membrane-bound steps of cell wall biosynthesis. Microscopy-based assays confirmed that both compounds compromise the integrity of the bac...
Source: Proteomics - January 10, 2024 Category: Biochemistry Authors: Niklas B. M. Janzing, Christoph H. R. Senges, Pascal Dietze, Bradley Haltli, Douglas H. Marchbank, Russell G. Kerr, Julia E. Bandow Tags: RESEARCH ARTICLE Source Type: research

Changes in the astronaut serum proteome during prolonged spaceflight
This study will allow us to accelerate research to improve our understanding of the molecular mechanisms of biological adaptations associated with prolonged spaceflight. (Source: Proteomics)
Source: Proteomics - January 8, 2024 Category: Biochemistry Authors: Yayoi Kimura, Yusuke Nakai, Yoko Ino, Tomoko Akiyama, Kayano Moriyama, Tatsuya Aiba, Takashi Ohira, Kenji Egashira, Yu Yamamoto, Yuriko Takeda, Yutaka Inaba, Akihide Ryo, Tomoyuki Saito, Ken Kumagai, Hisashi Hirano Tags: RESEARCH ARTICLE Source Type: research

Proteome profile of the cerebellum from α7 nicotinic acetylcholine receptor deficient mice
In conclusion, our dataset provides new insights in the role of CHRNA7 in the cerebellum, which may serve as a basis for future in depth-investigations. (Source: Proteomics)
Source: Proteomics - January 8, 2024 Category: Biochemistry Authors: Karolina Magdalena Caban, Pia Se ßenhausen, Jan Bernard Stöckl, Bastian Popper, Artur Mayerhofer, Thomas Fröhlich Tags: DATASET BRIEF Source Type: research

Proteomic profiling of ovarian clear cell carcinomas identifies prognostic biomarkers for chemotherapy
AbstractClear cell ovarian carcinoma (CCOC) is a relatively rare subtype of ovarian cancer (OC) with high degree of resistance to standard chemotherapy. Little is known about the underlying molecular mechanisms, and it remains a challenge to predict its prognosis after chemotherapy. Here, we first analyzed the proteome of 35 formalin-fixed paraffin-embedded (FFPE) CCOC tissue specimens from a cohort of 32 patients with CCOC (H1 cohort) and characterized 8697 proteins using data-independent acquisition mass spectrometry (DIA-MS). We then performed proteomic analysis of 28 fresh frozen (FF) CCOC tissue specimens from an inde...
Source: Proteomics - January 4, 2024 Category: Biochemistry Authors: Liang Yue, Tingting Gong, Wenhao Jiang, Liujia Qian, Wangang Gong, Yaoting Sun, Xue Cai, Heli Xu, Fanghua Liu, He Wang, Sainan Li, Yi Zhu, Zhiguo Zheng, Qijun Wu, Tiannan Guo Tags: RESEARCH ARTICLE Source Type: research

Analysis of N ‐ and O‐linked site‐specific glycosylation by ion mobility mass spectrometry: State of the art and future directions
AbstractGlycosylation, the major post-translational modification of proteins, significantly increases the diversity of proteoforms. Glycans are involved in a variety of pivotal structural and functional roles of proteins, and changes in glycosylation are profoundly connected to the progression of numerous diseases. Mass spectrometry (MS) has emerged as the gold standard for glycan and glycopeptide analysis because of its high sensitivity and the wealth of fragmentation information that can be obtained. Various separation techniques have been employed to resolve glycan and glycopeptide isomers at the front end of the MS. Ho...
Source: Proteomics - January 4, 2024 Category: Biochemistry Authors: Michael Girgis, Gregory Petruncio, Paul Russo, Steven Peyton, Mikell Paige, Diana Campos, Miloslav Sanda Tags: REVIEW Source Type: research

A crustacean neuropeptide spectral library for data ‐independent acquisition (DIA) mass spectrometry applications
AbstractNeuropeptides have tremendous potential for application in modern medicine, including utility as biomarkers and therapeutics. To overcome the inherent challenges associated with neuropeptide identification and characterization, data-independent acquisition (DIA) is a fitting mass spectrometry (MS) method of choice to achieve sensitive and accurate analysis. It is advantageous for preliminary neuropeptidomic studies to occur in less complex organisms, with crustacean models serving as a popular choice due to their relatively simple nervous system. With spectral libraries serving as a means to interpret DIA-MS output...
Source: Proteomics - January 4, 2024 Category: Biochemistry Authors: Lauren Fields, Min Ma, Kellen DeLaney, Ashley Phetsanthad, Lingjun Li Tags: DATASET BRIEF Source Type: research

The multifaceted roles of small extracellular vesicles in metabolic reprogramming in the tumor microenvironments
AbstractThe link between metabolism and tumor progression has been extensively researched for a long time. With the increasing number of studies uncovering the multiple  functions of metabolic reprogramming in tumor microenvironments, the regulatory network seems to become even more intricate at the same time. Small extracellular vesicles (sEV), as crucial mediators facilitating intercellular communications, exhibit significant involvement in regulating metabolic reprogramming within the complicated network of tumor microenvironments. sEV derived from tumor cells and those released by other cell populations such as tumor-...
Source: Proteomics - January 4, 2024 Category: Biochemistry Authors: Zhixian Chen, Judy Wai Ping Yam, Xiaowen Mao Tags: REVIEW Source Type: research

Defining the relationship between cellular and extracellular vesicle (EV) content in breast cancer via an integrative multi ‐omic analysis
AbstractMuch recent research has been dedicated to exploring the utility of extracellular vesicles (EVs) as circulating disease biomarkers. Underpinning this work is the assumption that the molecular cargo of EVs directly reflects the originating cell. Few attempts have been made, however, to empirically validate this on the -omic level. To this end, we have performed an integrative multi-omic analysis of a panel of breast cancer cell lines and corresponding EVs. Whole transcriptome analysis validated that the cellular transcriptome remained stable when cultured cells are transitioned to low serum or serum-free medium for ...
Source: Proteomics - January 3, 2024 Category: Biochemistry Authors: Rebecca E. Lane, Darren Korbie, Kum Kum Khanna, Ahmed Mohamed, Michelle M. Hill, Matt Trau Tags: RESEARCH ARTICLE Source Type: research