Expression and characterization of a novel trehalase from Microvirga sp. strain MC18
Publication date: Available online 13 February 2021Source: Protein Expression and PurificationAuthor(s): Chaonan Dong, Qiwen Fan, Xu Li, Yan Huang, Jian Han, Xiaodong Fang, Minghui Huan, Xianfeng Ye, Zhoukun Li, Zhongli Cui (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - February 15, 2021 Category: Biochemistry Source Type: research

Functional expression and purification of recombinant full-length human ATG7 protein with HIV-1 Tat peptide in Escherichia coli
Publication date: Available online 13 February 2021Source: Protein Expression and PurificationAuthor(s): Guofu Dong, Xi Zhao, Junwang Guo, Lei Ma, Hongmei Zhou, Qi Liu, Xuelong Zhao, Changzhen Wang, Ke Wu (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - February 15, 2021 Category: Biochemistry Source Type: research

Removing a single-arm species by Fibro PrismA in purifying an asymmetric IgG-like bispecific antibody
Publication date: Available online 13 February 2021Source: Protein Expression and PurificationAuthor(s): Ting Zhang, Yan Wan, Ying Wang, Yifeng Li (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - February 15, 2021 Category: Biochemistry Source Type: research

Identification of chicken-derived scFv against N-glycolylneuraminic acid retrieved from an immune library by phage display
Publication date: Available online 11 February 2021Source: Protein Expression and PurificationAuthor(s): Han Wang, Zong-Cheng Wu, Pan Hu, Hong-Lin Ren, Yan-Song Li, Yu Zheng, Cong Wang, Zeng-Shan Liu, Shi-Ying Lu (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - February 13, 2021 Category: Biochemistry Source Type: research

A Custom ÄKTA Avant Configuration Enabling Automated Parallel Protein Purification over a Range of Process Scales
Publication date: Available online 11 February 2021Source: Protein Expression and PurificationAuthor(s): Cristopher Hollander, Shana C. Walrond, Christopher Connelly, Lisa Megson, Elia Bove, Thomas McDonagh (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - February 13, 2021 Category: Biochemistry Source Type: research

Expression and characterization of a thermotolerant and pH-stable hyaluronate lyase from Thermasporomyces composti DSM22891
Publication date: Available online 7 February 2021Source: Protein Expression and PurificationAuthor(s): Xiaoyi Wang, Shilong Zhang, Hao Wu, Yujiao Li, Wengong Yu, Feng Han (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - February 9, 2021 Category: Biochemistry Source Type: research

Purification, secondary structure and antioxidant activity of metallothionein zinc-binding proteins from Arca subcrenata
Publication date: Available online 6 February 2021Source: Protein Expression and PurificationAuthor(s): Chunying Meng, Kuiwu wang, Xiaojun zhang, Xinyue Zhu (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - February 7, 2021 Category: Biochemistry Source Type: research

Overexpression and purification of a toxic peptide LaIT2 from Japanese scorpion, Liocheles australasiae
Publication date: Available online 3 February 2021Source: Protein Expression and PurificationAuthor(s): Maiki Tamura, Chiharu Tatsushiro, Eugene Hayato Morita, Shinya Ohki (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - February 4, 2021 Category: Biochemistry Source Type: research

An improved production and purification protocol for recombinant soluble human fibroblast activation protein alpha
Publication date: Available online 30 January 2021Source: Protein Expression and PurificationAuthor(s): Cecy R. Xi, Arianna Di Fazio, Naveed Ahmed Nadvi, Michelle Sui Wen Xiang, Hui Emma Zhang, Chandrika Deshpande, Yiqian Chen, Mehdi Sharifi Tabar, Xin Maggie Wang, Charles G. Bailey, Geoffrey W. McCaughan, W Bret Church, Mark D. Gorrell (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - January 31, 2021 Category: Biochemistry Source Type: research

High Level Stable Expression of Recombinant HIV gp120 in Glutamine Synthetase Gene Deficient HEK293T Cells
Publication date: Available online 30 January 2021Source: Protein Expression and PurificationAuthor(s): Zhongcheng Zou, Ruipeng Wang, Eden P. Go, Heather Desaire, Peter D. Sun (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - January 31, 2021 Category: Biochemistry Source Type: research

Expression, purification and characterization of the second DUSP domain of deubiquitinase USP20/VDU2
Publication date: Available online 30 January 2021Source: Protein Expression and PurificationAuthor(s): Shuwei Hu, Xiaoyu Chen, Mengying Zhu, Huifang Hu, Chen Zhou, Li Shi, Naixia Zhang (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - January 31, 2021 Category: Biochemistry Source Type: research

Characterisation and comparative analysis of hydrophobin isolated from Pleurotus floridanus (PfH)
Publication date: Available online 29 January 2021Source: Protein Expression and PurificationAuthor(s): C.M. Rafeeq, A.B. Vaishnav, P.P. Manzur Ali (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - January 30, 2021 Category: Biochemistry Source Type: research

Sampling the cultivation parameter space for the bacterial production of TLR1 intracellular domain reveals the multiple optima
Publication date: Available online 29 January 2021Source: Protein Expression and PurificationAuthor(s): Marina V. Goncharuk, Vladislav A. Lushpa, Sergey A. Goncharuk, Alexander S. Arseniev, Konstantin S. Mineev (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - January 30, 2021 Category: Biochemistry Source Type: research

Medium Optimization for high yield production of human serum albumin in Pichia pastoris and its efficient purification
Publication date: Available online 26 January 2021Source: Protein Expression and PurificationAuthor(s): Wen Zhu, Renren Xu, Guihua Gong, Lei Xu, Youjia Hu, Liping Xie (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - January 27, 2021 Category: Biochemistry Source Type: research

PiP2 favors an α-helical structure of non-recombinant Hsp12 of Saccharomyces cerevisiae
Publication date: Available online 22 January 2021Source: Protein Expression and PurificationAuthor(s): Antoine Léger, Mehdi Azouz, Sophie Lecomte, François Dole, Agnès Hocquellet, Stéphane Chaignepain, Charlotte Cabanne (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - January 23, 2021 Category: Biochemistry Source Type: research