Protein Expression and Purification 
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This is an RSS file. You can use it to subscribe to this data in your favourite RSS reader or to display this data on your own website or blog.Expression, purification, and structural analysis of the full-length human integral membrane protein γ-sarcoglycan
Publication date: Available online 1 November 2019Source: Protein Expression and PurificationAuthor(s): Michael Jamaladdine, Michael S. Harris, Leshani Liyanage, Gabriel A. CookAbstractMutation of the gene encoding γ-sarcoglycan (SGCG), an integral membrane protein responsible for maintaining the integrity of the muscle cell sarcolemma, results in Limb-Girdle Muscular Dystrophy (LGMD), a congenital disease with no current treatment options. This member of the sarcoglycan glycoprotein family is a vital component of the Dystrophin Complex, which together facilitate normal muscle function. However, very little is known about...
Source: Protein Expression and Purification - November 3, 2019 Category: Biochemistry Source Type: research
Expression and purification of recombinant G protein-coupled receptors: A review
Publication date: Available online 31 October 2019Source: Protein Expression and PurificationAuthor(s): Daniel N. Wiseman, Abigail Otchere, Jaimin H. Patel, Romez Uddin, Naomi L. Pollock, Sarah J. Routledge, Alice J. Rothnie, Cathy Slack, David R. Poyner, Roslyn M. Bill, Alan D. GoddardAbstractGiven their extensive role in cell signalling, GPCRs are significant drug targets; despite this, many of these receptors have limited or no available prophylaxis. Novel drug design and discovery significantly rely on structure determination, of which GPCRs are typically elusive. Progress has been made thus far to produce sufficient q...
Source: Protein Expression and Purification - November 2, 2019 Category: Biochemistry Source Type: research
Molecular characterization of a highly efficient and thermostable phosphoribosyl anthranilate isomerase from Geobacillus thermopakistaniensis
Publication date: Available online 29 October 2019Source: Protein Expression and PurificationAuthor(s): Muhammad Arif, Qamar Bashir, Masood Ahmad Siddiqui, Naeem RashidAbstractPhosphoribosyl anthranilate isomerase is involved in the isomerization of phosphoribosyl anthranilate to 1-(o-carboxyphenylamino)-1-deoxyribulose 5-phosphate. In the present study, trpFGt, a gene encoding phosphoribosyl anthranilate isomerase from Geobacillus thermopakistaniensis, was cloned and expressed in Escherichia coli. The gene product, TrpFGt, was produced in E. coli in soluble and active form. Molecular characterization revealed that recombi...
Source: Protein Expression and Purification - October 31, 2019 Category: Biochemistry Source Type: research
Cloning, characterization and expression analysis of glutathione S-transferase from the Antarctic yeast Rhodotorula mucilaginosa AN5
Publication date: Available online 25 October 2019Source: Protein Expression and PurificationAuthor(s): Cuijuan Shi, Xiaofei Wang, Zijie Xiao, Ruiqi Wang, Yongping Qiao, Guangfeng KanAbstractThe gene for glutathione S-transferase (GST) in Antarctic sea-ice yeast Rhodotorula mucilaginosa AN5 was cloned and expressed in Escherichia coli and named RmGST. Sequence analysis showed that the RmGST gene contained a 843 bp open reading frame, which encoded 280 amino acid residues with a calculated molecular mass of 30.4 kDa and isoelectric point of 5.40. RmGST has the typical C- and N-terminal double domains of glutathione S-tran...
Source: Protein Expression and Purification - October 26, 2019 Category: Biochemistry Source Type: research
Strategies for successful isolation of a eukaryotic transporter
Publication date: Available online 23 October 2019Source: Protein Expression and PurificationAuthor(s): Savvas Saouros, Cristina Cecchetti, Alex Jones, Alexander D. Cameron, Bernadette ByrneAbstractThe isolation of integral membrane proteins for structural analysis remains challenging and this is particularly the case for eukaryotic membrane proteins. Here we describe our efforts to isolate OsBOR3, a boron transporter from Oryza sativa. OsBOR3 was expressed as both full length and a C-terminally truncated form lacking residues 643–672 (OsBOR3Δ1-642). While both express well as C-terminal GFP fusion proteins in Saccharom...
Source: Protein Expression and Purification - October 25, 2019 Category: Biochemistry Source Type: research
