STEP® vectors for rapid generation of stable transfected CHO cell pools and clones with high expression levels and product quality homogeneity of difficult-to-express proteins
Publication date: Available online 25 May 2021Source: Protein Expression and PurificationAuthor(s): Abhinav Luthra, Remco A. Spanjaard, Sarwat Cheema, Nathalie Veith, Lars Kober, Yiding Wang, Tao Jing, Yi Zhao, Femke Hoeksema, Chris Yallop, Menzo Havenga, Wilfried A.M. Bakker (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - May 26, 2021 Category: Biochemistry Source Type: research

Milligram scale expression, refolding, and purification of Bombyx mori cocoonase using a recombinant E. coli system
Publication date: Available online 24 May 2021Source: Protein Expression and PurificationAuthor(s): Chanrith Phoeurk, Ameeq Ul Mushtaq, Per Rogne, Magnus Wolf-Watz (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - May 25, 2021 Category: Biochemistry Source Type: research

Optimized expression and purification of a soluble BMP2 variant based on in-silico design
Publication date: Available online 24 May 2021Source: Protein Expression and PurificationAuthor(s): Tobias Heinks, Anette Hettwer, Christian Hiepen, Christoph Weise, Marcel Gorka, Petra Knaus, Thomas D. Mueller, Angelika Loidl-Stahlhofen (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - May 25, 2021 Category: Biochemistry Source Type: research

Sodium caprylate wash during Protein A chromatography as an effective means for removing protease(s) responsible for target antibody fragmentation
Publication date: Available online 20 May 2021Source: Protein Expression and PurificationAuthor(s): Lixia Hu, Jiaqin Tang, Xudong Zhang, Yifeng Li (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - May 21, 2021 Category: Biochemistry Source Type: research

Biochemical characterization and redesign of the coenzyme specificity of a novel monofunctional NAD+-dependent homoserine dehydrogenase from the human pathogen Neisseria gonorrhoeae
Publication date: Available online 20 May 2021Source: Protein Expression and PurificationAuthor(s): Wanggang Tang, Xue Dong, Jiang Meng, Yanan Feng, Manman Xie, Haonan Xu, Ping Song (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - May 21, 2021 Category: Biochemistry Source Type: research

Characterization of recombinant E. coli expressing a novel fucosidase from Bacillus cereus 2–8 belonging to GH95 family
Publication date: October 2021Source: Protein Expression and Purification, Volume 186Author(s): Qian Li, Chaofeng Jiang, Haidong Tan, Xiaoming Zhao, Kuikui Li, Heng Yin (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - May 21, 2021 Category: Biochemistry Source Type: research

Improving the yield of recalcitrant Nanobodies® by simple modifications to the standard protocol
Publication date: Available online 12 May 2021Source: Protein Expression and PurificationAuthor(s): Christopher K. Kariuki, Stefan Magez (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - May 13, 2021 Category: Biochemistry Source Type: research

Characterization of Recombinant E. coli Expressing a Novel Fucosidase from Bacillus cereus 2-8 belonging to GH95 Family
Publication date: Available online 12 May 2021Source: Protein Expression and PurificationAuthor(s): Qian Li, Chaofeng Jiang, Haidong Tan, Xiaoming Zhao, Kuikui Li, Heng Yin (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - May 13, 2021 Category: Biochemistry Source Type: research

Expression, purification and characterization of the IcmG and IcmK proteins of the Type IVB secretion system from Coxiella burnetii
Publication date: Available online 11 May 2021Source: Protein Expression and PurificationAuthor(s): Eirini Mathioudaki, Katerina Arvaniti, Cornelia Muenke, Athina Drakonaki, Iosif Vranakis, Myrto Koutantou, Anna Psaroulaki, Hao Xie, Georgios Tsiotis (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - May 12, 2021 Category: Biochemistry Source Type: research

Editorial Board
Publication date: August 2021Source: Protein Expression and Purification, Volume 184Author(s): (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - May 12, 2021 Category: Biochemistry Source Type: research

Protein expression and purification of G-protein coupled receptor kinase 6 (GRK6), toward structure-based drug design and discovery for multiple myeloma
Publication date: Available online 7 May 2021Source: Protein Expression and PurificationAuthor(s): Tien L. Olson, Shangji Zhang, Dillon Labban, Emily Kaschner, Manuel Aceves, Srivatsan Iyer, Domingo Meza, James D. Zook, Eugene Chun, Felicia M. Craciunescu, Wei Liu, Chang-Xin Shi, A. Keith Stewart, Debra T. Hansen, Nathalie Meurice, Petra Fromme (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - May 9, 2021 Category: Biochemistry Source Type: research

Expression and characterization of 1,4-α-glucan branching enzyme from Microvirga sp. MC18 and its application in the preparation of slowly digestible starch
Publication date: Available online 5 May 2021Source: Protein Expression and PurificationAuthor(s): Xianfeng Ye, Wei Liu, Shiyun Ma, Xiaopei Chen, Yan Qiao, Yuqiang Zhao, Qiwen Fan, Xu li, Chaonan Dong, Xiaodong Fang, Minghui Huan, Jian Han, Yan Huang, Zhongli Cui, Zhoukun Li (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - May 6, 2021 Category: Biochemistry Source Type: research

Large scale purification and characterization of A21 deamidated variant-most prominent Post Translational Modification (PTM) for insulins which is also widely observed in insulins pharmaceutical manufacturing and storage
Publication date: Available online 3 May 2021Source: Protein Expression and PurificationAuthor(s): Vibhava Shukla, Koduru Srivatsa, Madhu Kumar, Navratna Vajpai, Neha Agarwal, Nethra S, Somesh B. P, Abhishek Kulshrestha, Partha Hazra (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - May 4, 2021 Category: Biochemistry Source Type: research

Cloning and characterization of a novel DNase gene from Trichogramma pretiosum
Publication date: Available online 1 May 2021Source: Protein Expression and PurificationAuthor(s): Lijuan Yu, Yi Feng, Suxiang Zheng, Jie Li, Ruocen Liu, Dayong Jin (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - May 2, 2021 Category: Biochemistry Source Type: research

Expression and purification of a recombinant ELRL-MAP30 with Dual-targeting anti-tumor bioactivity
Publication date: Available online 30 April 2021Source: Protein Expression and PurificationAuthor(s): Wei-wei Chen, Hong-rui Zhang, Zhi-Guang Huang, Zhe-yue Zhou, Qiu-wen Lou, Xin-yi Jiang, Zhen-hong Zhu (Source: Protein Expression and Purification)
Source: Protein Expression and Purification - May 1, 2021 Category: Biochemistry Source Type: research